SSGCID: Target Page

PsaeA.00846.a

50S ribosomal protein L4

CENTER ID:	PsaeA.00846.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.00846.a.B1.GE38249	full length		1	200

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.4463
PGAT:	pseudomonas:PA4262
RefSeq:	NP_252952.1
UniProt:	Q9HWD6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQLNVNGAQA IEVSERTFGG EFNETLVHQA VVAYMAGGRQ GSKAQKTRSE VSGGGKKPWR QKGTGRARAG TIRSPIWRGG GTTFAAKPRS HEQKLNKKMY RAALRSILAE LVRLDRLVVV ADFAVDAPKT KGLVAKLDTL GLKDVLIVTD GVDENLYLAA RNLAHVDVRD VQGSDPVSLI AYDKVLVTVS AVKKFEELLG

NT Sequence

atgcaattga atgtgaacgg cgctcaggct atcgaagtct ccgagcgtac ctttggtggc gaattcaacg agaccctggt gcaccaggca gtcgtggcct acatggctgg cggccgtcag ggcagcaagg cgcagaagac tcgctccgaa gtctctggtg gtggcaagaa gccgtggcgt cagaagggca ccggtcgtgc tcgcgctggc accatccgta gccccatctg gcgtgggggc ggcaccactt tcgccgctaa accgcgtagt cacgaacaga agctgaacaa gaagatgtac cgcgcagcac tgcgctccat ccttgccgag ctcgttcgtc tggaccgtct ggtcgtcgtt gccgacttcg ctgtcgatgc accgaaaacc aagggcctgg ttgccaagct ggacaccctg ggtctgaaag acgtgctgat cgtgaccgat ggtgtcgacg agaacctgta cctcgctgcg cgcaacctgg cgcacgtcga tgtacgtgat gtccaaggtt ccgaccccgt cagcctgatc gcctacgaca aggtgctggt caccgtgtcc gccgtgaaga agttcgagga gctgctggga tga

Details for PsaeA.00846.a.B1.GE38249

HARVESTED ON:	7/1/2014
SEQUENCED ON:	7/8/2014
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMQ LNVNGAQAIE VSERTFGGEF NETLVHQAVV AYMAGGRQGS KAQKTRSEVS GGGKKPWRQK GTGRARAGTI RSPIWRGGGT TFAAKPRSHE QKLNKKMYRA ALRSILAELV RLDRLVVVAD FAVDAPKTKG LVAKLDTLGL KDVLIVTDGV DENLYLAARN LAHVDVRDVQ GSDPVSLIAY DKVLVTVSAV KKFEELLG

Validated NT Sequence

atggctcacc accaccacca ccatatgcaa ttgaatgtga acggcgctca ggctatcgaa gtctccgagc gtacctttgg tggcgaattc aacgagaccc tggtgcacca ggcagtcgtg gcctacatgg ctggcggccg tcagggcagc aaggcgcaga agactcgctc cgaagtctct ggtggtggca agaagccgtg gcgtcagaag ggcaccggtc gtgctcgcgc tggcaccatc cgtagcccca tctggcgtgg gggcggcacc actttcgccg ctaaaccgcg tagtcacgaa cagaagctga acaagaagat gtaccgcgca gcactgcgct ccatccttgc cgagctcgtt cgtctggacc gtctggtcgt cgttgccgac ttcgctgtcg atgcaccgaa aaccaagggc ctggttgcca agctggacac cctgggtctg aaagacgtgc tgatcgtgac cgatggtgtc gacgagaacc tgtacctcgc tgcgcgcaac ctggcgcacg tcgatgtacg tgatgtccaa ggttccgacc ccgtcagcct gatcgcctac gacaaggtgc tggtcaccgt gtccgccgtg aagaagttcg aggagctgct gggataagtg agtaagatag gatccggctg ctaacnancc cccgaaaga

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaat tgaatgtgaa cggcgctcag gctatcgaag tctccgagcg tacctttggt ggcgaattca acgagaccct ggtgcaccag gcagtcgtgg cctacatggc tggcggccgt cagggcagca aggcgcagaa gactcgctcc gaagtctctg gtggtggcaa gaagccgtgg cgtcagaagg gcaccggtcg tgctcgcgct ggcaccatcc gtagccccat ctggcgtggg ggcggcacca ctttcgccgc taaaccgcgt agtcacgaac agaagctgaa caagaagatg taccgcgcag cactgcgctc catccttgcc gagctcgttc gtctggaccg tctggtcgtc gttgccgact tcgctgtcga tgcaccgaaa accaagggcc tggttgccaa gctggacacc ctgggtctga aagacgtgct gatcgtgacc gatggtgtcg acgagaacct gtacctcgct gcgcgcaacc tggcgcacgt cgatgtacgt gatgtccaag gttccgaccc cgtcagcctg atcgcctacg acaaggtgct ggtcaccgtg tccgccgtga agaagttcga ggagctgctg ggatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat