SSGCID: Target Page

PsaeA.00585.a

Lipoyl synthase (EC 2.8.1.8) (Lip-syn) (LS) (Lipoate synthase) (Lipoic acid synthase) (Sulfur insertion protein LipA)

CENTER ID:	PsaeA.00585.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.00585.a.B1.GE38242	full length		1	327

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.4188
PGAT:	pseudomonas:PA3996
RefSeq:	NP_252685.1
UniProt:	Q9HX25

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTVVEKSGE AKPGKVEVGV KLRGAEKVAR IPVKIIPTEE LPKKPDWIRV RIPVSPEVDR IKQLLRKHKL HSVCEEASCP NLGECFSGGT ATFMIMGDIC TRRCPFCDVG HGRPKPLDVD EPTNLAIAIA DLRLKYVVIT SVDRDDLRDG GAQHFADCLR EIRKLSPGIQ LETLVPDYRG RMDIALEITA NEPPDVFNHN LETVPRLYRS SRPGSDFEWS LDLLQKFKQM VPHVPTKSGL MLGLGETDDE VIEVMQRMRE HDIDMLTLGQ YLQPSRNHLP VQRFVHPDTF AWFAEEGEKM GFKNVASGPL VRSSYHADQQ AHGNKIG

NT Sequence

atgagcactg ttgtggagaa gtccggagaa gccaagccgg gcaaggtgga agtcggcgtg aagctgcgcg gcgcggaaaa ggtcgcgcgg attccggtga agatcattcc caccgaggaa ctgccgaaga aacccgactg gatccgcgtg cgcatcccgg tttcccccga ggtcgaccgc atcaagcaac tgctgcgcaa gcacaagctg cacagcgtct gcgaagaggc gtcctgcccg aacctgggcg agtgcttctc cggcggcacc gcgaccttca tgatcatggg cgatatctgc acccgacgct gcccgttctg cgacgtcggt catggccggc cgaagccgct ggacgtcgac gagccgacca acctggccat cgccatcgcc gacctgcggc tgaagtacgt ggtgatcacc tcggtggacc gcgacgacct gcgcgacggc ggcgcccagc acttcgccga ctgcctgcgc gagatccgca agctgtcgcc ggggatccag ctggaaaccc tggtgcccga ctatcgcggt cgcatggaca tcgccctgga gatcaccgcc aacgagccgc cggacgtgtt caaccataac ctggagacgg taccgcgcct gtaccgctcc tcgcggccgg gctcggactt cgagtggtcg ctggacctgc tgcagaagtt caagcagatg gttccgcacg taccgaccaa gtccgggctg atgctcggcc tgggcgagac cgacgacgag gtcatcgagg tcatgcagcg gatgcgcgag cacgacatcg acatgctgac cctcggccag tacctgcagc cgtcgcgcaa ccacctgccg gtgcagcgct tcgtgcatcc ggacaccttc gcctggttcg ccgaggaagg cgagaagatg ggcttcaaga acgtcgcgtc cggcccgctg gtgcgttcgt cctaccatgc cgaccagcag gcgcacggca acaagatcgg ctga

Details for PsaeA.00585.a.B1.GE38242

HARVESTED ON:	7/1/2014
SEQUENCED ON:	7/8/2014
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

atcttactca cttagccgat cttgttgccg tgcgcctgct ggtcggcatg gtaggacgaa cgcaccagcg ggccggacgc gacgttcttg aagcccatct tctcgccttc ctcggcgaac caggcgaagg tgtccggatg cacgaagcgc tgcaccggca ggtggttgcg cgacggctgc aggtactggc cgagggtcag catgtcgatg tcgtgctcgc gcatccgctg catgacctcg atgacctcgt cgtcggtctc gcccaggccg agcatcagcc cggacttggt cggtacgtgc ggaaccatct gcttgaactt ctgcagcagg tccagcgacc actcgaagtc cgagcccggc cgcgaggagc ggtacaggcg cggtaccgtc tccaggttat ggttgaacac gtccggcggc tcgttggcgg tgatctccag ggcgatgtcc atgcgaccgc gatagtcggg caccagggtt tccagctgga tccccggcga cagcttgcgg atctcgcgca ggcagtcggc gaagtgctgg gcgccgccgt cgcgcaggtc gtcgcggtcc accgaggtga tcaccacgta cttcagccgc aggtcggcga tggcgatggc caggttggtc ggctcgtcga cgtccagcgg cttcggccgg ccatgaccga cgtcgcagaa cgggcagcgt cgggtgcaga tatcgcccat gatcatgaag gtcgcggtgc cgccggagaa gcactcgccc aggttcgggc aggacgcctc ttcgcagacg ctgtgcagct tgtgcttgcg cagcagttgc ttgatgcggt cgacctcggg ggaaaccggg atgcgcacgc ggatccagtc gggtttcttc ggcagttcct cggtgggaat gatcttcacc ggaatccgcg cgaccttttc cgcgccgcgc agcttcacgc cgacttccac cttgcccggc ttggcttctc cggacttctc cacaacagtg ctcatatggt ggtggtggtg gtgagc

Expected Protein Sequence

MAHHHHHHMS TVVEKSGEAK PGKVEVGVKL RGAEKVARIP VKIIPTEELP KKPDWIRVRI PVSPEVDRIK QLLRKHKLHS VCEEASCPNL GECFSGGTAT FMIMGDICTR RCPFCDVGHG RPKPLDVDEP TNLAIAIADL RLKYVVITSV DRDDLRDGGA QHFADCLREI RKLSPGIQLE TLVPDYRGRM DIALEITANE PPDVFNHNLE TVPRLYRSSR PGSDFEWSLD LLQKFKQMVP HVPTKSGLML GLGETDDEVI EVMQRMREHD IDMLTLGQYL QPSRNHLPVQ RFVHPDTFAW FAEEGEKMGF KNVASGPLVR SSYHADQQAH GNKIG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca ctgttgtgga gaagtccgga gaagccaagc cgggcaaggt ggaagtcggc gtgaagctgc gcggcgcgga aaaggtcgcg cggattccgg tgaagatcat tcccaccgag gaactgccga agaaacccga ctggatccgc gtgcgcatcc cggtttcccc cgaggtcgac cgcatcaagc aactgctgcg caagcacaag ctgcacagcg tctgcgaaga ggcgtcctgc ccgaacctgg gcgagtgctt ctccggcggc accgcgacct tcatgatcat gggcgatatc tgcacccgac gctgcccgtt ctgcgacgtc ggtcatggcc ggccgaagcc gctggacgtc gacgagccga ccaacctggc catcgccatc gccgacctgc ggctgaagta cgtggtgatc acctcggtgg accgcgacga cctgcgcgac ggcggcgccc agcacttcgc cgactgcctg cgcgagatcc gcaagctgtc gccggggatc cagctggaaa ccctggtgcc cgactatcgc ggtcgcatgg acatcgccct ggagatcacc gccaacgagc cgccggacgt gttcaaccat aacctggaga cggtaccgcg cctgtaccgc tcctcgcggc cgggctcgga cttcgagtgg tcgctggacc tgctgcagaa gttcaagcag atggttccgc acgtaccgac caagtccggg ctgatgctcg gcctgggcga gaccgacgac gaggtcatcg aggtcatgca gcggatgcgc gagcacgaca tcgacatgct gaccctcggc cagtacctgc agccgtcgcg caaccacctg ccggtgcagc gcttcgtgca tccggacacc ttcgcctggt tcgccgagga aggcgagaag atgggcttca agaacgtcgc gtccggcccg ctggtgcgtt cgtcctacca tgccgaccag caggcgcacg gcaacaagat cggctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat