PsaeA.00137.b
UDP-N-acetylmuramate--L-alanine ligase (EC 6.3.2.8) (UDP-N-acetylmuramoyl-L-alanine synthetase)
CENTER ID:
PsaeA.00137.b
ORGANISM:
Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:
Pseudomonas infection
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
unclassified
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
unclassified
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
PsaeA.00137.b.B1.GE38203
full length
info
1
480
PsaeA.00137.b.B1.GE38203
PsaeA.00137.b.B2.GE40416
truncation 1
info
12
471
PsaeA.00137.b.B2.GE40416
PsaeA.00137.b.B3.GE40417
truncation 2
info
12
463
PsaeA.00137.b.B3.GE40417
PsaeA.00137.b.B4.GE40418
truncation 3
info
17
384
PsaeA.00137.b.B4.GE40418
PsaeA.00137.b.B5.GE40419
truncation 4
info
16
322
PsaeA.00137.b.B5.GE40419
PsaeA.00137.b.B6.GE40420
truncation 5
info
39
480
PsaeA.00137.b.B6.GE40420
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
PsaeA.00137.b.B1.PW38298
full length
info
1
480
PW38298
PsaeA.00137.b.B5.PW39258
truncation 4
info
16
322
PW39258
Structures
5VVW
DEPOSITED: 5/21/2017
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW37941
6X9F
DEPOSITED: 6/2/2020
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW37941
6X9N
DEPOSITED: 6/3/2020
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW37941
8DOF
DEPOSITED: 7/13/2022
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW37941
8EGM
DEPOSITED: 9/12/2022
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW37941
8EGN
DEPOSITED: 9/12/2022
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW37941
8EWA
DEPOSITED: 10/21/2022
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW39129
9D9M
DEPOSITED: 8/21/2024
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW37941
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVKEPNGVTR TMRRIRRIHF VGIGGAGMCG IAEVLLNLGY EVSGSDLKAS AVTERLEKFG AQIFIGHQAE NADGADVLVV SSAINRANPE VASALERRIP VVPRAEMLAE LMRYRHGIAV AGTHGKTTTT SLIASVFAAG GLDPTFVIGG RLNAAGTNAQ LGASRYLVAE ADESDASFLH LQPMVAVVTN IDADHMATYG GDFNKLKKTF VEFLHNLPFY GLAVMCVDDP VVREILPQIA RPTVTYGLSE DADVRAINIR QEGMRTWFTV LRPEREPLDV SVNMPGLHNV LNSLATIVIA TDEGISDEAI VQGLSGFQGV GRRFQVYGEL QVEGGSVMLV DDYGHHPREV AAVIKAIRGG WPERRLVMVY QPHRYTRTRD LYEDFVQVLG EANVLLLMEV YPAGEEPIPG ADSRQLCHSI RQRGQLDPIY FERDADLAPL VKPLLRAGDI LLCQGAGDVG GLAPQLIKNP LFAGKGGKGA
NT Sequence
gtggttaaag aaccgaatgg cgtcacccgg accatgcgcc gtatccgccg catccatttc gtcggcatcg gcggcgccgg tatgtgcggg atcgccgaag tgctgctgaa cctcggctac gaggtatccg gctcggacct caaggcctcg gcggtgaccg agcgcctgga gaagttcggc gcgcagatct tcatcggcca ccaggcggaa aacgccgacg gcgccgacgt gctggtggtg tccagtgcca tcaaccgggc caacccggaa gtggcatcgg ccctggaacg gcggattccg gtggtgccgc gtgcggagat gctcgccgag ctgatgcgct accggcacgg catcgcggta gccggcaccc acggcaagac caccactacc agcctgatcg cctcggtgtt cgccgccggc ggcctggacc cgaccttcgt catcggcggc cggctgaacg ccgccgggac caacgcccag ctcggcgcca gccgctacct ggtggccgag gccgacgaga gcgacgccag cttcctgcac ctgcaaccga tggtcgcggt ggtcaccaat atcgacgccg accacatggc gacctacggc ggcgacttca acaagctgaa gaagaccttc gtcgagttcc tccacaacct gccgttctac ggactggcgg tgatgtgcgt ggatgatccg gttgtgcgtg agatcctccc gcagatcgcc cgcccgaccg tgacctacgg cctcagcgaa gacgccgacg tgcgcgcgat caacatccgc caggaaggca tgcgcacctg gttcaccgtg ttgcgcccgg agcgcgagcc gctggacgtc tcggtgaaca tgcccggcct gcacaacgtg ctgaattccc tggcgaccat cgtcatcgct accgacgagg gcatctccga cgaagccatc gtccaggggc tgtccggctt ccagggcgta ggccggcgct tccaggtcta cggcgagctg caggtcgagg gtggcagcgt gatgctggtg gacgattacg gccaccatcc gcgcgaagtc gccgcggtga tcaaggcgat ccgtggcggt tggccggagc gtcgcctggt gatggtctac cagccgcatc gctatacccg tacccgcgac ctgtacgaag acttcgtgca ggtgctgggc gaagccaacg tgctgctgtt gatggaggtc tatccggccg gcgaagagcc gatcccggga gccgacagcc gccagctgtg ccacagcatc cgccagcgcg gccagcttga cccgatctac ttcgagcgcg acgccgacct ggcgccgctg gtcaagccgc tgctgcgcgc tggcgacatc ctgctttgcc agggcgctgg cgatgtcggc ggcctggccc cgcaactgat caagaacccg ctgttcgccg gcaagggagg gaagggcgca tga
Details for PsaeA.00137.b.B1.GE38203
HARVESTED ON:
6/11/2014
SEQUENCED ON:
6/12/2014
EXPECTED MW:
53kDa
OBSERVED MW:
50kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
100
PERCENT COVERAGE:
96
Details for PsaeA.00137.b.B2.GE40416
HARVESTED ON:
7/15/2016
SEQUENCED ON:
7/18/2016
EXPECTED MW:
51kDa
OBSERVED MW:
51kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for PsaeA.00137.b.B3.GE40417
HARVESTED ON:
7/15/2016
SEQUENCED ON:
7/22/2016
EXPECTED MW:
50kDa
OBSERVED MW:
50kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Insoluble
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for PsaeA.00137.b.B4.GE40418
HARVESTED ON:
7/15/2016
SEQUENCED ON:
7/18/2016
EXPECTED MW:
41kDa
OBSERVED MW:
41kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Insoluble
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for PsaeA.00137.b.B5.GE40419
HARVESTED ON:
7/15/2016
SEQUENCED ON:
7/18/2016
EXPECTED MW:
34kDa
OBSERVED MW:
34kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for PsaeA.00137.b.B6.GE40420
HARVESTED ON:
7/15/2016
SEQUENCED ON:
7/18/2016
EXPECTED MW:
49kDa
OBSERVED MW:
49kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Low Expression
SOLUBLE EXPRESSION LEVEL
Insoluble
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for PsaeA.00137.b.B1.PW38298
PURIFICATION DATe:
6/12/2017
CONCENTRATION:
33.2mg/ml
OBSERVED MW:
56kDa
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
4
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
96
Details for PsaeA.00137.b.B5.PW39258
PURIFICATION DATe:
1/30/2024
CONCENTRATION:
16.6mg/ml
OBSERVED MW:
34kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
42
VIAL VOLUME:
200µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable