SSGCID: Target Page

PsaeA.00206.a

Phosphopantothenoylcysteine synthase/(R)-4-prime-phospho-N-pantothenoylcysteine decarboxylase

CENTER ID:	PsaeA.00206.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.00206.a.B1.GE38232	full length		1	402

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.5575
PGAT:	pseudomonas:PA5320
RefSeq:	NP_254007.1
UniProt:	Q9HTN4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQRLYRKRIV LGVGGGIAAY KSAELVRRLR DQGAEVRVVM TQGGREFITP LTLQALSGHP VHTDLLDPAA EAAMGHIELA RWADLVLIAP ATADLMARLV QGVANDLLTT LVLATDAQIA LAPAMNQAMW RDTATQANAE LLRQRGFHLF GPAAGSQACG DVGLGRMLEA EELAQRAADC FQRQALTGVH VLITAGPTQE NIDPVRYITN HSSGKMGFAL AEAAVEAGAR VTLVTGPVHL PTPDRVQRVD VVSARDMLAA CEAAMPCDLL IASAAVADYR PEVVAAHKLK KDPTSGEGLL LQLVRNPDIL ATLAQREDRP FSVGFAAETE NLLDYAARKL KDKNLDLIVA NDVANPSIGF NSDENAITVI DRDLHPSTFA QTSKGKIARQ LVAFIADRLN QQ

NT Sequence

atgcagcggc tctatcggaa acgcatcgtc ctcggcgtgg gcggcggcat tgccgcctac aagagcgccg aactggtccg ccgcctgcgc gaccagggcg ctgaagtccg agtggtgatg acccagggcg gccgcgagtt catcacgccc ctgaccctcc aggcgttgtc cggccatccg gtgcataccg atctgctgga cccggcggcg gaagccgcga tgggccacat cgaactggcc cgctgggccg atctggtgct gatcgcaccg gctaccgccg acctcatggc gcgcctggtc cagggcgtgg ccaacgacct gctgaccacc ctggtgctgg ccaccgacgc gcaaatcgcc ctggcaccgg cgatgaacca ggccatgtgg cgcgacaccg cgacccaggc caacgccgag ctgctgcgcc agcgcggctt ccacctgttc ggtccggcag cgggcagcca ggcctgcggc gacgtcggcc tcggacgcat gctcgaagcc gaggaactgg cgcagcgcgc cgccgactgc ttccagcgcc aggcgctgac cggcgtgcat gtgctgatca ccgccggccc gacccaggaa aacatcgacc cggtgcgcta catcaccaac cacagttccg gaaagatggg cttcgccctc gccgaggcgg cggtggaagc cggcgcacgg gtgaccctgg tcaccggccc ggtgcacctg cccacgcccg accgggtgca gcgcgtcgac gtggtcagcg cccgcgacat gctggccgcc tgcgaggcgg cgatgccctg cgacctgctg atcgcctccg cggcggtcgc cgactaccgc ccggaagtgg tcgccgcgca caaactgaag aaagacccta ccagcggcga gggcttgctc ttgcagctgg tgcgcaatcc cgacattctc gccaccctcg cacagcgcga ggatcgtccc ttcagcgtgg gcttcgccgc agaaaccgaa aacctgctgg actatgctgc acgcaagctc aaggacaaga acctcgacct gatcgtcgcc aacgacgtgg ccaacccctc gatcggcttc aacagcgacg agaacgcgat cacggtgatc gaccgcgacc tgcatccgag caccttcgcc cagaccagca agggcaagat cgctcgccag ctggtcgcct tcatcgccga ccgcctcaac cagcaatga

Details for PsaeA.00206.a.B1.GE38232

HARVESTED ON:	7/1/2014
SEQUENCED ON:	3/3/2015
EXPECTED MW:	44kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMQ RLYRKRIVLG VGGGIAAYKS AELVRRLRDQ GAEVRVVMTQ GGREFITPLT LQALSGHPVH TDLLDPAAEA AMGHIELARW ADLVLIAPAT ADLMARLVQG VANDLLTTLV LATDAQIALA PAMNQAMWRD TATQANAELL RQRGFHLFGP AAGSQACGDV GLGRMLEAEE LAQRAADCFQ RQALTGVHVL ITAGPTQENI DPVRYITNHS SGKMGFALAE AAVEAGARVT LVTGPVHLPT PDRVQRVDVV SARDMLAACE AAMPCDLLIA SAAVADYRPE VVAAHKLKKD PTSGEGLLLQ LVRNPDILAT LAQREDRPFS VGFAAETENL LDYAARKLKD KNLDLIVAND VANPSIGFNS DENAITVIDR DLHPSTFAQT SKGKIARQLV A

Validated NT Sequence

atggctcacc accaccacca ccatatgcag cggctctatc ggaaacgcat cgtcctcggc gtgggcggcg gcattgccgc ctacaagagc gccgaactgg tccgccgcct gcgcgaccag ggcgctgaag tccgagtggt gatgacccag ggcggccgcg agttcatcac gcccctgacc ctccaggcgt tgtccggcca tccggtgcat accgatctgc tggacccggc ggcggaagcc gcgatgggcc acatcgaact ggcccgctgg gccgatctgg tgctgatcgc accggctacc gccgacctca tggcgcgcct ggtccagggc gtggccaacg acctgctgac caccctggtg ctggccaccg acgcgcaaat cgccctggca ccggcgatga accaggccat gtggcgcgac accgcgaccc aggccaacgc cgagctgctg cgccagcgcg gcttccacct gttcggtccg gcagcgggca gccaggcctg cggcgacgtc ggcctcggac gcatgctcga agccgaggaa ctggcgcagc gcgccgccga ctgcttccag cgccaggcgc tgaccggcgt gcatgtgctg atcaccgccg gcccgaccca ggaaaacatc gacccggtgc gctacatcac caaccacagt tccggaaaga tgggcttcgc cctcgccgag gcggcggtgg aagccggcgc acgggtgacc ctggtcaccg gcccggtgca cctgcccacg cccgaccggg tgcagcgcgt cgacgtggtc agcgcccgcg acatgctggc cgcctgcgag gcggcgatgc cctgcgacct gctgatcgcc tccgcggcgg tcgccgacta ccgcccggaa gtggtcgccg cgcacaaact gaagaaagac cctaccagcg gcgagggctt gctcttgcag ctggtgcgca atcccgacat tctcgccacc ctcgcacagc gcgaggatcg tcccttcagc gtgggcttcg ccgcagaaac cgaaaacctg ctggactatg ctgcacgcaa gctcaaggac aagaacctcg acctgatcgt cgccaacgac gtggccaacc cctcgatcgg cttcaacagc gacgagaacg cgatcacggt gatcgaccgc gacctgcatc cgagcacctt cgcccagacc agcaagggca agatcgctcg ccagctggtc gcctt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagc ggctctatcg gaaacgcatc gtcctcggcg tgggcggcgg cattgccgcc tacaagagcg ccgaactggt ccgccgcctg cgcgaccagg gcgctgaagt ccgagtggtg atgacccagg gcggccgcga gttcatcacg cccctgaccc tccaggcgtt gtccggccat ccggtgcata ccgatctgct ggacccggcg gcggaagccg cgatgggcca catcgaactg gcccgctggg ccgatctggt gctgatcgca ccggctaccg ccgacctcat ggcgcgcctg gtccagggcg tggccaacga cctgctgacc accctggtgc tggccaccga cgcgcaaatc gccctggcac cggcgatgaa ccaggccatg tggcgcgaca ccgcgaccca ggccaacgcc gagctgctgc gccagcgcgg cttccacctg ttcggtccgg cagcgggcag ccaggcctgc ggcgacgtcg gcctcggacg catgctcgaa gccgaggaac tggcgcagcg cgccgccgac tgcttccagc gccaggcgct gaccggcgtg catgtgctga tcaccgccgg cccgacccag gaaaacatcg acccggtgcg ctacatcacc aaccacagtt ccggaaagat gggcttcgcc ctcgccgagg cggcggtgga agccggcgca cgggtgaccc tggtcaccgg cccggtgcac ctgcccacgc ccgaccgggt gcagcgcgtc gacgtggtca gcgcccgcga catgctggcc gcctgcgagg cggcgatgcc ctgcgacctg ctgatcgcct ccgcggcggt cgccgactac cgcccggaag tggtcgccgc gcacaaactg aagaaagacc ctaccagcgg cgagggcttg ctcttgcagc tggtgcgcaa tcccgacatt ctcgccaccc tcgcacagcg cgaggatcgt cccttcagcg tgggcttcgc cgcagaaacc gaaaacctgc tggactatgc tgcacgcaag ctcaaggaca agaacctcga cctgatcgtc gccaacgacg tggccaaccc ctcgatcggc ttcaacagcg acgagaacgc gatcacggtg atcgaccgcg acctgcatcc gagcaccttc gcccagacca gcaagggcaa gatcgctcgc cagctggtcg ccttcatcgc cgaccgcctc aaccagcaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat