SSGCID: Target Page

PsaeA.00743.a

Tryptophan--tRNA ligase (EC 6.1.1.2) (Tryptophanyl-tRNA synthetase) (TrpRS)

CENTER ID:	PsaeA.00743.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.00743.a.B1.GE38247	full length		1	448

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.00743.a.B1.PW37554	full length		1	448

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.4648
PGAT:	pseudomonas:PA4439
RefSeq:	NP_253129.1
UniProt:	Q9HVX6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTRILTGIT PTGTPHLGNY AGAIRPAILA SRRSDVDSFY FLADYHALIK CDDPARIQRS RLEIAATWLA GGLDVERATF YRQSDIPEIP ELTWLLTCVS AKGLLNRAHA YKAAVDRNVE AGEDPDAGVT MGLYSYPVLM AADILMFNAH KIPVGRDQVQ HVEMARDIGQ RFNHLFGNGR EFFVLPEAVI EENVATLPGL DGRKMSKSYD NTIPLFSPSR QLKDAIARIV TDSRAPGEPK DPDSSHLFLL YSAFASAEQV AAFRQELLEG LAWGEAKQRL FQLLDNELGE ARERYQALIA KPDDIEDILL AGAAKARRIA TPFIAELREA VGLRSLREPL KSAESGKKKA AKAARLVSFR DDDGSFRFRL LDAAGEQLLL SRAFADGKAA GAVSKRLLAG ETADLRAEGN AFGLWLDGEA VAQSPAFADA AARDAAIERT REALAPQE

NT Sequence

atgactactc gtatcctgac cggcatcact cctaccggca ctccgcacct gggcaactac gccggcgcca tccgcccggc gatcctcgcc agccgtcgga gcgacgtgga ttcgttctac ttcctggcgg actaccacgc cttgatcaag tgcgacgatc ccgctcgtat ccagcgctcg cgcctggaaa tcgccgcgac ctggctggcc ggcggcctgg acgtcgagcg tgcgaccttc taccggcagt cggacatccc cgagattccc gagctgacct ggttgctcac ctgcgtcagt gccaagggcc tgctcaaccg cgcgcatgcc tacaaggccg cggtggaccg caatgtcgag gccggcgaag acccggacgc cggcgtgacc atgggcctct acagctatcc ggtgctgatg gccgcggaca tcctgatgtt caacgcgcac aagattccgg tcggccgcga ccaggtgcag cacgtggaga tggcccgcga catcggccag cgtttcaacc acctgttcgg caacggccgc gagttcttcg tcctgcccga agcggtgatc gaggaaaacg tcgccaccct gccgggcctg gacgggcgca agatgtccaa gagctacgac aacaccatcc cgctgttcag cccgtccagg cagctcaagg acgccatcgc acgcatcgtc accgactcgc gcgcgccggg cgagccgaaa gacccggaca gttcgcacct gttccttctt tactcggcct tcgccagcgc cgaacaggtc gccgcgttcc gccaggaact gctcgaaggc ctggcctggg gcgaagccaa gcagcgcctg ttccagttgc tcgacaacga actgggcgag gctcgcgagc gttaccaggc gctgatcgcc aagccggacg atatcgagga cattctcctc gcaggtgccg ccaaggcgcg ccgcatcgcc accccattca tcgccgagtt gcgcgaggca gtcggcctgc gctccctgcg cgagccgctg aaaagtgccg agagcggcaa gaagaaggcc gccaaggctg cgcgcctggt cagcttccgc gacgacgacg gcagcttccg cttccgcctg ctggacgccg ccggcgaaca gctactgctg tcccgcgcct tcgccgacgg caaggccgcc ggtgcggtga gcaagcgcct gctggctggc gagactgccg acctgcgggc cgagggcaat gcctttggcc tgtggctgga tggcgaggcg gtggcgcaga gcccggcctt cgccgacgcc gccgcccgtg acgccgccat cgagcgcacc cgcgaagcgc tggcaccgca ggagtag

Details for PsaeA.00743.a.B1.GE38247

HARVESTED ON:	7/1/2014
SEQUENCED ON:	7/8/2014
EXPECTED MW:	50kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	67

Validated AA Sequence

MGLYSYPVLM AADILMFNAH KIPVGRDQVQ HVEMARDIGQ RFNHLFGNGR EFFVLPEAVI EENVATLPGL DGRKMSKSYD NTIPLFSPSR QLKDAIARIV TDSRAPGEPK DPDSSHLFLL YSAFASAEQV AAFRQELLEG LAWGEAKQRL FQLLDNELGE ARERYQALIA KPDDIEDILL AGAAKARRIA TPFIAELREA VGLRSLREPL KSAESGKKKA AKAARLVSFR DDDGSFRFRL LDAAGEQLLL SRAFADGKAA GAVSKRLLAG ETADLRAEGN AFGLWLDGEA VAQSPAFADA AARDAAIER

Validated NT Sequence

gtgcgctcga tggcggcgtc acgggcggcg gcgtcggcga aggccgggct ctgcgccacc gcctcgccat ccagccacag gccaaaggca ttgccctcgg cccgcaggtc ggcagtctcg ccagccagca ggcgcttgct caccgcaccg gcggccttgc cgtcggcgaa ggcgcgggac agcagtagct gttcgccggc ggcgtccagc aggcggaagc ggaagctgcc gtcgtcgtcg cggaagctga ccaggcgcgc agccttggcg gccttcttct tgccgctctc ggcacttttc agcggctcgc gcagggagcg caggccgact gcctcgcgca actcggcgat gaatggggtg gcgatgcggc gcgccttggc ggcacctgcg aggagaatgt cctcgatatc gtccggcttg gcgatcagcg cctggtaacg ctcgcgagcc tcgcccagtt cgttgtcgag caactggaac aggcgctgct tggcttcgcc ccaggccagg ccttcgagca gttcctggcg gaacgcggcg acctgttcgg cgctggcgaa ggccgagtaa agaaggaaca ggtgcgaact gtccgggtct ttcggctcgc ccggcgcgcg cgagtcggtg acgatgcgtg cgatggcgtc cttgagctgc ctggacgggc tgaacagcgg gatggtgttg tcgtagctct tggacatctt gcgcccgtcc aggcccggca gggtggcgac gttttcctcg atcaccgctt cgggcaggac gaagaactcg cggccgttgc cgaacaggtg gttgaaacgc tggccgatgt cgcgggccat ctccacgtgc tgcacctggt cgcggccgac cggaatcttg tgcgcgttga acatcaggat gtccgcggcc atcagcaccg gatagctgta gaggcccatg gtcacgccgg cgtccgggtc ttcgccggcc tcgacattgc ggtccaccgc ggccttgtag gcatgcgcgc ggttgagcag gcccttggca ctgacgcagg tgagcaacca ggtcagctcg ggaatctcgg ggatgtccga ctgccggtag aaggtcgcac gctcgacgtc caggccgccg gccagccagg tcgcggcgat ttccaggcgc gagcgctgga tacgagcggg atcgtcgcac ttgatcaagg cgtggtagtc cgccaggaag tagaacgaat ccacgtcgct ccgacggctg gcgaggatcg ccgggcggat ggcgccggcg tagttgccca ggtgcggagt gccggtagga gtgatgccgg tcaggatacg agta

Expected Protein Sequence

MAHHHHHHMT TRILTGITPT GTPHLGNYAG AIRPAILASR RSDVDSFYFL ADYHALIKCD DPARIQRSRL EIAATWLAGG LDVERATFYR QSDIPEIPEL TWLLTCVSAK GLLNRAHAYK AAVDRNVEAG EDPDAGVTMG LYSYPVLMAA DILMFNAHKI PVGRDQVQHV EMARDIGQRF NHLFGNGREF FVLPEAVIEE NVATLPGLDG RKMSKSYDNT IPLFSPSRQL KDAIARIVTD SRAPGEPKDP DSSHLFLLYS AFASAEQVAA FRQELLEGLA WGEAKQRLFQ LLDNELGEAR ERYQALIAKP DDIEDILLAG AAKARRIATP FIAELREAVG LRSLREPLKS AESGKKKAAK AARLVSFRDD DGSFRFRLLD AAGEQLLLSR AFADGKAAGA VSKRLLAGET ADLRAEGNAF GLWLDGEAVA QSPAFADAAA RDAAIERTRE ALAPQE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta ctcgtatcct gaccggcatc actcctaccg gcactccgca cctgggcaac tacgccggcg ccatccgccc ggcgatcctc gccagccgtc ggagcgacgt ggattcgttc tacttcctgg cggactacca cgccttgatc aagtgcgacg atcccgctcg tatccagcgc tcgcgcctgg aaatcgccgc gacctggctg gccggcggcc tggacgtcga gcgtgcgacc ttctaccggc agtcggacat ccccgagatt cccgagctga cctggttgct cacctgcgtc agtgccaagg gcctgctcaa ccgcgcgcat gcctacaagg ccgcggtgga ccgcaatgtc gaggccggcg aagacccgga cgccggcgtg accatgggcc tctacagcta tccggtgctg atggccgcgg acatcctgat gttcaacgcg cacaagattc cggtcggccg cgaccaggtg cagcacgtgg agatggcccg cgacatcggc cagcgtttca accacctgtt cggcaacggc cgcgagttct tcgtcctgcc cgaagcggtg atcgaggaaa acgtcgccac cctgccgggc ctggacgggc gcaagatgtc caagagctac gacaacacca tcccgctgtt cagcccgtcc aggcagctca aggacgccat cgcacgcatc gtcaccgact cgcgcgcgcc gggcgagccg aaagacccgg acagttcgca cctgttcctt ctttactcgg ccttcgccag cgccgaacag gtcgccgcgt tccgccagga actgctcgaa ggcctggcct ggggcgaagc caagcagcgc ctgttccagt tgctcgacaa cgaactgggc gaggctcgcg agcgttacca ggcgctgatc gccaagccgg acgatatcga ggacattctc ctcgcaggtg ccgccaaggc gcgccgcatc gccaccccat tcatcgccga gttgcgcgag gcagtcggcc tgcgctccct gcgcgagccg ctgaaaagtg ccgagagcgg caagaagaag gccgccaagg ctgcgcgcct ggtcagcttc cgcgacgacg acggcagctt ccgcttccgc ctgctggacg ccgccggcga acagctactg ctgtcccgcg ccttcgccga cggcaaggcc gccggtgcgg tgagcaagcg cctgctggct ggcgagactg ccgacctgcg ggccgagggc aatgcctttg gcctgtggct ggatggcgag gcggtggcgc agagcccggc cttcgccgac gccgccgccc gtgacgccgc catcgagcgc acccgcgaag cgctggcacc gcaggagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for PsaeA.00743.a.B1.PW37554

PURIFICATION DATe:	9/9/2014
CONCENTRATION:	44mg/ml
OBSERVED MW:	48kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	67

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTA CTCGTATCCT GACCGGCATC ACTCCTACCG GCACTCCGCA CCTGGGCAAC TACGCCGGCG CCATCCGCCC GGCGATCCTC GCCAGCCGTC GGAGCGACGT GGATTCGTTC TACTTCCTGG CGGACTACCA CGCCTTGATC AAGTGCGACG ATCCCGCTCG TATCCAGCGC TCGCGCCTGG AAATCGCCGC GACCTGGCTG GCCGGCGGCC TGGACGTCGA GCGTGCGACC TTCTACCGGC AGTCGGACAT CCCCGAGATT CCCGAGCTGA CCTGGTTGCT CACCTGCGTC AGTGCCAAGG GCCTGCTCAA CCGCGCGCAT GCCTACAAGG CCGCGGTGGA CCGCAATGTC GAGGCCGGCG AAGACCCGGA CGCCGGCGTG ACCATGGGCC TCTACAGCTA TCCGGTGCTG ATGGCCGCGG ACATCCTGAT GTTCAACGCG CACAAGATTC CGGTCGGCCG CGACCAGGTG CAGCACGTGG AGATGGCCCG CGACATCGGC CAGCGTTTCA ACCACCTGTT CGGCAACGGC CGCGAGTTCT TCGTCCTGCC CGAAGCGGTG ATCGAGGAAA ACGTCGCCAC CCTGCCGGGC CTGGACGGGC GCAAGATGTC CAAGAGCTAC GACAACACCA TCCCGCTGTT CAGCCCGTCC AGGCAGCTCA AGGACGCCAT CGCACGCATC GTCACCGACT CGCGCGCGCC GGGCGAGCCG AAAGACCCGG ACAGTTCGCA CCTGTTCCTT CTTTACTCGG CCTTCGCCAG CGCCGAACAG GTCGCCGCGT TCCGCCAGGA ACTGCTCGAA GGCCTGGCCT GGGGCGAAGC CAAGCAGCGC CTGTTCCAGT TGCTCGACAA CGAACTGGGC GAGGCTCGCG AGCGTTACCA GGCGCTGATC GCCAAGCCGG ACGATATCGA GGACATTCTC CTCGCAGGTG CCGCCAAGGC GCGCCGCATC GCCACCCCAT TCATCGCCGA GTTGCGCGAG GCAGTCGGCC TGCGCTCCCT GCGCGAGCCG CTGAAAAGTG CCGAGAGCGG CAAGAAGAAG GCCGCCAAGG CTGCGCGCCT GGTCAGCTTC CGCGACGACG ACGGCAGCTT CCGCTTCCGC CTGCTGGACG CCGCCGGCGA ACAGCTACTG CTGTCCCGCG CCTTCGCCGA CGGCAAGGCC GCCGGTGCGG TGAGCAAGCG CCTGCTGGCT GGCGAGACTG CCGACCTGCG GGCCGAGGGC AATGCCTTTG GCCTGTGGCT GGATGGCGAG GCGGTGGCGC AGAGCCCGGC CTTCGCCGAC GCCGCCGCCC GTGACGCCGC CATCGAGCGC ACCCGCGAAG CGCTGGCACC GCAGGAGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT