PsaeA.00168.a

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60) (4-diphosphocytidyl-2C-methyl-D-erythritol synthase) (MEP cytidylyltransferase) (MCT)

CENTER ID: PsaeA.00168.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00168.a.B1.GE38227 full length 1 234
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.3802
PGAT: pseudomonas:PA3633
RefSeq: NP_252323.1
UniProt: P57707

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTSDLPAFW TVIPAAGVGS RMRADRPKQY LDLAGRTVIE RTLDCFLEHP MLRGLVVCLA EDDPYWPGLD CAASRHVQRA AGGAERAGSV LNGLLRLLEL GAQADDWVLV HDAARPNLTR GDLDRLLEEL AEDPVGGLLA VPARDTLKRS DRDGRVSETI DRSVVWLAYT PQMFRLGALH RALADALVAG VAITDEASAM EWAGYAPKLV EGRADNLKIT TPEDLLRLQR SFPH
NT Sequence
atgacgactt ccgaccttcc cgccttctgg acggtgatcc ccgccgccgg cgtgggttcg cgcatgcgcg ccgaccgccc gaagcaatat ctcgatcttg ccggtcgcac cgtgatcgag cgtaccctcg attgtttcct cgaacacccg atgctcaggg gcctggtggt ctgcctggcc gaggacgatc cctactggcc cgggctggac tgcgccgcca gccggcatgt gcagcgcgcc gccgggggcg ccgagcgcgc cggctcggtg ttgaacggct tgctgcgcct gctggagttg ggggcacagg ccgacgactg ggtgctggtg catgacgccg cgcggccgaa cctgacccgc ggcgacctcg accgcctgct cgaagaactc gccgaagatc cggtcggcgg gctgctggcg gtgccggcga gggacactct caagcgctcc gaccgcgacg gccgggtcag cgaaaccatc gaccgctcgg tggtctggct ggcctatacg ccgcagatgt tccgcctcgg cgccctgcac cgggcgctgg ccgatgcgtt ggtggccggc gtggcgatca ccgacgaggc ttcggcgatg gagtgggccg gctatgcgcc gaagctggtg gaagggcgcg ccgacaacct gaagatcacc accccggaag acctgctgcg cctgcagcgc agttttccgc actga
Details for PsaeA.00168.a.B1.GE38227
HARVESTED ON: 7/1/2014
SEQUENCED ON: 7/8/2014
EXPECTED MW: 27kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 96
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM TTSDLPAFWT VIPAAGVGSR MRADRPKQYL DLAGRTVIER TLDCFLEHPM LRGLVVCLAE DDPYWPGLDC AASRHVQRAA GGAERAGSVL NGLLRLLELG AQADDWVLVH DAARPNLTRG DLDRLLEELA EDPVGGLLAV PARDTLKRSD RDGRVSETID RSVVWLAYTP QMFRLGALHR ALADALVAGV AITDEASAHS RPTPSPKLVE GRADNLKITT PEDLLRLQRS FPH
Validated NT Sequence
cnnnnnannn ncnctanann attttgttta ctttaagaag gagatatacc atggctcacc accaccacca ccaccatatg acgacttccg accttcccgc cttctggacg gtgatccccg ccgccggcgt gggttcgcgc atgcgcgccg accgcccgaa gcaatatctc gatcttgccg gtcgcaccgt gatcgagcgt accctcgatt gtttcctcga acacccgatg ctcaggggcc tggtggtctg cctggccgag gacgatccct actggcccgg gctggactgc gccgccagcc ggcatgtgca gcgcgccgcc gggggcgccg agcgcgccgg ctcggtgttg aacggcttgc tgcgcctgct ggagttgggg gcacaggccg acgactgggt gctggtgcat gacgccgcgc ggccgaacct gacccgcggc gacctcgacc gcctgctcga agaactcgcc gaagatccgg tcggcgggct gctggcggtg ccggcgaggg acactctcaa gcgctccgac cgcgacggcc gggtcagcga aaccatcgac cgctcggtgg tctggctggc ctatacgccg cagatgttcc gcctcggcgc cctgcaccgg gcgctggccg atgcgttggt ggccggcgtg gcgatcaccg acgaggcttc ggcgcatagc cggcccactc catcgccgaa gctggtggaa gggcgcgccg acaacctgaa gatcaccacc ccggaagacc tgctgcgcct gcagcgcagt tttccgcact aagtgagtaa gataggatcc ggctg
Expected Protein Sequence
MAHHHHHHMT TSDLPAFWTV IPAAGVGSRM RADRPKQYLD LAGRTVIERT LDCFLEHPML RGLVVCLAED DPYWPGLDCA ASRHVQRAAG GAERAGSVLN GLLRLLELGA QADDWVLVHD AARPNLTRGD LDRLLEELAE DPVGGLLAVP ARDTLKRSDR DGRVSETIDR SVVWLAYTPQ MFRLGALHRA LADALVAGVA ITDEASAMEW AGYAPKLVEG RADNLKITTP EDLLRLQRSF PH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga cttccgacct tcccgccttc tggacggtga tccccgccgc cggcgtgggt tcgcgcatgc gcgccgaccg cccgaagcaa tatctcgatc ttgccggtcg caccgtgatc gagcgtaccc tcgattgttt cctcgaacac ccgatgctca ggggcctggt ggtctgcctg gccgaggacg atccctactg gcccgggctg gactgcgccg ccagccggca tgtgcagcgc gccgccgggg gcgccgagcg cgccggctcg gtgttgaacg gcttgctgcg cctgctggag ttgggggcac aggccgacga ctgggtgctg gtgcatgacg ccgcgcggcc gaacctgacc cgcggcgacc tcgaccgcct gctcgaagaa ctcgccgaag atccggtcgg cgggctgctg gcggtgccgg cgagggacac tctcaagcgc tccgaccgcg acggccgggt cagcgaaacc atcgaccgct cggtggtctg gctggcctat acgccgcaga tgttccgcct cggcgccctg caccgggcgc tggccgatgc gttggtggcc ggcgtggcga tcaccgacga ggcttcggcg atggagtggg ccggctatgc gccgaagctg gtggaagggc gcgccgacaa cctgaagatc accaccccgg aagacctgct gcgcctgcag cgcagttttc cgcactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat