SSGCID: Target Page

PsaeA.00935.a

Cysteine desulfurase (EC 2.8.1.7)

CENTER ID:	PsaeA.00935.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.00935.a.B1.GE38253	full length		1	404

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.3993
PGAT:	pseudomonas:PA3814
RefSeq:	NP_252503.1
UniProt:	Q9HXI8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKLPIYLDYS ATTPVDPRVA QKMSECLLMD GNFGNPASRS HVFGWKAEEA VENARRQVAE LVNADPREIV WTSGATESDN LAIKGVAHFY SGKGKHIITS KIEHKAVLDT CRQLEREGFE VTYLEPGEDG LITPALVEAA LRDDTILVSV MHVNNEIGTV NDIAAIGELT RSRGVLFHVD AAQSTGKVEI DLDKLKVDLM SFSAHKTYGP KGIGALYVRR KPRVRIEGQM HGGGHERGMR SGTLATHQIV GMGEAFRIAK EEMAQENARV LALRDRFFAQ IDGLEELYIN GSMTSRVPHN LNVSFNYVEG ESLIMALKDL AVSSGSACTS ASLEPSYVLR ALGRNDELAH SSIRFTFGRF TTEEEIDYAA KKVVEAVSKL RELSPLWDMY KEGVDLSQVE WQAH

NT Sequence

atgaaattgc cgatctacct cgattactcc gcgaccactc ccgtcgatcc ccgcgtcgcc cagaaaatga gcgaatgcct gctgatggac ggcaacttcg gcaacccggc ttcgcgttcc cacgtgttcg gctggaaagc cgaagaagcc gtggagaacg cccgtcgcca ggtggccgag ctggtcaacg ccgatccccg cgagatcgtc tggacctccg gcgccaccga gtccgacaac ctggcgatca agggcgtcgc gcacttctac tcgggcaagg gcaagcacat catcacctcg aagatcgagc acaaggcggt cctcgacacc tgccgccagc tggagcgcga aggtttcgaa gtgacctacc tggagccggg agaagatggc ctgatcactc cggctctggt cgaggcggcg ctgcgtgacg acaccatcct ggtctcggtc atgcacgtga acaacgagat cggcaccgtc aacgacatcg ccgccatcgg cgagctgacc cgctcccgcg gcgtgctgtt ccacgtcgac gccgcacagt cgaccggcaa ggtcgagatc gacctggaca agctcaaggt cgacctgatg tccttctccg cgcacaagac ctacggcccg aaaggcattg gcgcgctcta cgtccggcgc aagccgcgcg tccgcatcga gggtcagatg cacggtggcg gccatgagcg cggcatgcgt tccggcaccc tagccaccca ccagatcgtc ggcatgggcg aggcgttccg catcgccaag gaagaaatgg cccaggagaa cgcccgcgtg ctggcgctcc gcgaccgctt cttcgcgcag atcgacggcc tggaagagct ctacatcaac ggcagcatga cctcccgcgt cccgcacaac ctcaacgtca gcttcaacta cgtcgagggc gagtcgctga tcatggcgct caaggacctc gcggtctcct ccggctcggc ctgtacctcg gcgtccctgg aaccgtccta cgtattgcgc gccctgggcc gcaacgacga actggcgcat agctccatcc gcttcacctt cggtcgcttc accaccgagg aagagatcga ctatgccgcg aagaaggtgg tcgaggccgt ttccaagctg cgcgaactgt cgccgctgtg ggatatgtac aaagagggcg tcgacctgtc ccaggtcgaa tggcaggcgc actga

Details for PsaeA.00935.a.B1.GE38253

HARVESTED ON:	7/1/2014
SEQUENCED ON:	7/8/2014
EXPECTED MW:	46kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

Validated NT Sequence

caggtcgacg ccctctttgt acatatccca cagcggcgac agttcgcgca gcttggaaac ggcctcgacc accttcttcg cggcatagtc gatctcttcc tcggtggtga agcgaccgaa ggtgaagcgg atggagctat gcgccagttc gtcgttgcgg cccagggcgc gcaatacgta ggacggttcc agggacgccg aggtacaggc cgagccggag gagaccgcga ggtccttgag cgccatgatc agcgactcgc cctcgacgta gttgaagctg acgttgaggt tgtgcgggac gcgggaggtc atgctgccgt tgatgtagag ctcttccagg ccgtcgatct gcgcgaagaa gcggtcgcgg agcgccagca cgcgggcgtt ctcctgggcc atttcttcct tggcgatgcg gaacgcctcg cccatgccga cgatctggtg ggtggctagg gtgccggaac gcatgccgcg ctcatggccg ccaccgtgca tctgaccctc gatgcggacg cgcggcttgc gccggacgta gagcgcgcca atgcctttcg ggccgtaggt cttgtgcgcg gagaaggaca tcaggtcgac cttgagcttg tccaggtcga tctcgacctt gccggtcgac tgtgcggcgt cgacgtggaa cagcacgccg cgggagcggg tcagctcgcc gatggcggcg atgtcgttga cggtgccgat ctcgttgttc acgtgcatga ccgagaccag gatggtgtcg tcacgcagcg ccgcctcgac cagagccgga gtgatcaggc catcttctcc cggctccagg taggtcactt cgaaaccttc gcgctccagc tggcggcagg tgtcgaggac cgccttgtgc tcgatcttcg aggtgatgat gtgcttgccc ttgcccgagt agaagtgcgc gacgcccttg atcgccaggt tgtcggactc ggtggcgccg gaggtccaga cgatctcgcg gggatcggcg ttgaccagct cggccacctg gcgacgggcg ttctccacgg cttcttcggc tttccagccg aacacgtggg aacgcgaagc cgggttgccg aagttgccgt ccatcagcag gcattcgctc attttctggg cgacgcgggg atcgacggga gtggtcgcgg agtaatcgag gtagatcggc aatttcat

Expected Protein Sequence

MAHHHHHHMK LPIYLDYSAT TPVDPRVAQK MSECLLMDGN FGNPASRSHV FGWKAEEAVE NARRQVAELV NADPREIVWT SGATESDNLA IKGVAHFYSG KGKHIITSKI EHKAVLDTCR QLEREGFEVT YLEPGEDGLI TPALVEAALR DDTILVSVMH VNNEIGTVND IAAIGELTRS RGVLFHVDAA QSTGKVEIDL DKLKVDLMSF SAHKTYGPKG IGALYVRRKP RVRIEGQMHG GGHERGMRSG TLATHQIVGM GEAFRIAKEE MAQENARVLA LRDRFFAQID GLEELYINGS MTSRVPHNLN VSFNYVEGES LIMALKDLAV SSGSACTSAS LEPSYVLRAL GRNDELAHSS IRFTFGRFTT EEEIDYAAKK VVEAVSKLRE LSPLWDMYKE GVDLSQVEWQ AH

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat tgccgatcta cctcgattac tccgcgacca ctcccgtcga tccccgcgtc gcccagaaaa tgagcgaatg cctgctgatg gacggcaact tcggcaaccc ggcttcgcgt tcccacgtgt tcggctggaa agccgaagaa gccgtggaga acgcccgtcg ccaggtggcc gagctggtca acgccgatcc ccgcgagatc gtctggacct ccggcgccac cgagtccgac aacctggcga tcaagggcgt cgcgcacttc tactcgggca agggcaagca catcatcacc tcgaagatcg agcacaaggc ggtcctcgac acctgccgcc agctggagcg cgaaggtttc gaagtgacct acctggagcc gggagaagat ggcctgatca ctccggctct ggtcgaggcg gcgctgcgtg acgacaccat cctggtctcg gtcatgcacg tgaacaacga gatcggcacc gtcaacgaca tcgccgccat cggcgagctg acccgctccc gcggcgtgct gttccacgtc gacgccgcac agtcgaccgg caaggtcgag atcgacctgg acaagctcaa ggtcgacctg atgtccttct ccgcgcacaa gacctacggc ccgaaaggca ttggcgcgct ctacgtccgg cgcaagccgc gcgtccgcat cgagggtcag atgcacggtg gcggccatga gcgcggcatg cgttccggca ccctagccac ccaccagatc gtcggcatgg gcgaggcgtt ccgcatcgcc aaggaagaaa tggcccagga gaacgcccgc gtgctggcgc tccgcgaccg cttcttcgcg cagatcgacg gcctggaaga gctctacatc aacggcagca tgacctcccg cgtcccgcac aacctcaacg tcagcttcaa ctacgtcgag ggcgagtcgc tgatcatggc gctcaaggac ctcgcggtct cctccggctc ggcctgtacc tcggcgtccc tggaaccgtc ctacgtattg cgcgccctgg gccgcaacga cgaactggcg catagctcca tccgcttcac cttcggtcgc ttcaccaccg aggaagagat cgactatgcc gcgaagaagg tggtcgaggc cgtttccaag ctgcgcgaac tgtcgccgct gtgggatatg tacaaagagg gcgtcgacct gtcccaggtc gaatggcagg cgcactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat