SSGCID: Target Page

PsaeA.00162.b

DNA gyrase subunit B (EC 5.99.1.3)

CENTER ID:	PsaeA.00162.b
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.00162.b.B1.GE38224	full length		1	806

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.4
PGAT:	pseudomonas:PA0004
RefSeq:	NP_064724.1
UniProt:	Q9I7C2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSENNTYDSS SIKVLKGLDA VRKRPGMYIG DTDDGTGLHH MVFEVVDNSI DEALAGYCSE ISITIHTDES ITVRDNGRGI PVDIHKEEGV SAAEVIMTVL HAGGKFDDNT YKVSGGLHGV GVSVVNALSH ELRLTIRRHN KVWEQVYHHG VPQFPLREVG ETDGSGTEVH FKPSPETFSN IHFSWDILAK RIRELSFLNS GVGILLRDER TGKEELFKYE GGLKAFVEYL NTNKTAVNEV FHFNVQREED GVGVEVALQW NDSFNENLLC FTNNIPQRDG GTHLAGFRSA LTRNLNNYIE AEGLAKKFKI ATTGDDAREG LTAIISVKVP DPKFSSQTKD KLVSSEVKTA VEQEMGKYFA DFLLENPNEA KAVVGKMIDA ARAREAARKA REMTRRKGAL DIAGLPGKLA DCQEKDPALS ELYIVEGDSA GGSAKQGRNR RTQAILPLKG KILNVEKARF DKMLSSQEVG TLITALGCGI GREEYNIDKL RYHNIIIMTD ADVDGSHIRT LLLTFFFRQM PELIERGYIY IAQPPLYKVK RGKQEQYIKD DQAMEEYMTQ SALEDASLHV NEHAPGLSGA ALEKLVNEYR GVIATLKRLS RLYPQELTEH FIYLPTVSVD DLANESAMQG WLEKFQARLT AAEKSGLTYK ASLREDRERH LWLPEVELVA HGLSSYVTFN RDFFASNDYR SVSLLGDQLN SLLEDGAYVQ KGERKRPISA FKDGLDWLMA EGTKRHSIQR YKGLGEMNPE QLWETTMDPN VRRMLKVTIE DAIAADQIFN TLMGDAVEPR RDFIESNALA VSNLDV

NT Sequence

atgagcgaga acaacacgta cgactcttcc agcatcaagg tgctgaaggg gctggatgcc gtacgcaagc gccccggcat gtacatcggc gacaccgacg atggcaccgg tctgcaccac atggtgttcg aggtggtgga taactccatc gacgaagcgc tggccggtta ctgcagcgaa atcagcatca ccatccatac ggatgagtcg atcactgtcc gcgacaatgg acgcggtatt ccggtggata tccacaagga agaaggggtt tctgcggcgg aagtgatcat gaccgtcctc cacgccggcg gcaagttcga cgacaacacc tacaaggtgt ccggcggctt gcacggtgtg ggcgtctcgg tggtgaacgc gctgtcccat gaactacgcc tgaccatccg tcgccacaac aaggtctggg aacaggtcta ccaccacggc gttccgcagt tcccactgcg cgaagtgggc gagaccgatg gctccggcac cgaagttcac ttcaagccgt ccccggagac cttcagcaac atccacttca gttgggacat cctggccaag cgcatccgcg agctgtcctt cctcaactcc ggcgtcggca tcctgctgcg cgacgagcgt accggcaagg aggagctgtt caagtacgaa ggcggtctga aggccttcgt cgagtacctg aacaccaaca agaccgcggt gaacgaggta ttccacttca acgtccagcg tgaagaggac ggcgtgggtg tggaagtcgc cttgcagtgg aacgacagct tcaacgagaa cctgctctgc ttcaccaaca acatcccgca gcgtgacggc ggcacccacc tggccggttt ccgttcggcg ctgacgcgta acctgaacaa ctacatcgag gccgaaggcc tggcgaagaa gttcaagatc gccaccaccg gcgacgatgc ccgcgaaggc ctcaccgcga tcatctcggt gaaggtaccg gacccgaagt tcagctcgca gaccaaggac aagctggtct cctccgaggt gaagactgcg gtggaacagg agatgggcaa gtacttcgcc gacttcctgc tggagaatcc caacgaagcc aaggccgtgg tcggcaagat gatcgacgcc gcccgtgccc gcgaggccgc gcgcaaggcg cgcgagatga cccgccgcaa gggcgcgctg gacatcgccg gcctgcccgg caaactggcc gattgccagg aaaaggaccc ggcgctctcc gaactgtaca tcgtggaggg tgactccgcg ggcggttccg ccaagcaggg ccgcaatcgc cggacccagg cgatcctgcc gctcaagggc aagatcctca acgtcgaaaa ggcgcgcttc gacaagatgc tctcctccca ggaggtcggt acgctgatca ccgccctggg ctgtggcatc ggccgcgagg aatacaacat cgacaagctg cgctaccaca acatcatcat catgaccgat gctgacgtcg acggttcgca catccgcacc ctgctgttga ccttcttctt ccgccagatg cccgagctga tcgagcgtgg ctacatctac atcgcccagc ccccgttgta caaggtcaag cgcggcaagc aggagcagta catcaaggac gaccaggcca tggaagagta catgacccag tcggccctgg aagacgccag cctgcacgtc aacgagcacg ctccgggcct gtccggggcg gcgctggaga aactggtcaa cgagtatcgc ggggtgatcg ccaccctcaa gcgcctgtcg cgcctgtacc cccaggagct gaccgagcac ttcatctacc tgcctaccgt gtcggtggac gacctggcta acgagtcggc catgcagggc tggttggaga agttccaggc gcgcctgacc gccgccgaga agtccggcct gacctacaag gccagcctgc gcgaagaccg cgagcgccac ctgtggctgc ccgaggtgga actggtggcc cacggcctgt ccagctacgt caccttcaac cgtgacttct tcgccagcaa tgactaccgc tcggtgtcgc tgctcggcga ccagctgaac agcctgctgg aagacggcgc ctacgtgcag aagggtgagc gcaagcgccc gatcagcgcc ttcaaggacg gcctggactg gctgatggcc gaaggtacca agcgccacag catccagcga tacaaggggc tgggcgagat gaaccctgag cagctgtggg aaaccaccat ggatccgaac gtccggcgca tgctcaaggt gaccatcgag gatgccatcg ccgccgacca gatcttcaac accctgatgg gcgatgccgt ggagccgcgc cgcgacttca tcgaaagcaa cgcgctggcg gtgtcgaacc tggacgtgtg a

Details for PsaeA.00162.b.B1.GE38224

HARVESTED ON:	7/1/2014
SEQUENCED ON:	7/8/2014
EXPECTED MW:	91kDa
OBSERVED MW:	95kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	93
PERCENT COVERAGE:	39

Validated AA Sequence

MAHHHHHHMS ENNTYDSSSI KVLKGLDAVR KRPGMYIGDT DDGTGLHHMV FEVVDNSIDE ALAGYCSEIS ITIHTDESIT VRDNGRGIPV DIHKEEGVSA AEVIMTVLHA GGKFDDNTYK VSGGLHGVGV SVVNALSHEL RLTIRRXNKV WEQVYHHGVP QFPLREVGXT DGSGTEVHXX PSPETFSNIH FSWDILAKRI RELSFLNSGV GILLRDERTG KEELFKYEGG LKAFVEYLNT NKTAVNEVFH FNVQREEDGV GVEVALQWND SFNENLLCFT NNIPQRDGGT HLAGFRSALX RXLXXTSRXX GLXXKFXIXX TXXXC

Validated NT Sequence

atggctcacc accaccacca ccatatgagc gagaacaaca cgtacgactc ttccagcatc aaggtgctga aggggctgga tgccgtacgc aagcgccccg gcatgtacat cggcgacacc gacgatggca ccggtctgca ccacatggtg ttcgaggtgg tggataactc catcgacgaa gcgctggccg gttactgcag cgaaatcagc atcaccatcc atacggatga gtcgatcact gtccgcgaca atggacgcgg tattccggtg gatatccaca aggaagaagg ggtttctgcg gcggaagtga tcatgaccgt cctccacgcc ggcggcaagt tcgacgacaa cacctacaag gtgtccggcg gcttgcacgg tgtgggcgtc tcggtggtga acgcgctgtc ccatgaacta cgcctgacca tccgtcgccn caacaaggtc tgggaacagg tctaccacca cggcgttccg cagttcccac tgcgcgaagt gggcganacc gatggctccg gcaccgaagt tcacnnnnng ccgtccccgg agaccttcag caacatccac ttcagttggg acatcctggc caagcgcatc cgcgagctgt ccttcctcaa ctccggcgtc ggcatcctgc tgcgcgacga gcgtaccggc aaggaggagc tgttcaagta cgaaggcggt ctgaaggcct tcgtcgagta cctgaacacc aacaagaccg cggtgaacga ggtattccac ttcaacgtcc agcgtgaaga ggacggcgtg ggtgtggaag tcgccttgca gtggaacgac agcttcaacg agaacctgct ctgcttcacc aacaacatcc cgcagcgtga cggcggcacc cacctggccg gtttccgttc ggcgctgang cgtaanctga anantacatc gaggncnnan ggnctggnna nnaagttcnn natcnnnnnc accngnnnan natgccc

Expected Protein Sequence

MAHHHHHHMS ENNTYDSSSI KVLKGLDAVR KRPGMYIGDT DDGTGLHHMV FEVVDNSIDE ALAGYCSEIS ITIHTDESIT VRDNGRGIPV DIHKEEGVSA AEVIMTVLHA GGKFDDNTYK VSGGLHGVGV SVVNALSHEL RLTIRRHNKV WEQVYHHGVP QFPLREVGET DGSGTEVHFK PSPETFSNIH FSWDILAKRI RELSFLNSGV GILLRDERTG KEELFKYEGG LKAFVEYLNT NKTAVNEVFH FNVQREEDGV GVEVALQWND SFNENLLCFT NNIPQRDGGT HLAGFRSALT RNLNNYIEAE GLAKKFKIAT TGDDAREGLT AIISVKVPDP KFSSQTKDKL VSSEVKTAVE QEMGKYFADF LLENPNEAKA VVGKMIDAAR AREAARKARE MTRRKGALDI AGLPGKLADC QEKDPALSEL YIVEGDSAGG SAKQGRNRRT QAILPLKGKI LNVEKARFDK MLSSQEVGTL ITALGCGIGR EEYNIDKLRY HNIIIMTDAD VDGSHIRTLL LTFFFRQMPE LIERGYIYIA QPPLYKVKRG KQEQYIKDDQ AMEEYMTQSA LEDASLHVNE HAPGLSGAAL EKLVNEYRGV IATLKRLSRL YPQELTEHFI YLPTVSVDDL ANESAMQGWL EKFQARLTAA EKSGLTYKAS LREDRERHLW LPEVELVAHG LSSYVTFNRD FFASNDYRSV SLLGDQLNSL LEDGAYVQKG ERKRPISAFK DGLDWLMAEG TKRHSIQRYK GLGEMNPEQL WETTMDPNVR RMLKVTIEDA IAADQIFNTL MGDAVEPRRD FIESNALAVS NLDV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg agaacaacac gtacgactct tccagcatca aggtgctgaa ggggctggat gccgtacgca agcgccccgg catgtacatc ggcgacaccg acgatggcac cggtctgcac cacatggtgt tcgaggtggt ggataactcc atcgacgaag cgctggccgg ttactgcagc gaaatcagca tcaccatcca tacggatgag tcgatcactg tccgcgacaa tggacgcggt attccggtgg atatccacaa ggaagaaggg gtttctgcgg cggaagtgat catgaccgtc ctccacgccg gcggcaagtt cgacgacaac acctacaagg tgtccggcgg cttgcacggt gtgggcgtct cggtggtgaa cgcgctgtcc catgaactac gcctgaccat ccgtcgccac aacaaggtct gggaacaggt ctaccaccac ggcgttccgc agttcccact gcgcgaagtg ggcgagaccg atggctccgg caccgaagtt cacttcaagc cgtccccgga gaccttcagc aacatccact tcagttggga catcctggcc aagcgcatcc gcgagctgtc cttcctcaac tccggcgtcg gcatcctgct gcgcgacgag cgtaccggca aggaggagct gttcaagtac gaaggcggtc tgaaggcctt cgtcgagtac ctgaacacca acaagaccgc ggtgaacgag gtattccact tcaacgtcca gcgtgaagag gacggcgtgg gtgtggaagt cgccttgcag tggaacgaca gcttcaacga gaacctgctc tgcttcacca acaacatccc gcagcgtgac ggcggcaccc acctggccgg tttccgttcg gcgctgacgc gtaacctgaa caactacatc gaggccgaag gcctggcgaa gaagttcaag atcgccacca ccggcgacga tgcccgcgaa ggcctcaccg cgatcatctc ggtgaaggta ccggacccga agttcagctc gcagaccaag gacaagctgg tctcctccga ggtgaagact gcggtggaac aggagatggg caagtacttc gccgacttcc tgctggagaa tcccaacgaa gccaaggccg tggtcggcaa gatgatcgac gccgcccgtg cccgcgaggc cgcgcgcaag gcgcgcgaga tgacccgccg caagggcgcg ctggacatcg ccggcctgcc cggcaaactg gccgattgcc aggaaaagga cccggcgctc tccgaactgt acatcgtgga gggtgactcc gcgggcggtt ccgccaagca gggccgcaat cgccggaccc aggcgatcct gccgctcaag ggcaagatcc tcaacgtcga aaaggcgcgc ttcgacaaga tgctctcctc ccaggaggtc ggtacgctga tcaccgccct gggctgtggc atcggccgcg aggaatacaa catcgacaag ctgcgctacc acaacatcat catcatgacc gatgctgacg tcgacggttc gcacatccgc accctgctgt tgaccttctt cttccgccag atgcccgagc tgatcgagcg tggctacatc tacatcgccc agcccccgtt gtacaaggtc aagcgcggca agcaggagca gtacatcaag gacgaccagg ccatggaaga gtacatgacc cagtcggccc tggaagacgc cagcctgcac gtcaacgagc acgctccggg cctgtccggg gcggcgctgg agaaactggt caacgagtat cgcggggtga tcgccaccct caagcgcctg tcgcgcctgt acccccagga gctgaccgag cacttcatct acctgcctac cgtgtcggtg gacgacctgg ctaacgagtc ggccatgcag ggctggttgg agaagttcca ggcgcgcctg accgccgccg agaagtccgg cctgacctac aaggccagcc tgcgcgaaga ccgcgagcgc cacctgtggc tgcccgaggt ggaactggtg gcccacggcc tgtccagcta cgtcaccttc aaccgtgact tcttcgccag caatgactac cgctcggtgt cgctgctcgg cgaccagctg aacagcctgc tggaagacgg cgcctacgtg cagaagggtg agcgcaagcg cccgatcagc gccttcaagg acggcctgga ctggctgatg gccgaaggta ccaagcgcca cagcatccag cgatacaagg ggctgggcga gatgaaccct gagcagctgt gggaaaccac catggatccg aacgtccggc gcatgctcaa ggtgaccatc gaggatgcca tcgccgccga ccagatcttc aacaccctga tgggcgatgc cgtggagccg cgccgcgact tcatcgaaag caacgcgctg gcggtgtcga acctggacgt gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat