PsaeA.00153.a
DNA primase (EC 2.7.7.-)
| CENTER ID: | PsaeA.00153.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00153.a.B1.GE38208 | full length | 1 | 664 | ||
| PsaeA.00153.a.B2.GE40423 | truncatin 1 | 1 | 424 | ||
| PsaeA.00153.a.B3.GE40424 | truncation 2 | 1 | 437 | ||
| PsaeA.00153.a.B4.GE40425 | truncation 3 | 1 | 486 | ||
| PsaeA.00153.a.B5.GE40426 | truncation 4 | 10 | 399 | ||
| PsaeA.00153.a.B6.GE40427 | truncation 5 | 106 | 424 | ||
| PsaeA.00153.a.B7.GE40428 | truncation 6 | 106 | 486 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00153.a.B1.PS02191 | full length | 1 | 664 | ||
| PsaeA.00153.a.B3.PS38102 | truncation 2 | 1 | 437 | ||
| PsaeA.00153.a.B6.PS38103 | truncation 5 | 106 | 424 | ||
| PsaeA.00153.a.B7.PS38108 | truncation 6 | 106 | 486 | ||
| PsaeA.00153.a.B2.PS38109 | truncatin 1 | 1 | 424 | ||
| PsaeA.00153.a.B2.PS38110 | truncatin 1 | 1 | 424 | ||
| PsaeA.00153.a.B4.PS38112 | truncation 3 | 1 | 486 |
Structures
5VAZ
DEPOSITED: 3/28/2017
DETERMINATION: XRay
CLONE: PsaeA.00153.a.B7.GE40428
PROTEIN: PsaeA.00153.a.B7.PS38108
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.612 |
| PGAT: | pseudomonas:PA0577 |
| RefSeq: | NP_249268.1 |
| UniProt: | Q9I5W0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGLIPQSFI DDLLNRTDIV EVVSSRIQLK KTGKNYSACC PFHKEKTPSF TVSPDKQFYY CFGCGAGGNA LGFVMDHDQL EFPQAVEELA KRAGMDVPRE ERGGRGHTPR QPTDSPLYPL LSAAAEFYKQ ALKSHPARKA AVNYLKGRGL TGEIARDFGL GFAPPGWDNL LKHLGGDNLQ LKAMLDAGLL VENSDTGKRY DRFRDRVMFP IRDSRGRIIA FGGRVLGDDK PKYLNSPETP VFHKGQELYG LYEARQKNRD LDEIMVVEGY MDVIALAQQG IRNAVATLGT ATSEEHIKRL FRLVPSILFC FDGDQAGRKA AWRALESVLP NLQDGKRVRF LFLPEGEDPD SLVRAEGEDA FRARITQQAQ PLAEYFFQQL MLEADPATLE GKAHLATLAA PLLEKIPGNN LRLLMRQRLS EITGLSGENI GQLAHHSPPP SSMDHGASGV LDGDDYFAAS AYYENEPSHA PFDAAPGYVE AQPRKSWNKD KKPWDGKKWD GKKKWDKGGR GDFKAPQRTP VSVESTTLNA LRTLLHHPQL ALKVDDAGTL AREQDTYAQL LVSLLEALQK NPRQSSMQLI ARWHGTPQGR LLQALGEKEW LIVQENLEKQ FFDTITKLSE SQRFGEREER LRSVMQKSYS ELTDEEKALL REHYSVAASS PSQS
NT Sequence
atggccggcc tgataccgca aagcttcatc gatgacctgc tgaaccgcac cgacatcgtc gaggtggtga gttcacgcat ccaactgaag aaaaccggca agaactacag cgcctgctgc ccgttccaca aggaaaagac tccctcgttc accgtcagcc cggacaagca gttctactac tgcttcggct gcggcgccgg cggcaacgcg ctcggcttcg tcatggacca cgaccagctg gagttccccc aggcggtcga ggaactggcc aagcgcgccg gcatggacgt gccgcgcgag gaacgcggcg gacgcgggca cacgccgcgc cagccgaccg actcgccgct ctacccgctg ctcagcgccg ccgccgagtt ctacaagcag gcgctgaaaa gccacccggc gcgcaaagcc gcggtgaact acctgaaggg ccgcggcctg accggcgaga tcgcccgcga cttcggcctc ggtttcgccc cgcccggctg ggacaacctg ctcaagcacc tgggtggcga caacctgcaa ctcaaggcca tgctcgacgc cggcctgctg gtggagaaca gcgacaccgg caagcgctac gaccgcttcc gcgatcgcgt gatgttcccg atccgcgaca gccgcgggcg gatcatcgcc ttcggcggcc gggtgctcgg cgacgacaag ccgaagtacc tgaactcccc ggagactccg gtcttccaca agggccagga actctacgga ctctacgagg cccggcagaa gaatcgcgac ctcgacgaga tcatggtggt cgagggctac atggatgtca tcgccctggc ccagcaaggc atccgcaacg cggtggcgac cctcggcacg gcgaccagcg aggaacacat caagcgcctg ttccgcctgg tgccgagcat cctgttctgt ttcgacggcg accaggccgg gcgcaaggcg gcctggcgcg ccctggagtc ggtgctgccg aacctgcagg acggcaagcg cgtgcgcttc ctgttcctcc ccgagggcga ggacccggac agcctggtac gcgccgaggg cgaggatgcc ttccgcgcac gcatcaccca gcaggcccag ccgctggcgg agtacttctt ccaacaactg atgctggagg ccgaccccgc caccctggaa ggcaaggccc acctggcgac cctcgccgcg ccgctgctgg agaagattcc cgggaacaac ctgcgcctgc tgatgcggca acgtctgtcg gaaatcaccg gcctgtccgg cgaaaacatc ggacaactgg cgcaccacag cccgccgccg tcgtccatgg accacggagc gagcggcgtc ttggacggcg acgactattt cgccgccagc gcctactacg agaacgagcc ctcccatgcg ccgttcgacg ctgcgccagg ctatgtcgag gcgcaaccgc gcaagagctg gaacaaggac aagaagccct gggacggcaa gaagtgggat ggcaagaaga agtgggacaa aggcggccgc ggcgacttca aggctccgca acgaacgccg gtcagcgtgg agtccaccac cctcaacgcc ctgcgcacct tgctgcatca tccgcagttg gccttgaagg tcgacgatgc gggcaccctc gccagggagc aggataccta cgcccagttg ctggtgtccc tgctggaagc cctgcagaag aacccgcggc agagctcgat gcaactcatc gcgcgctggc acggcacgcc ccagggccgc ctgctgcagg ccctgggcga gaaggagtgg ctgatcgtcc aggagaacct tgaaaagcag ttcttcgaca ccattactaa actctccgag agccagcgtt tcggcgagcg ggaggagcgt ttacgcagcg tcatgcagaa gagctacagt gaactgaccg atgaagagaa agccttgctc agagagcact acagtgttgc cgcttcatcg ccttcccaga gctga
Details for PsaeA.00153.a.B1.GE38208
| HARVESTED ON: | 6/11/2014 |
| SEQUENCED ON: | 6/12/2014 |
| EXPECTED MW: | 75kDa |
| OBSERVED MW: | 75kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 84 |
| PERCENT COVERAGE: | 58 |
Validated AA Sequence
MAHHHHHHMA GLIPQSFIDD LLNRTDIVEV VSSRIQLKKT GKNYSACCPF HKEKTPSFTV SPDKQFYYCF GCGAGGNALG FVMDHDQLEF PQAVEELAKR AGMDVPREER GGRGHTPRQP TDSPLYPLLS AAAEFYKQAL KSHPARKAAV NYLKGRGLTG EIARDFGLGF APPGWDNLLK HLGGDNLQLK AMLDAGLLVE NSDTGKRYDR FRDRVMFPIR DSRGRIIAFG GRVLGDDKPK YLNSPETPVF HKGQELYGLY EARQKNRDLD EIMVVEGYMD VIALAQQGIR NAVATLGTAT SEEHIKRLFR LVPSILSVST RQXGARRPXR LXRAAXAXAA RALXPPXGRD XXAXRXXGRX XXRXHXXXXX XWXXFSXXXS AXXXXXXXXX XLXXX
Validated NT Sequence
annncnntag anaattttnt ttaactttaa gaaggagata taccatggct caccaccacc accaccatat ggccggcctg ataccgcaaa gcttcatcga tgacctgctg aaccgcaccg acatcgtcga ggtggtgagt tcacgcatcc aactgaagaa aaccggcaag aactacagcg cctgctgccc gttccacaag gaaaagactc cctcgttcac cgtcagcccg gacaagcagt tctactactg cttcggctgc ggcgccggcg gcaacgcgct cggcttcgtc atggaccacg accagctgga gttcccccag gcggtcgagg aactggccaa gcgcgccggc atggacgtgc cgcgcgagga acgcggcgga cgcgggcaca cgccgcgcca gccgaccgac tcgccgctct acccgctgct cagcgccgcc gccgagttct acaagcaggc gctgaaaagc cacccggcgc gcaaagccgc ggtgaactac ctgaagggcc gcggcctgac cggcgagatc gcccgcgact tcggcctcgg tttcgccccg cccggctggg acaacctgct caagcacctg ggtggcgaca acctgcaact caaggccatg ctcgacgccg gcctgctggt ggagaacagc gacaccggca agcgctacga ccgcttccgc gatcgcgtga tgttcccgat ccgcgacagc cgcgggcgga tcatcgcctt cggcggccgg gtgctcggcg acgacaagcc gaagtacctg aactccccgg agactccggt cttccacaag ggccaggaac tctacggact ctacgaggcc cggcagaaga atcgcgacct cgacgagatc atggtggtcg agggctacat ggatgtcatc gccctggccc agcaaggcat ccgcaacgcg gtggcgaccc tcggcacggc gaccagcgag gaacacatca agcgcctgtt ccgcctggtg ccgagcatcc tgtctgtttc gacncgacag ncgggcgcaa ggcggcctgn gcgnctnnnt cgtgctgcga nngcngangc agcgcgtgcg ctnngtcctc ccnnagggcg agaccnnnca gctgnacgcn ccgangggcg agnngnnnng cgnnngcatn nnnannannn nnnnnnctgg cnnnacttct cnnnnnnctn nagtgctngn ngcnnnnnnn nncntgnnnn nannnnnctn nnncnnncnn nc
Expected Protein Sequence
MAHHHHHHMA GLIPQSFIDD LLNRTDIVEV VSSRIQLKKT GKNYSACCPF HKEKTPSFTV SPDKQFYYCF GCGAGGNALG FVMDHDQLEF PQAVEELAKR AGMDVPREER GGRGHTPRQP TDSPLYPLLS AAAEFYKQAL KSHPARKAAV NYLKGRGLTG EIARDFGLGF APPGWDNLLK HLGGDNLQLK AMLDAGLLVE NSDTGKRYDR FRDRVMFPIR DSRGRIIAFG GRVLGDDKPK YLNSPETPVF HKGQELYGLY EARQKNRDLD EIMVVEGYMD VIALAQQGIR NAVATLGTAT SEEHIKRLFR LVPSILFCFD GDQAGRKAAW RALESVLPNL QDGKRVRFLF LPEGEDPDSL VRAEGEDAFR ARITQQAQPL AEYFFQQLML EADPATLEGK AHLATLAAPL LEKIPGNNLR LLMRQRLSEI TGLSGENIGQ LAHHSPPPSS MDHGASGVLD GDDYFAASAY YENEPSHAPF DAAPGYVEAQ PRKSWNKDKK PWDGKKWDGK KKWDKGGRGD FKAPQRTPVS VESTTLNALR TLLHHPQLAL KVDDAGTLAR EQDTYAQLLV SLLEALQKNP RQSSMQLIAR WHGTPQGRLL QALGEKEWLI VQENLEKQFF DTITKLSESQ RFGEREERLR SVMQKSYSEL TDEEKALLRE HYSVAASSPS QS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg gcctgatacc gcaaagcttc atcgatgacc tgctgaaccg caccgacatc gtcgaggtgg tgagttcacg catccaactg aagaaaaccg gcaagaacta cagcgcctgc tgcccgttcc acaaggaaaa gactccctcg ttcaccgtca gcccggacaa gcagttctac tactgcttcg gctgcggcgc cggcggcaac gcgctcggct tcgtcatgga ccacgaccag ctggagttcc cccaggcggt cgaggaactg gccaagcgcg ccggcatgga cgtgccgcgc gaggaacgcg gcggacgcgg gcacacgccg cgccagccga ccgactcgcc gctctacccg ctgctcagcg ccgccgccga gttctacaag caggcgctga aaagccaccc ggcgcgcaaa gccgcggtga actacctgaa gggccgcggc ctgaccggcg agatcgcccg cgacttcggc ctcggtttcg ccccgcccgg ctgggacaac ctgctcaagc acctgggtgg cgacaacctg caactcaagg ccatgctcga cgccggcctg ctggtggaga acagcgacac cggcaagcgc tacgaccgct tccgcgatcg cgtgatgttc ccgatccgcg acagccgcgg gcggatcatc gccttcggcg gccgggtgct cggcgacgac aagccgaagt acctgaactc cccggagact ccggtcttcc acaagggcca ggaactctac ggactctacg aggcccggca gaagaatcgc gacctcgacg agatcatggt ggtcgagggc tacatggatg tcatcgccct ggcccagcaa ggcatccgca acgcggtggc gaccctcggc acggcgacca gcgaggaaca catcaagcgc ctgttccgcc tggtgccgag catcctgttc tgtttcgacg gcgaccaggc cgggcgcaag gcggcctggc gcgccctgga gtcggtgctg ccgaacctgc aggacggcaa gcgcgtgcgc ttcctgttcc tccccgaggg cgaggacccg gacagcctgg tacgcgccga gggcgaggat gccttccgcg cacgcatcac ccagcaggcc cagccgctgg cggagtactt cttccaacaa ctgatgctgg aggccgaccc cgccaccctg gaaggcaagg cccacctggc gaccctcgcc gcgccgctgc tggagaagat tcccgggaac aacctgcgcc tgctgatgcg gcaacgtctg tcggaaatca ccggcctgtc cggcgaaaac atcggacaac tggcgcacca cagcccgccg ccgtcgtcca tggaccacgg agcgagcggc gtcttggacg gcgacgacta tttcgccgcc agcgcctact acgagaacga gccctcccat gcgccgttcg acgctgcgcc aggctatgtc gaggcgcaac cgcgcaagag ctggaacaag gacaagaagc cctgggacgg caagaagtgg gatggcaaga agaagtggga caaaggcggc cgcggcgact tcaaggctcc gcaacgaacg ccggtcagcg tggagtccac caccctcaac gccctgcgca ccttgctgca tcatccgcag ttggccttga aggtcgacga tgcgggcacc ctcgccaggg agcaggatac ctacgcccag ttgctggtgt ccctgctgga agccctgcag aagaacccgc ggcagagctc gatgcaactc atcgcgcgct ggcacggcac gccccagggc cgcctgctgc aggccctggg cgagaaggag tggctgatcg tccaggagaa ccttgaaaag cagttcttcg acaccattac taaactctcc gagagccagc gtttcggcga gcgggaggag cgtttacgca gcgtcatgca gaagagctac agtgaactga ccgatgaaga gaaagccttg ctcagagagc actacagtgt tgccgcttca tcgccttccc agagctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for PsaeA.00153.a.B2.GE40423
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 48kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA GLIPQSFIDD LLNRTDIVEV VSSRIQLKKT GKNYSACCPF HKEKTPSFTV SPDKQFYYCF GCGAGGNALG FVMDHDQLEF PQAVEELAKR AGMDVPREER GGRGHTPRQP TDSPLYPLLS AAAEFYKQAL KSHPARKAAV NYLKGRGLTG EIARDFGLGF APPGWDNLLK HLGGDNLQLK AMLDAGLLVE NSDTGKRYDR FRDRVMFPIR DSRGRIIAFG GRVLGDDKPK YLNSPETPVF HKGQELYGLY EARQKNRDLD EIMVVEGYMD VIALAQQGIR NAVATLGTAT SEEHIKRLFR LVPSILFCFD GDQAGRKAAW RALESVLPNL QDGKRVRFLF LPEGEDPDSL VRAEGEDAFR ARITQQAQPL AEYFFQQLML EADPATLEGK AHLATLAAPL LEKIPGNNLR LLMRQRLSEI TG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg gcctgatacc gcaaagcttc atcgatgacc tgctgaaccg caccgacatc gtcgaggtgg tgagttcacg catccaactg aagaaaaccg gcaagaacta cagcgcctgc tgcccgttcc acaaggaaaa gactccctcg ttcaccgtca gcccggacaa gcagttctac tactgcttcg gctgcggcgc cggcggcaac gcgctcggct tcgtcatgga ccacgaccag ctggagttcc cccaggcggt cgaggaactg gccaagcgcg ccggcatgga cgtgccgcgc gaggaacgcg gcggacgcgg gcacacgccg cgccagccga ccgactcgcc gctctacccg ctgctcagcg ccgccgccga gttctacaag caggcgctga aaagccaccc ggcgcgcaaa gccgcggtga actacctgaa gggccgcggc ctgaccggcg agatcgcccg cgacttcggc ctcggtttcg ccccgcccgg ctgggacaac ctgctcaagc acctgggtgg cgacaacctg caactcaagg ccatgctcga cgccggcctg ctggtggaga acagcgacac cggcaagcgc tacgaccgct tccgcgatcg cgtgatgttc ccgatccgcg acagccgcgg gcggatcatc gccttcggcg gccgggtgct cggcgacgac aagccgaagt acctgaactc cccggagact ccggtcttcc acaagggcca ggaactctac ggactctacg aggcccggca gaagaatcgc gacctcgacg agatcatggt ggtcgagggc tacatggatg tcatcgccct ggcccagcaa ggcatccgca acgcggtggc gaccctcggc acggcgacca gcgaggaaca catcaagcgc ctgttccgcc tggtgccgag catcctgttc tgtttcgacg gcgaccaggc cgggcgcaag gcggcctggc gcgccctgga gtcggtgctg ccgaacctgc aggacggcaa gcgcgtgcgc ttcctgttcc tccccgaggg cgaggacccg gacagcctgg tacgcgccga gggcgaggat gccttccgcg cacgcatcac ccagcaggcc cagccgctgg cggagtactt cttccaacaa ctgatgctgg aggccgaccc cgccaccctg gaaggcaagg cccacctggc gaccctcgcc gcgccgctgc tggagaagat tcccgggaac aacctgcgcc tgctgatgcg gcaacgtctg tcggaaatca ccggctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for PsaeA.00153.a.B3.GE40424
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 50kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA GLIPQSFIDD LLNRTDIVEV VSSRIQLKKT GKNYSACCPF HKEKTPSFTV SPDKQFYYCF GCGAGGNALG FVMDHDQLEF PQAVEELAKR AGMDVPREER GGRGHTPRQP TDSPLYPLLS AAAEFYKQAL KSHPARKAAV NYLKGRGLTG EIARDFGLGF APPGWDNLLK HLGGDNLQLK AMLDAGLLVE NSDTGKRYDR FRDRVMFPIR DSRGRIIAFG GRVLGDDKPK YLNSPETPVF HKGQELYGLY EARQKNRDLD EIMVVEGYMD VIALAQQGIR NAVATLGTAT SEEHIKRLFR LVPSILFCFD GDQAGRKAAW RALESVLPNL QDGKRVRFLF LPEGEDPDSL VRAEGEDAFR ARITQQAQPL AEYFFQQLML EADPATLEGK AHLATLAAPL LEKIPGNNLR LLMRQRLSEI TGLSGENIGQ LAHHS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg gcctgatacc gcaaagcttc atcgatgacc tgctgaaccg caccgacatc gtcgaggtgg tgagttcacg catccaactg aagaaaaccg gcaagaacta cagcgcctgc tgcccgttcc acaaggaaaa gactccctcg ttcaccgtca gcccggacaa gcagttctac tactgcttcg gctgcggcgc cggcggcaac gcgctcggct tcgtcatgga ccacgaccag ctggagttcc cccaggcggt cgaggaactg gccaagcgcg ccggcatgga cgtgccgcgc gaggaacgcg gcggacgcgg gcacacgccg cgccagccga ccgactcgcc gctctacccg ctgctcagcg ccgccgccga gttctacaag caggcgctga aaagccaccc ggcgcgcaaa gccgcggtga actacctgaa gggccgcggc ctgaccggcg agatcgcccg cgacttcggc ctcggtttcg ccccgcccgg ctgggacaac ctgctcaagc acctgggtgg cgacaacctg caactcaagg ccatgctcga cgccggcctg ctggtggaga acagcgacac cggcaagcgc tacgaccgct tccgcgatcg cgtgatgttc ccgatccgcg acagccgcgg gcggatcatc gccttcggcg gccgggtgct cggcgacgac aagccgaagt acctgaactc cccggagact ccggtcttcc acaagggcca ggaactctac ggactctacg aggcccggca gaagaatcgc gacctcgacg agatcatggt ggtcgagggc tacatggatg tcatcgccct ggcccagcaa ggcatccgca acgcggtggc gaccctcggc acggcgacca gcgaggaaca catcaagcgc ctgttccgcc tggtgccgag catcctgttc tgtttcgacg gcgaccaggc cgggcgcaag gcggcctggc gcgccctgga gtcggtgctg ccgaacctgc aggacggcaa gcgcgtgcgc ttcctgttcc tccccgaggg cgaggacccg gacagcctgg tacgcgccga gggcgaggat gccttccgcg cacgcatcac ccagcaggcc cagccgctgg cggagtactt cttccaacaa ctgatgctgg aggccgaccc cgccaccctg gaaggcaagg cccacctggc gaccctcgcc gcgccgctgc tggagaagat tcccgggaac aacctgcgcc tgctgatgcg gcaacgtctg tcggaaatca ccggcctgtc cggcgaaaac atcggacaac tggcgcacca cagctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for PsaeA.00153.a.B4.GE40425
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 55kDa |
| OBSERVED MW: | 55kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA GLIPQSFIDD LLNRTDIVEV VSSRIQLKKT GKNYSACCPF HKEKTPSFTV SPDKQFYYCF GCGAGGNALG FVMDHDQLEF PQAVEELAKR AGMDVPREER GGRGHTPRQP TDSPLYPLLS AAAEFYKQAL KSHPARKAAV NYLKGRGLTG EIARDFGLGF APPGWDNLLK HLGGDNLQLK AMLDAGLLVE NSDTGKRYDR FRDRVMFPIR DSRGRIIAFG GRVLGDDKPK YLNSPETPVF HKGQELYGLY EARQKNRDLD EIMVVEGYMD VIALAQQGIR NAVATLGTAT SEEHIKRLFR LVPSILFCFD GDQAGRKAAW RALESVLPNL QDGKRVRFLF LPEGEDPDSL VRAEGEDAFR ARITQQAQPL AEYFFQQLML EADPATLEGK AHLATLAAPL LEKIPGNNLR LLMRQRLSEI TGLSGENIGQ LAHHSPPPSS MDHGASGVLD GDDYFAASAY YENEPSHAPF DAAPGYVEAQ PRKS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg gcctgatacc gcaaagcttc atcgatgacc tgctgaaccg caccgacatc gtcgaggtgg tgagttcacg catccaactg aagaaaaccg gcaagaacta cagcgcctgc tgcccgttcc acaaggaaaa gactccctcg ttcaccgtca gcccggacaa gcagttctac tactgcttcg gctgcggcgc cggcggcaac gcgctcggct tcgtcatgga ccacgaccag ctggagttcc cccaggcggt cgaggaactg gccaagcgcg ccggcatgga cgtgccgcgc gaggaacgcg gcggacgcgg gcacacgccg cgccagccga ccgactcgcc gctctacccg ctgctcagcg ccgccgccga gttctacaag caggcgctga aaagccaccc ggcgcgcaaa gccgcggtga actacctgaa gggccgcggc ctgaccggcg agatcgcccg cgacttcggc ctcggtttcg ccccgcccgg ctgggacaac ctgctcaagc acctgggtgg cgacaacctg caactcaagg ccatgctcga cgccggcctg ctggtggaga acagcgacac cggcaagcgc tacgaccgct tccgcgatcg cgtgatgttc ccgatccgcg acagccgcgg gcggatcatc gccttcggcg gccgggtgct cggcgacgac aagccgaagt acctgaactc cccggagact ccggtcttcc acaagggcca ggaactctac ggactctacg aggcccggca gaagaatcgc gacctcgacg agatcatggt ggtcgagggc tacatggatg tcatcgccct ggcccagcaa ggcatccgca acgcggtggc gaccctcggc acggcgacca gcgaggaaca catcaagcgc ctgttccgcc tggtgccgag catcctgttc tgtttcgacg gcgaccaggc cgggcgcaag gcggcctggc gcgccctgga gtcggtgctg ccgaacctgc aggacggcaa gcgcgtgcgc ttcctgttcc tccccgaggg cgaggacccg gacagcctgg tacgcgccga gggcgaggat gccttccgcg cacgcatcac ccagcaggcc cagccgctgg cggagtactt cttccaacaa ctgatgctgg aggccgaccc cgccaccctg gaaggcaagg cccacctggc gaccctcgcc gcgccgctgc tggagaagat tcccgggaac aacctgcgcc tgctgatgcg gcaacgtctg tcggaaatca ccggcctgtc cggcgaaaac atcggacaac tggcgcacca cagcccgccg ccgtcgtcca tggaccacgg agcgagcggc gtcttggacg gcgacgacta tttcgccgcc agcgcctact acgagaacga gccctcccat gcgccgttcg acgctgcgcc aggctatgtc gaggcgcaac cgcgcaagag ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for PsaeA.00153.a.B5.GE40426
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMI DDLLNRTDIV EVVSSRIQLK KTGKNYSACC PFHKEKTPSF TVSPDKQFYY CFGCGAGGNA LGFVMDHDQL EFPQAVEELA KRAGMDVPRE ERGGRGHTPR QPTDSPLYPL LSAAAEFYKQ ALKSHPARKA AVNYLKGRGL TGEIARDFGL GFAPPGWDNL LKHLGGDNLQ LKAMLDAGLL VENSDTGKRY DRFRDRVMFP IRDSRGRIIA FGGRVLGDDK PKYLNSPETP VFHKGQELYG LYEARQKNRD LDEIMVVEGY MDVIALAQQG IRNAVATLGT ATSEEHIKRL FRLVPSILFC FDGDQAGRKA AWRALESVLP NLQDGKRVRF LFLPEGEDPD SLVRAEGEDA FRARITQQAQ PLAEYFFQQL MLEADPATLE G
Validated NT Sequence
cttgccttcc agggtggcgg ggtcggcctc cagcatcagt tgttggaaga agtactccgc cagcggctgg gcctgctggg tgatgcgtgc gcggaaggca tcctcgccct cggcgcgtac caggctgtcc gggtcctcgc cctcggggag gaacaggaag cgcacgcgct tgccgtcctg caggttcggc agcaccgact ccagggcgcg ccaggccgcc ttgcgcccgg cctggtcgcc gtcgaaacag aacaggatgc tcggcaccag gcggaacagg cgcttgatgt gttcctcgct ggtcgccgtg ccgagggtcg ccaccgcgtt gcggatgcct tgctgggcca gggcgatgac atccatgtag ccctcgacca ccatgatctc gtcgaggtcg cgattcttct gccgggcctc gtagagtccg tagagttcct ggcccttgtg gaagaccgga gtctccgggg agttcaggta cttcggcttg tcgtcgccga gcacccggcc gccgaaggcg atgatccgcc cgcggctgtc gcggatcggg aacatcacgc gatcgcggaa gcggtcgtag cgcttgccgg tgtcgctgtt ctccaccagc aggccggcgt cgagcatggc cttgagttgc aggttgtcgc cacccaggtg cttgagcagg ttgtcccagc cgggcggggc gaaaccgagg ccgaagtcgc gggcgatctc gccggtcagg ccgcggccct tcaggtagtt caccgcggct ttgcgcgccg ggtggctttt cagcgcctgc ttgtagaact cggcggcggc gctgagcagc gggtagagcg gcgagtcggt cggctggcgc ggcgtgtgcc cgcgtccgcc gcgttcctcg cgcggcacgt ccatgccggc gcgcttggcc agttcctcga ccgcctgggg gaactccagc tggtcgtggt ccatgacgaa gccgagcgcg ttgccgccgg cgccgcagcc gaagcagtag tagaactgct tgtccgggct gacggtgaac gagggagtct tttccttgtg gaacgggcag caggcgctgt agttcttgcc ggttttcttc agttggatgc gtgaactcac cacctcgacg atgtcggtgc ggttcagcag gtcatcgatc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHID DLLNRTDIVE VVSSRIQLKK TGKNYSACCP FHKEKTPSFT VSPDKQFYYC FGCGAGGNAL GFVMDHDQLE FPQAVEELAK RAGMDVPREE RGGRGHTPRQ PTDSPLYPLL SAAAEFYKQA LKSHPARKAA VNYLKGRGLT GEIARDFGLG FAPPGWDNLL KHLGGDNLQL KAMLDAGLLV ENSDTGKRYD RFRDRVMFPI RDSRGRIIAF GGRVLGDDKP KYLNSPETPV FHKGQELYGL YEARQKNRDL DEIMVVEGYM DVIALAQQGI RNAVATLGTA TSEEHIKRLF RLVPSILFCF DGDQAGRKAA WRALESVLPN LQDGKRVRFL FLPEGEDPDS LVRAEGEDAF RARITQQAQP LAEYFFQQLM LEADPATLEG KAHLATLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatcgatg acctgctgaa ccgcaccgac atcgtcgagg tggtgagttc acgcatccaa ctgaagaaaa ccggcaagaa ctacagcgcc tgctgcccgt tccacaagga aaagactccc tcgttcaccg tcagcccgga caagcagttc tactactgct tcggctgcgg cgccggcggc aacgcgctcg gcttcgtcat ggaccacgac cagctggagt tcccccaggc ggtcgaggaa ctggccaagc gcgccggcat ggacgtgccg cgcgaggaac gcggcggacg cgggcacacg ccgcgccagc cgaccgactc gccgctctac ccgctgctca gcgccgccgc cgagttctac aagcaggcgc tgaaaagcca cccggcgcgc aaagccgcgg tgaactacct gaagggccgc ggcctgaccg gcgagatcgc ccgcgacttc ggcctcggtt tcgccccgcc cggctgggac aacctgctca agcacctggg tggcgacaac ctgcaactca aggccatgct cgacgccggc ctgctggtgg agaacagcga caccggcaag cgctacgacc gcttccgcga tcgcgtgatg ttcccgatcc gcgacagccg cgggcggatc atcgccttcg gcggccgggt gctcggcgac gacaagccga agtacctgaa ctccccggag actccggtct tccacaaggg ccaggaactc tacggactct acgaggcccg gcagaagaat cgcgacctcg acgagatcat ggtggtcgag ggctacatgg atgtcatcgc cctggcccag caaggcatcc gcaacgcggt ggcgaccctc ggcacggcga ccagcgagga acacatcaag cgcctgttcc gcctggtgcc gagcatcctg ttctgtttcg acggcgacca ggccgggcgc aaggcggcct ggcgcgccct ggagtcggtg ctgccgaacc tgcaggacgg caagcgcgtg cgcttcctgt tcctccccga gggcgaggac ccggacagcc tggtacgcgc cgagggcgag gatgccttcc gcgcacgcat cacccagcag gcccagccgc tggcggagta cttcttccaa caactgatgc tggaggccga ccccgccacc ctggaaggca aggcccacct ggcgaccctc gcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PsaeA.00153.a.B6.GE40427
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG HTPRQPTDSP LYPLLSAAAE FYKQALKSHP ARKAAVNYLK GRGLTGEIAR DFGLGFAPPG WDNLLKHLGG DNLQLKAMLD AGLLVENSDT GKRYDRFRDR VMFPIRDSRG RIIAFGGRVL GDDKPKYLNS PETPVFHKGQ ELYGLYEARQ KNRDLDEIMV VEGYMDVIAL AQQGIRNAVA TLGTATSEEH IKRLFRLVPS ILFCFDGDQA GRKAAWRALE SVLPNLQDGK RVRFLFLPEG EDPDSLVRAE GEDAFRARIT QQAQPLAEYF FQQLMLEADP ATLEGKAHLA TLAAPLLEKI PGNNLRLLMR QRLSEITG
Validated NT Sequence
cacttagccg gtgatttccg acagacgttg ccgcatcagc aggcgcaggt tgttcccggg aatcttctcc agcagcggcg cggcgagggt cgccaggtgg gccttgcctt ccagggtggc ggggtcggcc tccagcatca gttgttggaa gaagtactcc gccagcggct gggcctgctg ggtgatgcgt gcgcggaagg catcctcgcc ctcggcgcgt accaggctgt ccgggtcctc gccctcgggg aggaacagga agcgcacgcg cttgccgtcc tgcaggttcg gcagcaccga ctccagggcg cgccaggccg ccttgcgccc ggcctggtcg ccgtcgaaac agaacaggat gctcggcacc aggcggaaca ggcgcttgat gtgttcctcg ctggtcgccg tgccgagggt cgccaccgcg ttgcggatgc cttgctgggc cagggcgatg acatccatgt agccctcgac caccatgatc tcgtcgaggt cgcgattctt ctgccgggcc tcgtagagtc cgtagagttc ctggcccttg tggaagaccg gagtctccgg ggagttcagg tacttcggct tgtcgtcgcc gagcacccgg ccgccgaagg cgatgatccg cccgcggctg tcgcggatcg ggaacatcac gcgatcgcgg aagcggtcgt agcgcttgcc ggtgtcgctg ttctccacca gcaggccggc gtcgagcatg gccttgagtt gcaggttgtc gccacccagg tgcttgagca ggttgtccca gccgggcggg gcgaaaccga ggccgaagtc gcgggcgatc tcgccggtca ggccgcggcc cttcaggtag ttcaccgcgg ctttgcgcgc cgggtggctt ttcagcgcct gcttgtagaa ctcggcggcg gcgctgagca gcgggtagag cggcgagtcg gtcggctggc gcggcgtgtg ccccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHGH TPRQPTDSPL YPLLSAAAEF YKQALKSHPA RKAAVNYLKG RGLTGEIARD FGLGFAPPGW DNLLKHLGGD NLQLKAMLDA GLLVENSDTG KRYDRFRDRV MFPIRDSRGR IIAFGGRVLG DDKPKYLNSP ETPVFHKGQE LYGLYEARQK NRDLDEIMVV EGYMDVIALA QQGIRNAVAT LGTATSEEHI KRLFRLVPSI LFCFDGDQAG RKAAWRALES VLPNLQDGKR VRFLFLPEGE DPDSLVRAEG EDAFRARITQ QAQPLAEYFF QQLMLEADPA TLEGKAHLAT LAAPLLEKIP GNNLRLLMRQ RLSEITG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgggcaca cgccgcgcca gccgaccgac tcgccgctct acccgctgct cagcgccgcc gccgagttct acaagcaggc gctgaaaagc cacccggcgc gcaaagccgc ggtgaactac ctgaagggcc gcggcctgac cggcgagatc gcccgcgact tcggcctcgg tttcgccccg cccggctggg acaacctgct caagcacctg ggtggcgaca acctgcaact caaggccatg ctcgacgccg gcctgctggt ggagaacagc gacaccggca agcgctacga ccgcttccgc gatcgcgtga tgttcccgat ccgcgacagc cgcgggcgga tcatcgcctt cggcggccgg gtgctcggcg acgacaagcc gaagtacctg aactccccgg agactccggt cttccacaag ggccaggaac tctacggact ctacgaggcc cggcagaaga atcgcgacct cgacgagatc atggtggtcg agggctacat ggatgtcatc gccctggccc agcaaggcat ccgcaacgcg gtggcgaccc tcggcacggc gaccagcgag gaacacatca agcgcctgtt ccgcctggtg ccgagcatcc tgttctgttt cgacggcgac caggccgggc gcaaggcggc ctggcgcgcc ctggagtcgg tgctgccgaa cctgcaggac ggcaagcgcg tgcgcttcct gttcctcccc gagggcgagg acccggacag cctggtacgc gccgagggcg aggatgcctt ccgcgcacgc atcacccagc aggcccagcc gctggcggag tacttcttcc aacaactgat gctggaggcc gaccccgcca ccctggaagg caaggcccac ctggcgaccc tcgccgcgcc gctgctggag aagattcccg ggaacaacct gcgcctgctg atgcggcaac gtctgtcgga aatcaccggc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for PsaeA.00153.a.B7.GE40428
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/22/2016 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHGH TPRQPTDSPL YPLLSAAAEF YKQALKSHPA RKAAVNYLKG RGLTGEIARD FGLGFAPPGW DNLLKHLGGD NLQLKAMLDA GLLVENSDTG KRYDRFRDRV MFPIRDSRGR IIAFGGRVLG DDKPKYLNSP ETPVFHKGQE LYGLYEARQK NRDLDEIMVV EGYMDVIALA QQGIRNAVAT LGTATSEEHI KRLFRLVPSI LFCFDGDQAG RKAAWRALES VLPNLQDGKR VRFLFLPEGE DPDSLVRAEG EDAFRARITQ QAQPLAEYFF QQLMLEADPA TLEGKAHLAT LAAPLLEKIP GNNLRLLMRQ RLSEITGLSG ENIGQLAHHS PPPSSMDHGA SGVLDGDDYF AASAYYENEP SHAPFDAAPG YVEAQPRKS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgggcaca cgccgcgcca gccgaccgac tcgccgctct acccgctgct cagcgccgcc gccgagttct acaagcaggc gctgaaaagc cacccggcgc gcaaagccgc ggtgaactac ctgaagggcc gcggcctgac cggcgagatc gcccgcgact tcggcctcgg tttcgccccg cccggctggg acaacctgct caagcacctg ggtggcgaca acctgcaact caaggccatg ctcgacgccg gcctgctggt ggagaacagc gacaccggca agcgctacga ccgcttccgc gatcgcgtga tgttcccgat ccgcgacagc cgcgggcgga tcatcgcctt cggcggccgg gtgctcggcg acgacaagcc gaagtacctg aactccccgg agactccggt cttccacaag ggccaggaac tctacggact ctacgaggcc cggcagaaga atcgcgacct cgacgagatc atggtggtcg agggctacat ggatgtcatc gccctggccc agcaaggcat ccgcaacgcg gtggcgaccc tcggcacggc gaccagcgag gaacacatca agcgcctgtt ccgcctggtg ccgagcatcc tgttctgttt cgacggcgac caggccgggc gcaaggcggc ctggcgcgcc ctggagtcgg tgctgccgaa cctgcaggac ggcaagcgcg tgcgcttcct gttcctcccc gagggcgagg acccggacag cctggtacgc gccgagggcg aggatgcctt ccgcgcacgc atcacccagc aggcccagcc gctggcggag tacttcttcc aacaactgat gctggaggcc gaccccgcca ccctggaagg caaggcccac ctggcgaccc tcgccgcgcc gctgctggag aagattcccg ggaacaacct gcgcctgctg atgcggcaac gtctgtcgga aatcaccggc ctgtccggcg aaaacatcgg acaactggcg caccacagcc cgccgccgtc gtccatggac cacggagcga gcggcgtctt ggacggcgac gactatttcg ccgccagcgc ctactacgag aacgagccct cccatgcgcc gttcgacgct gcgccaggct atgtcgaggc gcaaccgcgc aagagctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for PsaeA.00153.a.B1.PS02191
| PURIFICATION DATe: | 10/21/2014 |
| CONCENTRATION: | 2mg/ml |
| OBSERVED MW: | 72kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 84 |
| PERCENT COVERAGE: | 58 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA GLIPQSFIDD LLNRTDIVEV VSSRIQLKKT GKNYSACCPF HKEKTPSFTV SPDKQFYYCF GCGAGGNALG FVMDHDQLEF PQAVEELAKR AGMDVPREER GGRGHTPRQP TDSPLYPLLS AAAEFYKQAL KSHPARKAAV NYLKGRGLTG EIARDFGLGF APPGWDNLLK HLGGDNLQLK AMLDAGLLVE NSDTGKRYDR FRDRVMFPIR DSRGRIIAFG GRVLGDDKPK YLNSPETPVF HKGQELYGLY EARQKNRDLD EIMVVEGYMD VIALAQQGIR NAVATLGTAT SEEHIKRLFR LVPSILSVST RQXGARRPXR LXRAAXAXAA RALXPPXGRD XXAXRXXGRX XXRXHXXXXX XWXXFSXXXS AXXXXXXXXX XLXXX
Validated NT Sequence
annncnntag anaattttnt ttaactttaa gaaggagata taccatggct caccaccacc accaccatat ggccggcctg ataccgcaaa gcttcatcga tgacctgctg aaccgcaccg acatcgtcga ggtggtgagt tcacgcatcc aactgaagaa aaccggcaag aactacagcg cctgctgccc gttccacaag gaaaagactc cctcgttcac cgtcagcccg gacaagcagt tctactactg cttcggctgc ggcgccggcg gcaacgcgct cggcttcgtc atggaccacg accagctgga gttcccccag gcggtcgagg aactggccaa gcgcgccggc atggacgtgc cgcgcgagga acgcggcgga cgcgggcaca cgccgcgcca gccgaccgac tcgccgctct acccgctgct cagcgccgcc gccgagttct acaagcaggc gctgaaaagc cacccggcgc gcaaagccgc ggtgaactac ctgaagggcc gcggcctgac cggcgagatc gcccgcgact tcggcctcgg tttcgccccg cccggctggg acaacctgct caagcacctg ggtggcgaca acctgcaact caaggccatg ctcgacgccg gcctgctggt ggagaacagc gacaccggca agcgctacga ccgcttccgc gatcgcgtga tgttcccgat ccgcgacagc cgcgggcgga tcatcgcctt cggcggccgg gtgctcggcg acgacaagcc gaagtacctg aactccccgg agactccggt cttccacaag ggccaggaac tctacggact ctacgaggcc cggcagaaga atcgcgacct cgacgagatc atggtggtcg agggctacat ggatgtcatc gccctggccc agcaaggcat ccgcaacgcg gtggcgaccc tcggcacggc gaccagcgag gaacacatca agcgcctgtt ccgcctggtg ccgagcatcc tgtctgtttc gacncgacag ncgggcgcaa ggcggcctgn gcgnctnnnt cgtgctgcga nngcngangc agcgcgtgcg ctnngtcctc ccnnagggcg agaccnnnca gctgnacgcn ccgangggcg agnngnnnng cgnnngcatn nnnannannn nnnnnnctgg cnnnacttct cnnnnnnctn nagtgctngn ngcnnnnnnn nncntgnnnn nannnnnctn nnncnnncnn nc
Expressed Protein Sequence
MAHHHHHHMA GLIPQSFIDD LLNRTDIVEV VSSRIQLKKT GKNYSACCPF HKEKTPSFTV SPDKQFYYCF GCGAGGNALG FVMDHDQLEF PQAVEELAKR AGMDVPREER GGRGHTPRQP TDSPLYPLLS AAAEFYKQAL KSHPARKAAV NYLKGRGLTG EIARDFGLGF APPGWDNLLK HLGGDNLQLK AMLDAGLLVE NSDTGKRYDR FRDRVMFPIR DSRGRIIAFG GRVLGDDKPK YLNSPETPVF HKGQELYGLY EARQKNRDLD EIMVVEGYMD VIALAQQGIR NAVATLGTAT SEEHIKRLFR LVPSILFCFD GDQAGRKAAW RALESVLPNL QDGKRVRFLF LPEGEDPDSL VRAEGEDAFR ARITQQAQPL AEYFFQQLML EADPATLEGK AHLATLAAPL LEKIPGNNLR LLMRQRLSEI TGLSGENIGQ LAHHSPPPSS MDHGASGVLD GDDYFAASAY YENEPSHAPF DAAPGYVEAQ PRKSWNKDKK PWDGKKWDGK KKWDKGGRGD FKAPQRTPVS VESTTLNALR TLLHHPQLAL KVDDAGTLAR EQDTYAQLLV SLLEALQKNP RQSSMQLIAR WHGTPQGRLL QALGEKEWLI VQENLEKQFF DTITKLSESQ RFGEREERLR SVMQKSYSEL TDEEKALLRE HYSVAASSPS QS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCG GCCTGATACC GCAAAGCTTC ATCGATGACC TGCTGAACCG CACCGACATC GTCGAGGTGG TGAGTTCACG CATCCAACTG AAGAAAACCG GCAAGAACTA CAGCGCCTGC TGCCCGTTCC ACAAGGAAAA GACTCCCTCG TTCACCGTCA GCCCGGACAA GCAGTTCTAC TACTGCTTCG GCTGCGGCGC CGGCGGCAAC GCGCTCGGCT TCGTCATGGA CCACGACCAG CTGGAGTTCC CCCAGGCGGT CGAGGAACTG GCCAAGCGCG CCGGCATGGA CGTGCCGCGC GAGGAACGCG GCGGACGCGG GCACACGCCG CGCCAGCCGA CCGACTCGCC GCTCTACCCG CTGCTCAGCG CCGCCGCCGA GTTCTACAAG CAGGCGCTGA AAAGCCACCC GGCGCGCAAA GCCGCGGTGA ACTACCTGAA GGGCCGCGGC CTGACCGGCG AGATCGCCCG CGACTTCGGC CTCGGTTTCG CCCCGCCCGG CTGGGACAAC CTGCTCAAGC ACCTGGGTGG CGACAACCTG CAACTCAAGG CCATGCTCGA CGCCGGCCTG CTGGTGGAGA ACAGCGACAC CGGCAAGCGC TACGACCGCT TCCGCGATCG CGTGATGTTC CCGATCCGCG ACAGCCGCGG GCGGATCATC GCCTTCGGCG GCCGGGTGCT CGGCGACGAC AAGCCGAAGT ACCTGAACTC CCCGGAGACT CCGGTCTTCC ACAAGGGCCA GGAACTCTAC GGACTCTACG AGGCCCGGCA GAAGAATCGC GACCTCGACG AGATCATGGT GGTCGAGGGC TACATGGATG TCATCGCCCT GGCCCAGCAA GGCATCCGCA ACGCGGTGGC GACCCTCGGC ACGGCGACCA GCGAGGAACA CATCAAGCGC CTGTTCCGCC TGGTGCCGAG CATCCTGTTC TGTTTCGACG GCGACCAGGC CGGGCGCAAG GCGGCCTGGC GCGCCCTGGA GTCGGTGCTG CCGAACCTGC AGGACGGCAA GCGCGTGCGC TTCCTGTTCC TCCCCGAGGG CGAGGACCCG GACAGCCTGG TACGCGCCGA GGGCGAGGAT GCCTTCCGCG CACGCATCAC CCAGCAGGCC CAGCCGCTGG CGGAGTACTT CTTCCAACAA CTGATGCTGG AGGCCGACCC CGCCACCCTG GAAGGCAAGG CCCACCTGGC GACCCTCGCC GCGCCGCTGC TGGAGAAGAT TCCCGGGAAC AACCTGCGCC TGCTGATGCG GCAACGTCTG TCGGAAATCA CCGGCCTGTC CGGCGAAAAC ATCGGACAAC TGGCGCACCA CAGCCCGCCG CCGTCGTCCA TGGACCACGG AGCGAGCGGC GTCTTGGACG GCGACGACTA TTTCGCCGCC AGCGCCTACT ACGAGAACGA GCCCTCCCAT GCGCCGTTCG ACGCTGCGCC AGGCTATGTC GAGGCGCAAC CGCGCAAGAG CTGGAACAAG GACAAGAAGC CCTGGGACGG CAAGAAGTGG GATGGCAAGA AGAAGTGGGA CAAAGGCGGC CGCGGCGACT TCAAGGCTCC GCAACGAACG CCGGTCAGCG TGGAGTCCAC CACCCTCAAC GCCCTGCGCA CCTTGCTGCA TCATCCGCAG TTGGCCTTGA AGGTCGACGA TGCGGGCACC CTCGCCAGGG AGCAGGATAC CTACGCCCAG TTGCTGGTGT CCCTGCTGGA AGCCCTGCAG AAGAACCCGC GGCAGAGCTC GATGCAACTC ATCGCGCGCT GGCACGGCAC GCCCCAGGGC CGCCTGCTGC AGGCCCTGGG CGAGAAGGAG TGGCTGATCG TCCAGGAGAA CCTTGAAAAG CAGTTCTTCG ACACCATTAC TAAACTCTCC GAGAGCCAGC GTTTCGGCGA GCGGGAGGAG CGTTTACGCA GCGTCATGCA GAAGAGCTAC AGTGAACTGA CCGATGAAGA GAAAGCCTTG CTCAGAGAGC ACTACAGTGT TGCCGCTTCA TCGCCTTCCC AGAGCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT
Details for PsaeA.00153.a.B3.PS38102
| PURIFICATION DATe: | 11/1/2016 |
| CONCENTRATION: | 16.34mg/ml |
| OBSERVED MW: | 49kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMA GLIPQSFIDD LLNRTDIVEV VSSRIQLKKT GKNYSACCPF HKEKTPSFTV SPDKQFYYCF GCGAGGNALG FVMDHDQLEF PQAVEELAKR AGMDVPREER GGRGHTPRQP TDSPLYPLLS AAAEFYKQAL KSHPARKAAV NYLKGRGLTG EIARDFGLGF APPGWDNLLK HLGGDNLQLK AMLDAGLLVE NSDTGKRYDR FRDRVMFPIR DSRGRIIAFG GRVLGDDKPK YLNSPETPVF HKGQELYGLY EARQKNRDLD EIMVVEGYMD VIALAQQGIR NAVATLGTAT SEEHIKRLFR LVPSILFCFD GDQAGRKAAW RALESVLPNL QDGKRVRFLF LPEGEDPDSL VRAEGEDAFR ARITQQAQPL AEYFFQQLML EADPATLEGK AHLATLAAPL LEKIPGNNLR LLMRQRLSEI TGLSGENIGQ LAHHS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCG GCCTGATACC GCAAAGCTTC ATCGATGACC TGCTGAACCG CACCGACATC GTCGAGGTGG TGAGTTCACG CATCCAACTG AAGAAAACCG GCAAGAACTA CAGCGCCTGC TGCCCGTTCC ACAAGGAAAA GACTCCCTCG TTCACCGTCA GCCCGGACAA GCAGTTCTAC TACTGCTTCG GCTGCGGCGC CGGCGGCAAC GCGCTCGGCT TCGTCATGGA CCACGACCAG CTGGAGTTCC CCCAGGCGGT CGAGGAACTG GCCAAGCGCG CCGGCATGGA CGTGCCGCGC GAGGAACGCG GCGGACGCGG GCACACGCCG CGCCAGCCGA CCGACTCGCC GCTCTACCCG CTGCTCAGCG CCGCCGCCGA GTTCTACAAG CAGGCGCTGA AAAGCCACCC GGCGCGCAAA GCCGCGGTGA ACTACCTGAA GGGCCGCGGC CTGACCGGCG AGATCGCCCG CGACTTCGGC CTCGGTTTCG CCCCGCCCGG CTGGGACAAC CTGCTCAAGC ACCTGGGTGG CGACAACCTG CAACTCAAGG CCATGCTCGA CGCCGGCCTG CTGGTGGAGA ACAGCGACAC CGGCAAGCGC TACGACCGCT TCCGCGATCG CGTGATGTTC CCGATCCGCG ACAGCCGCGG GCGGATCATC GCCTTCGGCG GCCGGGTGCT CGGCGACGAC AAGCCGAAGT ACCTGAACTC CCCGGAGACT CCGGTCTTCC ACAAGGGCCA GGAACTCTAC GGACTCTACG AGGCCCGGCA GAAGAATCGC GACCTCGACG AGATCATGGT GGTCGAGGGC TACATGGATG TCATCGCCCT GGCCCAGCAA GGCATCCGCA ACGCGGTGGC GACCCTCGGC ACGGCGACCA GCGAGGAACA CATCAAGCGC CTGTTCCGCC TGGTGCCGAG CATCCTGTTC TGTTTCGACG GCGACCAGGC CGGGCGCAAG GCGGCCTGGC GCGCCCTGGA GTCGGTGCTG CCGAACCTGC AGGACGGCAA GCGCGTGCGC TTCCTGTTCC TCCCCGAGGG CGAGGACCCG GACAGCCTGG TACGCGCCGA GGGCGAGGAT GCCTTCCGCG CACGCATCAC CCAGCAGGCC CAGCCGCTGG CGGAGTACTT CTTCCAACAA CTGATGCTGG AGGCCGACCC CGCCACCCTG GAAGGCAAGG CCCACCTGGC GACCCTCGCC GCGCCGCTGC TGGAGAAGAT TCCCGGGAAC AACCTGCGCC TGCTGATGCG GCAACGTCTG TCGGAAATCA CCGGCCTGTC CGGCGAAAAC ATCGGACAAC TGGCGCACCA CAGCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT
Details for PsaeA.00153.a.B6.PS38103
| PURIFICATION DATe: | 11/1/2016 |
| CONCENTRATION: | 42.86mg/ml |
| OBSERVED MW: | 37kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG HTPRQPTDSP LYPLLSAAAE FYKQALKSHP ARKAAVNYLK GRGLTGEIAR DFGLGFAPPG WDNLLKHLGG DNLQLKAMLD AGLLVENSDT GKRYDRFRDR VMFPIRDSRG RIIAFGGRVL GDDKPKYLNS PETPVFHKGQ ELYGLYEARQ KNRDLDEIMV VEGYMDVIAL AQQGIRNAVA TLGTATSEEH IKRLFRLVPS ILFCFDGDQA GRKAAWRALE SVLPNLQDGK RVRFLFLPEG EDPDSLVRAE GEDAFRARIT QQAQPLAEYF FQQLMLEADP ATLEGKAHLA TLAAPLLEKI PGNNLRLLMR QRLSEITG
Validated NT Sequence
cacttagccg gtgatttccg acagacgttg ccgcatcagc aggcgcaggt tgttcccggg aatcttctcc agcagcggcg cggcgagggt cgccaggtgg gccttgcctt ccagggtggc ggggtcggcc tccagcatca gttgttggaa gaagtactcc gccagcggct gggcctgctg ggtgatgcgt gcgcggaagg catcctcgcc ctcggcgcgt accaggctgt ccgggtcctc gccctcgggg aggaacagga agcgcacgcg cttgccgtcc tgcaggttcg gcagcaccga ctccagggcg cgccaggccg ccttgcgccc ggcctggtcg ccgtcgaaac agaacaggat gctcggcacc aggcggaaca ggcgcttgat gtgttcctcg ctggtcgccg tgccgagggt cgccaccgcg ttgcggatgc cttgctgggc cagggcgatg acatccatgt agccctcgac caccatgatc tcgtcgaggt cgcgattctt ctgccgggcc tcgtagagtc cgtagagttc ctggcccttg tggaagaccg gagtctccgg ggagttcagg tacttcggct tgtcgtcgcc gagcacccgg ccgccgaagg cgatgatccg cccgcggctg tcgcggatcg ggaacatcac gcgatcgcgg aagcggtcgt agcgcttgcc ggtgtcgctg ttctccacca gcaggccggc gtcgagcatg gccttgagtt gcaggttgtc gccacccagg tgcttgagca ggttgtccca gccgggcggg gcgaaaccga ggccgaagtc gcgggcgatc tcgccggtca ggccgcggcc cttcaggtag ttcaccgcgg ctttgcgcgc cgggtggctt ttcagcgcct gcttgtagaa ctcggcggcg gcgctgagca gcgggtagag cggcgagtcg gtcggctggc gcggcgtgtg ccccatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHGH TPRQPTDSPL YPLLSAAAEF YKQALKSHPA RKAAVNYLKG RGLTGEIARD FGLGFAPPGW DNLLKHLGGD NLQLKAMLDA GLLVENSDTG KRYDRFRDRV MFPIRDSRGR IIAFGGRVLG DDKPKYLNSP ETPVFHKGQE LYGLYEARQK NRDLDEIMVV EGYMDVIALA QQGIRNAVAT LGTATSEEHI KRLFRLVPSI LFCFDGDQAG RKAAWRALES VLPNLQDGKR VRFLFLPEGE DPDSLVRAEG EDAFRARITQ QAQPLAEYFF QQLMLEADPA TLEGKAHLAT LAAPLLEKIP GNNLRLLMRQ RLSEITG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGGCACA CGCCGCGCCA GCCGACCGAC TCGCCGCTCT ACCCGCTGCT CAGCGCCGCC GCCGAGTTCT ACAAGCAGGC GCTGAAAAGC CACCCGGCGC GCAAAGCCGC GGTGAACTAC CTGAAGGGCC GCGGCCTGAC CGGCGAGATC GCCCGCGACT TCGGCCTCGG TTTCGCCCCG CCCGGCTGGG ACAACCTGCT CAAGCACCTG GGTGGCGACA ACCTGCAACT CAAGGCCATG CTCGACGCCG GCCTGCTGGT GGAGAACAGC GACACCGGCA AGCGCTACGA CCGCTTCCGC GATCGCGTGA TGTTCCCGAT CCGCGACAGC CGCGGGCGGA TCATCGCCTT CGGCGGCCGG GTGCTCGGCG ACGACAAGCC GAAGTACCTG AACTCCCCGG AGACTCCGGT CTTCCACAAG GGCCAGGAAC TCTACGGACT CTACGAGGCC CGGCAGAAGA ATCGCGACCT CGACGAGATC ATGGTGGTCG AGGGCTACAT GGATGTCATC GCCCTGGCCC AGCAAGGCAT CCGCAACGCG GTGGCGACCC TCGGCACGGC GACCAGCGAG GAACACATCA AGCGCCTGTT CCGCCTGGTG CCGAGCATCC TGTTCTGTTT CGACGGCGAC CAGGCCGGGC GCAAGGCGGC CTGGCGCGCC CTGGAGTCGG TGCTGCCGAA CCTGCAGGAC GGCAAGCGCG TGCGCTTCCT GTTCCTCCCC GAGGGCGAGG ACCCGGACAG CCTGGTACGC GCCGAGGGCG AGGATGCCTT CCGCGCACGC ATCACCCAGC AGGCCCAGCC GCTGGCGGAG TACTTCTTCC AACAACTGAT GCTGGAGGCC GACCCCGCCA CCCTGGAAGG CAAGGCCCAC CTGGCGACCC TCGCCGCGCC GCTGCTGGAG AAGATTCCCG GGAACAACCT GCGCCTGCTG ATGCGGCAAC GTCTGTCGGA AATCACCGGC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for PsaeA.00153.a.B7.PS38108
| PURIFICATION DATe: | 11/8/2016 |
| CONCENTRATION: | 57.73mg/ml |
| OBSERVED MW: | 43kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHGH TPRQPTDSPL YPLLSAAAEF YKQALKSHPA RKAAVNYLKG RGLTGEIARD FGLGFAPPGW DNLLKHLGGD NLQLKAMLDA GLLVENSDTG KRYDRFRDRV MFPIRDSRGR IIAFGGRVLG DDKPKYLNSP ETPVFHKGQE LYGLYEARQK NRDLDEIMVV EGYMDVIALA QQGIRNAVAT LGTATSEEHI KRLFRLVPSI LFCFDGDQAG RKAAWRALES VLPNLQDGKR VRFLFLPEGE DPDSLVRAEG EDAFRARITQ QAQPLAEYFF QQLMLEADPA TLEGKAHLAT LAAPLLEKIP GNNLRLLMRQ RLSEITGLSG ENIGQLAHHS PPPSSMDHGA SGVLDGDDYF AASAYYENEP SHAPFDAAPG YVEAQPRKS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGGCACA CGCCGCGCCA GCCGACCGAC TCGCCGCTCT ACCCGCTGCT CAGCGCCGCC GCCGAGTTCT ACAAGCAGGC GCTGAAAAGC CACCCGGCGC GCAAAGCCGC GGTGAACTAC CTGAAGGGCC GCGGCCTGAC CGGCGAGATC GCCCGCGACT TCGGCCTCGG TTTCGCCCCG CCCGGCTGGG ACAACCTGCT CAAGCACCTG GGTGGCGACA ACCTGCAACT CAAGGCCATG CTCGACGCCG GCCTGCTGGT GGAGAACAGC GACACCGGCA AGCGCTACGA CCGCTTCCGC GATCGCGTGA TGTTCCCGAT CCGCGACAGC CGCGGGCGGA TCATCGCCTT CGGCGGCCGG GTGCTCGGCG ACGACAAGCC GAAGTACCTG AACTCCCCGG AGACTCCGGT CTTCCACAAG GGCCAGGAAC TCTACGGACT CTACGAGGCC CGGCAGAAGA ATCGCGACCT CGACGAGATC ATGGTGGTCG AGGGCTACAT GGATGTCATC GCCCTGGCCC AGCAAGGCAT CCGCAACGCG GTGGCGACCC TCGGCACGGC GACCAGCGAG GAACACATCA AGCGCCTGTT CCGCCTGGTG CCGAGCATCC TGTTCTGTTT CGACGGCGAC CAGGCCGGGC GCAAGGCGGC CTGGCGCGCC CTGGAGTCGG TGCTGCCGAA CCTGCAGGAC GGCAAGCGCG TGCGCTTCCT GTTCCTCCCC GAGGGCGAGG ACCCGGACAG CCTGGTACGC GCCGAGGGCG AGGATGCCTT CCGCGCACGC ATCACCCAGC AGGCCCAGCC GCTGGCGGAG TACTTCTTCC AACAACTGAT GCTGGAGGCC GACCCCGCCA CCCTGGAAGG CAAGGCCCAC CTGGCGACCC TCGCCGCGCC GCTGCTGGAG AAGATTCCCG GGAACAACCT GCGCCTGCTG ATGCGGCAAC GTCTGTCGGA AATCACCGGC CTGTCCGGCG AAAACATCGG ACAACTGGCG CACCACAGCC CGCCGCCGTC GTCCATGGAC CACGGAGCGA GCGGCGTCTT GGACGGCGAC GACTATTTCG CCGCCAGCGC CTACTACGAG AACGAGCCCT CCCATGCGCC GTTCGACGCT GCGCCAGGCT ATGTCGAGGC GCAACCGCGC AAGAGCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for PsaeA.00153.a.B2.PS38109
| PURIFICATION DATe: | 11/10/2016 |
| CONCENTRATION: | 26.5mg/ml |
| OBSERVED MW: | 46kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMA GLIPQSFIDD LLNRTDIVEV VSSRIQLKKT GKNYSACCPF HKEKTPSFTV SPDKQFYYCF GCGAGGNALG FVMDHDQLEF PQAVEELAKR AGMDVPREER GGRGHTPRQP TDSPLYPLLS AAAEFYKQAL KSHPARKAAV NYLKGRGLTG EIARDFGLGF APPGWDNLLK HLGGDNLQLK AMLDAGLLVE NSDTGKRYDR FRDRVMFPIR DSRGRIIAFG GRVLGDDKPK YLNSPETPVF HKGQELYGLY EARQKNRDLD EIMVVEGYMD VIALAQQGIR NAVATLGTAT SEEHIKRLFR LVPSILFCFD GDQAGRKAAW RALESVLPNL QDGKRVRFLF LPEGEDPDSL VRAEGEDAFR ARITQQAQPL AEYFFQQLML EADPATLEGK AHLATLAAPL LEKIPGNNLR LLMRQRLSEI TG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCG GCCTGATACC GCAAAGCTTC ATCGATGACC TGCTGAACCG CACCGACATC GTCGAGGTGG TGAGTTCACG CATCCAACTG AAGAAAACCG GCAAGAACTA CAGCGCCTGC TGCCCGTTCC ACAAGGAAAA GACTCCCTCG TTCACCGTCA GCCCGGACAA GCAGTTCTAC TACTGCTTCG GCTGCGGCGC CGGCGGCAAC GCGCTCGGCT TCGTCATGGA CCACGACCAG CTGGAGTTCC CCCAGGCGGT CGAGGAACTG GCCAAGCGCG CCGGCATGGA CGTGCCGCGC GAGGAACGCG GCGGACGCGG GCACACGCCG CGCCAGCCGA CCGACTCGCC GCTCTACCCG CTGCTCAGCG CCGCCGCCGA GTTCTACAAG CAGGCGCTGA AAAGCCACCC GGCGCGCAAA GCCGCGGTGA ACTACCTGAA GGGCCGCGGC CTGACCGGCG AGATCGCCCG CGACTTCGGC CTCGGTTTCG CCCCGCCCGG CTGGGACAAC CTGCTCAAGC ACCTGGGTGG CGACAACCTG CAACTCAAGG CCATGCTCGA CGCCGGCCTG CTGGTGGAGA ACAGCGACAC CGGCAAGCGC TACGACCGCT TCCGCGATCG CGTGATGTTC CCGATCCGCG ACAGCCGCGG GCGGATCATC GCCTTCGGCG GCCGGGTGCT CGGCGACGAC AAGCCGAAGT ACCTGAACTC CCCGGAGACT CCGGTCTTCC ACAAGGGCCA GGAACTCTAC GGACTCTACG AGGCCCGGCA GAAGAATCGC GACCTCGACG AGATCATGGT GGTCGAGGGC TACATGGATG TCATCGCCCT GGCCCAGCAA GGCATCCGCA ACGCGGTGGC GACCCTCGGC ACGGCGACCA GCGAGGAACA CATCAAGCGC CTGTTCCGCC TGGTGCCGAG CATCCTGTTC TGTTTCGACG GCGACCAGGC CGGGCGCAAG GCGGCCTGGC GCGCCCTGGA GTCGGTGCTG CCGAACCTGC AGGACGGCAA GCGCGTGCGC TTCCTGTTCC TCCCCGAGGG CGAGGACCCG GACAGCCTGG TACGCGCCGA GGGCGAGGAT GCCTTCCGCG CACGCATCAC CCAGCAGGCC CAGCCGCTGG CGGAGTACTT CTTCCAACAA CTGATGCTGG AGGCCGACCC CGCCACCCTG GAAGGCAAGG CCCACCTGGC GACCCTCGCC GCGCCGCTGC TGGAGAAGAT TCCCGGGAAC AACCTGCGCC TGCTGATGCG GCAACGTCTG TCGGAAATCA CCGGCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT
Details for PsaeA.00153.a.B2.PS38110
| PURIFICATION DATe: | 11/10/2016 |
| CONCENTRATION: | 25.5mg/ml |
| OBSERVED MW: | 46kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMA GLIPQSFIDD LLNRTDIVEV VSSRIQLKKT GKNYSACCPF HKEKTPSFTV SPDKQFYYCF GCGAGGNALG FVMDHDQLEF PQAVEELAKR AGMDVPREER GGRGHTPRQP TDSPLYPLLS AAAEFYKQAL KSHPARKAAV NYLKGRGLTG EIARDFGLGF APPGWDNLLK HLGGDNLQLK AMLDAGLLVE NSDTGKRYDR FRDRVMFPIR DSRGRIIAFG GRVLGDDKPK YLNSPETPVF HKGQELYGLY EARQKNRDLD EIMVVEGYMD VIALAQQGIR NAVATLGTAT SEEHIKRLFR LVPSILFCFD GDQAGRKAAW RALESVLPNL QDGKRVRFLF LPEGEDPDSL VRAEGEDAFR ARITQQAQPL AEYFFQQLML EADPATLEGK AHLATLAAPL LEKIPGNNLR LLMRQRLSEI TG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCG GCCTGATACC GCAAAGCTTC ATCGATGACC TGCTGAACCG CACCGACATC GTCGAGGTGG TGAGTTCACG CATCCAACTG AAGAAAACCG GCAAGAACTA CAGCGCCTGC TGCCCGTTCC ACAAGGAAAA GACTCCCTCG TTCACCGTCA GCCCGGACAA GCAGTTCTAC TACTGCTTCG GCTGCGGCGC CGGCGGCAAC GCGCTCGGCT TCGTCATGGA CCACGACCAG CTGGAGTTCC CCCAGGCGGT CGAGGAACTG GCCAAGCGCG CCGGCATGGA CGTGCCGCGC GAGGAACGCG GCGGACGCGG GCACACGCCG CGCCAGCCGA CCGACTCGCC GCTCTACCCG CTGCTCAGCG CCGCCGCCGA GTTCTACAAG CAGGCGCTGA AAAGCCACCC GGCGCGCAAA GCCGCGGTGA ACTACCTGAA GGGCCGCGGC CTGACCGGCG AGATCGCCCG CGACTTCGGC CTCGGTTTCG CCCCGCCCGG CTGGGACAAC CTGCTCAAGC ACCTGGGTGG CGACAACCTG CAACTCAAGG CCATGCTCGA CGCCGGCCTG CTGGTGGAGA ACAGCGACAC CGGCAAGCGC TACGACCGCT TCCGCGATCG CGTGATGTTC CCGATCCGCG ACAGCCGCGG GCGGATCATC GCCTTCGGCG GCCGGGTGCT CGGCGACGAC AAGCCGAAGT ACCTGAACTC CCCGGAGACT CCGGTCTTCC ACAAGGGCCA GGAACTCTAC GGACTCTACG AGGCCCGGCA GAAGAATCGC GACCTCGACG AGATCATGGT GGTCGAGGGC TACATGGATG TCATCGCCCT GGCCCAGCAA GGCATCCGCA ACGCGGTGGC GACCCTCGGC ACGGCGACCA GCGAGGAACA CATCAAGCGC CTGTTCCGCC TGGTGCCGAG CATCCTGTTC TGTTTCGACG GCGACCAGGC CGGGCGCAAG GCGGCCTGGC GCGCCCTGGA GTCGGTGCTG CCGAACCTGC AGGACGGCAA GCGCGTGCGC TTCCTGTTCC TCCCCGAGGG CGAGGACCCG GACAGCCTGG TACGCGCCGA GGGCGAGGAT GCCTTCCGCG CACGCATCAC CCAGCAGGCC CAGCCGCTGG CGGAGTACTT CTTCCAACAA CTGATGCTGG AGGCCGACCC CGCCACCCTG GAAGGCAAGG CCCACCTGGC GACCCTCGCC GCGCCGCTGC TGGAGAAGAT TCCCGGGAAC AACCTGCGCC TGCTGATGCG GCAACGTCTG TCGGAAATCA CCGGCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT
Details for PsaeA.00153.a.B4.PS38112
| PURIFICATION DATe: | 11/9/2016 |
| CONCENTRATION: | 25.8mg/ml |
| OBSERVED MW: | 55kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMA GLIPQSFIDD LLNRTDIVEV VSSRIQLKKT GKNYSACCPF HKEKTPSFTV SPDKQFYYCF GCGAGGNALG FVMDHDQLEF PQAVEELAKR AGMDVPREER GGRGHTPRQP TDSPLYPLLS AAAEFYKQAL KSHPARKAAV NYLKGRGLTG EIARDFGLGF APPGWDNLLK HLGGDNLQLK AMLDAGLLVE NSDTGKRYDR FRDRVMFPIR DSRGRIIAFG GRVLGDDKPK YLNSPETPVF HKGQELYGLY EARQKNRDLD EIMVVEGYMD VIALAQQGIR NAVATLGTAT SEEHIKRLFR LVPSILFCFD GDQAGRKAAW RALESVLPNL QDGKRVRFLF LPEGEDPDSL VRAEGEDAFR ARITQQAQPL AEYFFQQLML EADPATLEGK AHLATLAAPL LEKIPGNNLR LLMRQRLSEI TGLSGENIGQ LAHHSPPPSS MDHGASGVLD GDDYFAASAY YENEPSHAPF DAAPGYVEAQ PRKS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCG GCCTGATACC GCAAAGCTTC ATCGATGACC TGCTGAACCG CACCGACATC GTCGAGGTGG TGAGTTCACG CATCCAACTG AAGAAAACCG GCAAGAACTA CAGCGCCTGC TGCCCGTTCC ACAAGGAAAA GACTCCCTCG TTCACCGTCA GCCCGGACAA GCAGTTCTAC TACTGCTTCG GCTGCGGCGC CGGCGGCAAC GCGCTCGGCT TCGTCATGGA CCACGACCAG CTGGAGTTCC CCCAGGCGGT CGAGGAACTG GCCAAGCGCG CCGGCATGGA CGTGCCGCGC GAGGAACGCG GCGGACGCGG GCACACGCCG CGCCAGCCGA CCGACTCGCC GCTCTACCCG CTGCTCAGCG CCGCCGCCGA GTTCTACAAG CAGGCGCTGA AAAGCCACCC GGCGCGCAAA GCCGCGGTGA ACTACCTGAA GGGCCGCGGC CTGACCGGCG AGATCGCCCG CGACTTCGGC CTCGGTTTCG CCCCGCCCGG CTGGGACAAC CTGCTCAAGC ACCTGGGTGG CGACAACCTG CAACTCAAGG CCATGCTCGA CGCCGGCCTG CTGGTGGAGA ACAGCGACAC CGGCAAGCGC TACGACCGCT TCCGCGATCG CGTGATGTTC CCGATCCGCG ACAGCCGCGG GCGGATCATC GCCTTCGGCG GCCGGGTGCT CGGCGACGAC AAGCCGAAGT ACCTGAACTC CCCGGAGACT CCGGTCTTCC ACAAGGGCCA GGAACTCTAC GGACTCTACG AGGCCCGGCA GAAGAATCGC GACCTCGACG AGATCATGGT GGTCGAGGGC TACATGGATG TCATCGCCCT GGCCCAGCAA GGCATCCGCA ACGCGGTGGC GACCCTCGGC ACGGCGACCA GCGAGGAACA CATCAAGCGC CTGTTCCGCC TGGTGCCGAG CATCCTGTTC TGTTTCGACG GCGACCAGGC CGGGCGCAAG GCGGCCTGGC GCGCCCTGGA GTCGGTGCTG CCGAACCTGC AGGACGGCAA GCGCGTGCGC TTCCTGTTCC TCCCCGAGGG CGAGGACCCG GACAGCCTGG TACGCGCCGA GGGCGAGGAT GCCTTCCGCG CACGCATCAC CCAGCAGGCC CAGCCGCTGG CGGAGTACTT CTTCCAACAA CTGATGCTGG AGGCCGACCC CGCCACCCTG GAAGGCAAGG CCCACCTGGC GACCCTCGCC GCGCCGCTGC TGGAGAAGAT TCCCGGGAAC AACCTGCGCC TGCTGATGCG GCAACGTCTG TCGGAAATCA CCGGCCTGTC CGGCGAAAAC ATCGGACAAC TGGCGCACCA CAGCCCGCCG CCGTCGTCCA TGGACCACGG AGCGAGCGGC GTCTTGGACG GCGACGACTA TTTCGCCGCC AGCGCCTACT ACGAGAACGA GCCCTCCCAT GCGCCGTTCG ACGCTGCGCC AGGCTATGTC GAGGCGCAAC CGCGCAAGAG CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT