SSGCID: Target Page

PsaeA.00157.b

DNA gyrase subunit A (EC 5.99.1.3)

CENTER ID:	PsaeA.00157.b
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.00157.b.B2.GE38211	polypeptide domain		1	507
PsaeA.00157.b.B3.GE38212	polypeptide repeats domain		540	882

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.00157.b.B3.PS02193	polypeptide repeats domain		540	882

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.3311
PGAT:	pseudomonas:PA3168
RefSeq:	NP_251858.1
UniProt:	P48372

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGELAKEILP VNIEDELKQS YLDYAMSVIV GRALPDARDG LKPVHRRVLY AMSELGNDWN KPYKKSARVV GDVIGKYHPH GDTAVYDTIV RMAQPFSLRY MLVDGQGNFG SVDGDNAAAM RYTEVRMAKL AHELLADLEK ETVDWVPNYD GTEQIPAVMP TKIPNLLVNG SSGIAVGMAT NIPPHNLGEV IDGCLALMDN PDLTVDELMQ YIPGPDFPTA GIINGRAGII EAYRTGRGRI YIRARAVVEE MEKGGGREQI IITELPYQLN KARLIEKIAE LVKEKKIEGI SELRDESDKD GMRVVIELRR GEVGEVVLNN LYAQTQLQSV FGINVVALVD GQPRTLNLKD MLEVFVRHRR EVVTRRTVYE LRKARERGHI LEGQAVALSN IDPVIELIKS SPTPAEAKER LIATAWESSA VEAMVERAGA DACRPEDLDP QYGLRDGKYY LSPEQAQAIL ELRLHRLTGL EHEKLLSEYQ EILNLIGELI RILTNPARLM EVIREELEAV KAEFGDARRT EIVASQVDLT IADLITEEDR VVTISHGGYA KSQPLAAYQA QRRGGKGKSA TGMKDEDYIE HLLVANSHAT LLLFSSKGKV YWLRTFEIPE ASRTARGRPL VNLLPLDEGE RITAMLQIDL EALQQNGGAD DDLDEAEGAV LEGEVVEAAE VEEVEGETAE LVAEPTGAYI FMATAFGTVK KTPLVQFSRP RSSGLIALKL EEGDTLIAAA ITDGAKEVML FSSAGKVIRF AESVVRIMGR NARGVRGMRL GKGQQLISML IPESGAQILT ASERGFGKRT PLSKFPRRGR GGQGVIAMVT NERNGALIAA VQVQEGEEIM LISDQGTLVR TRVDEVSLSG RNTQGVTLIK LASDEVLVGL ERVQEPSGGD DEDLPEGEEA AESLGESAES ESEPAAEAEG NEE

NT Sequence

atgggcgaac tggccaaaga aattctcccg gtcaatatcg aagacgagct gaaacagtcc tatctcgact acgcgatgag cgtgatcgtc gggcgggccc tgccggatgc acgtgacggc ctgaagccgg tgcaccgccg tgtgctttat gccatgagcg agctgggcaa cgactggaac aagccctaca agaaatccgc ccgtgtggtc ggcgacgtga tcggtaagta ccacccgcac ggcgacaccg cggtctacga caccatcgtg cgcatggcgc agccgttctc gctgcgctac atgctggtag acggccaggg caacttcggt tcggtggacg gcgacaacgc cgcagccatg cgatacaccg aagtgcgcat ggccaagctg gcccacgaac tgctggcgga cctggaaaag gaaaccgtcg actgggtgcc caactacgat ggcaccgagc agatcccggc ggtcatgccg accaagattc ccaacctgct ggtcaacggt tccagcggta tcgccgtggg catggcgacc aacatcccgc cgcacaacct cggcgaagtg atcgacggct gcctggcgct gatggacaac cccgacctga ccgtcgatga gctgatgcag tacatccccg gtccggactt ccccaccgcc ggcatcatca acggccgcgc cgggatcatc gaggcctacc gcaccggtcg cgggcgcatc tacatccgtg cccgcgccgt cgtcgaggag atggagaagg gcggcggtcg cgagcagatt atcatcaccg agctgccgta ccagttgaac aaggcgcggt tgatcgagaa gatcgccgag ctggtgaaag agaagaagat cgagggtatt tccgagctgc gcgacgagtc tgacaaggac ggcatgcgcg tggtcatcga gctgcgtcgc ggcgaggtgg gcgaggtggt cctcaacaac ctctatgccc agacccagct gcagagcgtg ttcggcatca acgtggtggc cctggtcgac ggccagccgc gcacgctgaa cctgaaggac atgctcgagg tgttcgtccg ccaccgccgc gaagtggtga cccggcgtac cgtctacgag ctgcgcaagg cccgcgagcg cgggcacatc ctggaaggcc aggcggtcgc cctgtcgaac atcgacccgg tgatcgagct gatcaagagt tcgccgaccc cggccgaggc caaggaacgc ctgatcgcca ctgcctggga gtccagcgcg gtggaagcga tggtcgagcg tgccggcgcc gacgcctgtc gtccggaaga cctggatccg cagtacggcc tgcgcgacgg caagtactac ctgtcgccgg agcaggccca ggcgatcctc gagctgcgcc tgcatcgcct gaccggcctg gagcacgaga agctgctctc cgaataccag gaaatcctca acctgatcgg cgagctgatc cgcatcctga ccaacccggc gcgcctgatg gaggtgatcc gtgaggaact ggaagcggtc aaggccgaat tcggcgatgc tcgccgcacc gagatcgtgg cttcccaggt cgacctgacc atcgccgacc tgatcaccga ggaagaccgc gtggtgacca tctcgcacgg cggctacgcc aagtcccagc cgctggccgc ctaccaggcg cagcgtcgcg gcggcaaagg caagtccgcc accgggatga aggacgagga ctacatcgaa cacctgctgg tggccaacag ccatgcgacc ctcctgctgt tctccagcaa gggcaaggtc tactggctgc gtaccttcga gattccggaa gcctcgcgta ccgcgcgtgg ccggccgctg gtgaacctgc tgccgctgga tgagggcgag cggatcaccg cgatgttgca gatcgacctg gaggcgctgc agcagaacgg tggcgccgat gacgacctcg acgaagccga aggcgcggtg ctcgagggcg aggtggtcga ggccgccgag gtcgaggaag tcgagggcga gaccgccgag ctggtggccg agccgaccgg cgcctacatc ttcatggcca ccgccttcgg taccgtgaag aagaccccgc tggtgcagtt cagccgtccg cgcagcagcg gcctgatcgc gctcaagctg gaagagggcg acaccctgat cgccgccgcg atcaccgatg gcgccaagga agtcatgctg ttctccagcg ccggcaaggt gatccgcttc gccgagagcg tggtgcgcat catgggccgc aacgcccgcg gcgtacgtgg catgcgcctg ggcaaggggc agcagctgat ctccatgctg attccggagt ccggggcgca gatcctcacc gcctccgagc gcggcttcgg caagcgtacc ccgctgagca agttcccgcg tcgcggccgc ggcggccagg gggtgatcgc catggtcacc aacgagcgca acggcgcgct gatcgccgcg gtacaggtcc aggaaggcga ggagatcatg ctgatttccg accagggcac cctggtgcgg acgcgtgtcg acgaagtctc cctgtccggc cgcaataccc agggcgtaac cctgatcaag ctcgccagcg acgaggtact ggtcggtctg gagcgtgtcc aggagccgtc gggcggagat gacgaggacc tgcccgaggg cgaggaagct gccgaatctc tgggcgagtc ggccgagtcc gagtccgagc ccgcggcgga agcggaaggc aacgaagagt aa

Details for PsaeA.00157.b.B2.GE38211

HARVESTED ON:	6/11/2014
SEQUENCED ON:	6/12/2014
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	53

Validated AA Sequence

Validated NT Sequence

tcnntagnnt nttttgttta actttaagaa ggagatatac catggctcac caccaccacc accatatggg cgaactggcc aaagaaattc tcccggtcaa tatcgaagac gagctgaaac agtcctatct cgactacgcg atgagcgtga tcgtcgggcg ggccctgccg gatgcacgtg acggcctgaa gccggtgcac cgccgtgtgc tttatgccat gagcgagctg ggcaacgact ggaacaagcc ctacaagaaa tccgcccgtg tggtcggcga cgtgatcggt aagtaccacc cgcacggcga caccgcggtc tacgacacca tcgtgcgcat ggcgcagccg ttctcgctgc gctacatgct ggtagacggc cagggcaact tcggttcggt ggacggcgac aacgccgcag ccatgcgata caccgaagtg cgcatggcca agctggccca cgaactgctg gcggacctgg aaaaggaaac cgtcgactgg gtgcccaact acgatggcac cgagcagatc ccggcggtca tgccgaccaa gattcccaac ctgctggtca acggttccag cggtatcgcc gtgggcatgg cgaccaacat cccgccgcac aacctcggcg aagtgatcga cggctgcctg gcgctgatgg acaaccccga cctgaccgtc gatgagctga tgcagtacat ccccggtccg gacttcccca ccgccggcat catcaacggc cgcgccggga tcatcgaggc ctaccgcacc ggtcgcgggc gcatctacat ccgtgcccgc gccgtcgtcg aggagatgga gaagggcggc ggtcgcgagc agatatcatc accgagctgc cgtaccagtt gaacaaggcg cggttgatcg agaagatcgc cgagctggtg aaagagaaga agatcgaggg tatttccgag ctgcgcgacg agtctgacaa ggacggcatg cgcgtggtca tcgagctgcg tcgcggcgag gtgggcgagg tggtcctcaa caacctctat gcccagaccc agctgcagag cgtgtcggca tcacgtgnng ccctggncga cggcagcncg cacgctgann gnnncatgct cgagngtncg ttccnccnnn nccncnanng ngaccnggcn tncngtctnn nnanctnnnn nnccncgnnn nnngnnnatc tgnnngnnng gcgntnnccn nncnaacanc tcnacccggn nnnaanngnn nnnnatnnna anangatnnn tcnc

Expected Protein Sequence

MAHHHHHHMG ELAKEILPVN IEDELKQSYL DYAMSVIVGR ALPDARDGLK PVHRRVLYAM SELGNDWNKP YKKSARVVGD VIGKYHPHGD TAVYDTIVRM AQPFSLRYML VDGQGNFGSV DGDNAAAMRY TEVRMAKLAH ELLADLEKET VDWVPNYDGT EQIPAVMPTK IPNLLVNGSS GIAVGMATNI PPHNLGEVID GCLALMDNPD LTVDELMQYI PGPDFPTAGI INGRAGIIEA YRTGRGRIYI RARAVVEEME KGGGREQIII TELPYQLNKA RLIEKIAELV KEKKIEGISE LRDESDKDGM RVVIELRRGE VGEVVLNNLY AQTQLQSVFG INVVALVDGQ PRTLNLKDML EVFVRHRREV VTRRTVYELR KARERGHILE GQAVALSNID PVIELIKSSP TPAEAKERLI ATAWESSAVE AMVERAGADA CRPEDLDPQY GLRDGKYYLS PEQAQAILEL RLHRLTGLEH EKLLSEYQEI LNLIGELIRI LTNPARLMEV IREEL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcg aactggccaa agaaattctc ccggtcaata tcgaagacga gctgaaacag tcctatctcg actacgcgat gagcgtgatc gtcgggcggg ccctgccgga tgcacgtgac ggcctgaagc cggtgcaccg ccgtgtgctt tatgccatga gcgagctggg caacgactgg aacaagccct acaagaaatc cgcccgtgtg gtcggcgacg tgatcggtaa gtaccacccg cacggcgaca ccgcggtcta cgacaccatc gtgcgcatgg cgcagccgtt ctcgctgcgc tacatgctgg tagacggcca gggcaacttc ggttcggtgg acggcgacaa cgccgcagcc atgcgataca ccgaagtgcg catggccaag ctggcccacg aactgctggc ggacctggaa aaggaaaccg tcgactgggt gcccaactac gatggcaccg agcagatccc ggcggtcatg ccgaccaaga ttcccaacct gctggtcaac ggttccagcg gtatcgccgt gggcatggcg accaacatcc cgccgcacaa cctcggcgaa gtgatcgacg gctgcctggc gctgatggac aaccccgacc tgaccgtcga tgagctgatg cagtacatcc ccggtccgga cttccccacc gccggcatca tcaacggccg cgccgggatc atcgaggcct accgcaccgg tcgcgggcgc atctacatcc gtgcccgcgc cgtcgtcgag gagatggaga agggcggcgg tcgcgagcag attatcatca ccgagctgcc gtaccagttg aacaaggcgc ggttgatcga gaagatcgcc gagctggtga aagagaagaa gatcgagggt atttccgagc tgcgcgacga gtctgacaag gacggcatgc gcgtggtcat cgagctgcgt cgcggcgagg tgggcgaggt ggtcctcaac aacctctatg cccagaccca gctgcagagc gtgttcggca tcaacgtggt ggccctggtc gacggccagc cgcgcacgct gaacctgaag gacatgctcg aggtgttcgt ccgccaccgc cgcgaagtgg tgacccggcg taccgtctac gagctgcgca aggcccgcga gcgcgggcac atcctggaag gccaggcggt cgccctgtcg aacatcgacc cggtgatcga gctgatcaag agttcgccga ccccggccga ggccaaggaa cgcctgatcg ccactgcctg ggagtccagc gcggtggaag cgatggtcga gcgtgccggc gccgacgcct gtcgtccgga agacctggat ccgcagtacg gcctgcgcga cggcaagtac tacctgtcgc cggagcaggc ccaggcgatc ctcgagctgc gcctgcatcg cctgaccggc ctggagcacg agaagctgct ctccgaatac caggaaatcc tcaacctgat cggcgagctg atccgcatcc tgaccaaccc ggcgcgcctg atggaggtga tccgtgagga actgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for PsaeA.00157.b.B3.GE38212

HARVESTED ON:	6/11/2014
SEQUENCED ON:	6/12/2014
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MRVVTISHGG YAKSQPLAAY QAQRRGGKGK SATGMKDEDY IEHLLVANSH ATLLLFSSKG KVYWLRTFEI PEASRTARGR PLVNLLPLDE GERITAMLQI DLEALQQNGG ADDDLDEAEG AVLEGEVVEA AEVEEVEGET AELVAEPTGA YIFMATAFGT VKKTPLVQFS RPRSSGLIAL KLEEGDTLIA AAITDGAKEV MLFSSAGKVI RFAESVVRIM GRNARGVRGM RLGKGQQLIS MLIPESGAQI LTASERGFGK RTPLSKFPRR GRGGQGVIAM VTNERNGALI AAVQVQEGEE IMLISDQGTL VRTRVDEVSL SGRNTQGVTL IKLASDEVLV GLER

Validated NT Sequence

tcttactcac ttaacgctcc agaccgacca gtacctcgtc gctggcgagc ttgatcaggg ttacgccctg ggtattgcgg ccggacaggg agacttcgtc gacacgcgtc cgcaccaggg tgccctggtc ggaaatcagc atgatctcct cgccttcctg gacctgtacc gcggcgatca gcgcgccgtt gcgctcgttg gtgaccatgg cgatcacccc ctggccgccg cggccgcgac gcgggaactt gctcagcggg gtacgcttgc cgaagccgcg ctcggaggcg gtgaggatct gcgccccgga ctccggaatc agcatggaga tcagctgctg ccccttgccc aggcgcatgc cacgtacgcc gcgggcgttg cggcccatga tgcgcaccac gctctcggcg aagcggatca ccttgccggc gctggagaac agcatgactt ccttggcgcc atcggtgatc gcggcggcga tcagggtgtc gccctcttcc agcttgagcg cgatcaggcc gctgctgcgc ggacggctga actgcaccag cggggtcttc ttcacggtac cgaaggcggt ggccatgaag atgtaggcgc cggtcggctc ggccaccagc tcggcggtct cgccctcgac ttcctcgacc tcggcggcct cgaccacctc gccctcgagc accgcgcctt cggcttcgtc gaggtcgtca tcggcgccac cgttctgctg cagcgcctcc aggtcgatct gcaacatcgc ggtgatccgc tcgccctcat ccagcggcag caggttcacc agcggccggc cacgcgcggt acgcgaggct tccggaatct cgaaggtacg cagccagtag accttgccct tgctggagaa cagcaggagg gtcgcatggc tgttggccac cagcaggtgt tcgatgtagt cctcgtcctt catcccggtg gcggacttgc ctttgccgcc gcgacgctgc gcctggtagg cggccagcgg ctgggacttg gcgtagccgc cgtgcgagat ggtcaccacg cgcatatg

Expected Protein Sequence

MAHHHHHHRV VTISHGGYAK SQPLAAYQAQ RRGGKGKSAT GMKDEDYIEH LLVANSHATL LLFSSKGKVY WLRTFEIPEA SRTARGRPLV NLLPLDEGER ITAMLQIDLE ALQQNGGADD DLDEAEGAVL EGEVVEAAEV EEVEGETAEL VAEPTGAYIF MATAFGTVKK TPLVQFSRPR SSGLIALKLE EGDTLIAAAI TDGAKEVMLF SSAGKVIRFA ESVVRIMGRN ARGVRGMRLG KGQQLISMLI PESGAQILTA SERGFGKRTP LSKFPRRGRG GQGVIAMVTN ERNGALIAAV QVQEGEEIML ISDQGTLVRT RVDEVSLSGR NTQGVTLIKL ASDEVLVGLE R

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgcgtgg tgaccatctc gcacggcggc tacgccaagt cccagccgct ggccgcctac caggcgcagc gtcgcggcgg caaaggcaag tccgccaccg ggatgaagga cgaggactac atcgaacacc tgctggtggc caacagccat gcgaccctcc tgctgttctc cagcaagggc aaggtctact ggctgcgtac cttcgagatt ccggaagcct cgcgtaccgc gcgtggccgg ccgctggtga acctgctgcc gctggatgag ggcgagcgga tcaccgcgat gttgcagatc gacctggagg cgctgcagca gaacggtggc gccgatgacg acctcgacga agccgaaggc gcggtgctcg agggcgaggt ggtcgaggcc gccgaggtcg aggaagtcga gggcgagacc gccgagctgg tggccgagcc gaccggcgcc tacatcttca tggccaccgc cttcggtacc gtgaagaaga ccccgctggt gcagttcagc cgtccgcgca gcagcggcct gatcgcgctc aagctggaag agggcgacac cctgatcgcc gccgcgatca ccgatggcgc caaggaagtc atgctgttct ccagcgccgg caaggtgatc cgcttcgccg agagcgtggt gcgcatcatg ggccgcaacg cccgcggcgt acgtggcatg cgcctgggca aggggcagca gctgatctcc atgctgattc cggagtccgg ggcgcagatc ctcaccgcct ccgagcgcgg cttcggcaag cgtaccccgc tgagcaagtt cccgcgtcgc ggccgcggcg gccagggggt gatcgccatg gtcaccaacg agcgcaacgg cgcgctgatc gccgcggtac aggtccagga aggcgaggag atcatgctga tttccgacca gggcaccctg gtgcggacgc gtgtcgacga agtctccctg tccggccgca atacccaggg cgtaaccctg atcaagctcg ccagcgacga ggtactggtc ggtctggagc gttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for PsaeA.00157.b.B3.PS02193

PURIFICATION DATe:	10/23/2014
CONCENTRATION:	56mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCGCGTGG TGACCATCTC GCACGGCGGC TACGCCAAGT CCCAGCCGCT GGCCGCCTAC CAGGCGCAGC GTCGCGGCGG CAAAGGCAAG TCCGCCACCG GGATGAAGGA CGAGGACTAC ATCGAACACC TGCTGGTGGC CAACAGCCAT GCGACCCTCC TGCTGTTCTC CAGCAAGGGC AAGGTCTACT GGCTGCGTAC CTTCGAGATT CCGGAAGCCT CGCGTACCGC GCGTGGCCGG CCGCTGGTGA ACCTGCTGCC GCTGGATGAG GGCGAGCGGA TCACCGCGAT GTTGCAGATC GACCTGGAGG CGCTGCAGCA GAACGGTGGC GCCGATGACG ACCTCGACGA AGCCGAAGGC GCGGTGCTCG AGGGCGAGGT GGTCGAGGCC GCCGAGGTCG AGGAAGTCGA GGGCGAGACC GCCGAGCTGG TGGCCGAGCC GACCGGCGCC TACATCTTCA TGGCCACCGC CTTCGGTACC GTGAAGAAGA CCCCGCTGGT GCAGTTCAGC CGTCCGCGCA GCAGCGGCCT GATCGCGCTC AAGCTGGAAG AGGGCGACAC CCTGATCGCC GCCGCGATCA CCGATGGCGC CAAGGAAGTC ATGCTGTTCT CCAGCGCCGG CAAGGTGATC CGCTTCGCCG AGAGCGTGGT GCGCATCATG GGCCGCAACG CCCGCGGCGT ACGTGGCATG CGCCTGGGCA AGGGGCAGCA GCTGATCTCC ATGCTGATTC CGGAGTCCGG GGCGCAGATC CTCACCGCCT CCGAGCGCGG CTTCGGCAAG CGTACCCCGC TGAGCAAGTT CCCGCGTCGC GGCCGCGGCG GCCAGGGGGT GATCGCCATG GTCACCAACG AGCGCAACGG CGCGCTGATC GCCGCGGTAC AGGTCCAGGA AGGCGAGGAG ATCATGCTGA TTTCCGACCA GGGCACCCTG GTGCGGACGC GTGTCGACGA AGTCTCCCTG TCCGGCCGCA ATACCCAGGG CGTAACCCTG ATCAAGCTCG CCAGCGACGA GGTACTGGTC GGTCTGGAGC GTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT