PsaeA.00177.a

Chaperone protein DnaK (HSP70) (Heat shock 70 kDa protein) (Heat shock protein 70)

CENTER ID: PsaeA.00177.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00177.a.B1.GE38229 full length 1 637
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|208964.12.peg.4987
PGAT: pseudomonas:PA4761
RefSeq: NP_253449.1
UniProt: Q9HV43

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKIIGIDLG TTNSCVAILE NGNVKVIENA EGARTTPSII AYTNDGETLV GQPAKRQAVT NPQNTLYAVK RLIGRRFEEN VVQKDIQMVP YSIVKADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDSQ RQATKDAGRI AGLDVKRIIN EPTAAALAYG LDKAKGDHTV IVYDLGGGTF DVSVIEIAEV DGEHQFEVLA TNGDTFLGGE DFDIRLIDYL VDEFKKESGI NLKGDPLAMQ RLKEAAEKAK IELSSTQQTD VNLPYVTADA SGPKHLNVKV SRAKLESLVE DLVQRTIEPC RTALKDAGLD VSDIHEVILV GGQTRMPLVQ KTVAEFFGKE ARKDVNPDEA VAVGAAIQGA VLAGDVKDVL LLDVTPLTLG IETLGGVMTG LIEKNTTIPT KKSQVFSTAD DNQGAVTIHV LQGERKQAAQ NKSLGKFDLA DIPPAPRGVP QIEVTFDIDA NGILHVSAKD KATGKQQSIV IKASSGLSED EIQQMVRDAE ANAEEDRKFE ELAAARNQGD ALVHATRKMI TEAGDKATAE DKATIEKALG ELEAAVKGDD KAEIEAKMNA LSQASTPLAQ KMYAEQAQQG EDAPQGEQAK AADDVVDAEF EEVKDNK
NT Sequence
atgggcaaaa tcattggcat cgacctgggt accaccaact cctgcgtggc tatcctggag aacggtaacg tcaaggtcat cgagaacgcc gagggcgcgc gtaccacccc ctcgatcatc gcctacacca acgatggcga aaccctggtg ggccagccgg ccaagcgcca ggcggtcacc aacccgcaga acaccctgta tgcggtgaag cgcctgatcg gccgtcgctt cgaagagaac gtggttcaga aagacatcca gatggtgccg tacagcatcg tcaaggccga caacggcgac gcctgggtgg aagtgaaggg ccagaagatg gcgcctccgc agatttccgc cgaagtgctg aagaaaatga agaagaccgc cgaagactac ctcggcgagc cggtcaccga agcggtgatc accgttccgg cctacttcaa cgacagccag cgccaggcca ccaaggacgc cggccgcatc gccggcctcg acgtcaagcg gatcatcaac gagccgaccg cggcggcgct ggcctacggc ctggacaagg ccaagggcga ccacaccgtg atcgtctatg acctgggcgg cggtaccttc gacgtgtcgg tgatcgagat cgccgaagtc gacggcgagc accagttcga agtgctggcc accaacggcg acaccttcct cggcggcgaa gacttcgaca tccgcctgat cgactacctc gtcgacgagt tcaagaagga aagcggcatc aacctgaagg gcgacccgct ggccatgcag cgtctgaagg aagcggccga gaaagccaag atcgagctgt cctcgaccca gcagaccgac gtcaacctgc cgtacgtgac tgccgacgcg agcggtccga agcacctgaa cgtcaaggtt tcccgcgcca agctggagtc cctggtcgaa gacctggtgc agcgcaccat cgagccgtgc cgcaccgcgc tgaaggatgc cggcctggat gtttccgata tccatgaagt gatcctggtc ggcggccaga cccgcatgcc gctggtacag aagaccgttg ccgagttctt cggcaaggaa gcgcgcaagg acgtcaaccc ggacgaagcc gtggctgtcg gtgccgccat ccagggcgcg gtcctggccg gcgacgtgaa ggacgtgctg ctgctcgacg tgaccccgct gaccctcggc atcgagaccc tgggcggcgt gatgaccggt ctgatcgaga agaacaccac catcccgacc aagaagtcgc aggtgttctc caccgccgac gacaaccagg gcgcggtgac catccacgtg ctgcagggcg agcgcaagca ggccgcgcag aacaagtcgc tgggcaagtt cgacctggcc gacattccgc cggctccgcg cggcgtgccg cagatcgagg tgaccttcga tatcgatgcc aacggcatcc tgcacgtctc ggcgaaggac aaggccaccg gcaagcagca gtccatcgtg atcaaggcat cctccggcct gtccgaggat gagatccagc agatggtccg cgatgccgag gcgaacgccg aggaggaccg caagttcgag gaactggctg ccgctcgcaa ccagggcgac gcgctggtcc acgcgacccg caagatgatc accgaggcgg gcgacaaggc caccgccgag gacaaggcga ccatcgagaa ggcgctgggc gagctggaag cggcggtgaa gggcgacgac aaggccgaga tcgaggccaa gatgaacgct ctgtcccagg cttccacccc gctggcgcag aagatgtacg ccgaacaggc ccagcagggc gaagacgctc cccagggcga gcaggcgaaa gccgctgacg acgtggtgga cgccgagttc gaagaggtca aggacaacaa gtaa
Details for PsaeA.00177.a.B1.GE38229
HARVESTED ON: 7/1/2014
SEQUENCED ON: 7/8/2014
EXPECTED MW: 69kDa
OBSERVED MW: 75kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 17
PERCENT COVERAGE: 87
Validated AA Sequence
MVALSSAVAL SPASVIILRV AWTSASPWLR AAASSSNLRS SSAFASASRT ICWISSSDRP EDALITMDCC LPVALSFAET CRMPLASISK VTSICGTPRG AGGMSARSNL PSDLFCAACL RSPCSTWMVT APWLSSAVEN TCDFLVGMVV FFSIRPVITP PRVSMPXVSG VTSSSSTSFT SPAXTAPXMA APTATASXGL TSLRAXXPXX SXTVFCTXGM XVWPXTXITX XXSEXSXPXI XXRGAXXXDG XLXXXXPGXX SXXXXXXTFR CFGPLASXVT YGRXTSVCWV EDSSIXAXSA ASFRRCMASG SPXQVDAAFL LELVDEVVDQ ADVEVFAAEE GVAVGGQHFE LVLAVDFGDL DHRHVEGTAA QVIDDHGVVA LGLVQAVGQR RRGRLVDDPL DVEAGDAAGV LGGLALAVVE VGRNGDHRFG DRLAEVVFGG LLHFLQHFGG NLRRRHLLAL HFHPGVAVVG LDDAVRHHLD VFLNHVLFEA TADQALHRIQ GVLRVGDRLA LGRLAHQGFA IVGVGDDRGG GTRALGVLDD LDVTVLQDSH AGVGGTQVDA ND
Validated NT Sequence
cggcgtccac cacgtcgtca gcggctttcg cctgctcgcc ctggggagcg tcttcgccct gctgggcctg ttcggcgtac atcttctgcg ccagcggggt ggaagcctgg gacagagcgt tcatcttggc ctcgatctcg gccttgtcgt cgcccttcac cgccgcttcc agctcgccca gcgccttctc gatggtcgcc ttgtcctcgg cggtggcctt gtcgcccgcc tcggtgatca tcttgcgggt cgcgtggacc agcgcgtcgc cctggttgcg agcggcagcc agttcctcga acttgcggtc ctcctcggcg ttcgcctcgg catcgcggac catctgctgg atctcatcct cggacaggcc ggaggatgcc ttgatcacga tggactgctg cttgccggtg gccttgtcct tcgccgagac gtgcaggatg ccgttggcat cgatatcgaa ggtcacctcg atctgcggca cgccgcgcgg agccggcgga atgtcggcca ggtcgaactt gcccagcgac ttgttctgcg cggcctgctt gcgctcgccc tgcagcacgt ggatggtcac cgcgccctgg ttgtcgtcgg cggtggagaa cacctgcgac ttcttggtcg ggatggtggt gttcttctcg atcagaccgg tcatcacgcc gcccagggtc tcgatgccga nggtcagcgg ggtcacgtcn agcagcagca cgtccttcac gtcgccngcc angaccgcgc ccnngatggc ggcaccgaca gccacngctt cnnncgggtt gacgtccttg cgcgcttncn tgccgaanaa ntcnncaacg gtcttctgta ccancggcat gcnggtctgg ccgnnnaccn ngatcacttn annnanntcg gaaanntccn ngccngnnat cnnnnnncgn ggngcnnnnn nncncgatgg ngnnctgnnc nnnnnnnnac cngggantnc nagcnnnncg nggnaanncn ngacgttcag gtgcttcgga ccgctcgcgt cgncagtcac gtacggcagg tngacgtcgg tctgctgggt cgaggacagc tcgatctngg ctttntcggc cgcttccttc agacgctgca tggccagcgg gtcgcccntn caggtngatg ccgctttcct tcttgaactc gtcgacgagg tagtcgatca ggcggatgtc gaagtcttcg ccgccgagga aggtgtcgcc gttggtggcc agcacttcga actggtgctc gccgtcgact tcggcgatct cgatcaccga cacgtcgaag gtaccgccgc ccaggtcata gacgatcacg gtgtggtcgc ccttggcctt gtccaggccg taggccagcg ccgccgcggt cggctcgttg atgatccgct tgacgtcgag gccggcgatg cggccggcgt ccttggtggc ctggcgctgg ctgtcgttga agtaggccgg aacggtgatc accgcttcgg tgaccggctc gccgaggtag tcttcggcgg tcttcttcat tttcttcagc acttcggcgg aaatctgcgg aggcgccatc ttctggccct tcacttccac ccaggcgtcg ccgttgtcgg ccttgacgat gctgtacggc accatctgga tgtctttctg aaccacgttc tcttcgaagc gacggccgat caggcgcttc accgcataca gggtgttctg cgggttggtg accgcctggc gcttggccgg ctggcccacc agggtttcgc catcgttggt gtaggcgatg atcgaggggg tggtacgcgc gccctcggcg ttctcgatga ccttgacgtt accgttctcc aggatagcca cgcaggagtt ggtggtaccc aggtcgatgc caatgattt
Expected Protein Sequence
MAHHHHHHMG KIIGIDLGTT NSCVAILENG NVKVIENAEG ARTTPSIIAY TNDGETLVGQ PAKRQAVTNP QNTLYAVKRL IGRRFEENVV QKDIQMVPYS IVKADNGDAW VEVKGQKMAP PQISAEVLKK MKKTAEDYLG EPVTEAVITV PAYFNDSQRQ ATKDAGRIAG LDVKRIINEP TAAALAYGLD KAKGDHTVIV YDLGGGTFDV SVIEIAEVDG EHQFEVLATN GDTFLGGEDF DIRLIDYLVD EFKKESGINL KGDPLAMQRL KEAAEKAKIE LSSTQQTDVN LPYVTADASG PKHLNVKVSR AKLESLVEDL VQRTIEPCRT ALKDAGLDVS DIHEVILVGG QTRMPLVQKT VAEFFGKEAR KDVNPDEAVA VGAAIQGAVL AGDVKDVLLL DVTPLTLGIE TLGGVMTGLI EKNTTIPTKK SQVFSTADDN QGAVTIHVLQ GERKQAAQNK SLGKFDLADI PPAPRGVPQI EVTFDIDANG ILHVSAKDKA TGKQQSIVIK ASSGLSEDEI QQMVRDAEAN AEEDRKFEEL AAARNQGDAL VHATRKMITE AGDKATAEDK ATIEKALGEL EAAVKGDDKA EIEAKMNALS QASTPLAQKM YAEQAQQGED APQGEQAKAA DDVVDAEFEE VKDNK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggca aaatcattgg catcgacctg ggtaccacca actcctgcgt ggctatcctg gagaacggta acgtcaaggt catcgagaac gccgagggcg cgcgtaccac cccctcgatc atcgcctaca ccaacgatgg cgaaaccctg gtgggccagc cggccaagcg ccaggcggtc accaacccgc agaacaccct gtatgcggtg aagcgcctga tcggccgtcg cttcgaagag aacgtggttc agaaagacat ccagatggtg ccgtacagca tcgtcaaggc cgacaacggc gacgcctggg tggaagtgaa gggccagaag atggcgcctc cgcagatttc cgccgaagtg ctgaagaaaa tgaagaagac cgccgaagac tacctcggcg agccggtcac cgaagcggtg atcaccgttc cggcctactt caacgacagc cagcgccagg ccaccaagga cgccggccgc atcgccggcc tcgacgtcaa gcggatcatc aacgagccga ccgcggcggc gctggcctac ggcctggaca aggccaaggg cgaccacacc gtgatcgtct atgacctggg cggcggtacc ttcgacgtgt cggtgatcga gatcgccgaa gtcgacggcg agcaccagtt cgaagtgctg gccaccaacg gcgacacctt cctcggcggc gaagacttcg acatccgcct gatcgactac ctcgtcgacg agttcaagaa ggaaagcggc atcaacctga agggcgaccc gctggccatg cagcgtctga aggaagcggc cgagaaagcc aagatcgagc tgtcctcgac ccagcagacc gacgtcaacc tgccgtacgt gactgccgac gcgagcggtc cgaagcacct gaacgtcaag gtttcccgcg ccaagctgga gtccctggtc gaagacctgg tgcagcgcac catcgagccg tgccgcaccg cgctgaagga tgccggcctg gatgtttccg atatccatga agtgatcctg gtcggcggcc agacccgcat gccgctggta cagaagaccg ttgccgagtt cttcggcaag gaagcgcgca aggacgtcaa cccggacgaa gccgtggctg tcggtgccgc catccagggc gcggtcctgg ccggcgacgt gaaggacgtg ctgctgctcg acgtgacccc gctgaccctc ggcatcgaga ccctgggcgg cgtgatgacc ggtctgatcg agaagaacac caccatcccg accaagaagt cgcaggtgtt ctccaccgcc gacgacaacc agggcgcggt gaccatccac gtgctgcagg gcgagcgcaa gcaggccgcg cagaacaagt cgctgggcaa gttcgacctg gccgacattc cgccggctcc gcgcggcgtg ccgcagatcg aggtgacctt cgatatcgat gccaacggca tcctgcacgt ctcggcgaag gacaaggcca ccggcaagca gcagtccatc gtgatcaagg catcctccgg cctgtccgag gatgagatcc agcagatggt ccgcgatgcc gaggcgaacg ccgaggagga ccgcaagttc gaggaactgg ctgccgctcg caaccagggc gacgcgctgg tccacgcgac ccgcaagatg atcaccgagg cgggcgacaa ggccaccgcc gaggacaagg cgaccatcga gaaggcgctg ggcgagctgg aagcggcggt gaagggcgac gacaaggccg agatcgaggc caagatgaac gctctgtccc aggcttccac cccgctggcg cagaagatgt acgccgaaca ggcccagcag ggcgaagacg ctccccaggg cgagcaggcg aaagccgctg acgacgtggt ggacgccgag ttcgaagagg tcaaggacaa caagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat