SSGCID: Target Page

PsaeA.00249.a

Thymidylate synthase (TS) (TSase) (EC 2.1.1.45)

CENTER ID:	PsaeA.00249.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.00249.a.B1.GE38234	full length		1	264

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|208964.12.peg.360
PGAT:	pseudomonas:PA0342
RefSeq:	NP_249033.1
UniProt:	Q9I6F1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKQYLDLMRH VREHGTFKSD RTGTGTYSVF GHQMRFDLAA GFPLVTTKKC HLKSIVHELL WFLKGSTNIA YLKEHGVSIW DEWADENGDL GPVYGYQWRS WPAPDGRHID QIANLMAMLK KNPDSRRLIV SAWNPALIDE MALPPCHALF QFYVADGKLS CQLYQRSADI FLGVPFNIAS YALLTLMVAQ VAGLQPGEFI WTGGDCHLYA NHLEQADLQL TREPLPLPSM KLNPEVKDLF DFRFEDFELV GYEAHPHIKA PVAV

NT Sequence

atgaaacagt acctcgacct gatgcgccac gtgcgcgagc acggcacctt caagagcgac cgcaccggca ccggcaccta tagcgtgttc ggccaccaga tgcgcttcga cctggccgcg ggcttccccc tggtgaccac caagaagtgc cacctcaagt cgatcgtcca cgagctgctg tggttcctca agggctccac caatatcgcc tacctcaagg agcacggcgt ctcgatctgg gacgaatggg ccgacgagaa cggcgacctc ggcccggtgt atggctacca gtggcgctcc tggccggcgc cggacggccg ccacatcgac cagatcgcca acctgatggc gatgctgaag aagaatccgg actcgcgccg gctgatcgtc tccgcctgga acccggcgct gatcgacgag atggccctgc cgccctgcca cgcgctgttc cagttctacg tcgccgacgg caagctcagc tgccagctct accaacgttc ggcggacatc ttcctcggcg tacccttcaa tatcgccagc tatgccctgc tgaccctgat ggtggcgcag gtcgccggcc tgcagccggg cgagttcatc tggaccggcg gcgattgcca cctgtacgcc aaccacctgg agcaggccga cctgcagctg acccgcgagc cgctgccgtt gccgagcatg aagctgaatc ccgaggtgaa ggatctgttc gactttcgct tcgaggactt cgagctggtc ggctacgagg cccatccgca catcaaggca ccggtcgcgg tctga

Details for PsaeA.00249.a.B1.GE38234

HARVESTED ON:	7/1/2014
SEQUENCED ON:	7/8/2014
EXPECTED MW:	31kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK QYLDLMRHVR EHGTFKSDRT GTGTYSVFGH QMRFDLAAGF PLVTTKKCHL KSIVHELLWF LKGSTNIAYL KEHGVSIWDE WADENGDLGP VYGYQWRSWP APDGRHIDQI ANLMAMLKKN PDSRRLIVSA WNPALIDEMA LPPCHALFQF YVADGKLSCQ LYQRSADIFL GVPFNIASYA LLTLMVAQVA GLQPGEFIWT GGDCHLYANH LEQADLQLTR EPLPLPSMKL NPEVKDLFDF RFEDFELVGY EAHPHIKAPV AV

Validated NT Sequence

atcttactca cttagaccgc gaccggtgcc ttgatgtgcg gatgggcctc gtagccgacc agctcgaagt cctcgaagcg aaagtcgaac agatccttca cctcgggatt cagcttcatg ctcggcaacg gcagcggctc gcgggtcagc tgcaggtcgg cctgctccag gtggttggcg tacaggtggc aatcgccgcc ggtccagatg aactcgcccg gctgcaggcc ggcgacctgc gccaccatca gggtcagcag ggcatagctg gcgatattga agggtacgcc gaggaagatg tccgccgaac gttggtagag ctggcagctg agcttgccgt cggcgacgta gaactggaac agcgcgtggc agggcggcag ggccatctcg tcgatcagcg ccgggttcca ggcggagacg atcagccggc gcgagtccgg attcttcttc agcatcgcca tcaggttggc gatctggtcg atgtggcggc cgtccggcgc cggccaggag cgccactggt agccatacac cgggccgagg tcgccgttct cgtcggccca ttcgtcccag atcgagacgc cgtgctcctt gaggtaggcg atattggtgg agcccttgag gaaccacagc agctcgtgga cgatcgactt gaggtggcac ttcttggtgg tcaccagggg gaagcccgcg gccaggtcga agcgcatctg gtggccgaac acgctatagg tgccggtgcc ggtgcggtcg ctcttgaagg tgccgtgctc gcgcacgtgg cgcatcaggt cgaggtactg tttcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac agtacctcga cctgatgcgc cacgtgcgcg agcacggcac cttcaagagc gaccgcaccg gcaccggcac ctatagcgtg ttcggccacc agatgcgctt cgacctggcc gcgggcttcc ccctggtgac caccaagaag tgccacctca agtcgatcgt ccacgagctg ctgtggttcc tcaagggctc caccaatatc gcctacctca aggagcacgg cgtctcgatc tgggacgaat gggccgacga gaacggcgac ctcggcccgg tgtatggcta ccagtggcgc tcctggccgg cgccggacgg ccgccacatc gaccagatcg ccaacctgat ggcgatgctg aagaagaatc cggactcgcg ccggctgatc gtctccgcct ggaacccggc gctgatcgac gagatggccc tgccgccctg ccacgcgctg ttccagttct acgtcgccga cggcaagctc agctgccagc tctaccaacg ttcggcggac atcttcctcg gcgtaccctt caatatcgcc agctatgccc tgctgaccct gatggtggcg caggtcgccg gcctgcagcc gggcgagttc atctggaccg gcggcgattg ccacctgtac gccaaccacc tggagcaggc cgacctgcag ctgacccgcg agccgctgcc gttgccgagc atgaagctga atcccgaggt gaaggatctg ttcgactttc gcttcgagga cttcgagctg gtcggctacg aggcccatcc gcacatcaag gcaccggtcg cggtctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat