PsaeA.00164.a

Arginine--tRNA ligase (EC 6.1.1.19) (Arginyl-tRNA synthetase) (ArgRS)

CENTER ID: PsaeA.00164.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00164.a.B1.GE38226 full length 1 587
PsaeA.00164.a.B2.GE40431 truncation 1 1 380
PsaeA.00164.a.B4.GE40433 truncation 3 27 587
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00164.a.B2.PW37947 truncation 1 1 380
PsaeA.00164.a.B1.PW38765 full length 1 587
PsaeA.00164.a.B1.PW38766 full length 1 587

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.5295
PGAT: pseudomonas:PA5051
RefSeq: NP_253738.1
UniProt: Q9HUC8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKDTIRQLIQ QALDQLTADG TLPAGLTPDI QVENTKDRSH GDFASNIAMM LAKPAGMKPR DLAARLVEAI PAHEQLAKVE IAGPGFLNFF QDHVWLAASL DRALADERLG VRKAGPAQRV VIDLSSPNLA KEMHVGHLRS TIIGDAVARV LEFLGDTVIR QNHVGDWGTQ FGMLLAYLEE QPVDAEAELH DLEVFYRAAK KRFDESPEFA DRARELVVKL QAGDPDCLRL WTRFNEISLS HCQKVYDRLG VKLSMADVMG ESAYNDDLAQ VVADLTAKGL LTEDNGALCV FLEEFKNAEG NPLPVIVQKA GGGYLYATTD LAAMRYRHNV LHADRVLYFV DQRQALHFQQ VFEVARRAGF VPAGMELEHM GFGTMNGADG RPFKTRDGGT VKLIDLLEEA ESRAYALVKE RNEQRAERGE EPFDEVQLRE IGRVVGIDSV KYADLSKHRT SDYSFNFELM LSFEGNTAPY LLYACTRVAS VFRKLGQGRE QLGGKIVLEQ PQELALAAQL AQFGDLINNV ALKGVPHLLC AYLYELAGLF SSFYEHCPIL TAEDPAQKDS RLRLAALTGR TLEQGLELLG LKTLERM
NT Sequence
atgaaagaca cgattcgcca gctgatccag caagccctcg accaactgac cgcggacggc accctgcccg ccggcctgac cccggacatc caggtggaga acaccaagga ccgtagccac ggcgatttcg ccagcaacat cgccatgatg ctggccaagc cggccgggat gaagccgcgc gacctggccg ccaggctggt cgaggcaatc cccgcccacg agcaactggc caaggtggag atcgccggtc ccggcttcct caacttcttc caggatcacg tctggctcgc cgccagcctc gaccgcgccc tcgccgacga acgtctcggc gtgcgcaagg caggcccggc gcagcgcgtg gtgatcgacc tgtcctcgcc gaacctggcc aaggaaatgc acgtcggcca cctgcgctcg accatcatcg gcgacgccgt cgcgcgcgtc ctcgagttcc tcggcgacac ggtgatccgg cagaaccatg tcggcgactg gggcacccag ttcggcatgc tgctcgccta cctggaagag caaccggtcg acgccgaggc cgagctgcac gacctggagg tcttctaccg cgcggcgaag aaacgcttcg acgagtctcc cgaattcgcc gaccgcgccc gcgaactggt agtcaagctg caggccggcg accccgactg cctgcgcctg tggacccgct tcaacgagat ttccctgtcg cactgccaga aggtctacga ccgtctcggc gtgaagctga gcatggccga cgtcatgggc gagagcgcct acaacgacga cctcgcccag gtggtcgccg acctgaccgc caagggcctg ctcaccgaag acaacggcgc gttgtgcgtc ttcctcgaag agttcaagaa cgccgagggc aatccgctcc cggtgatcgt gcagaaggcc ggcggcggct acctctacgc caccaccgac ctcgccgcca tgcgttatcg gcacaacgtg ctgcacgccg accgcgtgct gtacttcgtc gaccagcgcc aggccctgca cttccagcag gtcttcgaag tggcgcggcg cgccggtttc gttccggccg gcatggagct ggagcacatg ggcttcggca ccatgaacgg cgccgacggc cgtccgttca agacccgcga cggcggtacg gtgaagctga tcgacctgct ggaagaggcc gagagccgcg cctatgcgct ggtcaaggaa cgcaacgagc agcgcgccga gcgtggcgag gagccgttcg acgaggtcca gctgcgcgag atcggccggg tggtggggat cgactcggtg aagtacgccg acctgtccaa gcaccgcacc agcgactaca gcttcaactt cgaactgatg ctgagcttcg aaggcaacac cgcaccctac ctgctctacg cctgcacccg cgtggccagc gtcttccgca agctcggcca gggccgcgag caactgggcg ggaagatcgt cctcgagcag ccccaggagc tggccctggc cgcacagctg gcgcagttcg gcgacctgat caacaacgtc gcgctcaagg gcgtgccgca cctgctctgc gcctacctgt acgagttggc cgggctgttc tccagcttct acgagcattg cccgatcctc accgccgagg acccggcgca gaaggacagc cgcctgcgtc tcgccgcgct gaccggccgt accctggaac agggcctgga gctgctcggt ctgaagactc tggaacgcat gtaa
Details for PsaeA.00164.a.B1.GE38226
HARVESTED ON: 7/1/2014
SEQUENCED ON: 7/8/2014
EXPECTED MW: 66kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 58
PERCENT COVERAGE: 89
Validated AA Sequence
MMLAKPAGMK PRDLAARLVE AIPAHEQLAK VEIAGPGFLN FFQDHVWLAA SLDRALADER LGVRKAGPAQ RVVIDLSSPN LAKEMHVGHL RSTIIGDAVA RVLEFLGDTV IRQNHVGDWG TQFGMLLAYL EEQPVDAEAE LHDLEVFYRA AKKRFDESPE FADRARELVV KLQAGDPDCL RLWTRFNEIS LSHCQXVYDR LGVKLXMADV MGESAYNDDL AQVVADLTAK GLLTEDNGAL CVFLEEFKNA EGNPLPGDRA EGRRRLPLRH HRPRRHALSA QRAXXXPRAV LRRPAPGPAX PAGLXSGAAR RFRSGRHGAG AHGLRHHERR RRPSVQDPRR RYGEADRPAG RGREPRLCAG QGTQRAARRA WRGAVRRGPA ARDRPGGGDR LGEVRRPVQA PHQRLQLQLR TDAELRRQHR TLPALRLHPR GQRLPQARPG PRATGREDRP RAAPGAGPGR TAGAVRRPDQ QRRAQGRAAP ALRLPVRVGR AVLQLLRALP DPHRRGPGAE GQPPASRRAD RPYPGTGPGA A
Validated NT Sequence
gagcagctcc aggccctgtt ccagggtacg gccggtcagc gcggcgagac gcaggcggct gtccttctgc gccgggtcct cggcggtgag gatcgggcaa tgctcgtaga agctggagaa cagcccggcc aactcgtaca ggtaggcgca gagcaggtgc ggcacgccct tgagcgcgac gttgttgatc aggtcgccga actgcgccag ctgtgcggcc agggccagct cctggggctg ctcgaggacg atcttcccgc ccagttgctc gcggccctgg ccgagcttgc ggaagacgct ggccacgcgg gtgcaggcgt agagcaggta gggtgcggtg ttgccttcga agctcagcat cagttcgaag ttgaagctgt agtcgctggt gcggtgcttg gacaggtcgg cgtacttcac cgagtcgatc cccaccaccc ggccgatctc gcgcagctgg acctcgtcga acggctcctc gccacgctcg gcgcgctgct cgttgcgttc cttgaccagc gcataggcgc ggctctcggc ctcttccagc aggtcgatca gcttcaccgt accgccgtcg cgggtcttga acggacggcc gtcggcgccg ttcatggtgc cgaagcccat gtgctccagc tccatgccgg ccggaacgaa accggcgcgc cgcgccactt cnaagacctg ctggaantgc agggcctggc gctggtcgac gaagtacagc acgcggtcng ngtgnagcac gttgtgccga taacgcatgg cggcgaggtc ggtggtggcg tagaggtagc cgccgccggc cttctgcacg atcaccgggn agcggattgc cctcggcgtt cttgaactct tcgaggaaga cgcacaacgc gccgttgtct tcggtgagca ggcccttggc ggtcaggtcg gcgaccacct gggcgaggtc gtcgttgtag gcgctctcgc ccatgacgtc ggccatgntc agcttcacgc cgagacggtc gtagacnttc tggcagtgcg acagggaaat ctcgttgaag cgggtccaca ggcgcaggca gtcggggtcg ccggcctgca gcttgactac cagttcgcgg gcgcggtcgg cgaattcggg agactcgtcg aagcgtttct tcgccgcgcg gtagaagacc tccaggtcgt gcagctcggc ctcggcgtcg accggttgct cttccaggta ggcgagcagc atgccgaact gggtgcccca gtcgccgaca tggttctgcc ggatcaccgt gtcgccgagg aactcgagga cgcgcgcgac ggcgtcgccg atgatggtcg agcgcaggtg gccgacgtgc atttccttgg ccaggttcgg cgaggacagg tcgatcacca cgcgctgcgc cgggcctgcc ttgcgcacgc cgagacgttc gtcggcgagg gcgcggtcga ggctggcggc gagccagacg tgatcctgga agaagttgag gaagccggga ccggcgatct ccaccttggc cagttgctcg tgggcgggga ttgcctcgac cagcctggcg gccaggtcgc gcggcttcat cccggccggc ttggccagca tcatggcgat gttgctggcg aaatcgccgt ggctacggtc cttggtgttc tccacctgga tgtccggggt caggccggcg ggcagggtgc cgtccgcggt cagttggtcg agggcttgct ggatcagctg gcgaatcgtg tcttt
Expected Protein Sequence
MAHHHHHHMK DTIRQLIQQA LDQLTADGTL PAGLTPDIQV ENTKDRSHGD FASNIAMMLA KPAGMKPRDL AARLVEAIPA HEQLAKVEIA GPGFLNFFQD HVWLAASLDR ALADERLGVR KAGPAQRVVI DLSSPNLAKE MHVGHLRSTI IGDAVARVLE FLGDTVIRQN HVGDWGTQFG MLLAYLEEQP VDAEAELHDL EVFYRAAKKR FDESPEFADR ARELVVKLQA GDPDCLRLWT RFNEISLSHC QKVYDRLGVK LSMADVMGES AYNDDLAQVV ADLTAKGLLT EDNGALCVFL EEFKNAEGNP LPVIVQKAGG GYLYATTDLA AMRYRHNVLH ADRVLYFVDQ RQALHFQQVF EVARRAGFVP AGMELEHMGF GTMNGADGRP FKTRDGGTVK LIDLLEEAES RAYALVKERN EQRAERGEEP FDEVQLREIG RVVGIDSVKY ADLSKHRTSD YSFNFELMLS FEGNTAPYLL YACTRVASVF RKLGQGREQL GGKIVLEQPQ ELALAAQLAQ FGDLINNVAL KGVPHLLCAY LYELAGLFSS FYEHCPILTA EDPAQKDSRL RLAALTGRTL EQGLELLGLK TLERM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag acacgattcg ccagctgatc cagcaagccc tcgaccaact gaccgcggac ggcaccctgc ccgccggcct gaccccggac atccaggtgg agaacaccaa ggaccgtagc cacggcgatt tcgccagcaa catcgccatg atgctggcca agccggccgg gatgaagccg cgcgacctgg ccgccaggct ggtcgaggca atccccgccc acgagcaact ggccaaggtg gagatcgccg gtcccggctt cctcaacttc ttccaggatc acgtctggct cgccgccagc ctcgaccgcg ccctcgccga cgaacgtctc ggcgtgcgca aggcaggccc ggcgcagcgc gtggtgatcg acctgtcctc gccgaacctg gccaaggaaa tgcacgtcgg ccacctgcgc tcgaccatca tcggcgacgc cgtcgcgcgc gtcctcgagt tcctcggcga cacggtgatc cggcagaacc atgtcggcga ctggggcacc cagttcggca tgctgctcgc ctacctggaa gagcaaccgg tcgacgccga ggccgagctg cacgacctgg aggtcttcta ccgcgcggcg aagaaacgct tcgacgagtc tcccgaattc gccgaccgcg cccgcgaact ggtagtcaag ctgcaggccg gcgaccccga ctgcctgcgc ctgtggaccc gcttcaacga gatttccctg tcgcactgcc agaaggtcta cgaccgtctc ggcgtgaagc tgagcatggc cgacgtcatg ggcgagagcg cctacaacga cgacctcgcc caggtggtcg ccgacctgac cgccaagggc ctgctcaccg aagacaacgg cgcgttgtgc gtcttcctcg aagagttcaa gaacgccgag ggcaatccgc tcccggtgat cgtgcagaag gccggcggcg gctacctcta cgccaccacc gacctcgccg ccatgcgtta tcggcacaac gtgctgcacg ccgaccgcgt gctgtacttc gtcgaccagc gccaggccct gcacttccag caggtcttcg aagtggcgcg gcgcgccggt ttcgttccgg ccggcatgga gctggagcac atgggcttcg gcaccatgaa cggcgccgac ggccgtccgt tcaagacccg cgacggcggt acggtgaagc tgatcgacct gctggaagag gccgagagcc gcgcctatgc gctggtcaag gaacgcaacg agcagcgcgc cgagcgtggc gaggagccgt tcgacgaggt ccagctgcgc gagatcggcc gggtggtggg gatcgactcg gtgaagtacg ccgacctgtc caagcaccgc accagcgact acagcttcaa cttcgaactg atgctgagct tcgaaggcaa caccgcaccc tacctgctct acgcctgcac ccgcgtggcc agcgtcttcc gcaagctcgg ccagggccgc gagcaactgg gcgggaagat cgtcctcgag cagccccagg agctggccct ggccgcacag ctggcgcagt tcggcgacct gatcaacaac gtcgcgctca agggcgtgcc gcacctgctc tgcgcctacc tgtacgagtt ggccgggctg ttctccagct tctacgagca ttgcccgatc ctcaccgccg aggacccggc gcagaaggac agccgcctgc gtctcgccgc gctgaccggc cgtaccctgg aacagggcct ggagctgctc ggtctgaaga ctctggaacg catgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for PsaeA.00164.a.B2.GE40431
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK DTIRQLIQQA LDQLTADGTL PAGLTPDIQV ENTKDRSHGD FASNIAMMLA KPAGMKPRDL AARLVEAIPA HEQLAKVEIA GPGFLNFFQD HVWLAASLDR ALADERLGVR KAGPAQRVVI DLSSPNLAKE MHVGHLRSTI IGDAVARVLE FLGDTVIRQN HVGDWGTQFG MLLAYLEEQP VDAEAELHDL EVFYRAAKKR FDESPEFADR ARELVVKLQA GDPDCLRLWT RFNEISLSHC QKVYDRLGVK LSMADVMGES AYNDDLAQVV ADLTAKGLLT EDNGALCVFL EEFKNAEGNP LPVIVQKAGG GYLYATTDLA AMRYRHNVLH ADRVLYFVDQ RQALHFQQVF EVARRAGFVP AGMELEHMGF GTMNGADG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag acacgattcg ccagctgatc cagcaagccc tcgaccaact gaccgcggac ggcaccctgc ccgccggcct gaccccggac atccaggtgg agaacaccaa ggaccgtagc cacggcgatt tcgccagcaa catcgccatg atgctggcca agccggccgg gatgaagccg cgcgacctgg ccgccaggct ggtcgaggca atccccgccc acgagcaact ggccaaggtg gagatcgccg gtcccggctt cctcaacttc ttccaggatc acgtctggct cgccgccagc ctcgaccgcg ccctcgccga cgaacgtctc ggcgtgcgca aggcaggccc ggcgcagcgc gtggtgatcg acctgtcctc gccgaacctg gccaaggaaa tgcacgtcgg ccacctgcgc tcgaccatca tcggcgacgc cgtcgcgcgc gtcctcgagt tcctcggcga cacggtgatc cggcagaacc atgtcggcga ctggggcacc cagttcggca tgctgctcgc ctacctggaa gagcaaccgg tcgacgccga ggccgagctg cacgacctgg aggtcttcta ccgcgcggcg aagaaacgct tcgacgagtc tcccgaattc gccgaccgcg cccgcgaact ggtagtcaag ctgcaggccg gcgaccccga ctgcctgcgc ctgtggaccc gcttcaacga gatttccctg tcgcactgcc agaaggtcta cgaccgtctc ggcgtgaagc tgagcatggc cgacgtcatg ggcgagagcg cctacaacga cgacctcgcc caggtggtcg ccgacctgac cgccaagggc ctgctcaccg aagacaacgg cgcgttgtgc gtcttcctcg aagagttcaa gaacgccgag ggcaatccgc tcccggtgat cgtgcagaag gccggcggcg gctacctcta cgccaccacc gacctcgccg ccatgcgtta tcggcacaac gtgctgcacg ccgaccgcgt gctgtacttc gtcgaccagc gccaggccct gcacttccag caggtcttcg aagtggcgcg gcgcgccggt ttcgttccgg ccggcatgga gctggagcac atgggcttcg gcaccatgaa cggcgccgac ggctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PsaeA.00164.a.B4.GE40433
HARVESTED ON: data unavailable
SEQUENCED ON: 7/18/2016
EXPECTED MW: 63kDa
OBSERVED MW: 63kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHTP DIQVENTKDR SHGDFASNIA MMLAKPAGMK PRDLAARLVE AIPAHEQLAK VEIAGPGFLN FFQDHVWLAA SLDRALADER LGVRKAGPAQ RVVIDLSSPN LAKEMHVGHL RSTIIGDAVA RVLEFLGDTV IRQNHVGDWG TQFGMLLAYL EEQPVDAEAE LHDLEVFYRA AKKRFDESPE FADRARELVV KLQAGDPDCL RLWTRFNEIS LSHCQKVYDR LGVKLSMADV MGESAYNDDL AQVVADLTAK GLLTEDNGAL CVFLEEFKNA EGNPLPVIVQ KAGGGYLYAT TDLAAMRYRH NVLHADRVLY FVDQRQALHF QQVFEVARRA GFVPAGMELE HMGFGTMNGA DGRPFKTRDG GTVKLIDLLE EAESRAYALV KERNEQRAER GEEPFDEVQL REIGRVVGID SVKYADLSKH RTSDYSFNFE LMLSFEGNTA PYLLYACTRV ASVFRKLGQG REQLGGKIVL EQPQELALAA QLAQFGDLIN NVALKGVPHL LCAYLYELAG LFSSFYEHCP ILTAEDPAQK DSRLRLAALT GRTLEQGLEL LGLKTLERM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataccccgg acatccaggt ggagaacacc aaggaccgta gccacggcga tttcgccagc aacatcgcca tgatgctggc caagccggcc gggatgaagc cgcgcgacct ggccgccagg ctggtcgagg caatccccgc ccacgagcaa ctggccaagg tggagatcgc cggtcccggc ttcctcaact tcttccagga tcacgtctgg ctcgccgcca gcctcgaccg cgccctcgcc gacgaacgtc tcggcgtgcg caaggcaggc ccggcgcagc gcgtggtgat cgacctgtcc tcgccgaacc tggccaagga aatgcacgtc ggccacctgc gctcgaccat catcggcgac gccgtcgcgc gcgtcctcga gttcctcggc gacacggtga tccggcagaa ccatgtcggc gactggggca cccagttcgg catgctgctc gcctacctgg aagagcaacc ggtcgacgcc gaggccgagc tgcacgacct ggaggtcttc taccgcgcgg cgaagaaacg cttcgacgag tctcccgaat tcgccgaccg cgcccgcgaa ctggtagtca agctgcaggc cggcgacccc gactgcctgc gcctgtggac ccgcttcaac gagatttccc tgtcgcactg ccagaaggtc tacgaccgtc tcggcgtgaa gctgagcatg gccgacgtca tgggcgagag cgcctacaac gacgacctcg cccaggtggt cgccgacctg accgccaagg gcctgctcac cgaagacaac ggcgcgttgt gcgtcttcct cgaagagttc aagaacgccg agggcaatcc gctcccggtg atcgtgcaga aggccggcgg cggctacctc tacgccacca ccgacctcgc cgccatgcgt tatcggcaca acgtgctgca cgccgaccgc gtgctgtact tcgtcgacca gcgccaggcc ctgcacttcc agcaggtctt cgaagtggcg cggcgcgccg gtttcgttcc ggccggcatg gagctggagc acatgggctt cggcaccatg aacggcgccg acggccgtcc gttcaagacc cgcgacggcg gtacggtgaa gctgatcgac ctgctggaag aggccgagag ccgcgcctat gcgctggtca aggaacgcaa cgagcagcgc gccgagcgtg gcgaggagcc gttcgacgag gtccagctgc gcgagatcgg ccgggtggtg gggatcgact cggtgaagta cgccgacctg tccaagcacc gcaccagcga ctacagcttc aacttcgaac tgatgctgag cttcgaaggc aacaccgcac cctacctgct ctacgcctgc acccgcgtgg ccagcgtctt ccgcaagctc ggccagggcc gcgagcaact gggcgggaag atcgtcctcg agcagcccca ggagctggcc ctggccgcac agctggcgca gttcggcgac ctgatcaaca acgtcgcgct caagggcgtg ccgcacctgc tctgcgccta cctgtacgag ttggccgggc tgttctccag cttctacgag cattgcccga tcctcaccgc cgaggacccg gcgcagaagg acagccgcct gcgtctcgcc gcgctgaccg gccgtaccct ggaacagggc ctggagctgc tcggtctgaa gactctggaa cgcatgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for PsaeA.00164.a.B2.PW37947
PURIFICATION DATe: 10/17/2016
CONCENTRATION: 23.09mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK DTIRQLIQQA LDQLTADGTL PAGLTPDIQV ENTKDRSHGD FASNIAMMLA KPAGMKPRDL AARLVEAIPA HEQLAKVEIA GPGFLNFFQD HVWLAASLDR ALADERLGVR KAGPAQRVVI DLSSPNLAKE MHVGHLRSTI IGDAVARVLE FLGDTVIRQN HVGDWGTQFG MLLAYLEEQP VDAEAELHDL EVFYRAAKKR FDESPEFADR ARELVVKLQA GDPDCLRLWT RFNEISLSHC QKVYDRLGVK LSMADVMGES AYNDDLAQVV ADLTAKGLLT EDNGALCVFL EEFKNAEGNP LPVIVQKAGG GYLYATTDLA AMRYRHNVLH ADRVLYFVDQ RQALHFQQVF EVARRAGFVP AGMELEHMGF GTMNGADG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAG ACACGATTCG CCAGCTGATC CAGCAAGCCC TCGACCAACT GACCGCGGAC GGCACCCTGC CCGCCGGCCT GACCCCGGAC ATCCAGGTGG AGAACACCAA GGACCGTAGC CACGGCGATT TCGCCAGCAA CATCGCCATG ATGCTGGCCA AGCCGGCCGG GATGAAGCCG CGCGACCTGG CCGCCAGGCT GGTCGAGGCA ATCCCCGCCC ACGAGCAACT GGCCAAGGTG GAGATCGCCG GTCCCGGCTT CCTCAACTTC TTCCAGGATC ACGTCTGGCT CGCCGCCAGC CTCGACCGCG CCCTCGCCGA CGAACGTCTC GGCGTGCGCA AGGCAGGCCC GGCGCAGCGC GTGGTGATCG ACCTGTCCTC GCCGAACCTG GCCAAGGAAA TGCACGTCGG CCACCTGCGC TCGACCATCA TCGGCGACGC CGTCGCGCGC GTCCTCGAGT TCCTCGGCGA CACGGTGATC CGGCAGAACC ATGTCGGCGA CTGGGGCACC CAGTTCGGCA TGCTGCTCGC CTACCTGGAA GAGCAACCGG TCGACGCCGA GGCCGAGCTG CACGACCTGG AGGTCTTCTA CCGCGCGGCG AAGAAACGCT TCGACGAGTC TCCCGAATTC GCCGACCGCG CCCGCGAACT GGTAGTCAAG CTGCAGGCCG GCGACCCCGA CTGCCTGCGC CTGTGGACCC GCTTCAACGA GATTTCCCTG TCGCACTGCC AGAAGGTCTA CGACCGTCTC GGCGTGAAGC TGAGCATGGC CGACGTCATG GGCGAGAGCG CCTACAACGA CGACCTCGCC CAGGTGGTCG CCGACCTGAC CGCCAAGGGC CTGCTCACCG AAGACAACGG CGCGTTGTGC GTCTTCCTCG AAGAGTTCAA GAACGCCGAG GGCAATCCGC TCCCGGTGAT CGTGCAGAAG GCCGGCGGCG GCTACCTCTA CGCCACCACC GACCTCGCCG CCATGCGTTA TCGGCACAAC GTGCTGCACG CCGACCGCGT GCTGTACTTC GTCGACCAGC GCCAGGCCCT GCACTTCCAG CAGGTCTTCG AAGTGGCGCG GCGCGCCGGT TTCGTTCCGG CCGGCATGGA GCTGGAGCAC ATGGGCTTCG GCACCATGAA CGGCGCCGAC GGCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for PsaeA.00164.a.B1.PW38765
PURIFICATION DATe: 12/6/2019
CONCENTRATION: 9.22mg/ml
OBSERVED MW: 65kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 58
PERCENT COVERAGE: 89
Protocol Notes
notes unavailable
Validated AA Sequence
MMLAKPAGMK PRDLAARLVE AIPAHEQLAK VEIAGPGFLN FFQDHVWLAA SLDRALADER LGVRKAGPAQ RVVIDLSSPN LAKEMHVGHL RSTIIGDAVA RVLEFLGDTV IRQNHVGDWG TQFGMLLAYL EEQPVDAEAE LHDLEVFYRA AKKRFDESPE FADRARELVV KLQAGDPDCL RLWTRFNEIS LSHCQXVYDR LGVKLXMADV MGESAYNDDL AQVVADLTAK GLLTEDNGAL CVFLEEFKNA EGNPLPGDRA EGRRRLPLRH HRPRRHALSA QRAXXXPRAV LRRPAPGPAX PAGLXSGAAR RFRSGRHGAG AHGLRHHERR RRPSVQDPRR RYGEADRPAG RGREPRLCAG QGTQRAARRA WRGAVRRGPA ARDRPGGGDR LGEVRRPVQA PHQRLQLQLR TDAELRRQHR TLPALRLHPR GQRLPQARPG PRATGREDRP RAAPGAGPGR TAGAVRRPDQ QRRAQGRAAP ALRLPVRVGR AVLQLLRALP DPHRRGPGAE GQPPASRRAD RPYPGTGPGA A
Validated NT Sequence
gagcagctcc aggccctgtt ccagggtacg gccggtcagc gcggcgagac gcaggcggct gtccttctgc gccgggtcct cggcggtgag gatcgggcaa tgctcgtaga agctggagaa cagcccggcc aactcgtaca ggtaggcgca gagcaggtgc ggcacgccct tgagcgcgac gttgttgatc aggtcgccga actgcgccag ctgtgcggcc agggccagct cctggggctg ctcgaggacg atcttcccgc ccagttgctc gcggccctgg ccgagcttgc ggaagacgct ggccacgcgg gtgcaggcgt agagcaggta gggtgcggtg ttgccttcga agctcagcat cagttcgaag ttgaagctgt agtcgctggt gcggtgcttg gacaggtcgg cgtacttcac cgagtcgatc cccaccaccc ggccgatctc gcgcagctgg acctcgtcga acggctcctc gccacgctcg gcgcgctgct cgttgcgttc cttgaccagc gcataggcgc ggctctcggc ctcttccagc aggtcgatca gcttcaccgt accgccgtcg cgggtcttga acggacggcc gtcggcgccg ttcatggtgc cgaagcccat gtgctccagc tccatgccgg ccggaacgaa accggcgcgc cgcgccactt cnaagacctg ctggaantgc agggcctggc gctggtcgac gaagtacagc acgcggtcng ngtgnagcac gttgtgccga taacgcatgg cggcgaggtc ggtggtggcg tagaggtagc cgccgccggc cttctgcacg atcaccgggn agcggattgc cctcggcgtt cttgaactct tcgaggaaga cgcacaacgc gccgttgtct tcggtgagca ggcccttggc ggtcaggtcg gcgaccacct gggcgaggtc gtcgttgtag gcgctctcgc ccatgacgtc ggccatgntc agcttcacgc cgagacggtc gtagacnttc tggcagtgcg acagggaaat ctcgttgaag cgggtccaca ggcgcaggca gtcggggtcg ccggcctgca gcttgactac cagttcgcgg gcgcggtcgg cgaattcggg agactcgtcg aagcgtttct tcgccgcgcg gtagaagacc tccaggtcgt gcagctcggc ctcggcgtcg accggttgct cttccaggta ggcgagcagc atgccgaact gggtgcccca gtcgccgaca tggttctgcc ggatcaccgt gtcgccgagg aactcgagga cgcgcgcgac ggcgtcgccg atgatggtcg agcgcaggtg gccgacgtgc atttccttgg ccaggttcgg cgaggacagg tcgatcacca cgcgctgcgc cgggcctgcc ttgcgcacgc cgagacgttc gtcggcgagg gcgcggtcga ggctggcggc gagccagacg tgatcctgga agaagttgag gaagccggga ccggcgatct ccaccttggc cagttgctcg tgggcgggga ttgcctcgac cagcctggcg gccaggtcgc gcggcttcat cccggccggc ttggccagca tcatggcgat gttgctggcg aaatcgccgt ggctacggtc cttggtgttc tccacctgga tgtccggggt caggccggcg ggcagggtgc cgtccgcggt cagttggtcg agggcttgct ggatcagctg gcgaatcgtg tcttt
Expressed Protein Sequence
MAHHHHHHMK DTIRQLIQQA LDQLTADGTL PAGLTPDIQV ENTKDRSHGD FASNIAMMLA KPAGMKPRDL AARLVEAIPA HEQLAKVEIA GPGFLNFFQD HVWLAASLDR ALADERLGVR KAGPAQRVVI DLSSPNLAKE MHVGHLRSTI IGDAVARVLE FLGDTVIRQN HVGDWGTQFG MLLAYLEEQP VDAEAELHDL EVFYRAAKKR FDESPEFADR ARELVVKLQA GDPDCLRLWT RFNEISLSHC QKVYDRLGVK LSMADVMGES AYNDDLAQVV ADLTAKGLLT EDNGALCVFL EEFKNAEGNP LPVIVQKAGG GYLYATTDLA AMRYRHNVLH ADRVLYFVDQ RQALHFQQVF EVARRAGFVP AGMELEHMGF GTMNGADGRP FKTRDGGTVK LIDLLEEAES RAYALVKERN EQRAERGEEP FDEVQLREIG RVVGIDSVKY ADLSKHRTSD YSFNFELMLS FEGNTAPYLL YACTRVASVF RKLGQGREQL GGKIVLEQPQ ELALAAQLAQ FGDLINNVAL KGVPHLLCAY LYELAGLFSS FYEHCPILTA EDPAQKDSRL RLAALTGRTL EQGLELLGLK TLERM
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAG ACACGATTCG CCAGCTGATC CAGCAAGCCC TCGACCAACT GACCGCGGAC GGCACCCTGC CCGCCGGCCT GACCCCGGAC ATCCAGGTGG AGAACACCAA GGACCGTAGC CACGGCGATT TCGCCAGCAA CATCGCCATG ATGCTGGCCA AGCCGGCCGG GATGAAGCCG CGCGACCTGG CCGCCAGGCT GGTCGAGGCA ATCCCCGCCC ACGAGCAACT GGCCAAGGTG GAGATCGCCG GTCCCGGCTT CCTCAACTTC TTCCAGGATC ACGTCTGGCT CGCCGCCAGC CTCGACCGCG CCCTCGCCGA CGAACGTCTC GGCGTGCGCA AGGCAGGCCC GGCGCAGCGC GTGGTGATCG ACCTGTCCTC GCCGAACCTG GCCAAGGAAA TGCACGTCGG CCACCTGCGC TCGACCATCA TCGGCGACGC CGTCGCGCGC GTCCTCGAGT TCCTCGGCGA CACGGTGATC CGGCAGAACC ATGTCGGCGA CTGGGGCACC CAGTTCGGCA TGCTGCTCGC CTACCTGGAA GAGCAACCGG TCGACGCCGA GGCCGAGCTG CACGACCTGG AGGTCTTCTA CCGCGCGGCG AAGAAACGCT TCGACGAGTC TCCCGAATTC GCCGACCGCG CCCGCGAACT GGTAGTCAAG CTGCAGGCCG GCGACCCCGA CTGCCTGCGC CTGTGGACCC GCTTCAACGA GATTTCCCTG TCGCACTGCC AGAAGGTCTA CGACCGTCTC GGCGTGAAGC TGAGCATGGC CGACGTCATG GGCGAGAGCG CCTACAACGA CGACCTCGCC CAGGTGGTCG CCGACCTGAC CGCCAAGGGC CTGCTCACCG AAGACAACGG CGCGTTGTGC GTCTTCCTCG AAGAGTTCAA GAACGCCGAG GGCAATCCGC TCCCGGTGAT CGTGCAGAAG GCCGGCGGCG GCTACCTCTA CGCCACCACC GACCTCGCCG CCATGCGTTA TCGGCACAAC GTGCTGCACG CCGACCGCGT GCTGTACTTC GTCGACCAGC GCCAGGCCCT GCACTTCCAG CAGGTCTTCG AAGTGGCGCG GCGCGCCGGT TTCGTTCCGG CCGGCATGGA GCTGGAGCAC ATGGGCTTCG GCACCATGAA CGGCGCCGAC GGCCGTCCGT TCAAGACCCG CGACGGCGGT ACGGTGAAGC TGATCGACCT GCTGGAAGAG GCCGAGAGCC GCGCCTATGC GCTGGTCAAG GAACGCAACG AGCAGCGCGC CGAGCGTGGC GAGGAGCCGT TCGACGAGGT CCAGCTGCGC GAGATCGGCC GGGTGGTGGG GATCGACTCG GTGAAGTACG CCGACCTGTC CAAGCACCGC ACCAGCGACT ACAGCTTCAA CTTCGAACTG ATGCTGAGCT TCGAAGGCAA CACCGCACCC TACCTGCTCT ACGCCTGCAC CCGCGTGGCC AGCGTCTTCC GCAAGCTCGG CCAGGGCCGC GAGCAACTGG GCGGGAAGAT CGTCCTCGAG CAGCCCCAGG AGCTGGCCCT GGCCGCACAG CTGGCGCAGT TCGGCGACCT GATCAACAAC GTCGCGCTCA AGGGCGTGCC GCACCTGCTC TGCGCCTACC TGTACGAGTT GGCCGGGCTG TTCTCCAGCT TCTACGAGCA TTGCCCGATC CTCACCGCCG AGGACCCGGC GCAGAAGGAC AGCCGCCTGC GTCTCGCCGC GCTGACCGGC CGTACCCTGG AACAGGGCCT GGAGCTGCTC GGTCTGAAGA CTCTGGAACG CATGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT
Details for PsaeA.00164.a.B1.PW38766
PURIFICATION DATe: 12/6/2019
CONCENTRATION: 12mg/ml
OBSERVED MW: 65kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 58
PERCENT COVERAGE: 89
Protocol Notes
notes unavailable
Validated AA Sequence
MMLAKPAGMK PRDLAARLVE AIPAHEQLAK VEIAGPGFLN FFQDHVWLAA SLDRALADER LGVRKAGPAQ RVVIDLSSPN LAKEMHVGHL RSTIIGDAVA RVLEFLGDTV IRQNHVGDWG TQFGMLLAYL EEQPVDAEAE LHDLEVFYRA AKKRFDESPE FADRARELVV KLQAGDPDCL RLWTRFNEIS LSHCQXVYDR LGVKLXMADV MGESAYNDDL AQVVADLTAK GLLTEDNGAL CVFLEEFKNA EGNPLPGDRA EGRRRLPLRH HRPRRHALSA QRAXXXPRAV LRRPAPGPAX PAGLXSGAAR RFRSGRHGAG AHGLRHHERR RRPSVQDPRR RYGEADRPAG RGREPRLCAG QGTQRAARRA WRGAVRRGPA ARDRPGGGDR LGEVRRPVQA PHQRLQLQLR TDAELRRQHR TLPALRLHPR GQRLPQARPG PRATGREDRP RAAPGAGPGR TAGAVRRPDQ QRRAQGRAAP ALRLPVRVGR AVLQLLRALP DPHRRGPGAE GQPPASRRAD RPYPGTGPGA A
Validated NT Sequence
gagcagctcc aggccctgtt ccagggtacg gccggtcagc gcggcgagac gcaggcggct gtccttctgc gccgggtcct cggcggtgag gatcgggcaa tgctcgtaga agctggagaa cagcccggcc aactcgtaca ggtaggcgca gagcaggtgc ggcacgccct tgagcgcgac gttgttgatc aggtcgccga actgcgccag ctgtgcggcc agggccagct cctggggctg ctcgaggacg atcttcccgc ccagttgctc gcggccctgg ccgagcttgc ggaagacgct ggccacgcgg gtgcaggcgt agagcaggta gggtgcggtg ttgccttcga agctcagcat cagttcgaag ttgaagctgt agtcgctggt gcggtgcttg gacaggtcgg cgtacttcac cgagtcgatc cccaccaccc ggccgatctc gcgcagctgg acctcgtcga acggctcctc gccacgctcg gcgcgctgct cgttgcgttc cttgaccagc gcataggcgc ggctctcggc ctcttccagc aggtcgatca gcttcaccgt accgccgtcg cgggtcttga acggacggcc gtcggcgccg ttcatggtgc cgaagcccat gtgctccagc tccatgccgg ccggaacgaa accggcgcgc cgcgccactt cnaagacctg ctggaantgc agggcctggc gctggtcgac gaagtacagc acgcggtcng ngtgnagcac gttgtgccga taacgcatgg cggcgaggtc ggtggtggcg tagaggtagc cgccgccggc cttctgcacg atcaccgggn agcggattgc cctcggcgtt cttgaactct tcgaggaaga cgcacaacgc gccgttgtct tcggtgagca ggcccttggc ggtcaggtcg gcgaccacct gggcgaggtc gtcgttgtag gcgctctcgc ccatgacgtc ggccatgntc agcttcacgc cgagacggtc gtagacnttc tggcagtgcg acagggaaat ctcgttgaag cgggtccaca ggcgcaggca gtcggggtcg ccggcctgca gcttgactac cagttcgcgg gcgcggtcgg cgaattcggg agactcgtcg aagcgtttct tcgccgcgcg gtagaagacc tccaggtcgt gcagctcggc ctcggcgtcg accggttgct cttccaggta ggcgagcagc atgccgaact gggtgcccca gtcgccgaca tggttctgcc ggatcaccgt gtcgccgagg aactcgagga cgcgcgcgac ggcgtcgccg atgatggtcg agcgcaggtg gccgacgtgc atttccttgg ccaggttcgg cgaggacagg tcgatcacca cgcgctgcgc cgggcctgcc ttgcgcacgc cgagacgttc gtcggcgagg gcgcggtcga ggctggcggc gagccagacg tgatcctgga agaagttgag gaagccggga ccggcgatct ccaccttggc cagttgctcg tgggcgggga ttgcctcgac cagcctggcg gccaggtcgc gcggcttcat cccggccggc ttggccagca tcatggcgat gttgctggcg aaatcgccgt ggctacggtc cttggtgttc tccacctgga tgtccggggt caggccggcg ggcagggtgc cgtccgcggt cagttggtcg agggcttgct ggatcagctg gcgaatcgtg tcttt
Expressed Protein Sequence
MAHHHHHHMK DTIRQLIQQA LDQLTADGTL PAGLTPDIQV ENTKDRSHGD FASNIAMMLA KPAGMKPRDL AARLVEAIPA HEQLAKVEIA GPGFLNFFQD HVWLAASLDR ALADERLGVR KAGPAQRVVI DLSSPNLAKE MHVGHLRSTI IGDAVARVLE FLGDTVIRQN HVGDWGTQFG MLLAYLEEQP VDAEAELHDL EVFYRAAKKR FDESPEFADR ARELVVKLQA GDPDCLRLWT RFNEISLSHC QKVYDRLGVK LSMADVMGES AYNDDLAQVV ADLTAKGLLT EDNGALCVFL EEFKNAEGNP LPVIVQKAGG GYLYATTDLA AMRYRHNVLH ADRVLYFVDQ RQALHFQQVF EVARRAGFVP AGMELEHMGF GTMNGADGRP FKTRDGGTVK LIDLLEEAES RAYALVKERN EQRAERGEEP FDEVQLREIG RVVGIDSVKY ADLSKHRTSD YSFNFELMLS FEGNTAPYLL YACTRVASVF RKLGQGREQL GGKIVLEQPQ ELALAAQLAQ FGDLINNVAL KGVPHLLCAY LYELAGLFSS FYEHCPILTA EDPAQKDSRL RLAALTGRTL EQGLELLGLK TLERM
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAG ACACGATTCG CCAGCTGATC CAGCAAGCCC TCGACCAACT GACCGCGGAC GGCACCCTGC CCGCCGGCCT GACCCCGGAC ATCCAGGTGG AGAACACCAA GGACCGTAGC CACGGCGATT TCGCCAGCAA CATCGCCATG ATGCTGGCCA AGCCGGCCGG GATGAAGCCG CGCGACCTGG CCGCCAGGCT GGTCGAGGCA ATCCCCGCCC ACGAGCAACT GGCCAAGGTG GAGATCGCCG GTCCCGGCTT CCTCAACTTC TTCCAGGATC ACGTCTGGCT CGCCGCCAGC CTCGACCGCG CCCTCGCCGA CGAACGTCTC GGCGTGCGCA AGGCAGGCCC GGCGCAGCGC GTGGTGATCG ACCTGTCCTC GCCGAACCTG GCCAAGGAAA TGCACGTCGG CCACCTGCGC TCGACCATCA TCGGCGACGC CGTCGCGCGC GTCCTCGAGT TCCTCGGCGA CACGGTGATC CGGCAGAACC ATGTCGGCGA CTGGGGCACC CAGTTCGGCA TGCTGCTCGC CTACCTGGAA GAGCAACCGG TCGACGCCGA GGCCGAGCTG CACGACCTGG AGGTCTTCTA CCGCGCGGCG AAGAAACGCT TCGACGAGTC TCCCGAATTC GCCGACCGCG CCCGCGAACT GGTAGTCAAG CTGCAGGCCG GCGACCCCGA CTGCCTGCGC CTGTGGACCC GCTTCAACGA GATTTCCCTG TCGCACTGCC AGAAGGTCTA CGACCGTCTC GGCGTGAAGC TGAGCATGGC CGACGTCATG GGCGAGAGCG CCTACAACGA CGACCTCGCC CAGGTGGTCG CCGACCTGAC CGCCAAGGGC CTGCTCACCG AAGACAACGG CGCGTTGTGC GTCTTCCTCG AAGAGTTCAA GAACGCCGAG GGCAATCCGC TCCCGGTGAT CGTGCAGAAG GCCGGCGGCG GCTACCTCTA CGCCACCACC GACCTCGCCG CCATGCGTTA TCGGCACAAC GTGCTGCACG CCGACCGCGT GCTGTACTTC GTCGACCAGC GCCAGGCCCT GCACTTCCAG CAGGTCTTCG AAGTGGCGCG GCGCGCCGGT TTCGTTCCGG CCGGCATGGA GCTGGAGCAC ATGGGCTTCG GCACCATGAA CGGCGCCGAC GGCCGTCCGT TCAAGACCCG CGACGGCGGT ACGGTGAAGC TGATCGACCT GCTGGAAGAG GCCGAGAGCC GCGCCTATGC GCTGGTCAAG GAACGCAACG AGCAGCGCGC CGAGCGTGGC GAGGAGCCGT TCGACGAGGT CCAGCTGCGC GAGATCGGCC GGGTGGTGGG GATCGACTCG GTGAAGTACG CCGACCTGTC CAAGCACCGC ACCAGCGACT ACAGCTTCAA CTTCGAACTG ATGCTGAGCT TCGAAGGCAA CACCGCACCC TACCTGCTCT ACGCCTGCAC CCGCGTGGCC AGCGTCTTCC GCAAGCTCGG CCAGGGCCGC GAGCAACTGG GCGGGAAGAT CGTCCTCGAG CAGCCCCAGG AGCTGGCCCT GGCCGCACAG CTGGCGCAGT TCGGCGACCT GATCAACAAC GTCGCGCTCA AGGGCGTGCC GCACCTGCTC TGCGCCTACC TGTACGAGTT GGCCGGGCTG TTCTCCAGCT TCTACGAGCA TTGCCCGATC CTCACCGCCG AGGACCCGGC GCAGAAGGAC AGCCGCCTGC GTCTCGCCGC GCTGACCGGC CGTACCCTGG AACAGGGCCT GGAGCTGCTC GGTCTGAAGA CTCTGGAACG CATGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT