SSGCID: Target Page

PsaeA.00156.a

Threonine--tRNA ligase (EC 6.1.1.3) (Threonyl-tRNA synthetase) (ThrRS)

CENTER ID:	PsaeA.00156.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.00156.a.B1.GE38209	full length		1	640
PsaeA.00156.a.B2.GE40429	truncation 1		1	240

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.2869
PGAT:	pseudomonas:PA2744
RefSeq:	NP_251434.1
UniProt:	Q9I099

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPIITLPDGS QRSFDHPVSV AEVAQSIGAG LAKATLAGKV DGRLVDACDT IDRDATLQII TPKDEEGLEI IRHSCAHLVG HAVKQLYPTA KMVIGPVIEE GFYYDIFFER PFTPEDMAAI QQRMRELIDK DYDVIKKMTP RAEVIELFKS RGEDYKLRLI DDMPDEKAMG LYFHEEYVDM CRGPHVPNTR FLKAFQLTKI SGAYWRGDSK NEQLQRIYGT AWADKKQLAA YIQRIEEAEK RDHRRIGKQL DLFHLQEEAP GMVFWHPNGW SVYQVLEQYM RKVQRDHGYV EVRTPQVVDR ILWERSGHWS NYAENMFTTS SESRDYAVKP MNCPCHVQIF NQGLKSYRDL PLRLAEFGAC HRNEPSGALH GIMRVRGFTQ DDAHIFCTEE QVKKEAADFI KLTLQVYRDF GFTDIAMKLS TRPAKRVGSD ELWDRAEGAL ADALNESGLA WEYQPGEGAF YGPKIEFTLK DCLGRNWQCG TLQYDPNLPE RLDASYIAED NNRKRPVMLH RAILGSFERF IGMLIEHYAG AFPAWLAPTQ AVVMNITDKQ ADFAAEVVRI LGESGFRAKS DLRNEKIGFK IREHTLLKVP YLLVIGDREV ESKAVAVRTR EGEDLGSMPV TQFAELLAQA VSRRGRQDSE

NT Sequence

atgcccatca ttactcttcc cgacggcagt caacgttcct tcgatcaccc ggtctccgtg gccgaggtgg cccaatccat cggcgcgggc ctggccaagg cgaccctcgc cggcaaggtc gacggccgcc tggtcgacgc ctgcgacacc atcgatcgcg acgcgaccct gcagatcatc acgcccaagg acgaggaagg actggagatc atccgccact cctgcgccca cctggtcggc catgcggtca agcagctcta tccgaccgcg aagatggtca tcggcccggt gatcgaggaa ggcttctact acgacatttt cttcgagcgc cccttcaccc ccgaggacat ggcggcgatc cagcagcgca tgcgcgagct gatcgacaag gactacgacg tgatcaagaa gatgaccccg cgcgccgagg tcatcgagct gttcaagtcc cgtggcgaag actacaagct gcgcctgatc gacgacatgc cggacgagaa ggccatgggc ctgtacttcc atgaggagta cgtggacatg tgccgcggcc cgcatgtgcc gaacacccgc ttcctcaagg cgttccaact gaccaagatt tccggcgcct actggcgcgg cgactcgaag aacgagcagt tgcaacgcat ctacggcacc gcctgggccg acaagaagca actggcggcc tacatccagc gcatcgaaga ggccgagaag cgcgaccatc gccgcatcgg caagcagctc gacctgttcc acctgcagga agaagcgccg ggcatggtgt tctggcaccc gaatggctgg agcgtctacc aggtgctcga gcagtacatg cgcaaggtcc agcgcgacca tggctatgtc gaagtgcgta ccccgcaggt ggtcgaccgc atcctctggg agcgttcggg ccactggtcg aactacgccg agaacatgtt caccacctcc tcggaaagtc gcgactacgc ggtcaagccg atgaactgcc cgtgccacgt gcagatcttc aaccagggcc tgaagtccta ccgcgacctg ccgctgcgcc tcgccgagtt cggcgcctgc caccgcaacg agccgtccgg cgcgctgcac gggatcatgc gcgtacgcgg ctttacccag gacgacgcgc atatcttctg caccgaagag caggtgaaga aggaagcggc cgatttcatc aagctgacct tgcaggtcta ccgcgacttc ggtttcaccg acatcgccat gaagctgtcg acccgtccgg ccaagcgcgt cggttccgac gagctgtggg atcgcgccga aggcgcgctg gccgatgcgc tgaacgaatc cggcctggcc tgggaatacc agccgggcga gggcgcgttc tacgggccga agatcgagtt caccctgaag gactgcctcg gccgtaactg gcagtgcggc accctgcagt acgacccgaa cctgccggag cgcctggacg ccagctatat cgccgaggac aacaaccgta agcgtccggt gatgctgcac cgtgcgatcc tcgggtcctt cgagcgcttc atcggcatgc tcatcgagca ctacgccgga gccttcccgg cctggctggc gccgacccag gcagtggtga tgaacatcac cgacaagcag gccgatttcg ccgccgaggt ggtgcggatc ctcggggaaa gcggattccg tgccaagtcc gacttgagaa acgagaagat cggctttaaa atccgcgagc atactttgct caaggttccc tatctcttgg ttattggaga tcgggaagtt gaatcgaagg ccgtcgcggt gcgtacgcgc gaaggggaag acctgggctc catgcccgtc acccagttcg ctgagctgtt ggcacaggcg gtttcccggc gtggtcgcca agactcggag taa

Details for PsaeA.00156.a.B1.GE38209

HARVESTED ON:	6/11/2014
SEQUENCED ON:	6/12/2014
EXPECTED MW:	74kDa
OBSERVED MW:	74kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	93
PERCENT COVERAGE:	52

Validated AA Sequence

Validated NT Sequence

annnncncta gaataatttt gtttacttta agaaggagat ataccatggc tcaccaccac caccaccata tgcccatcat tactcttccc gacggcagtc aacgttcctt cgatcacccg gtctccgtgg ccgaggtggc ccaatccatc ggcgcgggcc tggccaaggc gaccctcgcc ggcaaggtcg acggccgcct ggtcgacgcc tgcgacacca tcgatcgcga cgcgaccctg cagatcatca cgcccaagga cgaggaagga ctggagatca tccgccactc ctgcgcccac ctggtcggcc atgcggtcaa gcagctctat ccgaccgcga agatggtcat cggcccggtg atcgaggaag gcttctacta cgacattttc ttcgagcgcc ccttcacccc cgaggacatg gcggcgatcc agcagcgcat gcgcgagctg atcgacaagg actacgacgt gatcaagaag atgaccccgc gcgccgaggt catcgagctg ttcaagtccc gtggcgaaga ctacaagctg cgcctgatcg acgacatgcc ggacgagaag gccatgggcc tgtacttcca tgaggagtac gtggacatgt gccgcggccc gcatgtgccg aacacccgct tcctcaaggc gttccaactg accaagattt ccggcgccta ctggcgcggc gactcgaaga acgagcagtt gcaacgcatc tacggcaccg cctgggccga caagaagcaa ctggcggcct acatccagcg catcgaagag gccgagaagc gcgaccatcg ccgcatcggc aagcagctcg acctgttcca cctgcaggaa gaagcgccgg gcatggtgtt ctggcacccg aatggctgga gcgtctacca ggtgctcgag cagtacatgc gcaaggtcca gcgcgaccat ggctatgtcg aagtgcgtac cccgcagtgg tcgaccgcat cctctgggag cgttcgggcc actggtcgaa ctacgccnag aacatgttca ccnnttcctc ggaaagtcgc gactacncgg tcaagccgat gaactgcccg ngccacgtgc agatcttcac cagggcctga gtccnacncg acngcgctgc gcntcncnan tnngnncngc accgcacnnn cntncgggnn nctnnanggg ntcangcncg tacnngnnna nnnncnnnnn ntntcngnnc cnnnnacagg nnganaangg aanngncccn naatntc

Expected Protein Sequence

MAHHHHHHMP IITLPDGSQR SFDHPVSVAE VAQSIGAGLA KATLAGKVDG RLVDACDTID RDATLQIITP KDEEGLEIIR HSCAHLVGHA VKQLYPTAKM VIGPVIEEGF YYDIFFERPF TPEDMAAIQQ RMRELIDKDY DVIKKMTPRA EVIELFKSRG EDYKLRLIDD MPDEKAMGLY FHEEYVDMCR GPHVPNTRFL KAFQLTKISG AYWRGDSKNE QLQRIYGTAW ADKKQLAAYI QRIEEAEKRD HRRIGKQLDL FHLQEEAPGM VFWHPNGWSV YQVLEQYMRK VQRDHGYVEV RTPQVVDRIL WERSGHWSNY AENMFTTSSE SRDYAVKPMN CPCHVQIFNQ GLKSYRDLPL RLAEFGACHR NEPSGALHGI MRVRGFTQDD AHIFCTEEQV KKEAADFIKL TLQVYRDFGF TDIAMKLSTR PAKRVGSDEL WDRAEGALAD ALNESGLAWE YQPGEGAFYG PKIEFTLKDC LGRNWQCGTL QYDPNLPERL DASYIAEDNN RKRPVMLHRA ILGSFERFIG MLIEHYAGAF PAWLAPTQAV VMNITDKQAD FAAEVVRILG ESGFRAKSDL RNEKIGFKIR EHTLLKVPYL LVIGDREVES KAVAVRTREG EDLGSMPVTQ FAELLAQAVS RRGRQDSE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccca tcattactct tcccgacggc agtcaacgtt ccttcgatca cccggtctcc gtggccgagg tggcccaatc catcggcgcg ggcctggcca aggcgaccct cgccggcaag gtcgacggcc gcctggtcga cgcctgcgac accatcgatc gcgacgcgac cctgcagatc atcacgccca aggacgagga aggactggag atcatccgcc actcctgcgc ccacctggtc ggccatgcgg tcaagcagct ctatccgacc gcgaagatgg tcatcggccc ggtgatcgag gaaggcttct actacgacat tttcttcgag cgccccttca cccccgagga catggcggcg atccagcagc gcatgcgcga gctgatcgac aaggactacg acgtgatcaa gaagatgacc ccgcgcgccg aggtcatcga gctgttcaag tcccgtggcg aagactacaa gctgcgcctg atcgacgaca tgccggacga gaaggccatg ggcctgtact tccatgagga gtacgtggac atgtgccgcg gcccgcatgt gccgaacacc cgcttcctca aggcgttcca actgaccaag atttccggcg cctactggcg cggcgactcg aagaacgagc agttgcaacg catctacggc accgcctggg ccgacaagaa gcaactggcg gcctacatcc agcgcatcga agaggccgag aagcgcgacc atcgccgcat cggcaagcag ctcgacctgt tccacctgca ggaagaagcg ccgggcatgg tgttctggca cccgaatggc tggagcgtct accaggtgct cgagcagtac atgcgcaagg tccagcgcga ccatggctat gtcgaagtgc gtaccccgca ggtggtcgac cgcatcctct gggagcgttc gggccactgg tcgaactacg ccgagaacat gttcaccacc tcctcggaaa gtcgcgacta cgcggtcaag ccgatgaact gcccgtgcca cgtgcagatc ttcaaccagg gcctgaagtc ctaccgcgac ctgccgctgc gcctcgccga gttcggcgcc tgccaccgca acgagccgtc cggcgcgctg cacgggatca tgcgcgtacg cggctttacc caggacgacg cgcatatctt ctgcaccgaa gagcaggtga agaaggaagc ggccgatttc atcaagctga ccttgcaggt ctaccgcgac ttcggtttca ccgacatcgc catgaagctg tcgacccgtc cggccaagcg cgtcggttcc gacgagctgt gggatcgcgc cgaaggcgcg ctggccgatg cgctgaacga atccggcctg gcctgggaat accagccggg cgagggcgcg ttctacgggc cgaagatcga gttcaccctg aaggactgcc tcggccgtaa ctggcagtgc ggcaccctgc agtacgaccc gaacctgccg gagcgcctgg acgccagcta tatcgccgag gacaacaacc gtaagcgtcc ggtgatgctg caccgtgcga tcctcgggtc cttcgagcgc ttcatcggca tgctcatcga gcactacgcc ggagccttcc cggcctggct ggcgccgacc caggcagtgg tgatgaacat caccgacaag caggccgatt tcgccgccga ggtggtgcgg atcctcgggg aaagcggatt ccgtgccaag tccgacttga gaaacgagaa gatcggcttt aaaatccgcg agcatacttt gctcaaggtt ccctatctct tggttattgg agatcgggaa gttgaatcga aggccgtcgc ggtgcgtacg cgcgaagggg aagacctggg ctccatgccc gtcacccagt tcgctgagct gttggcacag gcggtttccc ggcgtggtcg ccaagactcg gagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for PsaeA.00156.a.B2.GE40429

HARVESTED ON:	7/15/2016
SEQUENCED ON:	7/18/2016
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccca tcattactct tcccgacggc agtcaacgtt ccttcgatca cccggtctcc gtggccgagg tggcccaatc catcggcgcg ggcctggcca aggcgaccct cgccggcaag gtcgacggcc gcctggtcga cgcctgcgac accatcgatc gcgacgcgac cctgcagatc atcacgccca aggacgagga aggactggag atcatccgcc actcctgcgc ccacctggtc ggccatgcgg tcaagcagct ctatccgacc gcgaagatgg tcatcggccc ggtgatcgag gaaggcttct actacgacat tttcttcgag cgccccttca cccccgagga catggcggcg atccagcagc gcatgcgcga gctgatcgac aaggactacg acgtgatcaa gaagatgacc ccgcgcgccg aggtcatcga gctgttcaag tcccgtggcg aagactacaa gctgcgcctg atcgacgaca tgccggacga gaaggccatg ggcctgtact tccatgagga gtacgtggac atgtgccgcg gcccgcatgt gccgaacacc cgcttcctca aggcgttcca actgaccaag atttccggcg cctactggcg cggcgactcg aagaacgagc agttgcaacg catctacggc accgcctggg ccgacaagaa gcaactggcg gcctacatcc agcgcatcga agaggccgag aagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat