PsaeA.00612.a
Lysine--tRNA ligase (EC 6.1.1.6) (Lysyl-tRNA synthetase) (LysRS)
| CENTER ID: | PsaeA.00612.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00612.a.B1.GE38243 | full length | 1 | 501 | ||
| PsaeA.00612.a.B21.GE40491 | 14-501 | 14 | 501 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00612.a.B1.PS02177 | full length | 1 | 501 | ||
| PsaeA.00612.a.B21.PD00477 | 14-501 | 14 | 501 |
Structures
36LW
DEPOSITED: 6/17/2026
DETERMINATION: XRay
CLONE: PsaeA.00612.a.B3.GE45670
PROTEIN: PsaeA.00612.a.B3.PW39509
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|208964.12.peg.3870 |
| PGAT: | pseudomonas:PA3700 |
| RefSeq: | NP_252390.1 |
| UniProt: | Q9HXU0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDQQLDQHE LQQEENKLIA QRKEKLAAVR EARAIAFPND FRRDAYFADL QKQYADKTKE ELEAAAIPVK VAGRIMLNRG SFIVLQDSSE RLQVYVNRKT LPEETLAEIK TWDLGDIIGA EGVLARSGKG DLYVDMTSVR LLTKSLRPLP DKHHGLTDTE QRYRQRYVDL MVNEETRHTF RVRSQVIAHI RRFLSERGFL EVETPMLQTI PGGAAAKPFE THHNALDMAM FLRIAPELYL KRLVVGGFEK VFEINRNFRN EGVSTRHNPE FTMLEFYQAY ADYEDNMDLT EELFRELAQS VLGTTDVPYG DKVFHFGEPF VRLSVFDSIL KYNPEITAAD LNDVEKARAI AKKAGAKVLG HEGLGKLQVM IFEELVEHKL EQPHFITRYP FEVSPLARRN DEDPSVTDRF ELFIGGREIA NAYSELNDAE DQAERFMLQV KEKDAGDDEA MHYDADFINA LEYGMPPTAG EGIGIDRLVM LLTNSPSIRD VILFPHMRPQ A
NT Sequence
atgagcgacc aacaactcga ccagcacgaa ctgcaacagg aagaaaacaa gctgatcgcc cagcgcaagg aaaaacttgc cgccgtgcgc gaagcccggg ccatcgcctt ccccaacgac ttccgccgcg acgcctactt cgcggacctg cagaaacagt acgccgacaa gaccaaggaa gagctggaag ccgcagcgat cccggtcaag gtggccggtc ggatcatgct caaccgcggt tccttcatcg tcctccagga cagcagcgag cgcctgcagg tctacgtcaa ccgcaagacc ctgccggaag aaaccctggc cgagatcaag acctgggacc tgggcgacat catcggcgcc gaaggcgtgc tggcccgctc gggcaagggc gacctgtacg tcgacatgac cagcgtgcgc ctgctgacca agtcgctgcg tccgctgccg gacaagcacc atggcctgac cgacaccgag cagcgctacc gccagcgcta cgtcgacctg atggtcaacg aggaaacccg ccacaccttc cgcgtgcgtt cccaggtcat cgcccacatc cgccgtttcc tcagcgagcg cggtttcctc gaagtggaga ccccgatgct gcagaccatc cccggcggcg ccgcggccaa gccgttcgag acccatcaca acgcgctgga catggccatg ttcctgcgca tcgccccgga gctgtacctc aagcgcctgg tggtcggcgg tttcgagaag gtcttcgaga tcaaccgcaa cttccgtaac gaaggcgtct cgacccggca caaccccgag ttcaccatgc tcgagttcta ccaggcctac gccgactacg aagacaacat ggacctgacc gaggaactgt tccgcgaact ggcccagtcg gtactcggta ccaccgacgt gccctacggc gacaaggtct tccacttcgg cgagccgttc gtgcgcctgt cggtgttcga ttcgatcctc aagtacaacc cggagatcac cgccgccgac ctcaacgacg tcgagaaggc gcgggccatc gccaagaagg ccggcgccaa ggtcctcggc cacgaaggcc tgggcaagct gcaggtgatg attttcgagg aactggtgga gcacaagctg gagcagccgc acttcatcac ccgctacccc ttcgaggttt cgccgctggc ccgccgcaac gacgaagacc cgagcgtcac cgaccgcttc gagctgttca tcggcggccg cgagatcgcc aatgcctatt ccgagctgaa cgacgccgag gaccaggccg agcgcttcat gttgcaggtc aaggagaagg acgccggcga cgacgaggcg atgcactacg acgccgactt catcaatgcc ctggaatacg gcatgccgcc gaccgccggc gaaggcatcg gcatcgaccg cctggtgatg ctgctgacca actcgccgtc gatccgcgac gtgatcctgt tcccgcacat gcgcccgcag gcctga
Details for PsaeA.00612.a.B1.GE38243
| HARVESTED ON: | 7/1/2014 |
| SEQUENCED ON: | 7/8/2014 |
| EXPECTED MW: | 58kDa |
| OBSERVED MW: | 66kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 81 |
Validated AA Sequence
MLNRGSFIVL QDSSERLQVY VNRKTLPEET LAEIKTWDLG DIIGAEGVLA RSGKGDLYVD MTSVRLLTKS LRPLPDKHHG LTDTEQRYRQ RYVDLMVNEE TRHTFRVRSQ VIAHIRRFLS ERGFLEVETP MLQTIPGGAA AKPFETHHNA LDMAMFLRIA PELYLKRLVV GGFEKVFEIN RNFRNEGVST RHNPEFTMLE FYQAYADYED NMDLTEELFR ELAQSVLGTT DVPYGDKVFH FGEPFVRLSV FDSILKYNPE ITAADLNDVE KARAIAKKAG AKVLGHEGLG KLQVMIFEEL VEHKLEQPHF ITRYPFEVSP LARRNDEDPS VTDRFELFIG GREIANAYSE LNDAEDQAER FMLQVKEKDA GDDEAMHYDA DFINALEYGM PPTAGEGIGI DRLVMLLTNS PSIRDVI
Validated NT Sequence
caggatcacg tcgcggatcg acggcgagtt ggtcagcagc atcaccaggc ggtcgatgcc gatgccttcg ccggcggtcg gcggcatgcc gtattccagg gcattgatga agtcggcgtc gtagtgcatc gcctcgtcgt cgccggcgtc cttctccttg acctgcaaca tgaagcgctc ggcctggtcc tcggcgtcgt tcagctcgga ataggcattg gcgatctcgc ggccgccgat gaacagctcg aagcggtcgg tgacgctcgg gtcttcgtcg ttgcggcggg ccagcggcga aacctcgaag gggtagcggg tgatgaagtg cggctgctcc agcttgtgct ccaccagttc ctcgaaaatc atcacctgca gcttgcccag gccttcgtgg ccgaggacct tggcgccggc cttcttggcg atggcccgcg ccttctcgac gtcgttgagg tcggcggcgg tgatctccgg gttgtacttg aggatcgaat cgaacaccga caggcgcacg aacggctcgc cgaagtggaa gaccttgtcg ccgtagggca cgtcggtggt accgagtacc gactgggcca gttcgcggaa cagttcctcg gtcaggtcca tgttgtcttc gtagtcggcg taggcctggt agaactcgag catggtgaac tcggggttgt gccgggtcga gacgccttcg ttacggaagt tgcggttgat ctcgaagacc ttctcgaaac cgccgaccac caggcgcttg aggtacagct ccggggcgat gcgcaggaac atggccatgt ccagcgcgtt gtgatgggtc tcgaacggct tggccgcggc gccgccgggg atggtctgca gcatcggggt ctccacttcg aggaaaccgc gctcgctgag gaaacggcgg atgtgggcga tgacctggga acgcacgcgg aaggtgtggc gggtttcctc gttgaccatc aggtcgacgt agcgctggcg gtagcgctgc tcggtgtcgg tcaggccatg gtgcttgtcc ggcagcggac gcagcgactt ggtcagcagg cgcacgctgg tcatgtcgac gtacaggtcg cccttgcccg agcgggccag cacgccttcg gcgccgatga tgtcgcccag gtcccaggtc ttgatctcgg ccagggtttc ttccggcagg gtcttgcggt tgacgtagac ctgcaggcgc tcgctgctgt cctggaggac gatgaaggaa ccgcggttga gcatgatccg accggccacc ttgaccggga tcgctgcggc ttccagctct tccttggtct tgtcggcgta ctgtttctgc aggtccgcga agtaggcgtc gcggcggaag tcgttgggga aggcgatggc ccgggcttcg cgcacggcgg caagtttttc cttgcgctgg gcgatcagct tgttttcttc ctgttgcagt tcgtgctggt cgagttgttg gtcgctca
Expected Protein Sequence
MAHHHHHHMS DQQLDQHELQ QEENKLIAQR KEKLAAVREA RAIAFPNDFR RDAYFADLQK QYADKTKEEL EAAAIPVKVA GRIMLNRGSF IVLQDSSERL QVYVNRKTLP EETLAEIKTW DLGDIIGAEG VLARSGKGDL YVDMTSVRLL TKSLRPLPDK HHGLTDTEQR YRQRYVDLMV NEETRHTFRV RSQVIAHIRR FLSERGFLEV ETPMLQTIPG GAAAKPFETH HNALDMAMFL RIAPELYLKR LVVGGFEKVF EINRNFRNEG VSTRHNPEFT MLEFYQAYAD YEDNMDLTEE LFRELAQSVL GTTDVPYGDK VFHFGEPFVR LSVFDSILKY NPEITAADLN DVEKARAIAK KAGAKVLGHE GLGKLQVMIF EELVEHKLEQ PHFITRYPFE VSPLARRNDE DPSVTDRFEL FIGGREIANA YSELNDAEDQ AERFMLQVKE KDAGDDEAMH YDADFINALE YGMPPTAGEG IGIDRLVMLL TNSPSIRDVI LFPHMRPQA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg accaacaact cgaccagcac gaactgcaac aggaagaaaa caagctgatc gcccagcgca aggaaaaact tgccgccgtg cgcgaagccc gggccatcgc cttccccaac gacttccgcc gcgacgccta cttcgcggac ctgcagaaac agtacgccga caagaccaag gaagagctgg aagccgcagc gatcccggtc aaggtggccg gtcggatcat gctcaaccgc ggttccttca tcgtcctcca ggacagcagc gagcgcctgc aggtctacgt caaccgcaag accctgccgg aagaaaccct ggccgagatc aagacctggg acctgggcga catcatcggc gccgaaggcg tgctggcccg ctcgggcaag ggcgacctgt acgtcgacat gaccagcgtg cgcctgctga ccaagtcgct gcgtccgctg ccggacaagc accatggcct gaccgacacc gagcagcgct accgccagcg ctacgtcgac ctgatggtca acgaggaaac ccgccacacc ttccgcgtgc gttcccaggt catcgcccac atccgccgtt tcctcagcga gcgcggtttc ctcgaagtgg agaccccgat gctgcagacc atccccggcg gcgccgcggc caagccgttc gagacccatc acaacgcgct ggacatggcc atgttcctgc gcatcgcccc ggagctgtac ctcaagcgcc tggtggtcgg cggtttcgag aaggtcttcg agatcaaccg caacttccgt aacgaaggcg tctcgacccg gcacaacccc gagttcacca tgctcgagtt ctaccaggcc tacgccgact acgaagacaa catggacctg accgaggaac tgttccgcga actggcccag tcggtactcg gtaccaccga cgtgccctac ggcgacaagg tcttccactt cggcgagccg ttcgtgcgcc tgtcggtgtt cgattcgatc ctcaagtaca acccggagat caccgccgcc gacctcaacg acgtcgagaa ggcgcgggcc atcgccaaga aggccggcgc caaggtcctc ggccacgaag gcctgggcaa gctgcaggtg atgattttcg aggaactggt ggagcacaag ctggagcagc cgcacttcat cacccgctac cccttcgagg tttcgccgct ggcccgccgc aacgacgaag acccgagcgt caccgaccgc ttcgagctgt tcatcggcgg ccgcgagatc gccaatgcct attccgagct gaacgacgcc gaggaccagg ccgagcgctt catgttgcag gtcaaggaga aggacgccgg cgacgacgag gcgatgcact acgacgccga cttcatcaat gccctggaat acggcatgcc gccgaccgcc ggcgaaggca tcggcatcga ccgcctggtg atgctgctga ccaactcgcc gtcgatccgc gacgtgatcc tgttcccgca catgcgcccg caggcctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for PsaeA.00612.a.B21.GE40491
