PsaeA.00888.a

Adenylosuccinate synthetase (AMPSase) (AdSS) (EC 6.3.4.4) (IMP--aspartate ligase)

CENTER ID: PsaeA.00888.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
PsaeA.00888.a.B1.GE38252 full length 1 430
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.5171
PGAT: pseudomonas:PA4938
RefSeq: NP_253625.1
UniProt: Q9HUM6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKNVVVLGT QWGDEGKGKI VDLLTEQAAA VVRYQGGHNA GHTLVIDGEK TVLHLIPSGI LREGVQCLIG NGVVLAPDAL LREITKLEEK GVPVRERLRI SPSCPLILSY HVALDQAREK ARGEAKIGTT GRGIGPAYED KVARRGLRVG DLFHRERFAA KLGELLDYHN FVLQHYYKEP AIDFQKTLDE AMEYAELLKP MMADVAATLH DLRKHGKDIM FEGAQGSLLD IDHGTYPYVT SSNTTAGGTA TGSGFGPLYL DYVLGITKAY TTRVGSGPFP TELFDDVGAY LAKRGHEFGA TTGRARRCGW FDAVILRRAI EINSISGLCL TKLDVLDGLD VVRLCVGYKN ADGDVLEAPT DADSYIGLQP VYEEMPGWSE STVGAKTLEE LPANARAYIK RVEELVGAPI DIISTGPDRN ETIILRHPFA
NT Sequence
atgggtaaga atgtcgtagt cctgggcacc caatggggtg atgagggcaa gggcaagatc gtcgacctgc tcaccgagca ggcggctgcc gtagttcgct accagggtgg tcacaacgct ggccacaccc tggtgatcga tggcgagaaa accgtcctgc acctgattcc atcgggcatc ctgcgcgaag gcgtgcagtg cctgatcggc aatggcgtgg tgctggcgcc ggatgcgctg ctgcgcgaaa tcaccaagct ggaagagaag ggcgtgccgg tgcgcgagcg cctgcgcatc agtccttcct gtccgctgat cctgtcctat cacgtcgccc tcgaccaggc gcgcgagaag gcccgcggcg aagccaagat cggcaccacc ggtcgtggca tcggcccggc atacgaggac aaggtggcac gtcgcggcct gcgcgtcggc gacctgttcc accgcgagcg cttcgccgcc aagctgggcg agctgctgga ctaccataac ttcgtcctgc agcactacta caaggagccg gccatcgact tccagaagac cctggacgag gcgatggagt atgccgagct gctcaagccg atgatggccg atgtcgctgc gaccctgcac gacctgcgca agcacggcaa ggacatcatg ttcgaaggcg cccagggatc gctgctggac atcgaccatg gcacctaccc ctacgtgacc agttccaaca ccaccgctgg cggtaccgcc accggttccg gcttcggccc gctgtacctg gactacgtac tgggtatcac caaggcctat accactcgcg tcggctccgg tccgttcccg accgagctgt tcgacgacgt cggcgcctac ctggccaagc gcggccacga gttcggtgcc accaccggtc gcgctcgtcg ctgcggctgg ttcgatgccg tgatcctgcg ccgcgccatc gagatcaaca gcatctccgg cctgtgcctg accaagctcg acgtgctcga tggcctggat gtggtgcgcc tgtgcgtcgg ctacaagaat gccgatggcg atgtgctgga agcgccgacc gatgccgaca gctacatcgg cctgcagccg gtctacgagg aaatgcccgg ctggagcgag tcgaccgtgg gtgcgaagac cctggaagag ttgccggcca acgcccgcgc ctacatcaag cgcgtggaag agctggtggg tgcgccgatc gatatcatct ccaccggtcc ggaccgcaac gagaccatca tcctgcgcca tccattcgcc tga
Details for PsaeA.00888.a.B1.GE38252
HARVESTED ON: 7/1/2014
SEQUENCED ON: 7/8/2014
EXPECTED MW: 48kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 23
PERCENT COVERAGE: 52
Validated AA Sequence
MVDVQQRSLG AFEHDVLAVL AQVVQGRSDI GHHRLEQLGI LHRLVQGLLE VDGRLLVVVL QDEVMVVQQL AQLGGEALAV EQVADAQAAT CHLVLVCRAD ATTGGADLGF AAGLLARLVE GDVIGQDQRT GRTDAQALAH RHALLFQLGD FAQQRIRRQH HAIADQALHA FAQDARWNQV QDGFLAIDHQ GVASVVTTLV ANYGSRLLGE QVDDLALALI TPLGAQDYDI LT
Validated NT Sequence
tggtctcgtt gcggtccgga ccggtggaga tgatatcgat cggcgcaccc accagctctt ccacgcgctt gatgtaggcg cgggcgttgg ccggcaactc ttccagggtc ttcgcaccca cggtcgactc gctccagccg ggcatttcct cgtagaccgg ctgcaggccg atgtagctgt cggcatcggt cggcgcttcc agcacatcgc catcggcatt cttgtagccg acgcacaggc gcaccacatc caggccatcg agcacgtcga gcttggtcag gcacaggccg gagatgctgt tgatctcgat ggcgcggcgc aggatcacgg catcgaacca gccgcagcga cgagcgcgac cggtggtggc accgaactcg tggccgcgct tggccaggta ggcgccgacg tcgtcgaaca gctcggtcgg gaacggaccg gagccgacgc gagtggtata ggccttggtg atacccagta cgtagtccag gtacagcggg ccgaagccgg aaccggtggc ggtaccgcca gcggtggtgt tggaactggt cacgtagggg taggtgccat ggtcgatgtc cagcagcgat ccctgggcgc cttcgaacat gatgtccttg ccgtgcttgc gcaggtcgtg cagggtcgca gcgacatcgg ccatcatcgg cttgagcagc tcggcatact ccatcgcctc gtccagggtc ttctggaagt cgatggccgg ctccttgtag tagtgctgca ggacgaagtt atggtagtcc agcagctcgc ccagcttggc ggcgaagcgc tcgcggtgga acaggtcgcc gacgcgcagg ccgcgacgtg ccaccttgtc ctcgtatgcc gggccgatgc cacgaccggt ggtgccgatc ttggcttcgc cgcgggcctt ctcgcgcgcc tggtcgaggg cgacgtgata ggacaggatc agcggacagg aaggactgat gcgcaggcgc tcgcgcaccg gcacgccctt ctcttccagc ttggtgattt cgcgcagcag cgcatccggc gccagcacca cgccattgcc gatcaggcac tgcacgcctt cgcgcaggat gcccgatgga atcaggtgca ggacggtttt ctcgccatcg atcaccaggg tgtggccagc gttgtgacca ccctggtagc gaactacggc agccgcctgc tcggtgagca ggtcgacgat cttgcccttg ccctcatcac cccattgggt gcccaggact acgacattct taccc
Expected Protein Sequence
MAHHHHHHMG KNVVVLGTQW GDEGKGKIVD LLTEQAAAVV RYQGGHNAGH TLVIDGEKTV LHLIPSGILR EGVQCLIGNG VVLAPDALLR EITKLEEKGV PVRERLRISP SCPLILSYHV ALDQAREKAR GEAKIGTTGR GIGPAYEDKV ARRGLRVGDL FHRERFAAKL GELLDYHNFV LQHYYKEPAI DFQKTLDEAM EYAELLKPMM ADVAATLHDL RKHGKDIMFE GAQGSLLDID HGTYPYVTSS NTTAGGTATG SGFGPLYLDY VLGITKAYTT RVGSGPFPTE LFDDVGAYLA KRGHEFGATT GRARRCGWFD AVILRRAIEI NSISGLCLTK LDVLDGLDVV RLCVGYKNAD GDVLEAPTDA DSYIGLQPVY EEMPGWSEST VGAKTLEELP ANARAYIKRV EELVGAPIDI ISTGPDRNET IILRHPFA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggta agaatgtcgt agtcctgggc acccaatggg gtgatgaggg caagggcaag atcgtcgacc tgctcaccga gcaggcggct gccgtagttc gctaccaggg tggtcacaac gctggccaca ccctggtgat cgatggcgag aaaaccgtcc tgcacctgat tccatcgggc atcctgcgcg aaggcgtgca gtgcctgatc ggcaatggcg tggtgctggc gccggatgcg ctgctgcgcg aaatcaccaa gctggaagag aagggcgtgc cggtgcgcga gcgcctgcgc atcagtcctt cctgtccgct gatcctgtcc tatcacgtcg ccctcgacca ggcgcgcgag aaggcccgcg gcgaagccaa gatcggcacc accggtcgtg gcatcggccc ggcatacgag gacaaggtgg cacgtcgcgg cctgcgcgtc ggcgacctgt tccaccgcga gcgcttcgcc gccaagctgg gcgagctgct ggactaccat aacttcgtcc tgcagcacta ctacaaggag ccggccatcg acttccagaa gaccctggac gaggcgatgg agtatgccga gctgctcaag ccgatgatgg ccgatgtcgc tgcgaccctg cacgacctgc gcaagcacgg caaggacatc atgttcgaag gcgcccaggg atcgctgctg gacatcgacc atggcaccta cccctacgtg accagttcca acaccaccgc tggcggtacc gccaccggtt ccggcttcgg cccgctgtac ctggactacg tactgggtat caccaaggcc tataccactc gcgtcggctc cggtccgttc ccgaccgagc tgttcgacga cgtcggcgcc tacctggcca agcgcggcca cgagttcggt gccaccaccg gtcgcgctcg tcgctgcggc tggttcgatg ccgtgatcct gcgccgcgcc atcgagatca acagcatctc cggcctgtgc ctgaccaagc tcgacgtgct cgatggcctg gatgtggtgc gcctgtgcgt cggctacaag aatgccgatg gcgatgtgct ggaagcgccg accgatgccg acagctacat cggcctgcag ccggtctacg aggaaatgcc cggctggagc gagtcgaccg tgggtgcgaa gaccctggaa gagttgccgg ccaacgcccg cgcctacatc aagcgcgtgg aagagctggt gggtgcgccg atcgatatca tctccaccgg tccggaccgc aacgagacca tcatcctgcg ccatccattc gcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat