PsaeA.00139.a

Dephospho-CoA kinase (EC 2.7.1.24) (Dephosphocoenzyme A kinase)

CENTER ID: PsaeA.00139.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00139.a.B1.GE41129 full length 1 203
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00139.a.B1.PW38233 full length 1 203

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4740
PGAT: pseudomonas:PA4529
UniProt: Q9HVP8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQPWILGLT GGIGSGKSAA AEHFISLGVH LVDADHAARW VVEPGRPALA KIVERFGDGI LLPDGQLDRA ALRERIFQAP EERRWLEQLL HPLIGAEIVQ YLARAESPYA ILVSPLLVES GQRQMTHRVL VVDTPEHLQL QRTMLRDKVS EEQVRSILQA QARRDERLKH ADDVLVNDGD LSHLQREVER LHAFYLTLRG GRA
NT Sequence
atgacccaac cctggatact aggcctcacg ggcggtatcg gcagcggcaa gagcgcagcc gccgagcact tcatctccct cggcgtccac ctggtggacg ccgaccatgc cgcccgctgg gtggtggagc cggggcgtcc ggccctggcg aagatcgtcg aacgcttcgg cgacggcatc ctcctgcccg atggccagct cgaccgcgcc gccttgcgcg agcggatctt ccaggcgccg gaggaacggc gctggctgga gcaattgctg cacccgctga tcggcgcgga gatcgtccag tacctggcca gggccgaatc gccctatgcc atcctggtct cgccgctgct ggtggagtcc ggccagcgcc agatgaccca ccgcgtgctg gtggtggata ccccggaaca tctgcaacta cagcgcacca tgcttcgcga caaggtctcc gaggagcagg tgcgctcgat cctccaggcc caggcccggc gcgacgagcg cttgaagcac gccgacgacg tgctggtcaa cgacggcgac ctgtcccacc tgcagcgtga agtcgagcga ctgcacgcct tctacctgac cttgcgagga ggccgagcat ga
Details for PsaeA.00139.a.B1.GE41129
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 24kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT QPWILGLTGG IGSGKSAAAE HFISLGVHLV DADHAARWVV EPGRPALAKI VERFGDGILL PDGQLDRAAL RERIFQAPEE RRWLEQLLHP LIGAEIVQYL ARAESPYAIL VSPLLVESGQ RQMTHRVLVV DTPEHLQLQR TMLRDKVSEE QVRSILQAQA RRDERLKHAD DVLVNDGDLS HLQREVERLH AFYLTLRGGR A
Validated NT Sequence
atcttactca cttatgctcg gcctcctcgc aaggtcaggt agaaggcgtg cagtcgctcg acttcacgct gcaggtggga caggtcgccg tcgttgacca gcacgtcgtc ggcgtgcttc aagcgctcgt cgcgccgggc ctgggcctgg aggatcgagc gcacctgctc ctcggagacc ttgtcgcgaa gcatggtgcg ctgtagttgc agatgttccg gggtatccac caccagcacg cggtgggtca tctggcgctg gccggactcc accagcagcg gcgagaccag gatggcatag ggcgattcgg ccctggccag gtactggacg atctccgcgc cgatcagcgg gtgcagcaat tgctccagcc agcgccgttc ctccggcgcc tggaagatcc gctcgcgcaa ggcggcgcgg tcgagctggc catcgggcag gaggatgccg tcgccgaagc gttcgacgat cttcgccagg gccggacgcc ccggctccac cacccagcgg gcggcatggt cggcgtccac caggtggacg ccgagggaga tgaagtgctc ggcggctgcg ctcttgccgc tgccgatacc gcccgtgagg cccagtatcc agggttgggt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMT QPWILGLTGG IGSGKSAAAE HFISLGVHLV DADHAARWVV EPGRPALAKI VERFGDGILL PDGQLDRAAL RERIFQAPEE RRWLEQLLHP LIGAEIVQYL ARAESPYAIL VSPLLVESGQ RQMTHRVLVV DTPEHLQLQR TMLRDKVSEE QVRSILQAQA RRDERLKHAD DVLVNDGDLS HLQREVERLH AFYLTLRGGR A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccc aaccctggat actaggcctc acgggcggta tcggcagcgg caagagcgca gccgccgagc acttcatctc cctcggcgtc cacctggtgg acgccgacca tgccgcccgc tgggtggtgg agccggggcg tccggccctg gcgaagatcg tcgaacgctt cggcgacggc atcctcctgc ccgatggcca gctcgaccgc gccgccttgc gcgagcggat cttccaggcg ccggaggaac ggcgctggct ggagcaattg ctgcacccgc tgatcggcgc ggagatcgtc cagtacctgg ccagggccga atcgccctat gccatcctgg tctcgccgct gctggtggag tccggccagc gccagatgac ccaccgcgtg ctggtggtgg ataccccgga acatctgcaa ctacagcgca ccatgcttcg cgacaaggtc tccgaggagc aggtgcgctc gatcctccag gcccaggccc ggcgcgacga gcgcttgaag cacgccgacg acgtgctggt caacgacggc gacctgtccc acctgcagcg tgaagtcgag cgactgcacg ccttctacct gaccttgcga ggaggccgag catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for PsaeA.00139.a.B1.PW38233
PURIFICATION DATe: 3/27/2017
CONCENTRATION: 30mg/ml
OBSERVED MW: 25kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 8.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT QPWILGLTGG IGSGKSAAAE HFISLGVHLV DADHAARWVV EPGRPALAKI VERFGDGILL PDGQLDRAAL RERIFQAPEE RRWLEQLLHP LIGAEIVQYL ARAESPYAIL VSPLLVESGQ RQMTHRVLVV DTPEHLQLQR TMLRDKVSEE QVRSILQAQA RRDERLKHAD DVLVNDGDLS HLQREVERLH AFYLTLRGGR A
Validated NT Sequence
atcttactca cttatgctcg gcctcctcgc aaggtcaggt agaaggcgtg cagtcgctcg acttcacgct gcaggtggga caggtcgccg tcgttgacca gcacgtcgtc ggcgtgcttc aagcgctcgt cgcgccgggc ctgggcctgg aggatcgagc gcacctgctc ctcggagacc ttgtcgcgaa gcatggtgcg ctgtagttgc agatgttccg gggtatccac caccagcacg cggtgggtca tctggcgctg gccggactcc accagcagcg gcgagaccag gatggcatag ggcgattcgg ccctggccag gtactggacg atctccgcgc cgatcagcgg gtgcagcaat tgctccagcc agcgccgttc ctccggcgcc tggaagatcc gctcgcgcaa ggcggcgcgg tcgagctggc catcgggcag gaggatgccg tcgccgaagc gttcgacgat cttcgccagg gccggacgcc ccggctccac cacccagcgg gcggcatggt cggcgtccac caggtggacg ccgagggaga tgaagtgctc ggcggctgcg ctcttgccgc tgccgatacc gcccgtgagg cccagtatcc agggttgggt catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMT QPWILGLTGG IGSGKSAAAE HFISLGVHLV DADHAARWVV EPGRPALAKI VERFGDGILL PDGQLDRAAL RERIFQAPEE RRWLEQLLHP LIGAEIVQYL ARAESPYAIL VSPLLVESGQ RQMTHRVLVV DTPEHLQLQR TMLRDKVSEE QVRSILQAQA RRDERLKHAD DVLVNDGDLS HLQREVERLH AFYLTLRGGR A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCC AACCCTGGAT ACTAGGCCTC ACGGGCGGTA TCGGCAGCGG CAAGAGCGCA GCCGCCGAGC ACTTCATCTC CCTCGGCGTC CACCTGGTGG ACGCCGACCA TGCCGCCCGC TGGGTGGTGG AGCCGGGGCG TCCGGCCCTG GCGAAGATCG TCGAACGCTT CGGCGACGGC ATCCTCCTGC CCGATGGCCA GCTCGACCGC GCCGCCTTGC GCGAGCGGAT CTTCCAGGCG CCGGAGGAAC GGCGCTGGCT GGAGCAATTG CTGCACCCGC TGATCGGCGC GGAGATCGTC CAGTACCTGG CCAGGGCCGA ATCGCCCTAT GCCATCCTGG TCTCGCCGCT GCTGGTGGAG TCCGGCCAGC GCCAGATGAC CCACCGCGTG CTGGTGGTGG ATACCCCGGA ACATCTGCAA CTACAGCGCA CCATGCTTCG CGACAAGGTC TCCGAGGAGC AGGTGCGCTC GATCCTCCAG GCCCAGGCCC GGCGCGACGA GCGCTTGAAG CACGCCGACG ACGTGCTGGT CAACGACGGC GACCTGTCCC ACCTGCAGCG TGAAGTCGAG CGACTGCACG CCTTCTACCT GACCTTGCGA GGAGGCCGAG CATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT