PsaeA.00137.b
UDP-N-acetylmuramate--L-alanine ligase (EC 6.3.2.8) (UDP-N-acetylmuramoyl-L-alanine synthetase)
| CENTER ID: | PsaeA.00137.b |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00137.b.B1.GE38203 | full length | 1 | 480 | ||
| PsaeA.00137.b.B2.GE40416 | truncation 1 | 12 | 471 | ||
| PsaeA.00137.b.B3.GE40417 | truncation 2 | 12 | 463 | ||
| PsaeA.00137.b.B4.GE40418 | truncation 3 | 17 | 384 | ||
| PsaeA.00137.b.B5.GE40419 | truncation 4 | 16 | 322 | ||
| PsaeA.00137.b.B6.GE40420 | truncation 5 | 39 | 480 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00137.b.B1.PW38298 | full length | 1 | 480 |
Structures
5VVW
DEPOSITED: 5/21/2017
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW37941
6X9F
DEPOSITED: 6/2/2020
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW37941
6X9N
DEPOSITED: 6/3/2020
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW37941
8DOF
DEPOSITED: 7/13/2022
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW37941
8EGM
DEPOSITED: 9/12/2022
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW37941
8EGN
DEPOSITED: 9/12/2022
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW37941
8EWA
DEPOSITED: 10/21/2022
DETERMINATION: XRay
CLONE: PsaeA.00137.b.B5.GE40419
PROTEIN: PsaeA.00137.b.B5.PW39129
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.4620 |
| PGAT: | pseudomonas:PA4411 |
| RefSeq: | NP_253101.1 |
| UniProt: | Q9HW02 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVKEPNGVTR TMRRIRRIHF VGIGGAGMCG IAEVLLNLGY EVSGSDLKAS AVTERLEKFG AQIFIGHQAE NADGADVLVV SSAINRANPE VASALERRIP VVPRAEMLAE LMRYRHGIAV AGTHGKTTTT SLIASVFAAG GLDPTFVIGG RLNAAGTNAQ LGASRYLVAE ADESDASFLH LQPMVAVVTN IDADHMATYG GDFNKLKKTF VEFLHNLPFY GLAVMCVDDP VVREILPQIA RPTVTYGLSE DADVRAINIR QEGMRTWFTV LRPEREPLDV SVNMPGLHNV LNSLATIVIA TDEGISDEAI VQGLSGFQGV GRRFQVYGEL QVEGGSVMLV DDYGHHPREV AAVIKAIRGG WPERRLVMVY QPHRYTRTRD LYEDFVQVLG EANVLLLMEV YPAGEEPIPG ADSRQLCHSI RQRGQLDPIY FERDADLAPL VKPLLRAGDI LLCQGAGDVG GLAPQLIKNP LFAGKGGKGA
NT Sequence
gtggttaaag aaccgaatgg cgtcacccgg accatgcgcc gtatccgccg catccatttc gtcggcatcg gcggcgccgg tatgtgcggg atcgccgaag tgctgctgaa cctcggctac gaggtatccg gctcggacct caaggcctcg gcggtgaccg agcgcctgga gaagttcggc gcgcagatct tcatcggcca ccaggcggaa aacgccgacg gcgccgacgt gctggtggtg tccagtgcca tcaaccgggc caacccggaa gtggcatcgg ccctggaacg gcggattccg gtggtgccgc gtgcggagat gctcgccgag ctgatgcgct accggcacgg catcgcggta gccggcaccc acggcaagac caccactacc agcctgatcg cctcggtgtt cgccgccggc ggcctggacc cgaccttcgt catcggcggc cggctgaacg ccgccgggac caacgcccag ctcggcgcca gccgctacct ggtggccgag gccgacgaga gcgacgccag cttcctgcac ctgcaaccga tggtcgcggt ggtcaccaat atcgacgccg accacatggc gacctacggc ggcgacttca acaagctgaa gaagaccttc gtcgagttcc tccacaacct gccgttctac ggactggcgg tgatgtgcgt ggatgatccg gttgtgcgtg agatcctccc gcagatcgcc cgcccgaccg tgacctacgg cctcagcgaa gacgccgacg tgcgcgcgat caacatccgc caggaaggca tgcgcacctg gttcaccgtg ttgcgcccgg agcgcgagcc gctggacgtc tcggtgaaca tgcccggcct gcacaacgtg ctgaattccc tggcgaccat cgtcatcgct accgacgagg gcatctccga cgaagccatc gtccaggggc tgtccggctt ccagggcgta ggccggcgct tccaggtcta cggcgagctg caggtcgagg gtggcagcgt gatgctggtg gacgattacg gccaccatcc gcgcgaagtc gccgcggtga tcaaggcgat ccgtggcggt tggccggagc gtcgcctggt gatggtctac cagccgcatc gctatacccg tacccgcgac ctgtacgaag acttcgtgca ggtgctgggc gaagccaacg tgctgctgtt gatggaggtc tatccggccg gcgaagagcc gatcccggga gccgacagcc gccagctgtg ccacagcatc cgccagcgcg gccagcttga cccgatctac ttcgagcgcg acgccgacct ggcgccgctg gtcaagccgc tgctgcgcgc tggcgacatc ctgctttgcc agggcgctgg cgatgtcggc ggcctggccc cgcaactgat caagaacccg ctgttcgccg gcaagggagg gaagggcgca tga
Details for PsaeA.00137.b.B1.GE38203
| HARVESTED ON: | 6/11/2014 |
| SEQUENCED ON: | 6/12/2014 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MRRIRRIHFV GIGGAGMCGI AEVLLNLGYE VSGSDLKASA VTERLEKFGA QIFIGHQAEN ADGADVLVVS SAINRANPEV ASALERRIPV VPRAEMLAEL MRYRHGIAVA GTHGKTTTTS LIASVFAAGG LDPTFVIGGR LNAAGTNAQL GASRYLVAEA DESDASFLHL QPMVAVVTNI DADHMATYGG DFNKLKKTFV EFLHNLPFYG LAVMCVDDPV VREILPQIAR PTVTYGLSED ADVRAINIRQ EGMRTWFTVL RPEREPLDVS VNMPGLHNVL NSLATIVIAT DEGISDEAIV QGLSGFQGVG RRFQVYGELQ VEGGSVMLVD DYGHHPREVA AVIKAIRGGW PERRLVMVYQ PHRYTRTRDL YEDFVQVLGE ANVLLLMEVY PAGEEPIPGA DSRQLCHSIR QRGQLDPIYF ERDADLAPLV KPLLRAGDIL LCQGAGDVGG LAPQLIKNPL FAGKGGKGA
Validated NT Sequence
atcttactca cttatgcgcc cttccctccc ttgccggcga acagcgggtt cttgatcagt tgcggggcca ggccgccgac atcgccagcg ccctggcaaa gcaggatgtc gccagcgcgc agcagcggct tgaccagcgg cgccaggtcg gcgtcgcgct cgaagtagat cgggtcaagc tggccgcgct ggcggatgct gtggcacagc tggcggctgt cggctcccgg gatcggctct tcgccggccg gatagacctc catcaacagc agcacgttgg cttcgcccag cacctgcacg aagtcttcgt acaggtcgcg ggtacgggta tagcgatgcg gctggtagac catcaccagg cgacgctccg gccaaccgcc acggatcgcc ttgatcaccg cggcgacttc gcgcggatgg tggccgtaat cgtccaccag catcacgctg ccaccctcga cctgcagctc gccgtagacc tggaagcgcc ggcctacgcc ctggaagccg gacagcccct ggacgatggc ttcgtcggag atgccctcgt cggtagcgat gacgatggtc gccagggaat tcagcacgtt gtgcaggccg ggcatgttca ccgagacgtc cagcggctcg cgctccgggc gcaacacggt gaaccaggtg cgcatgcctt cctggcggat gttgatcgcg cgcacgtcgg cgtcttcgct gaggccgtag gtcacggtcg ggcgggcgat ctgcgggagg atctcacgca caaccggatc atccacgcac atcaccgcca gtccgtagaa cggcaggttg tggaggaact cgacgaaggt cttcttcagc ttgttgaagt cgccgccgta ggtcgccatg tggtcggcgt cgatattggt gaccaccgcg accatcggtt gcaggtgcag gaagctggcg tcgctctcgt cggcctcggc caccaggtag cggctggcgc cgagctgggc gttggtcccg gcggcgttca gccggccgcc gatgacgaag gtcgggtcca ggccgccggc ggcgaacacc gaggcgatca ggctggtagt ggtggtcttg ccgtgggtgc cggctaccgc gatgccgtgc cggtagcgca tcagctcggc gagcatctcc gcacgcggca ccaccggaat ccgccgttcc agggccgatg ccacttccgg gttggcccgg ttgatggcac tggacaccac cagcacgtcg gcgccgtcgg cgttttccgc ctggtggccg atgaagatct gcgcgccgaa cttctccagg cgctcggtca ccgccgaggc cttgaggtcc gagccggata cctcgtagcc gaggttcagc agcacttcgg cgatcccgca cataccggcg ccgccgatgc cgacgaaatg gatgcggcgg atacggcgca tggtccgggt gacgccattc ggttcttta
Expected Protein Sequence
MAHHHHHHMV KEPNGVTRTM RRIRRIHFVG IGGAGMCGIA EVLLNLGYEV SGSDLKASAV TERLEKFGAQ IFIGHQAENA DGADVLVVSS AINRANPEVA SALERRIPVV PRAEMLAELM RYRHGIAVAG THGKTTTTSL IASVFAAGGL DPTFVIGGRL NAAGTNAQLG ASRYLVAEAD ESDASFLHLQ PMVAVVTNID ADHMATYGGD FNKLKKTFVE FLHNLPFYGL AVMCVDDPVV REILPQIARP TVTYGLSEDA DVRAINIRQE GMRTWFTVLR PEREPLDVSV NMPGLHNVLN SLATIVIATD EGISDEAIVQ GLSGFQGVGR RFQVYGELQV EGGSVMLVDD YGHHPREVAA VIKAIRGGWP ERRLVMVYQP HRYTRTRDLY EDFVQVLGEA NVLLLMEVYP AGEEPIPGAD SRQLCHSIRQ RGQLDPIYFE RDADLAPLVK PLLRAGDILL CQGAGDVGGL APQLIKNPLF AGKGGKGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggtta aagaaccgaa tggcgtcacc cggaccatgc gccgtatccg ccgcatccat ttcgtcggca tcggcggcgc cggtatgtgc gggatcgccg aagtgctgct gaacctcggc tacgaggtat ccggctcgga cctcaaggcc tcggcggtga ccgagcgcct ggagaagttc ggcgcgcaga tcttcatcgg ccaccaggcg gaaaacgccg acggcgccga cgtgctggtg gtgtccagtg ccatcaaccg ggccaacccg gaagtggcat cggccctgga acggcggatt ccggtggtgc cgcgtgcgga gatgctcgcc gagctgatgc gctaccggca cggcatcgcg gtagccggca cccacggcaa gaccaccact accagcctga tcgcctcggt gttcgccgcc ggcggcctgg acccgacctt cgtcatcggc ggccggctga acgccgccgg gaccaacgcc cagctcggcg ccagccgcta cctggtggcc gaggccgacg agagcgacgc cagcttcctg cacctgcaac cgatggtcgc ggtggtcacc aatatcgacg ccgaccacat ggcgacctac ggcggcgact tcaacaagct gaagaagacc ttcgtcgagt tcctccacaa cctgccgttc tacggactgg cggtgatgtg cgtggatgat ccggttgtgc gtgagatcct cccgcagatc gcccgcccga ccgtgaccta cggcctcagc gaagacgccg acgtgcgcgc gatcaacatc cgccaggaag gcatgcgcac ctggttcacc gtgttgcgcc cggagcgcga gccgctggac gtctcggtga acatgcccgg cctgcacaac gtgctgaatt ccctggcgac catcgtcatc gctaccgacg agggcatctc cgacgaagcc atcgtccagg ggctgtccgg cttccagggc gtaggccggc gcttccaggt ctacggcgag ctgcaggtcg agggtggcag cgtgatgctg gtggacgatt acggccacca tccgcgcgaa gtcgccgcgg tgatcaaggc gatccgtggc ggttggccgg agcgtcgcct ggtgatggtc taccagccgc atcgctatac ccgtacccgc gacctgtacg aagacttcgt gcaggtgctg ggcgaagcca acgtgctgct gttgatggag gtctatccgg ccggcgaaga gccgatcccg ggagccgaca gccgccagct gtgccacagc atccgccagc gcggccagct tgacccgatc tacttcgagc gcgacgccga cctggcgccg ctggtcaagc cgctgctgcg cgctggcgac atcctgcttt gccagggcgc tggcgatgtc ggcggcctgg ccccgcaact gatcaagaac ccgctgttcg ccggcaaggg agggaagggc gcatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PsaeA.00137.b.B2.GE40416
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 51kDa |
| OBSERVED MW: | 51kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMR RIRRIHFVGI GGAGMCGIAE VLLNLGYEVS GSDLKASAVT ERLEKFGAQI FIGHQAENAD GADVLVVSSA INRANPEVAS ALERRIPVVP RAEMLAELMR YRHGIAVAGT HGKTTTTSLI ASVFAAGGLD PTFVIGGRLN AAGTNAQLGA SRYLVAEADE SDASFLHLQP MVAVVTNIDA DHMATYGGDF NKLKKTFVEF LHNLPFYGLA VMCVDDPVVR EILPQIARPT VTYGLSEDAD VRAINIRQEG MRTWFTVLRP EREPLDVSVN MPGLHNVLNS LATIVIATDE GISDEAIVQG LSGFQGVGRR FQVYGELQVE GGSVMLVDDY GHHPREVAAV IKAIRGGWPE RRLVMVYQPH RYTRTRDLYE DFVQVLGEAN VLLLMEVYPA GEEPIPGADS RQLCHSIRQR GQLDPIYFER DADLAPLVKP LLRAGDILLC QGAGDVGGLA PQLIKNPL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcc gtatccgccg catccatttc gtcggcatcg gcggcgccgg tatgtgcggg atcgccgaag tgctgctgaa cctcggctac gaggtatccg gctcggacct caaggcctcg gcggtgaccg agcgcctgga gaagttcggc gcgcagatct tcatcggcca ccaggcggaa aacgccgacg gcgccgacgt gctggtggtg tccagtgcca tcaaccgggc caacccggaa gtggcatcgg ccctggaacg gcggattccg gtggtgccgc gtgcggagat gctcgccgag ctgatgcgct accggcacgg catcgcggta gccggcaccc acggcaagac caccactacc agcctgatcg cctcggtgtt cgccgccggc ggcctggacc cgaccttcgt catcggcggc cggctgaacg ccgccgggac caacgcccag ctcggcgcca gccgctacct ggtggccgag gccgacgaga gcgacgccag cttcctgcac ctgcaaccga tggtcgcggt ggtcaccaat atcgacgccg accacatggc gacctacggc ggcgacttca acaagctgaa gaagaccttc gtcgagttcc tccacaacct gccgttctac ggactggcgg tgatgtgcgt ggatgatccg gttgtgcgtg agatcctccc gcagatcgcc cgcccgaccg tgacctacgg cctcagcgaa gacgccgacg tgcgcgcgat caacatccgc caggaaggca tgcgcacctg gttcaccgtg ttgcgcccgg agcgcgagcc gctggacgtc tcggtgaaca tgcccggcct gcacaacgtg ctgaattccc tggcgaccat cgtcatcgct accgacgagg gcatctccga cgaagccatc gtccaggggc tgtccggctt ccagggcgta ggccggcgct tccaggtcta cggcgagctg caggtcgagg gtggcagcgt gatgctggtg gacgattacg gccaccatcc gcgcgaagtc gccgcggtga tcaaggcgat ccgtggcggt tggccggagc gtcgcctggt gatggtctac cagccgcatc gctatacccg tacccgcgac ctgtacgaag acttcgtgca ggtgctgggc gaagccaacg tgctgctgtt gatggaggtc tatccggccg gcgaagagcc gatcccggga gccgacagcc gccagctgtg ccacagcatc cgccagcgcg gccagcttga cccgatctac ttcgagcgcg acgccgacct ggcgccgctg gtcaagccgc tgctgcgcgc tggcgacatc ctgctttgcc agggcgctgg cgatgtcggc ggcctggccc cgcaactgat caagaacccg ctgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PsaeA.00137.b.B3.GE40417
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/22/2016 |
| EXPECTED MW: | 50kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMR RIRRIHFVGI GGAGMCGIAE VLLNLGYEVS GSDLKASAVT ERLEKFGAQI FIGHQAENAD GADVLVVSSA INRANPEVAS ALERRIPVVP RAEMLAELMR YRHGIAVAGT HGKTTTTSLI ASVFAAGGLD PTFVIGGRLN AAGTNAQLGA SRYLVAEADE SDASFLHLQP MVAVVTNIDA DHMATYGGDF NKLKKTFVEF LHNLPFYGLA VMCVDDPVVR EILPQIARPT VTYGLSEDAD VRAINIRQEG MRTWFTVLRP EREPLDVSVN MPGLHNVLNS LATIVIATDE GISDEAIVQG LSGFQGVGRR FQVYGELQVE GGSVMLVDDY GHHPREVAAV IKAIRGGWPE RRLVMVYQPH RYTRTRDLYE DFVQVLGEAN VLLLMEVYPA GEEPIPGADS RQLCHSIRQR GQLDPIYFER DADLAPLVKP LLRAGDILLC QGAGDVGGLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcc gtatccgccg catccatttc gtcggcatcg gcggcgccgg tatgtgcggg atcgccgaag tgctgctgaa cctcggctac gaggtatccg gctcggacct caaggcctcg gcggtgaccg agcgcctgga gaagttcggc gcgcagatct tcatcggcca ccaggcggaa aacgccgacg gcgccgacgt gctggtggtg tccagtgcca tcaaccgggc caacccggaa gtggcatcgg ccctggaacg gcggattccg gtggtgccgc gtgcggagat gctcgccgag ctgatgcgct accggcacgg catcgcggta gccggcaccc acggcaagac caccactacc agcctgatcg cctcggtgtt cgccgccggc ggcctggacc cgaccttcgt catcggcggc cggctgaacg ccgccgggac caacgcccag ctcggcgcca gccgctacct ggtggccgag gccgacgaga gcgacgccag cttcctgcac ctgcaaccga tggtcgcggt ggtcaccaat atcgacgccg accacatggc gacctacggc ggcgacttca acaagctgaa gaagaccttc gtcgagttcc tccacaacct gccgttctac ggactggcgg tgatgtgcgt ggatgatccg gttgtgcgtg agatcctccc gcagatcgcc cgcccgaccg tgacctacgg cctcagcgaa gacgccgacg tgcgcgcgat caacatccgc caggaaggca tgcgcacctg gttcaccgtg ttgcgcccgg agcgcgagcc gctggacgtc tcggtgaaca tgcccggcct gcacaacgtg ctgaattccc tggcgaccat cgtcatcgct accgacgagg gcatctccga cgaagccatc gtccaggggc tgtccggctt ccagggcgta ggccggcgct tccaggtcta cggcgagctg caggtcgagg gtggcagcgt gatgctggtg gacgattacg gccaccatcc gcgcgaagtc gccgcggtga tcaaggcgat ccgtggcggt tggccggagc gtcgcctggt gatggtctac cagccgcatc gctatacccg tacccgcgac ctgtacgaag acttcgtgca ggtgctgggc gaagccaacg tgctgctgtt gatggaggtc tatccggccg gcgaagagcc gatcccggga gccgacagcc gccagctgtg ccacagcatc cgccagcgcg gccagcttga cccgatctac ttcgagcgcg acgccgacct ggcgccgctg gtcaagccgc tgctgcgcgc tggcgacatc ctgctttgcc agggcgctgg cgatgtcggc ggcctggcct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for PsaeA.00137.b.B4.GE40418
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHRI HFVGIGGAGM CGIAEVLLNL GYEVSGSDLK ASAVTERLEK FGAQIFIGHQ AENADGADVL VVSSAINRAN PEVASALERR IPVVPRAEML AELMRYRHGI AVAGTHGKTT TTSLIASVFA AGGLDPTFVI GGRLNAAGTN AQLGASRYLV AEADESDASF LHLQPMVAVV TNIDADHMAT YGGDFNKLKK TFVEFLHNLP FYGLAVMCVD DPVVREILPQ IARPTVTYGL SEDADVRAIN IRQEGMRTWF TVLRPEREPL DVSVNMPGLH NVLNSLATIV IATDEGISDE AIVQGLSGFQ GVGRRFQVYG ELQVEGGSVM LVDDYGHHPR EVAAVIKAIR GGWPERRLVM VYQPHRYTRT RDLYED
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgcatcc atttcgtcgg catcggcggc gccggtatgt gcgggatcgc cgaagtgctg ctgaacctcg gctacgaggt atccggctcg gacctcaagg cctcggcggt gaccgagcgc ctggagaagt tcggcgcgca gatcttcatc ggccaccagg cggaaaacgc cgacggcgcc gacgtgctgg tggtgtccag tgccatcaac cgggccaacc cggaagtggc atcggccctg gaacggcgga ttccggtggt gccgcgtgcg gagatgctcg ccgagctgat gcgctaccgg cacggcatcg cggtagccgg cacccacggc aagaccacca ctaccagcct gatcgcctcg gtgttcgccg ccggcggcct ggacccgacc ttcgtcatcg gcggccggct gaacgccgcc gggaccaacg cccagctcgg cgccagccgc tacctggtgg ccgaggccga cgagagcgac gccagcttcc tgcacctgca accgatggtc gcggtggtca ccaatatcga cgccgaccac atggcgacct acggcggcga cttcaacaag ctgaagaaga ccttcgtcga gttcctccac aacctgccgt tctacggact ggcggtgatg tgcgtggatg atccggttgt gcgtgagatc ctcccgcaga tcgcccgccc gaccgtgacc tacggcctca gcgaagacgc cgacgtgcgc gcgatcaaca tccgccagga aggcatgcgc acctggttca ccgtgttgcg cccggagcgc gagccgctgg acgtctcggt gaacatgccc ggcctgcaca acgtgctgaa ttccctggcg accatcgtca tcgctaccga cgagggcatc tccgacgaag ccatcgtcca ggggctgtcc ggcttccagg gcgtaggccg gcgcttccag gtctacggcg agctgcaggt cgagggtggc agcgtgatgc tggtggacga ttacggccac catccgcgcg aagtcgccgc ggtgatcaag gcgatccgtg gcggttggcc ggagcgtcgc ctggtgatgg tctaccagcc gcatcgctat acccgtaccc gcgacctgta cgaagactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for PsaeA.00137.b.B5.GE40419
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 34kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHRR IHFVGIGGAG MCGIAEVLLN LGYEVSGSDL KASAVTERLE KFGAQIFIGH QAENADGADV LVVSSAINRA NPEVASALER RIPVVPRAEM LAELMRYRHG IAVAGTHGKT TTTSLIASVF AAGGLDPTFV IGGRLNAAGT NAQLGASRYL VAEADESDAS FLHLQPMVAV VTNIDADHMA TYGGDFNKLK KTFVEFLHNL PFYGLAVMCV DDPVVREILP QIARPTVTYG LSEDADVRAI NIRQEGMRTW FTVLRPEREP LDVSVNMPGL HNVLNSLATI VIATDEGISD EAIVQGLSGF QGVGR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgccgca tccatttcgt cggcatcggc ggcgccggta tgtgcgggat cgccgaagtg ctgctgaacc tcggctacga ggtatccggc tcggacctca aggcctcggc ggtgaccgag cgcctggaga agttcggcgc gcagatcttc atcggccacc aggcggaaaa cgccgacggc gccgacgtgc tggtggtgtc cagtgccatc aaccgggcca acccggaagt ggcatcggcc ctggaacggc ggattccggt ggtgccgcgt gcggagatgc tcgccgagct gatgcgctac cggcacggca tcgcggtagc cggcacccac ggcaagacca ccactaccag cctgatcgcc tcggtgttcg ccgccggcgg cctggacccg accttcgtca tcggcggccg gctgaacgcc gccgggacca acgcccagct cggcgccagc cgctacctgg tggccgaggc cgacgagagc gacgccagct tcctgcacct gcaaccgatg gtcgcggtgg tcaccaatat cgacgccgac cacatggcga cctacggcgg cgacttcaac aagctgaaga agaccttcgt cgagttcctc cacaacctgc cgttctacgg actggcggtg atgtgcgtgg atgatccggt tgtgcgtgag atcctcccgc agatcgcccg cccgaccgtg acctacggcc tcagcgaaga cgccgacgtg cgcgcgatca acatccgcca ggaaggcatg cgcacctggt tcaccgtgtt gcgcccggag cgcgagccgc tggacgtctc ggtgaacatg cccggcctgc acaacgtgct gaattccctg gcgaccatcg tcatcgctac cgacgagggc atctccgacg aagccatcgt ccaggggctg tccggcttcc agggcgtagg ccggtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for PsaeA.00137.b.B6.GE40420
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 49kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHGY EVSGSDLKAS AVTERLEKFG AQIFIGHQAE NADGADVLVV SSAINRANPE VASALERRIP VVPRAEMLAE LMRYRHGIAV AGTHGKTTTT SLIASVFAAG GLDPTFVIGG RLNAAGTNAQ LGASRYLVAE ADESDASFLH LQPMVAVVTN IDADHMATYG GDFNKLKKTF VEFLHNLPFY GLAVMCVDDP VVREILPQIA RPTVTYGLSE DADVRAINIR QEGMRTWFTV LRPEREPLDV SVNMPGLHNV LNSLATIVIA TDEGISDEAI VQGLSGFQGV GRRFQVYGEL QVEGGSVMLV DDYGHHPREV AAVIKAIRGG WPERRLVMVY QPHRYTRTRD LYEDFVQVLG EANVLLLMEV YPAGEEPIPG ADSRQLCHSI RQRGQLDPIY FERDADLAPL VKPLLRAGDI LLCQGAGDVG GLAPQLIKNP LFAGKGGKGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggctacg aggtatccgg ctcggacctc aaggcctcgg cggtgaccga gcgcctggag aagttcggcg cgcagatctt catcggccac caggcggaaa acgccgacgg cgccgacgtg ctggtggtgt ccagtgccat caaccgggcc aacccggaag tggcatcggc cctggaacgg cggattccgg tggtgccgcg tgcggagatg ctcgccgagc tgatgcgcta ccggcacggc atcgcggtag ccggcaccca cggcaagacc accactacca gcctgatcgc ctcggtgttc gccgccggcg gcctggaccc gaccttcgtc atcggcggcc ggctgaacgc cgccgggacc aacgcccagc tcggcgccag ccgctacctg gtggccgagg ccgacgagag cgacgccagc ttcctgcacc tgcaaccgat ggtcgcggtg gtcaccaata tcgacgccga ccacatggcg acctacggcg gcgacttcaa caagctgaag aagaccttcg tcgagttcct ccacaacctg ccgttctacg gactggcggt gatgtgcgtg gatgatccgg ttgtgcgtga gatcctcccg cagatcgccc gcccgaccgt gacctacggc ctcagcgaag acgccgacgt gcgcgcgatc aacatccgcc aggaaggcat gcgcacctgg ttcaccgtgt tgcgcccgga gcgcgagccg ctggacgtct cggtgaacat gcccggcctg cacaacgtgc tgaattccct ggcgaccatc gtcatcgcta ccgacgaggg catctccgac gaagccatcg tccaggggct gtccggcttc cagggcgtag gccggcgctt ccaggtctac ggcgagctgc aggtcgaggg tggcagcgtg atgctggtgg acgattacgg ccaccatccg cgcgaagtcg ccgcggtgat caaggcgatc cgtggcggtt ggccggagcg tcgcctggtg atggtctacc agccgcatcg ctatacccgt acccgcgacc tgtacgaaga cttcgtgcag gtgctgggcg aagccaacgt gctgctgttg atggaggtct atccggccgg cgaagagccg atcccgggag ccgacagccg ccagctgtgc cacagcatcc gccagcgcgg ccagcttgac ccgatctact tcgagcgcga cgccgacctg gcgccgctgg tcaagccgct gctgcgcgct ggcgacatcc tgctttgcca gggcgctggc gatgtcggcg gcctggcccc gcaactgatc aagaacccgc tgttcgccgg caagggaggg aagggcgcat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for PsaeA.00137.b.B1.PW38298
| PURIFICATION DATe: | 6/12/2017 |
| CONCENTRATION: | 33.2mg/ml |
| OBSERVED MW: | 56kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MRRIRRIHFV GIGGAGMCGI AEVLLNLGYE VSGSDLKASA VTERLEKFGA QIFIGHQAEN ADGADVLVVS SAINRANPEV ASALERRIPV VPRAEMLAEL MRYRHGIAVA GTHGKTTTTS LIASVFAAGG LDPTFVIGGR LNAAGTNAQL GASRYLVAEA DESDASFLHL QPMVAVVTNI DADHMATYGG DFNKLKKTFV EFLHNLPFYG LAVMCVDDPV VREILPQIAR PTVTYGLSED ADVRAINIRQ EGMRTWFTVL RPEREPLDVS VNMPGLHNVL NSLATIVIAT DEGISDEAIV QGLSGFQGVG RRFQVYGELQ VEGGSVMLVD DYGHHPREVA AVIKAIRGGW PERRLVMVYQ PHRYTRTRDL YEDFVQVLGE ANVLLLMEVY PAGEEPIPGA DSRQLCHSIR QRGQLDPIYF ERDADLAPLV KPLLRAGDIL LCQGAGDVGG LAPQLIKNPL FAGKGGKGA
Validated NT Sequence
atcttactca cttatgcgcc cttccctccc ttgccggcga acagcgggtt cttgatcagt tgcggggcca ggccgccgac atcgccagcg ccctggcaaa gcaggatgtc gccagcgcgc agcagcggct tgaccagcgg cgccaggtcg gcgtcgcgct cgaagtagat cgggtcaagc tggccgcgct ggcggatgct gtggcacagc tggcggctgt cggctcccgg gatcggctct tcgccggccg gatagacctc catcaacagc agcacgttgg cttcgcccag cacctgcacg aagtcttcgt acaggtcgcg ggtacgggta tagcgatgcg gctggtagac catcaccagg cgacgctccg gccaaccgcc acggatcgcc ttgatcaccg cggcgacttc gcgcggatgg tggccgtaat cgtccaccag catcacgctg ccaccctcga cctgcagctc gccgtagacc tggaagcgcc ggcctacgcc ctggaagccg gacagcccct ggacgatggc ttcgtcggag atgccctcgt cggtagcgat gacgatggtc gccagggaat tcagcacgtt gtgcaggccg ggcatgttca ccgagacgtc cagcggctcg cgctccgggc gcaacacggt gaaccaggtg cgcatgcctt cctggcggat gttgatcgcg cgcacgtcgg cgtcttcgct gaggccgtag gtcacggtcg ggcgggcgat ctgcgggagg atctcacgca caaccggatc atccacgcac atcaccgcca gtccgtagaa cggcaggttg tggaggaact cgacgaaggt cttcttcagc ttgttgaagt cgccgccgta ggtcgccatg tggtcggcgt cgatattggt gaccaccgcg accatcggtt gcaggtgcag gaagctggcg tcgctctcgt cggcctcggc caccaggtag cggctggcgc cgagctgggc gttggtcccg gcggcgttca gccggccgcc gatgacgaag gtcgggtcca ggccgccggc ggcgaacacc gaggcgatca ggctggtagt ggtggtcttg ccgtgggtgc cggctaccgc gatgccgtgc cggtagcgca tcagctcggc gagcatctcc gcacgcggca ccaccggaat ccgccgttcc agggccgatg ccacttccgg gttggcccgg ttgatggcac tggacaccac cagcacgtcg gcgccgtcgg cgttttccgc ctggtggccg atgaagatct gcgcgccgaa cttctccagg cgctcggtca ccgccgaggc cttgaggtcc gagccggata cctcgtagcc gaggttcagc agcacttcgg cgatcccgca cataccggcg ccgccgatgc cgacgaaatg gatgcggcgg atacggcgca tggtccgggt gacgccattc ggttcttta
Expressed Protein Sequence
MAHHHHHHMV KEPNGVTRTM RRIRRIHFVG IGGAGMCGIA EVLLNLGYEV SGSDLKASAV TERLEKFGAQ IFIGHQAENA DGADVLVVSS AINRANPEVA SALERRIPVV PRAEMLAELM RYRHGIAVAG THGKTTTTSL IASVFAAGGL DPTFVIGGRL NAAGTNAQLG ASRYLVAEAD ESDASFLHLQ PMVAVVTNID ADHMATYGGD FNKLKKTFVE FLHNLPFYGL AVMCVDDPVV REILPQIARP TVTYGLSEDA DVRAINIRQE GMRTWFTVLR PEREPLDVSV NMPGLHNVLN SLATIVIATD EGISDEAIVQ GLSGFQGVGR RFQVYGELQV EGGSVMLVDD YGHHPREVAA VIKAIRGGWP ERRLVMVYQP HRYTRTRDLY EDFVQVLGEA NVLLLMEVYP AGEEPIPGAD SRQLCHSIRQ RGQLDPIYFE RDADLAPLVK PLLRAGDILL CQGAGDVGGL APQLIKNPLF AGKGGKGA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGGTTA AAGAACCGAA TGGCGTCACC CGGACCATGC GCCGTATCCG CCGCATCCAT TTCGTCGGCA TCGGCGGCGC CGGTATGTGC GGGATCGCCG AAGTGCTGCT GAACCTCGGC TACGAGGTAT CCGGCTCGGA CCTCAAGGCC TCGGCGGTGA CCGAGCGCCT GGAGAAGTTC GGCGCGCAGA TCTTCATCGG CCACCAGGCG GAAAACGCCG ACGGCGCCGA CGTGCTGGTG GTGTCCAGTG CCATCAACCG GGCCAACCCG GAAGTGGCAT CGGCCCTGGA ACGGCGGATT CCGGTGGTGC CGCGTGCGGA GATGCTCGCC GAGCTGATGC GCTACCGGCA CGGCATCGCG GTAGCCGGCA CCCACGGCAA GACCACCACT ACCAGCCTGA TCGCCTCGGT GTTCGCCGCC GGCGGCCTGG ACCCGACCTT CGTCATCGGC GGCCGGCTGA ACGCCGCCGG GACCAACGCC CAGCTCGGCG CCAGCCGCTA CCTGGTGGCC GAGGCCGACG AGAGCGACGC CAGCTTCCTG CACCTGCAAC CGATGGTCGC GGTGGTCACC AATATCGACG CCGACCACAT GGCGACCTAC GGCGGCGACT TCAACAAGCT GAAGAAGACC TTCGTCGAGT TCCTCCACAA CCTGCCGTTC TACGGACTGG CGGTGATGTG CGTGGATGAT CCGGTTGTGC GTGAGATCCT CCCGCAGATC GCCCGCCCGA CCGTGACCTA CGGCCTCAGC GAAGACGCCG ACGTGCGCGC GATCAACATC CGCCAGGAAG GCATGCGCAC CTGGTTCACC GTGTTGCGCC CGGAGCGCGA GCCGCTGGAC GTCTCGGTGA ACATGCCCGG CCTGCACAAC GTGCTGAATT CCCTGGCGAC CATCGTCATC GCTACCGACG AGGGCATCTC CGACGAAGCC ATCGTCCAGG GGCTGTCCGG CTTCCAGGGC GTAGGCCGGC GCTTCCAGGT CTACGGCGAG CTGCAGGTCG AGGGTGGCAG CGTGATGCTG GTGGACGATT ACGGCCACCA TCCGCGCGAA GTCGCCGCGG TGATCAAGGC GATCCGTGGC GGTTGGCCGG AGCGTCGCCT GGTGATGGTC TACCAGCCGC ATCGCTATAC CCGTACCCGC GACCTGTACG AAGACTTCGT GCAGGTGCTG GGCGAAGCCA ACGTGCTGCT GTTGATGGAG GTCTATCCGG CCGGCGAAGA GCCGATCCCG GGAGCCGACA GCCGCCAGCT GTGCCACAGC ATCCGCCAGC GCGGCCAGCT TGACCCGATC TACTTCGAGC GCGACGCCGA CCTGGCGCCG CTGGTCAAGC CGCTGCTGCG CGCTGGCGAC ATCCTGCTTT GCCAGGGCGC TGGCGATGTC GGCGGCCTGG CCCCGCAACT GATCAAGAAC CCGCTGTTCG CCGGCAAGGG AGGGAAGGGC GCATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT