PsaeA.00163.a

Phenylalanine--tRNA ligase alpha subunit (EC 6.1.1.20) (Phenylalanyl-tRNA synthetase alpha subunit) (PheRS)

CENTER ID: PsaeA.00163.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00163.a.B1.GE38225 full length 1 338
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.2865
PGAT: pseudomonas:PA2740
RefSeq: NP_251430.1
UniProt: Q9I0A3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MENLDALVSQ ALEAVRHTED VNALEQIRVH YLGKKGELTQ VMKTLGDLPA EERPKVGALI NVAKEKVQDV LNARKTELEG AALAARLAAE RIDVTLPGRG QLSGGLHPVT RTLERIEQCF SRIGYEVAEG PEVEDDYHNF EALNIPGHHP ARAMHDTFYF NANMLLRTHT SPVQVRTMES QQPPIRIVCP GRVYRCDSDL THSPMFHQVE GLLVDEGVSF ADLKGTIEEF LRAFFEKQLE VRFRPSFFPF TEPSAEVDIQ CVICSGNGCR VCKQTGWLEV MGCGMVHPNV LRMSNIDPEK FQGFAFGMGA ERLAMLRYGV NDLRLFFDND LRFLGQFR
NT Sequence
atggaaaacc tggatgcgct ggtctctcaa gcattggagg ctgtgcgaca caccgaagac gtcaatgccc tggagcagat ccgggttcac tacctcggca agaaaggcga actgacccag gtgatgaaga ccctgggcga cctgccggcc gaagagcggc cgaaggttgg cgcgctgatc aacgtggcca aggaaaaggt ccaggatgtg ctgaacgcgc gtaagaccga gctggaaggg gccgcgctgg ccgcccggct ggccgcggag cgcatcgatg tcaccctgcc cggtcgtggc cagctgagcg gcggcctgca tccggtcacc cgcacgctgg agcgcatcga gcagtgcttc agccgcatcg gctatgaagt cgccgaaggc ccggaagtcg aagacgacta ccacaacttc gaagcgctca acattccggg ccaccacccg gcgcgtgcca tgcacgacac tttctatttc aatgcgaaca tgctgctgcg tacccacacc tcaccggtgc aggtgcggac catggagtcc cagcagccgc cgatccgcat tgtctgcccg gggcgcgtct accgctgcga ctccgacctg acccactcgc cgatgttcca ccaggtggaa ggcctgttgg tggacgaggg tgtcagcttc gccgacctca agggcaccat cgaggaattc ctccgcgcct tcttcgagaa gcagcttgaa gtgcgcttcc gtccgtcctt cttccccttc accgagccct cggccgaagt ggacattcag tgcgtgatct gcagcggcaa cggctgccgc gtgtgcaagc agaccggctg gctggaagtc atgggttgcg gcatggtgca cccgaacgtg ctgcgcatgt ccaacatcga tcccgagaaa ttccagggct tcgccttcgg catgggcgcc gagcgcctgg ccatgctgcg ctacggggtg aacgacctgc gtctgttctt cgataacgat ctgcgcttcc tcggacaatt ccgctag
Details for PsaeA.00163.a.B1.GE38225
HARVESTED ON: 7/1/2014
SEQUENCED ON: 7/8/2014
EXPECTED MW: 39kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 89
PERCENT COVERAGE: 108
Validated AA Sequence
MAHHHHHHME NLDALVSQAL EAVRHTEDVN ALEQIRVHYL GKKGELTQVM KTLGDLPAEE RPKVGALINV AKEKVQDVLN ARKTELEGAA LAARLAAERI DVTLPGRGQL SGGLHPVTRT LERIEQCFSR IGYEVAEGPE VEDDYHNFEA LNIPGHHPAR AMHDTFYFNA NMLLRTHTSP VQVRTMESQQ PPIRIVCPGR VYRCDSDLTH SPMFHQVEGL LVDEGVSFAD LKGTIEEFLR AFFEKQLEVR FRPSFFPFTE PSAEVDIQCV ICSGNGCRVC KQTGWLEVMG CGMVHPNVLR MSNIDPEKFX ASPSAWAXSA WPCCATGERP ASCSSITIXA SSXXPLVSXS XXXXXXXXXX XXXKKXXXXX VXLCX
Validated NT Sequence
tagaatattt tgtttaactt taagaaggag atataccatg gctcaccacc accaccacca tatggaaaac ctggatgcgc tggtctctca agcattggag gctgtgcgac acaccgaaga cgtcaatgcc ctggagcaga tccgggttca ctacctcggc aagaaaggcg aactgaccca ggtgatgaag accctgggcg acctgccggc cgaagagcgg ccgaaggttg gcgcgctgat caacgtggcc aaggaaaagg tccaggatgt gctgaacgcg cgtaagaccg agctggaagg ggccgcgctg gccgcccggc tggccgcgga gcgcatcgat gtcaccctgc ccggtcgtgg ccagctgagc ggcggcctgc atccggtcac ccgcacgctg gagcgcatcg agcagtgctt cagccgcatc ggctatgaag tcgccgaagg cccggaagtc gaagacgact accacaactt cgaagcgctc aacattccgg gccaccaccc ggcgcgtgcc atgcacgaca ctttctattt caatgcgaac atgctgctgc gtacccacac ctcaccggtg caggtgcgga ccatggagtc ccagcagccg ccgatccgca ttgtctgccc ggggcgcgtc taccgctgcg actccgacct gacccactcg ccgatgttcc accaggtgga aggcctgttg gtggacgagg gtgtcagctt cgccgacctc aagggcacca tcgaggaatt cctccgcgcc ttcttcgaga agcagcttga agtgcgcttc cgtccgtcct tcttcccctt caccgagccc tcggccgaag tggacattca gtgcgtgatc tgcagcggca acggctgccg cgtgtgcaag cagaccggct ggctggaagt catgggttgc ggcatggtgc acccgaacgt gctgcgcatg tccaacatcg atcccgagaa attccnggct tcgccttcgg catgggcgcn gagcgcctgg ccatgctgcg ctacgggtga acgacctgcg tcttgttctt cgataacgat cnncgcntcc tcgganantc cgctagtgag tanatcgnnn nnnnnnnnnn nnannnnnnn agannnnnnn nananaaaaa aangngnnnn tnnnnnngtn gngctntgtn ntnta
Expected Protein Sequence
MAHHHHHHME NLDALVSQAL EAVRHTEDVN ALEQIRVHYL GKKGELTQVM KTLGDLPAEE RPKVGALINV AKEKVQDVLN ARKTELEGAA LAARLAAERI DVTLPGRGQL SGGLHPVTRT LERIEQCFSR IGYEVAEGPE VEDDYHNFEA LNIPGHHPAR AMHDTFYFNA NMLLRTHTSP VQVRTMESQQ PPIRIVCPGR VYRCDSDLTH SPMFHQVEGL LVDEGVSFAD LKGTIEEFLR AFFEKQLEVR FRPSFFPFTE PSAEVDIQCV ICSGNGCRVC KQTGWLEVMG CGMVHPNVLR MSNIDPEKFQ GFAFGMGAER LAMLRYGVND LRLFFDNDLR FLGQFR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa acctggatgc gctggtctct caagcattgg aggctgtgcg acacaccgaa gacgtcaatg ccctggagca gatccgggtt cactacctcg gcaagaaagg cgaactgacc caggtgatga agaccctggg cgacctgccg gccgaagagc ggccgaaggt tggcgcgctg atcaacgtgg ccaaggaaaa ggtccaggat gtgctgaacg cgcgtaagac cgagctggaa ggggccgcgc tggccgcccg gctggccgcg gagcgcatcg atgtcaccct gcccggtcgt ggccagctga gcggcggcct gcatccggtc acccgcacgc tggagcgcat cgagcagtgc ttcagccgca tcggctatga agtcgccgaa ggcccggaag tcgaagacga ctaccacaac ttcgaagcgc tcaacattcc gggccaccac ccggcgcgtg ccatgcacga cactttctat ttcaatgcga acatgctgct gcgtacccac acctcaccgg tgcaggtgcg gaccatggag tcccagcagc cgccgatccg cattgtctgc ccggggcgcg tctaccgctg cgactccgac ctgacccact cgccgatgtt ccaccaggtg gaaggcctgt tggtggacga gggtgtcagc ttcgccgacc tcaagggcac catcgaggaa ttcctccgcg ccttcttcga gaagcagctt gaagtgcgct tccgtccgtc cttcttcccc ttcaccgagc cctcggccga agtggacatt cagtgcgtga tctgcagcgg caacggctgc cgcgtgtgca agcagaccgg ctggctggaa gtcatgggtt gcggcatggt gcacccgaac gtgctgcgca tgtccaacat cgatcccgag aaattccagg gcttcgcctt cggcatgggc gccgagcgcc tggccatgct gcgctacggg gtgaacgacc tgcgtctgtt cttcgataac gatctgcgct tcctcggaca attccgctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at