PsaeA.00137.a

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (EC 6.3.2.10) (MurF)

CENTER ID: PsaeA.00137.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00137.a.B1.GE38202 full length 1 458
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4625
PGAT: pseudomonas:PA4416
RefSeq: NP_253106.1
UniProt: Q9HVZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLEPLRLSQL TVALDARLIG EDAVFSAVST DSRAIGPGQL FIALSGPRFD GHDYLAEVAA KGAVAALVER EVADAPLPQL LVRDTRAALG RLGALNRRKF TGPLAAMTGS SGKTTVKEML ASILRTQAGD AESVLATRGN LNNDLGVPLT LLQLAPQHRS AVIELGASRI GEIAYTVELT RPHVAIITNA GTAHVGEFGG PEKIVEAKGE ILEGLAADGT AVLNLDDKAF DTWKARASGR PLLTFSLDRP QADFRAADLQ RDARGCMGFR LQGVAGEAQV QLNLLGRHNV ANALAAAAAA HALGVPLDGI VAGLQALQPV KGRAVAQLTA SGLRVIDDSY NANPASMLAA IDILSGFSGR TVLVLGDMGE LGSWAEQAHR EVGAYAAGKV SALYAVGPLM AHAVQAFGAT GRHFADQASL IGALATEQPT TTILIKGSRS AAMDKVVAAL CGSSEESH
NT Sequence
atgcttgagc ctcttcgcct cagccagttg acggtcgcgc tggacgcccg cctgatcggc gaggacgccg tcttttcggc ggtttccacc gacagtcgcg ccatcgggcc cggccaactg ttcattgccc tgagtgggcc gcgtttcgac ggccacgact atctcgccga ggttgccgcc aagggcgcgg tggctgcgct ggtggagcgc gaagtcgccg acgcgcccct gccgcaattg ctggtgcgcg atacccgtgc ggccctgggg cgactgggcg cgctgaaccg gcgcaagttc accggcccgc tggcggccat gacgggctcc agcggcaaga ccacggtcaa ggagatgctc gccagcatcc tgcgtaccca ggccggcgat gccgagtcgg tgctggctac ccgtggcaat ctgaacaacg acctcggcgt accgctgacc ctgctgcaac tggcgccgca gcaccgtagc gcagtgatcg aactgggcgc ctcgcgcatc ggcgagatcg cctacacggt cgagctgacc cgcccgcacg tggcgatcat caccaatgcc ggaaccgccc atgtcggcga gttcggcgga ccggagaaga tcgtcgaggc gaagggcgag atactcgaag ggctggccgc cgacggcacc gccgtactga acctggacga caaggccttc gacacctgga aggcccgtgc cagcggccgt ccgttgctga ctttctccct cgaccggccc caggccgatt tccgcgccgc cgatctgcag cgcgatgcgc gcggctgcat gggcttcagg ctgcagggcg tagcgggtga agcgcaggtc cagctcaacc tgctggggcg gcacaatgtc gccaatgccc tggctgccgc cgctgccgcc catgcactgg gcgtgccgct ggatgggatc gtcgccgggc tgcaggcgct gcagccggtc aagggccgcg cggtagcgca actgaccgcc agcgggctgc gtgtgataga cgacagctac aacgccaacc ccgcgtcaat gctggcggcg attgatatac tgagcggctt ttccgggcgc accgtcctgg tcctcggaga catgggcgaa ctcggttcct gggccgagca ggcccaccgc gaggtgggcg cctacgccgc tggcaaggtg tccgcgctct atgcggtcgg accgctgatg gcccacgccg tacaggcgtt cggcgccacg ggccggcact tcgccgacca ggccagcctg atcggggcgc tggccaccga acaaccgaca accaccattt tgatcaaggg ttcccgcagt gcggcgatgg acaaagtcgt cgcggcgctg tgcggttcct ccgaggagag tcactaa
Details for PsaeA.00137.a.B1.GE38202
HARVESTED ON: 6/11/2014
SEQUENCED ON: 6/12/2014
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 89
PERCENT COVERAGE: 71
Validated AA Sequence
MAHHHHHHML EPLRLSQLTV ALDARLIGED AVFSAVSTDS RAIGPGQLFI ALSGPRFDGH DYLAEVAAKG AVAALVEREV ADAPLPQLLV RDTRAALGRL GALNRRKFTG PLAAMTGSSG KTTVKEMLAS ILRTQAGDAE SVLATRGNLN NDLGVPLTLL QLAPQHRSAV IELGASRIGE IAYTVELTRP HVAIITNAGT AHVGEFGGPE KIVEAKGEIL EGLAADGTAV LNLDDKAFDT WKARASGRPL LTFSLDRPQA DFRAADLQRD ARGCMGFRLQ GVRVKRSPAQ PAGAAQCRQC PGCRRCRPCT GRAAGWXRRX LXRCSRSRXA R
Validated NT Sequence
cnctagaata ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatgctt gagcctcttc gcctcagcca gttgacggtc gcgctggacg cccgcctgat cggcgaggac gccgtctttt cggcggtttc caccgacagt cgcgccatcg ggcccggcca actgttcatt gccctgagtg ggccgcgttt cgacggccac gactatctcg ccgaggttgc cgccaagggc gcggtggctg cgctggtgga gcgcgaagtc gccgacgcgc ccctgccgca attgctggtg cgcgataccc gtgcggccct ggggcgactg ggcgcgctga accggcgcaa gttcaccggc ccgctggcgg ccatgacggg ctccagcggc aagaccacgg tcaaggagat gctcgccagc atcctgcgta cccaggccgg cgatgccgag tcggtgctgg ctacccgtgg caatctgaac aacgacctcg gcgtaccgct gaccctgctg caactggcgc cgcagcaccg tagcgcagtg atcgaactgg gcgcctcgcg catcggcgag atcgcctaca cggtcgagct gacccgcccg cacgtggcga tcatcaccaa tgccggaacc gcccatgtcg gcgagttcgg cggaccggag aagatcgtcg aggcgaaggg cgagatactc gaagggctgg ccgccgacgg caccgccgta ctgaacctgg acgacaaggc cttcgacacc tggaaggccc gtgccagcgg ccgtccgttg ctgactttct ccctcgaccg gccccaggcc gatttccgcg ccgccgatct gcagcgcgat gcgcgcggct gcatgggctt caggctgcag ggcgtacggg tgaagcgcag tccagctcaa cctgctgggg cggcacaatg tcgccaatgc cctggctgcc gccgctgccg cccatgcact gggcgtgccg ctggatgggn tcgtcgcnnn ctgcnncgct gcagccggtc aagggncgcg cggtagcgca actgaccgcc agcggctgcg tgtgatagac gacagctaca acnccaaccc cgcgtcaatg ctggcngnga tgannatact gnnnnnnttt nnnnggnnnc accgtnnngg nnnnnnnnca tgggnnaact nnnnnngncn ncnnnagnnn nnnntanncn ncnngncaan nnntncnnnc gnccct
Expected Protein Sequence
MAHHHHHHML EPLRLSQLTV ALDARLIGED AVFSAVSTDS RAIGPGQLFI ALSGPRFDGH DYLAEVAAKG AVAALVEREV ADAPLPQLLV RDTRAALGRL GALNRRKFTG PLAAMTGSSG KTTVKEMLAS ILRTQAGDAE SVLATRGNLN NDLGVPLTLL QLAPQHRSAV IELGASRIGE IAYTVELTRP HVAIITNAGT AHVGEFGGPE KIVEAKGEIL EGLAADGTAV LNLDDKAFDT WKARASGRPL LTFSLDRPQA DFRAADLQRD ARGCMGFRLQ GVAGEAQVQL NLLGRHNVAN ALAAAAAAHA LGVPLDGIVA GLQALQPVKG RAVAQLTASG LRVIDDSYNA NPASMLAAID ILSGFSGRTV LVLGDMGELG SWAEQAHREV GAYAAGKVSA LYAVGPLMAH AVQAFGATGR HFADQASLIG ALATEQPTTT ILIKGSRSAA MDKVVAALCG SSEESH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcttg agcctcttcg cctcagccag ttgacggtcg cgctggacgc ccgcctgatc ggcgaggacg ccgtcttttc ggcggtttcc accgacagtc gcgccatcgg gcccggccaa ctgttcattg ccctgagtgg gccgcgtttc gacggccacg actatctcgc cgaggttgcc gccaagggcg cggtggctgc gctggtggag cgcgaagtcg ccgacgcgcc cctgccgcaa ttgctggtgc gcgatacccg tgcggccctg gggcgactgg gcgcgctgaa ccggcgcaag ttcaccggcc cgctggcggc catgacgggc tccagcggca agaccacggt caaggagatg ctcgccagca tcctgcgtac ccaggccggc gatgccgagt cggtgctggc tacccgtggc aatctgaaca acgacctcgg cgtaccgctg accctgctgc aactggcgcc gcagcaccgt agcgcagtga tcgaactggg cgcctcgcgc atcggcgaga tcgcctacac ggtcgagctg acccgcccgc acgtggcgat catcaccaat gccggaaccg cccatgtcgg cgagttcggc ggaccggaga agatcgtcga ggcgaagggc gagatactcg aagggctggc cgccgacggc accgccgtac tgaacctgga cgacaaggcc ttcgacacct ggaaggcccg tgccagcggc cgtccgttgc tgactttctc cctcgaccgg ccccaggccg atttccgcgc cgccgatctg cagcgcgatg cgcgcggctg catgggcttc aggctgcagg gcgtagcggg tgaagcgcag gtccagctca acctgctggg gcggcacaat gtcgccaatg ccctggctgc cgccgctgcc gcccatgcac tgggcgtgcc gctggatggg atcgtcgccg ggctgcaggc gctgcagccg gtcaagggcc gcgcggtagc gcaactgacc gccagcgggc tgcgtgtgat agacgacagc tacaacgcca accccgcgtc aatgctggcg gcgattgata tactgagcgg cttttccggg cgcaccgtcc tggtcctcgg agacatgggc gaactcggtt cctgggccga gcaggcccac cgcgaggtgg gcgcctacgc cgctggcaag gtgtccgcgc tctatgcggt cggaccgctg atggcccacg ccgtacaggc gttcggcgcc acgggccggc acttcgccga ccaggccagc ctgatcgggg cgctggccac cgaacaaccg acaaccacca ttttgatcaa gggttcccgc agtgcggcga tggacaaagt cgtcgcggcg ctgtgcggtt cctccgagga gagtcactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at