PsaeA.00177.b

Chaperone protein HscA homolog

CENTER ID: PsaeA.00177.b
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00177.b.B1.GE38230 full length 1 619
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00177.b.B1.PS02165 full length 1 619

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.3989
PGAT: pseudomonas:PA3810
RefSeq: NP_252499.1
UniProt: Q51382

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALLQIAEPG QSPKPHERRL AVGIDLGTTN SLVAAVRSGV AEPLPDAQGR LILPSAVRYH AERAEVGESA RAAAAEDPFN TVISVKRLMG RGLEDVKQLG EQLPYRFRQG ESHMPFIETV QGLKSPVEVS ADILRELRQR AETTLGGELV GAVITVPAYF DDAQRQATKD AARLAGLNVL RLLNEPTAAA VAYGLDKGAE GLVAIYDLGG GTFDISILRL TRGVFEVLAT GGDTALGGDD FDHAIAGWVI EQAGLSADLD PGSQRQLLQI ACAAKERLTD EASVRVAYGD WSGELSRATL DELIEPFVAR SLKSCRRAVR DSGVDLEEIR SVVMVGGSTR VPRVRTAVGE LFGCEPLTDI DPDQVVAIGA AIQADALAGN KRGEELLLLD VIPLSLGLET MGGLMEKVIP RNTTIPVARA QEFTTYKDGQ TAMMIHVLQG ERELVKDCRS LARFELRGIP PMVAGAAKIR VTFQVDADGL LGVSARELSS GVEASIQVKP SYGLTDGEIA RMLKDSFDYA GDDKAARALR EQQVEAQRLL EAVQSALDVD GERLLDEEER LAIAAQMDTL RELAGGSDTA AIENQIKRLS QVTDAFAARR MDATVKAALS GRRLNEIEE
NT Sequence
ATGGCCCTAC TGCAGATCGC CGAGCCCGGT CAGAGTCCCA AGCCCCATGA GCGTCGCCTG GCGGTTGGGA TCGACCTGGG TACCACCAAT TCGCTGGTCG CCGCGGTGCG TAGCGGCGTC GCCGAGCCCT TGCCGGATGC GCAGGGACGC CTGATCCTGC CTTCCGCGGT GCGTTATCAC GCCGAGCGGG CGGAAGTGGG CGAGAGCGCC CGCGCGGCCG CCGCGGAGGA TCCCTTCAAT ACCGTCATTT CGGTCAAGCG CCTGATGGGG CGCGGCCTGG AGGACGTCAA GCAACTGGGC GAGCAATTGC CCTACCGCTT CCGCCAGGGC GAGTCGCACA TGCCCTTCAT CGAGACCGTA CAGGGGTTGA AGAGTCCTGT GGAAGTCTCG GCGGACATCC TGCGTGAGCT GCGCCAGCGC GCCGAAACCA CCCTCGGTGG CGAGCTGGTG GGTGCGGTGA TCACTGTGCC GGCCTATTTC GACGACGCCC AGCGCCAGGC TACCAAGGAT GCCGCGCGCC TGGCCGGACT GAACGTCCTG CGCCTGCTCA ACGAGCCGAC CGCCGCCGCC GTGGCCTACG GCCTGGACAA GGGCGCGGAA GGCCTGGTGG CCATCTATGA CCTGGGTGGC GGAACCTTCG ACATTTCCAT CCTGCGCCTG ACCCGCGGTG TCTTCGAAGT CCTGGCCACC GGCGGCGATA CGGCCCTGGG TGGCGACGAC TTCGACCATG CCATCGCTGG CTGGGTGATC GAGCAGGCTG GCCTTTCTGC CGATCTGGAC CCGGGCAGCC AGCGGCAGTT GCTGCAAATC GCCTGTGCGG CCAAGGAGCG TCTTACCGAC GAGGCGTCGG TCCGGGTCGC CTATGGCGAC TGGAGCGGCG AGCTGAGCCG GGCGACGCTC GACGAGTTGA TCGAGCCGTT CGTCGCTCGC AGCCTGAAGT CCTGCCGCCG AGCGGTGCGC GACTCCGGTG TCGATCTGGA AGAAATCCGC TCGGTGGTGA TGGTCGGCGG CTCGACCCGC GTGCCCCGTG TGCGCACGGC GGTTGGCGAG TTGTTCGGCT GCGAGCCGCT GACCGATATC GATCCGGACC AGGTGGTCGC CATCGGCGCG GCCATCCAGG CCGATGCCCT GGCCGGCAAC AAGCGCGGCG AGGAATTGCT GCTGCTGGAC GTCATTCCGC TGTCGCTCGG CCTGGAAACC ATGGGCGGGC TGATGGAGAA GGTGATTCCG CGCAATACCA CCATCCCGGT GGCGCGCGCC CAGGAGTTCA CCACCTACAA GGACGGCCAG ACGGCCATGA TGATCCACGT GCTGCAGGGC GAGCGCGAGC TGGTGAAGGA TTGTCGTTCG CTGGCGCGCT TCGAGCTGCG CGGCATTCCG CCGATGGTGG CGGGCGCGGC GAAAATCCGG GTGACCTTCC AGGTCGACGC CGACGGCTTG CTCGGCGTGT CCGCACGCGA ACTGAGTTCC GGCGTCGAGG CCAGCATCCA GGTCAAGCCG TCCTATGGGC TGACCGACGG CGAGATCGCG CGGATGCTCA AGGACTCCTT CGACTACGCC GGCGACGACA AGGCTGCCCG CGCCCTGCGC GAACAGCAGG TCGAGGCGCA GCGGCTGCTG GAGGCCGTGC AGTCGGCGCT GGACGTCGAC GGCGAGCGGC TCCTGGACGA AGAGGAGCGC CTGGCCATCG CGGCGCAGAT GGACACTTTG CGTGAACTGG CCGGTGGTAG CGACACCGCG GCCATCGAGA ACCAGATCAA GCGCCTGTCC CAGGTGACCG ACGCTTTCGC CGCACGGCGG ATGGACGCGA CCGTCAAGGC CGCGCTCTCC GGCCGCCGGC TCAACGAAAT CGAGGAATAA
Details for PsaeA.00177.b.B1.GE38230
HARVESTED ON: 7/1/2014
SEQUENCED ON: 7/8/2014
EXPECTED MW: 67kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 49
PERCENT COVERAGE: 77
Validated AA Sequence
MGRGLEDVKQ LGEQLPYRFR QGESHMPFIE TVQGLKSPVE VSADILRELR QRAETTLGGE LVGAVITVPA YFDDAQRQAT KXAARLAGLN VLRLLNEPTA AAVAYGLDKG AEGLVAIYDL GGGTFDISIL RLTRGVFEVL ATGGDTALGG DDFDHAIAGW VIEXXAFLPI WTRAASGSCC KSPVRPXSVL PTXRRXXVAY GDWXGELXXX DARRVIEPFV ARXXXSCRRA XRXXXXRSGR XPLGGDGRRL DPRAPCAHGX WRVVRLRAAD RYRSGPGGRH RRXHPGRCXG RQQARRGIAX AGRHSAVARP GNHGRADGEG DSAQYHHPGG ARPGVHHLQG RPDGHDXPRA AGRARAGEGL SFAGALRAAR HSADGGGRGE NPGDLPGRRR RLARRVRTRT EFRRRGQHPG QAVLWADRRR DRADAQGLLR LRRRRQGCPR PARTAGRGAA AAGGRAVGAG RRRRAAPGRR GAPGHRGADG HFA
Validated NT Sequence
ggccggagag cgcggccttg acggtcgcgt ccatccgccg tgcggcgaaa gcgtcggtca cctgggacag gcgcttgatc tggttctcga tggccgcggt gtcgctacca ccggccagtt cacgcaaagt gtccatctgc gccgcgatgg ccaggcgctc ctcttcgtcc aggagccgct cgccgtcgac gtccagcgcc gactgcacgg cctccagcag ccgctgcgcc tcgacctgct gttcgcgcag ggcgcgggca gccttgtcgt cgccggcgta gtcgaaggag tccttgagca tccgcgcgat ctcgccgtcg gtcagcccat aggacggctt gacctggatg ctggcctcga cgccggaact cagttcgcgt gcggacacgc cgagcaagcc gtcggcgtcg acctggaagg tcacccggat tttcgccgcg cccgccacca tcggcggaat gccgcgcagc tcgaagcgcg ccagcgaacg acaatccttc accagctcgc gctcgccctg cagcacgtgg ntcatcatgg ccgtctggcc gtccttgtag gtggtgaact cctgggcgcg cgccaccggg atggtggtat tgcgcggaat caccttctcc atcagcccgc ccatggtttc caggccgagc gacagcggaa tgacgtccag cancagcaat tcctcgccgc gcttgttgcc ggccanggca tcggcctgga tggncgcgcc gatggcgacc acctggtccg gatcgatatc ggtcagcggc tcgcagccga acaactcgcc aancgccgtg cgcacacggg gcacgcgggt cgagccgccg accatcacca ccgagcggan ttcttccaga tcganaccng nantcgcgca ncgctcggcg gcaggacnnc angntgcgag cgacgaacgg ctcgatnact cgtcgagcgt cgnccngnct cagctcgccg nnccagtcgc cataggcgac ccngnaccga cgcntngtcg gtaagacgct nnttggccgc acaggcgatt tgcagcaact gccgctggct gcccgggtcc agatcggcag aaaggccann ngctcgatca cccagccagc gatggcatgg tcgaagtcgt cgccacccag ggccgtatcg ccgccggtgg ccaggacttc gaagacaccg cgggtcaggc gcaggatgga aatgtcgaag gttccgccac ccaggtcata gatggccacc aggccttccg cgcccttgtc caggccgtag gccacggcgg cggcggtcgg ctcgttgagc aggcgcagga cgttcagtcc ggccaggcgc gcggcancct tggtagcctg gcgctgggcg tcgtcgaaat aggccggcac agtgatcacc gcacccacca gctcgccacc gagggtggtt tcggcgcgct ggcgcagctc acgcaggatg tccgccgaga cttccacagg actcttcaac ccctgtacgg tctcgatgaa gggcatgtgc gactcgccct ggcggaagcg gtagggcaat tgctcgccca gttgcttgac gtcctccagg ccgcgcccca tcaggcgctt gaccgaaatg acggtattga agggatcctc cgcggcggcc gcgcgggcgc tctcgcccac ttccgcccgc tcggcgtgat aacgcaccgc ggaaggcagg atcaggcgtc cctgcgcatc cggcaagggc tcggcgacgc cgctacgcac cgcggcgacc agcgaattgg tggtacccag gtcgatccca accgccaggc gacgctcatg gggcttggga ctctgaccgg gctcggcgat ctgcagtagg
Expected Protein Sequence
MAHHHHHHMA LLQIAEPGQS PKPHERRLAV GIDLGTTNSL VAAVRSGVAE PLPDAQGRLI LPSAVRYHAE RAEVGESARA AAAEDPFNTV ISVKRLMGRG LEDVKQLGEQ LPYRFRQGES HMPFIETVQG LKSPVEVSAD ILRELRQRAE TTLGGELVGA VITVPAYFDD AQRQATKDAA RLAGLNVLRL LNEPTAAAVA YGLDKGAEGL VAIYDLGGGT FDISILRLTR GVFEVLATGG DTALGGDDFD HAIAGWVIEQ AGLSADLDPG SQRQLLQIAC AAKERLTDEA SVRVAYGDWS GELSRATLDE LIEPFVARSL KSCRRAVRDS GVDLEEIRSV VMVGGSTRVP RVRTAVGELF GCEPLTDIDP DQVVAIGAAI QADALAGNKR GEELLLLDVI PLSLGLETMG GLMEKVIPRN TTIPVARAQE FTTYKDGQTA MMIHVLQGER ELVKDCRSLA RFELRGIPPM VAGAAKIRVT FQVDADGLLG VSARELSSGV EASIQVKPSY GLTDGEIARM LKDSFDYAGD DKAARALREQ QVEAQRLLEA VQSALDVDGE RLLDEEERLA IAAQMDTLRE LAGGSDTAAI ENQIKRLSQV TDAFAARRMD ATVKAALSGR RLNEIEE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccc tactgcagat cgccgagccc ggtcagagtc ccaagcccca tgagcgtcgc ctggcggttg ggatcgacct gggtaccacc aattcgctgg tcgccgcggt gcgtagcggc gtcgccgagc ccttgccgga tgcgcaggga cgcctgatcc tgccttccgc ggtgcgttat cacgccgagc gggcggaagt gggcgagagc gcccgcgcgg ccgccgcgga ggatcccttc aataccgtca tttcggtcaa gcgcctgatg gggcgcggcc tggaggacgt caagcaactg ggcgagcaat tgccctaccg cttccgccag ggcgagtcgc acatgccctt catcgagacc gtacaggggt tgaagagtcc tgtggaagtc tcggcggaca tcctgcgtga gctgcgccag cgcgccgaaa ccaccctcgg tggcgagctg gtgggtgcgg tgatcactgt gccggcctat ttcgacgacg cccagcgcca ggctaccaag gatgccgcgc gcctggccgg actgaacgtc ctgcgcctgc tcaacgagcc gaccgccgcc gccgtggcct acggcctgga caagggcgcg gaaggcctgg tggccatcta tgacctgggt ggcggaacct tcgacatttc catcctgcgc ctgacccgcg gtgtcttcga agtcctggcc accggcggcg atacggccct gggtggcgac gacttcgacc atgccatcgc tggctgggtg atcgagcagg ctggcctttc tgccgatctg gacccgggca gccagcggca gttgctgcaa atcgcctgtg cggccaagga gcgtcttacc gacgaggcgt cggtccgggt cgcctatggc gactggagcg gcgagctgag ccgggcgacg ctcgacgagt tgatcgagcc gttcgtcgct cgcagcctga agtcctgccg ccgagcggtg cgcgactccg gtgtcgatct ggaagaaatc cgctcggtgg tgatggtcgg cggctcgacc cgcgtgcccc gtgtgcgcac ggcggttggc gagttgttcg gctgcgagcc gctgaccgat atcgatccgg accaggtggt cgccatcggc gcggccatcc aggccgatgc cctggccggc aacaagcgcg gcgaggaatt gctgctgctg gacgtcattc cgctgtcgct cggcctggaa accatgggcg ggctgatgga gaaggtgatt ccgcgcaata ccaccatccc ggtggcgcgc gcccaggagt tcaccaccta caaggacggc cagacggcca tgatgatcca cgtgctgcag ggcgagcgcg agctggtgaa ggattgtcgt tcgctggcgc gcttcgagct gcgcggcatt ccgccgatgg tggcgggcgc ggcgaaaatc cgggtgacct tccaggtcga cgccgacggc ttgctcggcg tgtccgcacg cgaactgagt tccggcgtcg aggccagcat ccaggtcaag ccgtcctatg ggctgaccga cggcgagatc gcgcggatgc tcaaggactc cttcgactac gccggcgacg acaaggctgc ccgcgccctg cgcgaacagc aggtcgaggc gcagcggctg ctggaggccg tgcagtcggc gctggacgtc gacggcgagc ggctcctgga cgaagaggag cgcctggcca tcgcggcgca gatggacact ttgcgtgaac tggccggtgg tagcgacacc gcggccatcg agaaccagat caagcgcctg tcccaggtga ccgacgcttt cgccgcacgg cggatggacg cgaccgtcaa ggccgcgctc tccggccgcc ggctcaacga aatcgaggaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for PsaeA.00177.b.B1.PS02165
PURIFICATION DATe: 9/3/2014
CONCENTRATION: 30.1mg/ml
OBSERVED MW: 70kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 49
PERCENT COVERAGE: 77
Protocol Notes
notes unavailable
Validated AA Sequence
MGRGLEDVKQ LGEQLPYRFR QGESHMPFIE TVQGLKSPVE VSADILRELR QRAETTLGGE LVGAVITVPA YFDDAQRQAT KXAARLAGLN VLRLLNEPTA AAVAYGLDKG AEGLVAIYDL GGGTFDISIL RLTRGVFEVL ATGGDTALGG DDFDHAIAGW VIEXXAFLPI WTRAASGSCC KSPVRPXSVL PTXRRXXVAY GDWXGELXXX DARRVIEPFV ARXXXSCRRA XRXXXXRSGR XPLGGDGRRL DPRAPCAHGX WRVVRLRAAD RYRSGPGGRH RRXHPGRCXG RQQARRGIAX AGRHSAVARP GNHGRADGEG DSAQYHHPGG ARPGVHHLQG RPDGHDXPRA AGRARAGEGL SFAGALRAAR HSADGGGRGE NPGDLPGRRR RLARRVRTRT EFRRRGQHPG QAVLWADRRR DRADAQGLLR LRRRRQGCPR PARTAGRGAA AAGGRAVGAG RRRRAAPGRR GAPGHRGADG HFA
Validated NT Sequence
ggccggagag cgcggccttg acggtcgcgt ccatccgccg tgcggcgaaa gcgtcggtca cctgggacag gcgcttgatc tggttctcga tggccgcggt gtcgctacca ccggccagtt cacgcaaagt gtccatctgc gccgcgatgg ccaggcgctc ctcttcgtcc aggagccgct cgccgtcgac gtccagcgcc gactgcacgg cctccagcag ccgctgcgcc tcgacctgct gttcgcgcag ggcgcgggca gccttgtcgt cgccggcgta gtcgaaggag tccttgagca tccgcgcgat ctcgccgtcg gtcagcccat aggacggctt gacctggatg ctggcctcga cgccggaact cagttcgcgt gcggacacgc cgagcaagcc gtcggcgtcg acctggaagg tcacccggat tttcgccgcg cccgccacca tcggcggaat gccgcgcagc tcgaagcgcg ccagcgaacg acaatccttc accagctcgc gctcgccctg cagcacgtgg ntcatcatgg ccgtctggcc gtccttgtag gtggtgaact cctgggcgcg cgccaccggg atggtggtat tgcgcggaat caccttctcc atcagcccgc ccatggtttc caggccgagc gacagcggaa tgacgtccag cancagcaat tcctcgccgc gcttgttgcc ggccanggca tcggcctgga tggncgcgcc gatggcgacc acctggtccg gatcgatatc ggtcagcggc tcgcagccga acaactcgcc aancgccgtg cgcacacggg gcacgcgggt cgagccgccg accatcacca ccgagcggan ttcttccaga tcganaccng nantcgcgca ncgctcggcg gcaggacnnc angntgcgag cgacgaacgg ctcgatnact cgtcgagcgt cgnccngnct cagctcgccg nnccagtcgc cataggcgac ccngnaccga cgcntngtcg gtaagacgct nnttggccgc acaggcgatt tgcagcaact gccgctggct gcccgggtcc agatcggcag aaaggccann ngctcgatca cccagccagc gatggcatgg tcgaagtcgt cgccacccag ggccgtatcg ccgccggtgg ccaggacttc gaagacaccg cgggtcaggc gcaggatgga aatgtcgaag gttccgccac ccaggtcata gatggccacc aggccttccg cgcccttgtc caggccgtag gccacggcgg cggcggtcgg ctcgttgagc aggcgcagga cgttcagtcc ggccaggcgc gcggcancct tggtagcctg gcgctgggcg tcgtcgaaat aggccggcac agtgatcacc gcacccacca gctcgccacc gagggtggtt tcggcgcgct ggcgcagctc acgcaggatg tccgccgaga cttccacagg actcttcaac ccctgtacgg tctcgatgaa gggcatgtgc gactcgccct ggcggaagcg gtagggcaat tgctcgccca gttgcttgac gtcctccagg ccgcgcccca tcaggcgctt gaccgaaatg acggtattga agggatcctc cgcggcggcc gcgcgggcgc tctcgcccac ttccgcccgc tcggcgtgat aacgcaccgc ggaaggcagg atcaggcgtc cctgcgcatc cggcaagggc tcggcgacgc cgctacgcac cgcggcgacc agcgaattgg tggtacccag gtcgatccca accgccaggc gacgctcatg gggcttggga ctctgaccgg gctcggcgat ctgcagtagg
Expressed Protein Sequence
MAHHHHHHMA LLQIAEPGQS PKPHERRLAV GIDLGTTNSL VAAVRSGVAE PLPDAQGRLI LPSAVRYHAE RAEVGESARA AAAEDPFNTV ISVKRLMGRG LEDVKQLGEQ LPYRFRQGES HMPFIETVQG LKSPVEVSAD ILRELRQRAE TTLGGELVGA VITVPAYFDD AQRQATKDAA RLAGLNVLRL LNEPTAAAVA YGLDKGAEGL VAIYDLGGGT FDISILRLTR GVFEVLATGG DTALGGDDFD HAIAGWVIEQ AGLSADLDPG SQRQLLQIAC AAKERLTDEA SVRVAYGDWS GELSRATLDE LIEPFVARSL KSCRRAVRDS GVDLEEIRSV VMVGGSTRVP RVRTAVGELF GCEPLTDIDP DQVVAIGAAI QADALAGNKR GEELLLLDVI PLSLGLETMG GLMEKVIPRN TTIPVARAQE FTTYKDGQTA MMIHVLQGER ELVKDCRSLA RFELRGIPPM VAGAAKIRVT FQVDADGLLG VSARELSSGV EASIQVKPSY GLTDGEIARM LKDSFDYAGD DKAARALREQ QVEAQRLLEA VQSALDVDGE RLLDEEERLA IAAQMDTLRE LAGGSDTAAI ENQIKRLSQV TDAFAARRMD ATVKAALSGR RLNEIEE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCC TACTGCAGAT CGCCGAGCCC GGTCAGAGTC CCAAGCCCCA TGAGCGTCGC CTGGCGGTTG GGATCGACCT GGGTACCACC AATTCGCTGG TCGCCGCGGT GCGTAGCGGC GTCGCCGAGC CCTTGCCGGA TGCGCAGGGA CGCCTGATCC TGCCTTCCGC GGTGCGTTAT CACGCCGAGC GGGCGGAAGT GGGCGAGAGC GCCCGCGCGG CCGCCGCGGA GGATCCCTTC AATACCGTCA TTTCGGTCAA GCGCCTGATG GGGCGCGGCC TGGAGGACGT CAAGCAACTG GGCGAGCAAT TGCCCTACCG CTTCCGCCAG GGCGAGTCGC ACATGCCCTT CATCGAGACC GTACAGGGGT TGAAGAGTCC TGTGGAAGTC TCGGCGGACA TCCTGCGTGA GCTGCGCCAG CGCGCCGAAA CCACCCTCGG TGGCGAGCTG GTGGGTGCGG TGATCACTGT GCCGGCCTAT TTCGACGACG CCCAGCGCCA GGCTACCAAG GATGCCGCGC GCCTGGCCGG ACTGAACGTC CTGCGCCTGC TCAACGAGCC GACCGCCGCC GCCGTGGCCT ACGGCCTGGA CAAGGGCGCG GAAGGCCTGG TGGCCATCTA TGACCTGGGT GGCGGAACCT TCGACATTTC CATCCTGCGC CTGACCCGCG GTGTCTTCGA AGTCCTGGCC ACCGGCGGCG ATACGGCCCT GGGTGGCGAC GACTTCGACC ATGCCATCGC TGGCTGGGTG ATCGAGCAGG CTGGCCTTTC TGCCGATCTG GACCCGGGCA GCCAGCGGCA GTTGCTGCAA ATCGCCTGTG CGGCCAAGGA GCGTCTTACC GACGAGGCGT CGGTCCGGGT CGCCTATGGC GACTGGAGCG GCGAGCTGAG CCGGGCGACG CTCGACGAGT TGATCGAGCC GTTCGTCGCT CGCAGCCTGA AGTCCTGCCG CCGAGCGGTG CGCGACTCCG GTGTCGATCT GGAAGAAATC CGCTCGGTGG TGATGGTCGG CGGCTCGACC CGCGTGCCCC GTGTGCGCAC GGCGGTTGGC GAGTTGTTCG GCTGCGAGCC GCTGACCGAT ATCGATCCGG ACCAGGTGGT CGCCATCGGC GCGGCCATCC AGGCCGATGC CCTGGCCGGC AACAAGCGCG GCGAGGAATT GCTGCTGCTG GACGTCATTC CGCTGTCGCT CGGCCTGGAA ACCATGGGCG GGCTGATGGA GAAGGTGATT CCGCGCAATA CCACCATCCC GGTGGCGCGC GCCCAGGAGT TCACCACCTA CAAGGACGGC CAGACGGCCA TGATGATCCA CGTGCTGCAG GGCGAGCGCG AGCTGGTGAA GGATTGTCGT TCGCTGGCGC GCTTCGAGCT GCGCGGCATT CCGCCGATGG TGGCGGGCGC GGCGAAAATC CGGGTGACCT TCCAGGTCGA CGCCGACGGC TTGCTCGGCG TGTCCGCACG CGAACTGAGT TCCGGCGTCG AGGCCAGCAT CCAGGTCAAG CCGTCCTATG GGCTGACCGA CGGCGAGATC GCGCGGATGC TCAAGGACTC CTTCGACTAC GCCGGCGACG ACAAGGCTGC CCGCGCCCTG CGCGAACAGC AGGTCGAGGC GCAGCGGCTG CTGGAGGCCG TGCAGTCGGC GCTGGACGTC GACGGCGAGC GGCTCCTGGA CGAAGAGGAG CGCCTGGCCA TCGCGGCGCA GATGGACACT TTGCGTGAAC TGGCCGGTGG TAGCGACACC GCGGCCATCG AGAACCAGAT CAAGCGCCTG TCCCAGGTGA CCGACGCTTT CGCCGCACGG CGGATGGACG CGACCGTCAA GGCCGCGCTC TCCGGCCGCC GGCTCAACGA AATCGAGGAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT