PsaeA.00820.a
4-hydroxy-tetrahydrodipicolinate reductase (HTPA reductase) (EC 1.17.1.8)
| CENTER ID: | PsaeA.00820.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00820.a.B1.GE38248 | full length | 1 | 268 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00820.a.B1.PW37541 | full length | 1 | 268 |
Structures
4YWJ
DEPOSITED: 3/20/2015
DETERMINATION: XRay
CLONE: PsaeA.00820.a.B1.GE38248
PROTEIN: PsaeA.00820.a.B1.PW37541
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.4985 |
| PGAT: | pseudomonas:PA4759 |
| RefSeq: | NP_253447.1 |
| UniProt: | P38103 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRIAVVGAA GRMGKNLIEA VQQTGGAAGL TAAVDRPDST LVGADAGELA GLGRIGVPLS GDLGKVCEEF DVLIDFTHPS VTLKNIEQCR KARRAMVIGT TGFSADEKLL LAEAAKDIPI VFAANFSVGV NLCLKLLDTA ARVLGDEVDI EIIEAHHRHK VDAPSGTALR MGEVVAQALG RDLQEVAVYG REGQTGARAR ETIGFATVRA GDVVGDHTVL FAAEGERVEI THKASSRMTF ARGAVRAALW LEGKENGLYD MQDVLGLR
NT Sequence
ATGCGACGTA TAGCCGTAGT GGGCGCCGCC GGGCGCATGG GCAAGAACCT GATCGAGGCC GTTCAGCAGA CCGGCGGTGC CGCCGGGCTG ACGGCGGCGG TGGATCGTCC GGATAGCACC CTGGTGGGGG CGGACGCCGG CGAGCTGGCC GGTCTCGGCC GGATCGGCGT GCCGCTGTCC GGGGACCTGG GCAAGGTCTG CGAGGAGTTC GACGTCCTGA TCGACTTCAC CCATCCCTCC GTAACCCTGA AGAACATCGA GCAGTGCCGC AAGGCGCGCC GGGCGATGGT CATCGGCACC ACCGGCTTCA GCGCCGACGA GAAGCTGCTG CTGGCCGAGG CGGCGAAGGA TATCCCGATC GTCTTCGCTG CCAACTTCAG CGTCGGCGTG AACCTTTGCC TGAAGCTTCT GGATACCGCG GCACGGGTGT TGGGCGACGA AGTGGATATC GAGATCATCG AGGCCCATCA CCGGCACAAG GTCGATGCAC CGTCCGGCAC CGCCTTGCGC ATGGGCGAGG TGGTAGCCCA GGCGCTCGGC CGGGACCTCC AGGAAGTGGC GGTCTACGGA CGCGAGGGGC AGACCGGCGC GCGGGCGCGG GAAACCATCG GCTTCGCCAC CGTGCGCGCC GGCGACGTGG TGGGCGACCA CACCGTGCTG TTCGCCGCCG AGGGCGAGCG CGTGGAGATC ACCCACAAGG CTTCCAGCCG CATGACCTTC GCCCGTGGCG CGGTGCGTGC GGCGCTGTGG CTGGAGGGCA AGGAGAACGG CCTGTACGAC ATGCAGGATG TGCTCGGCCT ACGCTGA
Details for PsaeA.00820.a.B1.GE38248
| HARVESTED ON: | 7/1/2014 |
| SEQUENCED ON: | 7/8/2014 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMR RIAVVGAAGR MGKNLIEAVQ QTGGAAGLTA AVDRPDSTLV GADAGELAGL GRIGVPLSGD LGKVCEEFDV LIDFTHPSVT LKNIEQCRKA RRAMVIGTTG FSADEKLLLA EAAKDIPIVF AANFSVGVNL CLKLLDTAAR VLGDEVDIEI IEAHHRHKVD APSGTALRMG EVVAQALGRD LQEVAVYGRE GQTGARARET IGFATVRAGD VVGDHTVLFA AEGERVEITH KASSRMTFAR GAVRAALWLE GKENGLYDMQ DVLGLR
Validated NT Sequence
atcttactca cttagcgtag gccgagcaca tcctgcatgt cgtacaggcc gttctccttg ccctccagcc acagcgccgc acgcaccgcg ccacgggcga aggtcatgcg gctggaagcc ttgtgggtga tctccacgcg ctcgccctcg gcggcgaaca gcacggtgtg gtcgcccacc acgtcgccgg cgcgcacggt ggcgaagccg atggtttccc gcgcccgcgc gccggtctgc ccctcgcgtc cgtagaccgc cacttcctgg aggtcccggc cgagcgcctg ggctaccacc tcgcccatgc gcaaggcggt gccggacggt gcatcgacct tgtgccggtg atgggcctcg atgatctcga tatccacttc gtcgcccaac acccgtgccg cggtatccag aagcttcagg caaaggttca cgccgacgct gaagttggca gcgaagacga tcgggatatc cttcgccgcc tcggccagca gcagcttctc gtcggcgctg aagccggtgg tgccgatgac catcgcccgg cgcgccttgc ggcactgctc gatgttcttc agggttacgg agggatgggt gaagtcgatc aggacgtcga actcctcgca gaccttgccc aggtccccgg acagcggcac gccgatccgg ccgagaccgg ccagctcgcc ggcgtccgcc cccaccaggg tgctatccgg acgatccacc gccgccgtca gcccggcggc accgccggtc tgctgaacgg cctcgatcag gttcttgccc atgcgcccgg cggcgcccac tacggctata cgtcgcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMR RIAVVGAAGR MGKNLIEAVQ QTGGAAGLTA AVDRPDSTLV GADAGELAGL GRIGVPLSGD LGKVCEEFDV LIDFTHPSVT LKNIEQCRKA RRAMVIGTTG FSADEKLLLA EAAKDIPIVF AANFSVGVNL CLKLLDTAAR VLGDEVDIEI IEAHHRHKVD APSGTALRMG EVVAQALGRD LQEVAVYGRE GQTGARARET IGFATVRAGD VVGDHTVLFA AEGERVEITH KASSRMTFAR GAVRAALWLE GKENGLYDMQ DVLGLR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgac gtatagccgt agtgggcgcc gccgggcgca tgggcaagaa cctgatcgag gccgttcagc agaccggcgg tgccgccggg ctgacggcgg cggtggatcg tccggatagc accctggtgg gggcggacgc cggcgagctg gccggtctcg gccggatcgg cgtgccgctg tccggggacc tgggcaaggt ctgcgaggag ttcgacgtcc tgatcgactt cacccatccc tccgtaaccc tgaagaacat cgagcagtgc cgcaaggcgc gccgggcgat ggtcatcggc accaccggct tcagcgccga cgagaagctg ctgctggccg aggcggcgaa ggatatcccg atcgtcttcg ctgccaactt cagcgtcggc gtgaaccttt gcctgaagct tctggatacc gcggcacggg tgttgggcga cgaagtggat atcgagatca tcgaggccca tcaccggcac aaggtcgatg caccgtccgg caccgccttg cgcatgggcg aggtggtagc ccaggcgctc ggccgggacc tccaggaagt ggcggtctac ggacgcgagg ggcagaccgg cgcgcgggcg cgggaaacca tcggcttcgc caccgtgcgc gccggcgacg tggtgggcga ccacaccgtg ctgttcgccg ccgagggcga gcgcgtggag atcacccaca aggcttccag ccgcatgacc ttcgcccgtg gcgcggtgcg tgcggcgctg tggctggagg gcaaggagaa cggcctgtac gacatgcagg atgtgctcgg cctacgctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for PsaeA.00820.a.B1.PW37541
| PURIFICATION DATe: | 8/18/2014 |
| CONCENTRATION: | 45.3mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR RIAVVGAAGR MGKNLIEAVQ QTGGAAGLTA AVDRPDSTLV GADAGELAGL GRIGVPLSGD LGKVCEEFDV LIDFTHPSVT LKNIEQCRKA RRAMVIGTTG FSADEKLLLA EAAKDIPIVF AANFSVGVNL CLKLLDTAAR VLGDEVDIEI IEAHHRHKVD APSGTALRMG EVVAQALGRD LQEVAVYGRE GQTGARARET IGFATVRAGD VVGDHTVLFA AEGERVEITH KASSRMTFAR GAVRAALWLE GKENGLYDMQ DVLGLR
Validated NT Sequence
atcttactca cttagcgtag gccgagcaca tcctgcatgt cgtacaggcc gttctccttg ccctccagcc acagcgccgc acgcaccgcg ccacgggcga aggtcatgcg gctggaagcc ttgtgggtga tctccacgcg ctcgccctcg gcggcgaaca gcacggtgtg gtcgcccacc acgtcgccgg cgcgcacggt ggcgaagccg atggtttccc gcgcccgcgc gccggtctgc ccctcgcgtc cgtagaccgc cacttcctgg aggtcccggc cgagcgcctg ggctaccacc tcgcccatgc gcaaggcggt gccggacggt gcatcgacct tgtgccggtg atgggcctcg atgatctcga tatccacttc gtcgcccaac acccgtgccg cggtatccag aagcttcagg caaaggttca cgccgacgct gaagttggca gcgaagacga tcgggatatc cttcgccgcc tcggccagca gcagcttctc gtcggcgctg aagccggtgg tgccgatgac catcgcccgg cgcgccttgc ggcactgctc gatgttcttc agggttacgg agggatgggt gaagtcgatc aggacgtcga actcctcgca gaccttgccc aggtccccgg acagcggcac gccgatccgg ccgagaccgg ccagctcgcc ggcgtccgcc cccaccaggg tgctatccgg acgatccacc gccgccgtca gcccggcggc accgccggtc tgctgaacgg cctcgatcag gttcttgccc atgcgcccgg cggcgcccac tacggctata cgtcgcatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMR RIAVVGAAGR MGKNLIEAVQ QTGGAAGLTA AVDRPDSTLV GADAGELAGL GRIGVPLSGD LGKVCEEFDV LIDFTHPSVT LKNIEQCRKA RRAMVIGTTG FSADEKLLLA EAAKDIPIVF AANFSVGVNL CLKLLDTAAR VLGDEVDIEI IEAHHRHKVD APSGTALRMG EVVAQALGRD LQEVAVYGRE GQTGARARET IGFATVRAGD VVGDHTVLFA AEGERVEITH KASSRMTFAR GAVRAALWLE GKENGLYDMQ DVLGLR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGAC GTATAGCCGT AGTGGGCGCC GCCGGGCGCA TGGGCAAGAA CCTGATCGAG GCCGTTCAGC AGACCGGCGG TGCCGCCGGG CTGACGGCGG CGGTGGATCG TCCGGATAGC ACCCTGGTGG GGGCGGACGC CGGCGAGCTG GCCGGTCTCG GCCGGATCGG CGTGCCGCTG TCCGGGGACC TGGGCAAGGT CTGCGAGGAG TTCGACGTCC TGATCGACTT CACCCATCCC TCCGTAACCC TGAAGAACAT CGAGCAGTGC CGCAAGGCGC GCCGGGCGAT GGTCATCGGC ACCACCGGCT TCAGCGCCGA CGAGAAGCTG CTGCTGGCCG AGGCGGCGAA GGATATCCCG ATCGTCTTCG CTGCCAACTT CAGCGTCGGC GTGAACCTTT GCCTGAAGCT TCTGGATACC GCGGCACGGG TGTTGGGCGA CGAAGTGGAT ATCGAGATCA TCGAGGCCCA TCACCGGCAC AAGGTCGATG CACCGTCCGG CACCGCCTTG CGCATGGGCG AGGTGGTAGC CCAGGCGCTC GGCCGGGACC TCCAGGAAGT GGCGGTCTAC GGACGCGAGG GGCAGACCGG CGCGCGGGCG CGGGAAACCA TCGGCTTCGC CACCGTGCGC GCCGGCGACG TGGTGGGCGA CCACACCGTG CTGTTCGCCG CCGAGGGCGA GCGCGTGGAG ATCACCCACA AGGCTTCCAG CCGCATGACC TTCGCCCGTG GCGCGGTGCG TGCGGCGCTG TGGCTGGAGG GCAAGGAGAA CGGCCTGTAC GACATGCAGG ATGTGCTCGG CCTACGCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT