PsaeA.00149.a

Ubiquinone biosynthesis O-methyltransferase (2-polyprenyl-6-hydroxyphenol methylase) (EC 2.1.1.222) (3-demethylubiquinone 3-O-methyltransferase) (EC 2.1.1.64)

CENTER ID: PsaeA.00149.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00149.a.B1.GE38206 full length 1 232
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.3314
PGAT: pseudomonas:PA3171
RefSeq: NP_251861.1
UniProt: Q9HZ63

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNVDHAEIA KFEALAHRWW DRESEFKPLH DINPLRVNWI DERAGLAGKK VLDIGCGGGI LSEAMAQRGA SVTGIDMGEA PLAVARLHQL ESGVAVDYRQ ITAEQMAEEM PGQFDVVTCL EMLEHVPDPA SVIRACHRLV KPGGQVFLST INRNPKAYLF AVIGAEYILQ LLPRGTHDFR KFIRPSELGA WSREAGLEVK DIIGLTYNPL TKHYKLANDV DVNYMVQTKR EA
NT Sequence
atgagcaacg tcgaccacgc cgagatcgcc aaattcgagg cactggccca tcgctggtgg gaccgcgaga gcgagttcaa gccgctccac gacatcaacc cgctgcgcgt caactggatc gacgagcgcg ccggcctggc cggcaagaag gtgctcgaca tcggttgcgg cggcggcatc ctcagcgaag ccatggccca gcgcggcgcc agcgtcaccg gcatcgacat gggcgaagcg ccgctcgcgg tggcgcgcct gcaccagctg gaatccggcg tcgccgtcga ctaccggcag atcaccgccg agcagatggc cgaggaaatg cccgggcaat tcgacgtggt cacctgcctg gagatgctcg aacacgtgcc ggacccggcc tcggtgatcc gcgcctgcca ccgcctggtc aagccgggcg gacaggtatt cctctcgacc atcaaccgca atcccaaggc ctacctgttc gcggtgatcg gcgcggaata catcctccag ttgctgccgc gcgggaccca cgatttccgc aagttcattc gtccgtcgga actcggcgcc tggtcgcgcg aggccggtct cgaggtcaag gacatcatcg gcctgaccta caacccgctg accaagcact acaagctggc caacgacgtc gacgtcaact acatggtcca gaccaagcgg gaggcgtga
Details for PsaeA.00149.a.B1.GE38206
HARVESTED ON: 6/11/2014
SEQUENCED ON: 6/12/2014
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS NVDHAEIAKF EALAHRWWDR ESEFKPLHDI NPLRVNWIDE RAGLAGKKVL DIGCGGGILS EAMAQRGASV TGIDMGEAPL AVARLHQLES GVAVDYRQIT AEQMAEEMPG QFDVVTCLEM LEHVPDPASV IRACHRLVKP GGQVFLSTIN RNPKAYLFAV IGAEYILQLL PRGTHDFRKF IRPSELGAWS REAGLEVKDI IGLTYNPLTK HYKLANDVDV NYMVQTKREA
Validated NT Sequence
atcttactca cttacgcctc ccgcttggtc tggaccatgt agttgacgtc gacgtcgttg gccagcttgt agtgcttggt cagcgggttg taggtcaggc cgatgatgtc cttgacctcg agaccggcct cgcgcgacca ggcgccgagt tccgacggac gaatgaactt gcggaaatcg tgggtcccgc gcggcagcaa ctggaggatg tattccgcgc cgatcaccgc gaacaggtag gccttgggat tgcggttgat ggtcgagagg aatacctgtc cgcccggctt gaccaggcgg tggcaggcgc ggatcaccga ggccgggtcc ggcacgtgtt cgagcatctc caggcaggtg accacgtcga attgcccggg catttcctcg gccatctgct cggcggtgat ctgccggtag tcgacggcga cgccggattc cagctggtgc aggcgcgcca ccgcgagcgg cgcttcgccc atgtcgatgc cggtgacgct ggcgccgcgc tgggccatgg cttcgctgag gatgccgccg ccgcaaccga tgtcgagcac cttcttgccg gccaggccgg cgcgctcgtc gatccagttg acgcgcagcg ggttgatgtc gtggagcggc ttgaactcgc tctcgcggtc ccaccagcga tgggccagtg cctcgaattt ggcgatctcg gcgtggtcga cgttgctcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMS NVDHAEIAKF EALAHRWWDR ESEFKPLHDI NPLRVNWIDE RAGLAGKKVL DIGCGGGILS EAMAQRGASV TGIDMGEAPL AVARLHQLES GVAVDYRQIT AEQMAEEMPG QFDVVTCLEM LEHVPDPASV IRACHRLVKP GGQVFLSTIN RNPKAYLFAV IGAEYILQLL PRGTHDFRKF IRPSELGAWS REAGLEVKDI IGLTYNPLTK HYKLANDVDV NYMVQTKREA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca acgtcgacca cgccgagatc gccaaattcg aggcactggc ccatcgctgg tgggaccgcg agagcgagtt caagccgctc cacgacatca acccgctgcg cgtcaactgg atcgacgagc gcgccggcct ggccggcaag aaggtgctcg acatcggttg cggcggcggc atcctcagcg aagccatggc ccagcgcggc gccagcgtca ccggcatcga catgggcgaa gcgccgctcg cggtggcgcg cctgcaccag ctggaatccg gcgtcgccgt cgactaccgg cagatcaccg ccgagcagat ggccgaggaa atgcccgggc aattcgacgt ggtcacctgc ctggagatgc tcgaacacgt gccggacccg gcctcggtga tccgcgcctg ccaccgcctg gtcaagccgg gcggacaggt attcctctcg accatcaacc gcaatcccaa ggcctacctg ttcgcggtga tcggcgcgga atacatcctc cagttgctgc cgcgcgggac ccacgatttc cgcaagttca ttcgtccgtc ggaactcggc gcctggtcgc gcgaggccgg tctcgaggtc aaggacatca tcggcctgac ctacaacccg ctgaccaagc actacaagct ggccaacgac gtcgacgtca actacatggt ccagaccaag cgggaggcgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat