PsaeA.00166.a
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (LpxC)
| CENTER ID: | PsaeA.00166.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00166.a.DG15.GE00589 | full length | 1 | 304 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00166.a.DG15.PD00471 | full length | 1 | 304 |
Structures
5UPG
DEPOSITED: 2/2/2017
DETERMINATION: XRay
CLONE: PsaeA.00166.a.DG15.GE00589
PROTEIN: PsaeA.00166.a.DG15.PD00471
5VWM
DEPOSITED: 5/22/2017
DETERMINATION: XRay
CLONE: PsaeA.00166.a.DG15.GE00589
PROTEIN: PsaeA.00166.a.DG15.PD00471
6CAX
DEPOSITED: 2/1/2018
DETERMINATION: XRay
CLONE: PsaeA.00166.a.DG15.GE00589
PROTEIN: PsaeA.00166.a.DG15.PD00442
6C9C
DEPOSITED: 1/26/2018
DETERMINATION: XRay
CLONE: PsaeA.00166.a.DG15.GE00589
PROTEIN: PsaeA.00166.a.DG15.PD00471
6E54
DEPOSITED: 7/19/2018
DETERMINATION: XRay
CLONE: PsaeA.00166.a.DG15.GE00589
PROTEIN: PsaeA.00166.a.DG15.PD00471
6DUI
DEPOSITED: 6/20/2018
DETERMINATION: XRay
CLONE: PsaeA.00166.a.DG15.GE00589
PROTEIN: PsaeA.00166.a.DG15.PD00471
Publications by SSGCID
Structure-kinetic relationship studies for the development of long residence time LpxC inhibitors
Edwards TE, Phan JN, Li Y, Basak S, Tao S, Daryaee F, Merino J, Gu C, Delker S, Walker S, Tonge P
Med Chem - 2023
volume 65, issue 17, pages 11854-11875
PMID: 36037447; PMCID: PMC10182817
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.4615 |
| RefSeq: | WP_003094111.1 |
| UniProt: | P47205 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIKQRTLKNI IRATGVGLHS GEKVYLTLKP APVDTGIVFC RTDLDPVVEI PARAENVGET TMSTTLVKGD VKVDTVEHLL SAMAGLGIDN AYVELSASEV PIMDGSAGPF VFLIQSAGLQ EQEAAKKFIR IKREVSVEEG DKRAVFVPFD GFKVSFEIDF DHPVFRGRTQ QASVDFSSTS FVKEVSRART FGFMRDIEYL RSQNLALGGS VENAIVVDEN RVLNEDGLRY EDEFVKHKIL DAIGDLYLLG NSLIGEFRGF KSGHALNNQL LRTLIADKDA WEVVTFEDAR TAPISYMRPA AAV
NT Sequence
atgatcaaac aacgcacctt gaagaacatc atccgggcta ctggcgtcgg tctgcactcg ggggaaaagg tttacctgac cctgaaaccg gcgccggtgg acaccggtat cgtgttctgc cgcaccgacc tggatccggt cgtggaaatc ccggcccggg ccgagaacgt cggcgaaacc accatgtcga ccaccctggt caagggtgac gtcaaagtgg atacggtgga gcacctgctc tcggccatgg ccggcctggg tatcgacaac gcctacgtcg agctgtccgc ttcggaagtg ccgatcatgg atggcagcgc tggtccgttc gtattcctga tccagtccgc cggattgcaa gagcaggaag ctgccaagaa gttcatccgc atcaagcgcg aagtcagcgt ggaagagggc gacaagcgcg ccgtcttcgt tccgttcgac ggcttcaagg tcagcttcga gatcgatttc gatcatccgg tcttccgtgg tcgcacccag caggcctcgg tcgatttctc cagtacttcc ttcgtcaagg aggtcagccg cgcccgtacc ttcgggttca tgcgcgacat cgagtacctg cgttcgcaga acctggcact cggcggtagc gtggagaacg cgatcgtggt cgacgagaac cgcgtgctca acgaagacgg cctgcgttac gaggacgagt tcgtcaagca caagatcctg gatgccatcg gcgacctgta tctgctcggc aacagtctta tcggcgagtt ccgtggcttc aagtccggcc atgccctgaa caaccaactg ctgcgtacgt tgatcgcaga caaggatgct tgggaagtgg tgaccttcga agacgcgcgt accgcgccta tttcctatat gcgcccggcg gcggcagtgt ag
Details for PsaeA.00166.a.DG15.GE00589
| HARVESTED ON: | 3/11/2016 |
| SEQUENCED ON: | 10/25/2017 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMIK QRTLKNIIRA TGVGLHSGEK VYLTLKPAPV DTGIVFSRTD LDPVVEIPAR AENVGETTMS TTLVKGDVKV DTVEHLLSAM AGLGIDNAYV ELSASEVPIM DGSAGPFVFL IQSAGLQEQE AAKKFIRIKR EVSVEEGDKR AVFVPFDGFK VSFEIDFDHP VFRGRTQQAS VDFSSTSFVK EVSRARTFGF MRDIEYLRSQ NLALGGSVEN AIVVDENRVL NEDGLRYEDE FVKHKILDAI GDLYLLGNSL IGEFRGFKSG HALNNQLLRT LIADKDAWEV VTFEDARTA
Validated NT Sequence
gtgcggtgcg ggcgtcttca aaagtcacga cttcccaggc gtctttatcg gcgatgagag tacgcagcag ttggttgttc agagcgtgac cagatttaaa accgcggaat tcaccgatca gagagttgcc taagaggtac agatcgccga tagcgtcgag aattttgtgt ttgacgaatt cgtcttcgta acgcagaccg tcctcgttga gaacacggtt ttcgtcgaca actatagcat tttcaacgct gccacccaga gccaggttct gagaacgcag gtactcgatg tcacgcataa aaccgaaggt acgagcgcgg gacacctcct ttacgaagct ggtggagctg aagtcaacgg aggcctgttg ggtacggcca cggaacactg ggtggtcgaa atcgatttca aaggacacct taaaaccgtc gaatggtacg aacacggcac gtttatcgcc ttcttcgaca gaaacttcgc gcttgatacg gatgaacttc ttagcggctt cttgctcctg gagaccggcg ctctggatca ggaacacaaa cgggccagcg gaaccgtcca tgatcggaac ttcagaagcg gagagttcta catatgcgtt gtcgatgccc agaccagcca tggcggacag caggtgttca acggtatcga ctttcacgtc accctttacc agagtggtgg acatagtagt ttcgccaacg ttttcagcac gggccgggat ctcaacaacc ggatctaaat cggtgcgact gaacacgatg ccagtgtcta ccggggcagg tttcagagtc aggtaaacct tttcaccaga gtgcagacca acgccggtag cgcgaataat gttcttcagg gtgcgctgtt tgatcatgga tccaccgatt tgttcacggt gtgcttcgat aatgtcgtta tcttccatgt ccagatcttc cggagtctgg tctgcctgaa tacggatacc gtcgtacaga aagcgcaggg aatccatttc cttgccctgg cgtttagcga atgcttccat caggcgacgc agcggggtgg tcttcttaat cttgaagaag atttcgctgc taccgtcgct aaccttcagg ttgatgtggg tctccggctt cacttccggt tttacttcgc cgctatggtg gtggtggtgg tgggacat
Expected Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMIK QRTLKNIIRA TGVGLHSGEK VYLTLKPAPV DTGIVFCRTD LDPVVEIPAR AENVGETTMS TTLVKGDVKV DTVEHLLSAM AGLGIDNAYV ELSASEVPIM DGSAGPFVFL IQSAGLQEQE AAKKFIRIKR EVSVEEGDKR AVFVPFDGFK VSFEIDFDHP VFRGRTQQAS VDFSSTSFVK EVSRARTFGF MRDIEYLRSQ NLALGGSVEN AIVVDENRVL NEDGLRYEDE FVKHKILDAI GDLYLLGNSL IGEFRGFKSG HALNNQLLRT LIADKDAWEV VTFEDARTAP ISYMRPAAAV
Full NT Sequence (Expression Vector + Insert)
atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg ctcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca tgtcccacca ccaccaccac catagcggcg aagtaaaacc ggaagtgaag ccggagaccc acatcaacct gaaggttagc gacggtagca gcgaaatctt cttcaagatt aagaagacca ccccgctgcg tcgcctgatg gaagcattcg ctaaacgcca gggcaaggaa atggattccc tgcgctttct gtacgacggt atccgtattc aggcagacca gactccggaa gatctggaca tggaagataa cgacattatc gaagcacacc gtgaacaaat cggtggatcc atgatcaaac agcgcaccct gaagaacatt attcgcgcta ccggcgttgg tctgcactct ggtgaaaagg tttacctgac tctgaaacct gccccggtag acactggcat cgtgttctgt cgcaccgatt tagatccggt tgttgagatc ccggcccgtg ctgaaaacgt tggcgaaact actatgtcca ccactctggt aaagggtgac gtgaaagtcg ataccgttga acacctgctg tccgccatgg ctggtctggg catcgacaac gcatatgtag aactctccgc ttctgaagtt ccgatcatgg acggttccgc tggcccgttt gtgttcctga tccagagcgc cggtctccag gagcaagaag ccgctaagaa gttcatccgt atcaagcgcg aagtttctgt cgaagaaggc gataaacgtg ccgtgttcgt accattcgac ggttttaagg tgtcctttga aatcgatttc gaccacccag tgttccgtgg ccgtacccaa caggcctccg ttgacttcag ctccaccagc ttcgtaaagg aggtgtcccg cgctcgtacc ttcggtttta tgcgtgacat cgagtacctg cgttctcaga acctggctct gggtggcagc gttgaaaatg ctatagttgt cgacgaaaac cgtgttctca acgaggacgg tctgcgttac gaagacgaat tcgtcaaaca caaaattctc gacgctatcg gcgatctgta cctcttaggc aactctctga tcggtgaatt ccgcggtttt aaatctggtc acgctctgaa caaccaactg ctgcgtactc tcatcgccga taaagacgcc tgggaagtcg tgacttttga agacgcccgc accgcaccaa tctcctacat gcgtccggct gccgctgtgt gataagcttg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaacttgaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat agggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagcaag gagatggcgc gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta tc
Details for PsaeA.00166.a.DG15.PD00471
| PURIFICATION DATe: | 10/26/2016 |
| CONCENTRATION: | 5mg/ml |
| OBSERVED MW: | 44kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25mM HEPES pH 8.0, 150mM NaCl, 2mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMIK QRTLKNIIRA TGVGLHSGEK VYLTLKPAPV DTGIVFSRTD LDPVVEIPAR AENVGETTMS TTLVKGDVKV DTVEHLLSAM AGLGIDNAYV ELSASEVPIM DGSAGPFVFL IQSAGLQEQE AAKKFIRIKR EVSVEEGDKR AVFVPFDGFK VSFEIDFDHP VFRGRTQQAS VDFSSTSFVK EVSRARTFGF MRDIEYLRSQ NLALGGSVEN AIVVDENRVL NEDGLRYEDE FVKHKILDAI GDLYLLGNSL IGEFRGFKSG HALNNQLLRT LIADKDAWEV VTFEDARTA
Validated NT Sequence
gtgcggtgcg ggcgtcttca aaagtcacga cttcccaggc gtctttatcg gcgatgagag tacgcagcag ttggttgttc agagcgtgac cagatttaaa accgcggaat tcaccgatca gagagttgcc taagaggtac agatcgccga tagcgtcgag aattttgtgt ttgacgaatt cgtcttcgta acgcagaccg tcctcgttga gaacacggtt ttcgtcgaca actatagcat tttcaacgct gccacccaga gccaggttct gagaacgcag gtactcgatg tcacgcataa aaccgaaggt acgagcgcgg gacacctcct ttacgaagct ggtggagctg aagtcaacgg aggcctgttg ggtacggcca cggaacactg ggtggtcgaa atcgatttca aaggacacct taaaaccgtc gaatggtacg aacacggcac gtttatcgcc ttcttcgaca gaaacttcgc gcttgatacg gatgaacttc ttagcggctt cttgctcctg gagaccggcg ctctggatca ggaacacaaa cgggccagcg gaaccgtcca tgatcggaac ttcagaagcg gagagttcta catatgcgtt gtcgatgccc agaccagcca tggcggacag caggtgttca acggtatcga ctttcacgtc accctttacc agagtggtgg acatagtagt ttcgccaacg ttttcagcac gggccgggat ctcaacaacc ggatctaaat cggtgcgact gaacacgatg ccagtgtcta ccggggcagg tttcagagtc aggtaaacct tttcaccaga gtgcagacca acgccggtag cgcgaataat gttcttcagg gtgcgctgtt tgatcatgga tccaccgatt tgttcacggt gtgcttcgat aatgtcgtta tcttccatgt ccagatcttc cggagtctgg tctgcctgaa tacggatacc gtcgtacaga aagcgcaggg aatccatttc cttgccctgg cgtttagcga atgcttccat caggcgacgc agcggggtgg tcttcttaat cttgaagaag atttcgctgc taccgtcgct aaccttcagg ttgatgtggg tctccggctt cacttccggt tttacttcgc cgctatggtg gtggtggtgg tgggacat
Expressed Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMIK QRTLKNIIRA TGVGLHSGEK VYLTLKPAPV DTGIVFCRTD LDPVVEIPAR AENVGETTMS TTLVKGDVKV DTVEHLLSAM AGLGIDNAYV ELSASEVPIM DGSAGPFVFL IQSAGLQEQE AAKKFIRIKR EVSVEEGDKR AVFVPFDGFK VSFEIDFDHP VFRGRTQQAS VDFSSTSFVK EVSRARTFGF MRDIEYLRSQ NLALGGSVEN AIVVDENRVL NEDGLRYEDE FVKHKILDAI GDLYLLGNSL IGEFRGFKSG HALNNQLLRT LIADKDAWEV VTFEDARTAP ISYMRPAAAV
Full NT Sequence (Expression Vector + Insert)
ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG CTCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCAGGAG GAATTCACCA TGTCCCACCA CCACCACCAC CATAGCGGCG AAGTAAAACC GGAAGTGAAG CCGGAGACCC ACATCAACCT GAAGGTTAGC GACGGTAGCA GCGAAATCTT CTTCAAGATT AAGAAGACCA CCCCGCTGCG TCGCCTGATG GAAGCATTCG CTAAACGCCA GGGCAAGGAA ATGGATTCCC TGCGCTTTCT GTACGACGGT ATCCGTATTC AGGCAGACCA GACTCCGGAA GATCTGGACA TGGAAGATAA CGACATTATC GAAGCACACC GTGAACAAAT CGGTGGATCC ATGATCAAAC AGCGCACCCT GAAGAACATT ATTCGCGCTA CCGGCGTTGG TCTGCACTCT GGTGAAAAGG TTTACCTGAC TCTGAAACCT GCCCCGGTAG ACACTGGCAT CGTGTTCTGT CGCACCGATT TAGATCCGGT TGTTGAGATC CCGGCCCGTG CTGAAAACGT TGGCGAAACT ACTATGTCCA CCACTCTGGT AAAGGGTGAC GTGAAAGTCG ATACCGTTGA ACACCTGCTG TCCGCCATGG CTGGTCTGGG CATCGACAAC GCATATGTAG AACTCTCCGC TTCTGAAGTT CCGATCATGG ACGGTTCCGC TGGCCCGTTT GTGTTCCTGA TCCAGAGCGC CGGTCTCCAG GAGCAAGAAG CCGCTAAGAA GTTCATCCGT ATCAAGCGCG AAGTTTCTGT CGAAGAAGGC GATAAACGTG CCGTGTTCGT ACCATTCGAC GGTTTTAAGG TGTCCTTTGA AATCGATTTC GACCACCCAG TGTTCCGTGG CCGTACCCAA CAGGCCTCCG TTGACTTCAG CTCCACCAGC TTCGTAAAGG AGGTGTCCCG CGCTCGTACC TTCGGTTTTA TGCGTGACAT CGAGTACCTG CGTTCTCAGA ACCTGGCTCT GGGTGGCAGC GTTGAAAATG CTATAGTTGT CGACGAAAAC CGTGTTCTCA ACGAGGACGG TCTGCGTTAC GAAGACGAAT TCGTCAAACA CAAAATTCTC GACGCTATCG GCGATCTGTA CCTCTTAGGC AACTCTCTGA TCGGTGAATT CCGCGGTTTT AAATCTGGTC ACGCTCTGAA CAACCAACTG CTGCGTACTC TCATCGCCGA TAAAGACGCC TGGGAAGTCG TGACTTTTGA AGACGCCCGC ACCGCACCAA TCTCCTACAT GCGTCCGGCT GCCGCTGTGT GATAAGCTTG GCTGTTTTGG CGGATGAGAG AAGATTTTCA GCCTGATACA GATTAAATCA GAACGCAGAA GCGGTCTGAT AAAACAGAAT TTGCCTGGCG GCAGTAGCGC GGTGGTCCCA CCTGACCCCA TGCCGAACTC AGAAGTGAAA CGCCGTAGCG CCGATGGTAG TGTGGGGTCT CCCCATGCGA GAGTAGGGAA CTGCCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGGGAGCG GATTTGAACG TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG CAGGACGCCC GCCATAAACT GCCAGGCATC AAATTAAGCA GAAGGCCATC CTGACGGATG GCCTTTTTGC GTTTCTACAA ACTCTTTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTTGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTTGAA CAACACTCAA CCCTATCTCG GGCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGTTTAC AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT AGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCAAG GAGATGGCGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC TGCTCATGTT TGACAGCTTA TC