| HARVESTED ON: | 8/10/2016 |
| SEQUENCED ON: | 8/11/2016 |
| EXPECTED MW: | 57kDa |
| OBSERVED MW: | 57kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHEE NKLIAQRKEK LAAVREARAI AFPNDFRRDA YFADLQKQYA DKTKEELEAA AIPVKVAGRI MLNRGSFIVL QDSSERLQVY VNRKTLPEET LAEIKTWDLG DIIGAEGVLA RSGKGDLYVD MTSVRLLTKS LRPLPDKHHG LTDTEQRYRQ RYVDLMVNEE TRHTFRVRSQ VIAHIRRFLS ERGFLEVETP MLQTIPGGAA AKPFETHHNA LDMAMFLRIA PELYLKRLVV GGFEKVFEIN RNFRNEGVST RHNPEFTMLE FYQAYADYED NMDLTEELFR ELAQSVLGTT DVPYGDKVFH FGEPFVRLSV FDSILKYNPE ITAADLNDVE KARAIAKKAG AKVLGHEGLG KLQVMIFEEL VEHKLEQPHF ITRYPFEVSP LARRNDEDPS VTDRFELFIG GREIANAYSE LNDAEDQAER FMLQVKEKDA GDDEAMHYDA DFINALEYGM PPTAGEGIGI DRLVMLLTNS PSIRDVILFP HMRPQA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaagaaa acaagctgat cgcccagcgc aaggaaaaac ttgccgccgt gcgcgaagcc cgggccatcg ccttccccaa cgacttccgc cgcgacgcct acttcgcgga cctgcagaaa cagtacgccg acaagaccaa ggaagagctg gaagccgcag cgatcccggt caaggtggcc ggtcggatca tgctcaaccg cggttccttc atcgtcctcc aggacagcag cgagcgcctg caggtctacg tcaaccgcaa gaccctgccg gaagaaaccc tggccgagat caagacctgg gacctgggcg acatcatcgg cgccgaaggc gtgctggccc gctcgggcaa gggcgacctg tacgtcgaca tgaccagcgt gcgcctgctg accaagtcgc tgcgtccgct gccggacaag caccatggcc tgaccgacac cgagcagcgc taccgccagc gctacgtcga cctgatggtc aacgaggaaa cccgccacac cttccgcgtg cgttcccagg tcatcgccca catccgccgt ttcctcagcg agcgcggttt cctcgaagtg gagaccccga tgctgcagac catccccggc ggcgccgcgg ccaagccgtt cgagacccat cacaacgcgc tggacatggc catgttcctg cgcatcgccc cggagctgta cctcaagcgc ctggtggtcg gcggtttcga gaaggtcttc gagatcaacc gcaacttccg taacgaaggc gtctcgaccc ggcacaaccc cgagttcacc atgctcgagt tctaccaggc ctacgccgac tacgaagaca acatggacct gaccgaggaa ctgttccgcg aactggccca gtcggtactc ggtaccaccg acgtgcccta cggcgacaag gtcttccact tcggcgagcc gttcgtgcgc ctgtcggtgt tcgattcgat cctcaagtac aacccggaga tcaccgccgc cgacctcaac gacgtcgaga aggcgcgggc catcgccaag aaggccggcg ccaaggtcct cggccacgaa ggcctgggca agctgcaggt gatgattttc gaggaactgg tggagcacaa gctggagcag ccgcacttca tcacccgcta ccccttcgag gtttcgccgc tggcccgccg caacgacgaa gacccgagcg tcaccgaccg cttcgagctg ttcatcggcg gccgcgagat cgccaatgcc tattccgagc tgaacgacgc cgaggaccag gccgagcgct tcatgttgca ggtcaaggag aaggacgccg gcgacgacga ggcgatgcac tacgacgccg acttcatcaa tgccctggaa tacggcatgc cgccgaccgc cggcgaaggc atcggcatcg accgcctggt gatgctgctg accaactcgc cgtcgatccg cgacgtgatc ctgttcccgc acatgcgccc gcaggcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for PsaeA.00612.a.B1.PS02177
| PURIFICATION DATe: | 9/25/2014 |
| CONCENTRATION: | 29.9mg/ml |
| OBSERVED MW: | 64kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 81 |
Protocol Notes
notes unavailable
Validated AA Sequence
MLNRGSFIVL QDSSERLQVY VNRKTLPEET LAEIKTWDLG DIIGAEGVLA RSGKGDLYVD MTSVRLLTKS LRPLPDKHHG LTDTEQRYRQ RYVDLMVNEE TRHTFRVRSQ VIAHIRRFLS ERGFLEVETP MLQTIPGGAA AKPFETHHNA LDMAMFLRIA PELYLKRLVV GGFEKVFEIN RNFRNEGVST RHNPEFTMLE FYQAYADYED NMDLTEELFR ELAQSVLGTT DVPYGDKVFH FGEPFVRLSV FDSILKYNPE ITAADLNDVE KARAIAKKAG AKVLGHEGLG KLQVMIFEEL VEHKLEQPHF ITRYPFEVSP LARRNDEDPS VTDRFELFIG GREIANAYSE LNDAEDQAER FMLQVKEKDA GDDEAMHYDA DFINALEYGM PPTAGEGIGI DRLVMLLTNS PSIRDVI
Validated NT Sequence
caggatcacg tcgcggatcg acggcgagtt ggtcagcagc atcaccaggc ggtcgatgcc gatgccttcg ccggcggtcg gcggcatgcc gtattccagg gcattgatga agtcggcgtc gtagtgcatc gcctcgtcgt cgccggcgtc cttctccttg acctgcaaca tgaagcgctc ggcctggtcc tcggcgtcgt tcagctcgga ataggcattg gcgatctcgc ggccgccgat gaacagctcg aagcggtcgg tgacgctcgg gtcttcgtcg ttgcggcggg ccagcggcga aacctcgaag gggtagcggg tgatgaagtg cggctgctcc agcttgtgct ccaccagttc ctcgaaaatc atcacctgca gcttgcccag gccttcgtgg ccgaggacct tggcgccggc cttcttggcg atggcccgcg ccttctcgac gtcgttgagg tcggcggcgg tgatctccgg gttgtacttg aggatcgaat cgaacaccga caggcgcacg aacggctcgc cgaagtggaa gaccttgtcg ccgtagggca cgtcggtggt accgagtacc gactgggcca gttcgcggaa cagttcctcg gtcaggtcca tgttgtcttc gtagtcggcg taggcctggt agaactcgag catggtgaac tcggggttgt gccgggtcga gacgccttcg ttacggaagt tgcggttgat ctcgaagacc ttctcgaaac cgccgaccac caggcgcttg aggtacagct ccggggcgat gcgcaggaac atggccatgt ccagcgcgtt gtgatgggtc tcgaacggct tggccgcggc gccgccgggg atggtctgca gcatcggggt ctccacttcg aggaaaccgc gctcgctgag gaaacggcgg atgtgggcga tgacctggga acgcacgcgg aaggtgtggc gggtttcctc gttgaccatc aggtcgacgt agcgctggcg gtagcgctgc tcggtgtcgg tcaggccatg gtgcttgtcc ggcagcggac gcagcgactt ggtcagcagg cgcacgctgg tcatgtcgac gtacaggtcg cccttgcccg agcgggccag cacgccttcg gcgccgatga tgtcgcccag gtcccaggtc ttgatctcgg ccagggtttc ttccggcagg gtcttgcggt tgacgtagac ctgcaggcgc tcgctgctgt cctggaggac gatgaaggaa ccgcggttga gcatgatccg accggccacc ttgaccggga tcgctgcggc ttccagctct tccttggtct tgtcggcgta ctgtttctgc aggtccgcga agtaggcgtc gcggcggaag tcgttgggga aggcgatggc ccgggcttcg cgcacggcgg caagtttttc cttgcgctgg gcgatcagct tgttttcttc ctgttgcagt tcgtgctggt cgagttgttg gtcgctca
Expressed Protein Sequence
MAHHHHHHMS DQQLDQHELQ QEENKLIAQR KEKLAAVREA RAIAFPNDFR RDAYFADLQK QYADKTKEEL EAAAIPVKVA GRIMLNRGSF IVLQDSSERL QVYVNRKTLP EETLAEIKTW DLGDIIGAEG VLARSGKGDL YVDMTSVRLL TKSLRPLPDK HHGLTDTEQR YRQRYVDLMV NEETRHTFRV RSQVIAHIRR FLSERGFLEV ETPMLQTIPG GAAAKPFETH HNALDMAMFL RIAPELYLKR LVVGGFEKVF EINRNFRNEG VSTRHNPEFT MLEFYQAYAD YEDNMDLTEE LFRELAQSVL GTTDVPYGDK VFHFGEPFVR LSVFDSILKY NPEITAADLN DVEKARAIAK KAGAKVLGHE GLGKLQVMIF EELVEHKLEQ PHFITRYPFE VSPLARRNDE DPSVTDRFEL FIGGREIANA YSELNDAEDQ AERFMLQVKE KDAGDDEAMH YDADFINALE YGMPPTAGEG IGIDRLVMLL TNSPSIRDVI LFPHMRPQA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG ACCAACAACT CGACCAGCAC GAACTGCAAC AGGAAGAAAA CAAGCTGATC GCCCAGCGCA AGGAAAAACT TGCCGCCGTG CGCGAAGCCC GGGCCATCGC CTTCCCCAAC GACTTCCGCC GCGACGCCTA CTTCGCGGAC CTGCAGAAAC AGTACGCCGA CAAGACCAAG GAAGAGCTGG AAGCCGCAGC GATCCCGGTC AAGGTGGCCG GTCGGATCAT GCTCAACCGC GGTTCCTTCA TCGTCCTCCA GGACAGCAGC GAGCGCCTGC AGGTCTACGT CAACCGCAAG ACCCTGCCGG AAGAAACCCT GGCCGAGATC AAGACCTGGG ACCTGGGCGA CATCATCGGC GCCGAAGGCG TGCTGGCCCG CTCGGGCAAG GGCGACCTGT ACGTCGACAT GACCAGCGTG CGCCTGCTGA CCAAGTCGCT GCGTCCGCTG CCGGACAAGC ACCATGGCCT GACCGACACC GAGCAGCGCT ACCGCCAGCG CTACGTCGAC CTGATGGTCA ACGAGGAAAC CCGCCACACC TTCCGCGTGC GTTCCCAGGT CATCGCCCAC ATCCGCCGTT TCCTCAGCGA GCGCGGTTTC CTCGAAGTGG AGACCCCGAT GCTGCAGACC ATCCCCGGCG GCGCCGCGGC CAAGCCGTTC GAGACCCATC ACAACGCGCT GGACATGGCC ATGTTCCTGC GCATCGCCCC GGAGCTGTAC CTCAAGCGCC TGGTGGTCGG CGGTTTCGAG AAGGTCTTCG AGATCAACCG CAACTTCCGT AACGAAGGCG TCTCGACCCG GCACAACCCC GAGTTCACCA TGCTCGAGTT CTACCAGGCC TACGCCGACT ACGAAGACAA CATGGACCTG ACCGAGGAAC TGTTCCGCGA ACTGGCCCAG TCGGTACTCG GTACCACCGA CGTGCCCTAC GGCGACAAGG TCTTCCACTT CGGCGAGCCG TTCGTGCGCC TGTCGGTGTT CGATTCGATC CTCAAGTACA ACCCGGAGAT CACCGCCGCC GACCTCAACG ACGTCGAGAA GGCGCGGGCC ATCGCCAAGA AGGCCGGCGC CAAGGTCCTC GGCCACGAAG GCCTGGGCAA GCTGCAGGTG ATGATTTTCG AGGAACTGGT GGAGCACAAG CTGGAGCAGC CGCACTTCAT CACCCGCTAC CCCTTCGAGG TTTCGCCGCT GGCCCGCCGC AACGACGAAG ACCCGAGCGT CACCGACCGC TTCGAGCTGT TCATCGGCGG CCGCGAGATC GCCAATGCCT ATTCCGAGCT GAACGACGCC GAGGACCAGG CCGAGCGCTT CATGTTGCAG GTCAAGGAGA AGGACGCCGG CGACGACGAG GCGATGCACT ACGACGCCGA CTTCATCAAT GCCCTGGAAT ACGGCATGCC GCCGACCGCC GGCGAAGGCA TCGGCATCGA CCGCCTGGTG ATGCTGCTGA CCAACTCGCC GTCGATCCGC GACGTGATCC TGTTCCCGCA CATGCGCCCG CAGGCCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for PsaeA.00612.a.B21.PD00477
| PURIFICATION DATe: | 1/22/2017 |
| CONCENTRATION: | 20mg/ml |
| OBSERVED MW: | 65kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM Tris pH 8.0, 200 mM NaCl, 1% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHEE NKLIAQRKEK LAAVREARAI AFPNDFRRDA YFADLQKQYA DKTKEELEAA AIPVKVAGRI MLNRGSFIVL QDSSERLQVY VNRKTLPEET LAEIKTWDLG DIIGAEGVLA RSGKGDLYVD MTSVRLLTKS LRPLPDKHHG LTDTEQRYRQ RYVDLMVNEE TRHTFRVRSQ VIAHIRRFLS ERGFLEVETP MLQTIPGGAA AKPFETHHNA LDMAMFLRIA PELYLKRLVV GGFEKVFEIN RNFRNEGVST RHNPEFTMLE FYQAYADYED NMDLTEELFR ELAQSVLGTT DVPYGDKVFH FGEPFVRLSV FDSILKYNPE ITAADLNDVE KARAIAKKAG AKVLGHEGLG KLQVMIFEEL VEHKLEQPHF ITRYPFEVSP LARRNDEDPS VTDRFELFIG GREIANAYSE LNDAEDQAER FMLQVKEKDA GDDEAMHYDA DFINALEYGM PPTAGEGIGI DRLVMLLTNS PSIRDVILFP HMRPQA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGAAGAAA ACAAGCTGAT CGCCCAGCGC AAGGAAAAAC TTGCCGCCGT GCGCGAAGCC CGGGCCATCG CCTTCCCCAA CGACTTCCGC CGCGACGCCT ACTTCGCGGA CCTGCAGAAA CAGTACGCCG ACAAGACCAA GGAAGAGCTG GAAGCCGCAG CGATCCCGGT CAAGGTGGCC GGTCGGATCA TGCTCAACCG CGGTTCCTTC ATCGTCCTCC AGGACAGCAG CGAGCGCCTG CAGGTCTACG TCAACCGCAA GACCCTGCCG GAAGAAACCC TGGCCGAGAT CAAGACCTGG GACCTGGGCG ACATCATCGG CGCCGAAGGC GTGCTGGCCC GCTCGGGCAA GGGCGACCTG TACGTCGACA TGACCAGCGT GCGCCTGCTG ACCAAGTCGC TGCGTCCGCT GCCGGACAAG CACCATGGCC TGACCGACAC CGAGCAGCGC TACCGCCAGC GCTACGTCGA CCTGATGGTC AACGAGGAAA CCCGCCACAC CTTCCGCGTG CGTTCCCAGG TCATCGCCCA CATCCGCCGT TTCCTCAGCG AGCGCGGTTT CCTCGAAGTG GAGACCCCGA TGCTGCAGAC CATCCCCGGC GGCGCCGCGG CCAAGCCGTT CGAGACCCAT CACAACGCGC TGGACATGGC CATGTTCCTG CGCATCGCCC CGGAGCTGTA CCTCAAGCGC CTGGTGGTCG GCGGTTTCGA GAAGGTCTTC GAGATCAACC GCAACTTCCG TAACGAAGGC GTCTCGACCC GGCACAACCC CGAGTTCACC ATGCTCGAGT TCTACCAGGC CTACGCCGAC TACGAAGACA ACATGGACCT GACCGAGGAA CTGTTCCGCG AACTGGCCCA GTCGGTACTC GGTACCACCG ACGTGCCCTA CGGCGACAAG GTCTTCCACT TCGGCGAGCC GTTCGTGCGC CTGTCGGTGT TCGATTCGAT CCTCAAGTAC AACCCGGAGA TCACCGCCGC CGACCTCAAC GACGTCGAGA AGGCGCGGGC CATCGCCAAG AAGGCCGGCG CCAAGGTCCT CGGCCACGAA GGCCTGGGCA AGCTGCAGGT GATGATTTTC GAGGAACTGG TGGAGCACAA GCTGGAGCAG CCGCACTTCA TCACCCGCTA CCCCTTCGAG GTTTCGCCGC TGGCCCGCCG CAACGACGAA GACCCGAGCG TCACCGACCG CTTCGAGCTG TTCATCGGCG GCCGCGAGAT CGCCAATGCC TATTCCGAGC TGAACGACGC CGAGGACCAG GCCGAGCGCT TCATGTTGCA GGTCAAGGAG AAGGACGCCG GCGACGACGA GGCGATGCAC TACGACGCCG ACTTCATCAA TGCCCTGGAA TACGGCATGC CGCCGACCGC CGGCGAAGGC ATCGGCATCG ACCGCCTGGT GATGCTGCTG ACCAACTCGC CGTCGATCCG CGACGTGATC CTGTTCCCGC ACATGCGCCC GCAGGCCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT