PsaeA.00133.a

Cysteine--tRNA ligase (EC 6.1.1.16) (Cysteinyl-tRNA synthetase) (CysRS)

CENTER ID: PsaeA.00133.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00133.a.B1.GE38201 full length 1 460
PsaeA.00133.a.B2.GE40414 truncation 1 1 452
PsaeA.00133.a.B3.GE40415 truncation 2 1 455
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00133.a.B3.PS38097 truncation 2 1 455
PsaeA.00133.a.B1.PW37531 full length 1 460
PsaeA.00133.a.B2.PW37945 truncation 1 1 452

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.1862
PGAT: pseudomonas:PA1795
RefSeq: NP_250486.1
UniProt: Q9I2U7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLQIYNTLSK TKEVFTPLVG NQVRMYVCGM TVYDYCHLGH GRSMVAFDVI TRWLRHRGYD LTYVRNITDI DDKIINRANE NGEPFDVLTE RMIAAMHEDE ARLNILKPDQ EPRATDHIAG MHAMIQTLID KGYAYAPGNG DVYYRVGKFA GYGKLSRRRV EDLRIGARIE PGEAKEDPLD FVLWKGAKPG EPSWSSPWGE GRPGWHIECS VMSTCCLGDS FDIHGGGNDL EFPHHENEIA QSEAATGKPY AKSWLHCGMI TINGEKMSKS LGNFFTIREV LEKYHPEVVR YLLIASHYRS PINYSEENLR EAKAALDRFY NALKGLPEAA PAEAAEYVER FAAAMDDDFN TAGACSVLFE LAREVNRLRE SDLSAAAALA ARLKQLAGLL GVLQLEPEAF LQAGAEGKVD AAEVEALIQA RLEARAAKNW AESDRIRDQL TAMGVVLEDG KGGTTWRLAD
NT Sequence
gtgctgcaga tctacaacac gctgagcaag accaaagaag tcttcacgcc gctggtcggc aaccaggtgc gcatgtacgt gtgcggcatg accgtgtacg actactgcca tctgggccac ggccgcagca tggtggcgtt cgacgtgatc acccgctggt tgcgccaccg tggctacgac ctgacgtacg tgcgcaacat caccgatatc gatgacaaga tcatcaatcg cgccaacgag aacggcgaac cgttcgatgt gctgaccgag cgcatgatcg cggcgatgca cgaggacgag gcccggctga acatcctcaa gccggaccag gagccgcgtg ccaccgatca catcgccggc atgcacgcga tgatccagac cctgatcgac aagggctatg cctacgcgcc gggcaacggt gacgtgtact accgggtcgg caagttcgct ggctacggca agttgtcgcg gcggcgggtc gaggacctgc gcatcggtgc gcggatcgaa ccgggcgaag cgaaggagga tccgctcgat ttcgtgctct ggaagggcgc caagccgggc gagccgagct ggtcgtcgcc ctggggcgag gggcgcccgg gctggcacat cgaatgctcg gtgatgtcca cctgctgcct gggcgattcc ttcgacatcc atggcggtgg caacgacctg gagttccctc accacgagaa cgagatcgcc cagagcgagg ccgcgaccgg caagccctac gccaagtcct ggctgcactg cgggatgatc accatcaacg gcgagaagat gtccaagtcg ctgggcaatt tcttcaccat ccgcgaagtg ctggagaaat atcaccccga agtggtgcgt tacctgctga tcgccagcca ttaccgcagt ccgatcaact actcggagga gaacctgcgc gaggccaagg cggccctgga ccgtttctac aacgcgctca aggggctgcc cgaggcggcg ccggcggaag cggcggagta tgtcgagcgc ttcgccgcgg cgatggacga cgacttcaat accgcgggtg cctgctcggt gctgttcgag ctggcgcggg aggtcaatcg tctgcgcgaa agcgacctgt cggcggcggc cgcgctggcc gcgcgcctga agcagttggc tggcctgctg ggggtgctgc aactggagcc ggaagccttc ctgcaggcgg gcgccgaggg caaggtcgat gcggccgagg tcgaggcgct gatccaggcg cgcctggagg cgcgggcggc gaagaactgg gcggagtccg accggattcg cgatcagctc accgccatgg gcgtagtgct ggaggatggc aagggcggca ctacctggcg cctggccgac tga
Details for PsaeA.00133.a.B1.GE38201
HARVESTED ON: 6/11/2014
SEQUENCED ON: 6/13/2014
EXPECTED MW: 52kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Validated AA Sequence
MVLQIYNTLS KTKEVFTPLV GNQVRMYVCG MTVYDYCHLG HGRSMVAFDV ITRWLRHRGY DLTYVRNITD IDDKIINRAN ENGEPFDVLT ERMIAAMHED EARLNILKPD QEPRATDHIA GMHAMIQTLI DKGYAYAPGN GDVYYRVGKF AGYGKLSRRR VEDLRIGARI EPGEAKEDPL DFVLWKGAKP GEPSWSSPWG EGRPGWHIEC SVMSTCCLGD SFDIHGGGND LEFPHHENEI AQSEAATGKP YAKSWLHCGM ITINGEKMSK SLGNFFTIRE VLEKYHPEVV RYLLIASHYR SPINYSEENL REAKAALDRF YNALKGLPEA APAEAAEYVE RFAAAMDDDF NTAGACSVLF ELAREVNRLR ESDLSAAAAL AARLKQLAGL LGVLQLEPEA FLQAGAEGKV DAAEVEALIQ ARLEARAAKN WAESDRIRDQ LTAMGVVLED GK
Validated NT Sequence
tcaccaccac caccaccata tggtgctgca gatctacaac acgctgagca agaccaaaga agtcttcacg ccgctggtcg gcaaccaggt gcgcatgtac gtgtgcggca tgaccgtgta cgactactgc catctgggcc acggccgcag catggtggcg ttcgacgtga tcacccgctg gttgcgccac cgtggctacg acctgacgta cgtgcgcaac atcaccgata tcgatgacaa gatcatcaat cgcgccaacg agaacggcga accgttcgat gtgctgaccg agcgcatgat cgcggcgatg cacgaggacg aggcccggct gaacatcctc aagccggacc aggagccgcg tgccaccgat cacatcgccg gcatgcacgc gatgatccag accctgatcg acaagggcta tgcctacgcg ccgggcaacg gtgacgtgta ctaccgggtc ggcaagttcg ctggctacgg caagttgtcg cggcggcggg tcgaggacct gcgcatcggt gcgcggatcg aaccgggcga agcgaaggag gatccgctcg atttcgtgct ctggaagggc gccaagccgg gcgagccgag ctggtcgtcg ccctggggcg aggggcgccc gggctggcac atcgaatgct cggtgatgtc cacctgctgc ctgggcgatt ccttcgacat ccatggcggt ggcaacgacc tggagttccc tcaccacgag aacgagatcg cccagagcga ggccgcgacc ggcaagccct acgccaagtc ctggctgcac tgcgggatga tcaccatcaa cggcgagaag atgtccaagt cgctgggcaa tttcttcacc atccgcgaag tgctggagaa atatcacccc gaagtggtgc gttacctgct gatcgccagc cattaccgca gtccgatcaa ctactcggag gagaacctgc gcgaggccaa ggcggccctg gaccgtttct acaacgcgct caaggggctg cccgaggcgg cgccggcgga agcggcggag tatgtcgagc gcttcgccgc ggcgatggac gacgacttca ataccgcggg tgcctgctcg gtgctgttcg agctggcgcg ggaggtcaat cgtctgcgcg aaagcgacct gtcggcggcg gccgcgctgg ccgcgcgcct gaagcagttg gctggcctgc tgggggtgct gcaactggag ccggaagcct tcctgcaggc gggcgccgag ggcaaggtcg atgcggccga ggtcgaggcg ctgatccagg cgcgcctgga ggcgcgggcg gcgaagaact gggcggagtc cgaccggatt cgcgatcagc tcaccgccat gggcgtagtg ctggaggatg gcaagggc
Expected Protein Sequence
MAHHHHHHML QIYNTLSKTK EVFTPLVGNQ VRMYVCGMTV YDYCHLGHGR SMVAFDVITR WLRHRGYDLT YVRNITDIDD KIINRANENG EPFDVLTERM IAAMHEDEAR LNILKPDQEP RATDHIAGMH AMIQTLIDKG YAYAPGNGDV YYRVGKFAGY GKLSRRRVED LRIGARIEPG EAKEDPLDFV LWKGAKPGEP SWSSPWGEGR PGWHIECSVM STCCLGDSFD IHGGGNDLEF PHHENEIAQS EAATGKPYAK SWLHCGMITI NGEKMSKSLG NFFTIREVLE KYHPEVVRYL LIASHYRSPI NYSEENLREA KAALDRFYNA LKGLPEAAPA EAAEYVERFA AAMDDDFNTA GACSVLFELA REVNRLRESD LSAAAALAAR LKQLAGLLGV LQLEPEAFLQ AGAEGKVDAA EVEALIQARL EARAAKNWAE SDRIRDQLTA MGVVLEDGKG GTTWRLAD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgctgc agatctacaa cacgctgagc aagaccaaag aagtcttcac gccgctggtc ggcaaccagg tgcgcatgta cgtgtgcggc atgaccgtgt acgactactg ccatctgggc cacggccgca gcatggtggc gttcgacgtg atcacccgct ggttgcgcca ccgtggctac gacctgacgt acgtgcgcaa catcaccgat atcgatgaca agatcatcaa tcgcgccaac gagaacggcg aaccgttcga tgtgctgacc gagcgcatga tcgcggcgat gcacgaggac gaggcccggc tgaacatcct caagccggac caggagccgc gtgccaccga tcacatcgcc ggcatgcacg cgatgatcca gaccctgatc gacaagggct atgcctacgc gccgggcaac ggtgacgtgt actaccgggt cggcaagttc gctggctacg gcaagttgtc gcggcggcgg gtcgaggacc tgcgcatcgg tgcgcggatc gaaccgggcg aagcgaagga ggatccgctc gatttcgtgc tctggaaggg cgccaagccg ggcgagccga gctggtcgtc gccctggggc gaggggcgcc cgggctggca catcgaatgc tcggtgatgt ccacctgctg cctgggcgat tccttcgaca tccatggcgg tggcaacgac ctggagttcc ctcaccacga gaacgagatc gcccagagcg aggccgcgac cggcaagccc tacgccaagt cctggctgca ctgcgggatg atcaccatca acggcgagaa gatgtccaag tcgctgggca atttcttcac catccgcgaa gtgctggaga aatatcaccc cgaagtggtg cgttacctgc tgatcgccag ccattaccgc agtccgatca actactcgga ggagaacctg cgcgaggcca aggcggccct ggaccgtttc tacaacgcgc tcaaggggct gcccgaggcg gcgccggcgg aagcggcgga gtatgtcgag cgcttcgccg cggcgatgga cgacgacttc aataccgcgg gtgcctgctc ggtgctgttc gagctggcgc gggaggtcaa tcgtctgcgc gaaagcgacc tgtcggcggc ggccgcgctg gccgcgcgcc tgaagcagtt ggctggcctg ctgggggtgc tgcaactgga gccggaagcc ttcctgcagg cgggcgccga gggcaaggtc gatgcggccg aggtcgaggc gctgatccag gcgcgcctgg aggcgcgggc ggcgaagaac tgggcggagt ccgaccggat tcgcgatcag ctcaccgcca tgggcgtagt gctggaggat ggcaagggcg gcactacctg gcgcctggcc gactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PsaeA.00133.a.B2.GE40414
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML QIYNTLSKTK EVFTPLVGNQ VRMYVCGMTV YDYCHLGHGR SMVAFDVITR WLRHRGYDLT YVRNITDIDD KIINRANENG EPFDVLTERM IAAMHEDEAR LNILKPDQEP RATDHIAGMH AMIQTLIDKG YAYAPGNGDV YYRVGKFAGY GKLSRRRVED LRIGARIEPG EAKEDPLDFV LWKGAKPGEP SWSSPWGEGR PGWHIECSVM STCCLGDSFD IHGGGNDLEF PHHENEIAQS EAATGKPYAK SWLHCGMITI NGEKMSKSLG NFFTIREVLE KYHPEVVRYL LIASHYRSPI NYSEENLREA KAALDRFYNA LKGLPEAAPA EAAEYVERFA AAMDDDFNTA GACSVLFELA REVNRLRESD LSAAAALAAR LKQLAGLLGV LQLEPEAFLQ AGAEGKVDAA EVEALIQARL EARAAKNWAE SDRIRDQLTA MGVVLEDGKG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgctgc agatctacaa cacgctgagc aagaccaaag aagtcttcac gccgctggtc ggcaaccagg tgcgcatgta cgtgtgcggc atgaccgtgt acgactactg ccatctgggc cacggccgca gcatggtggc gttcgacgtg atcacccgct ggttgcgcca ccgtggctac gacctgacgt acgtgcgcaa catcaccgat atcgatgaca agatcatcaa tcgcgccaac gagaacggcg aaccgttcga tgtgctgacc gagcgcatga tcgcggcgat gcacgaggac gaggcccggc tgaacatcct caagccggac caggagccgc gtgccaccga tcacatcgcc ggcatgcacg cgatgatcca gaccctgatc gacaagggct atgcctacgc gccgggcaac ggtgacgtgt actaccgggt cggcaagttc gctggctacg gcaagttgtc gcggcggcgg gtcgaggacc tgcgcatcgg tgcgcggatc gaaccgggcg aagcgaagga ggatccgctc gatttcgtgc tctggaaggg cgccaagccg ggcgagccga gctggtcgtc gccctggggc gaggggcgcc cgggctggca catcgaatgc tcggtgatgt ccacctgctg cctgggcgat tccttcgaca tccatggcgg tggcaacgac ctggagttcc ctcaccacga gaacgagatc gcccagagcg aggccgcgac cggcaagccc tacgccaagt cctggctgca ctgcgggatg atcaccatca acggcgagaa gatgtccaag tcgctgggca atttcttcac catccgcgaa gtgctggaga aatatcaccc cgaagtggtg cgttacctgc tgatcgccag ccattaccgc agtccgatca actactcgga ggagaacctg cgcgaggcca aggcggccct ggaccgtttc tacaacgcgc tcaaggggct gcccgaggcg gcgccggcgg aagcggcgga gtatgtcgag cgcttcgccg cggcgatgga cgacgacttc aataccgcgg gtgcctgctc ggtgctgttc gagctggcgc gggaggtcaa tcgtctgcgc gaaagcgacc tgtcggcggc ggccgcgctg gccgcgcgcc tgaagcagtt ggctggcctg ctgggggtgc tgcaactgga gccggaagcc ttcctgcagg cgggcgccga gggcaaggtc gatgcggccg aggtcgaggc gctgatccag gcgcgcctgg aggcgcgggc ggcgaagaac tgggcggagt ccgaccggat tcgcgatcag ctcaccgcca tgggcgtagt gctggaggat ggcaagggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for PsaeA.00133.a.B3.GE40415
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 52kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV LQIYNTLSKT KEVFTPLVGN QVRMYVCGMT VYDYCHLGHG RSMVAFDVIT RWLRHRGYDL TYVRNITDID DKIINRANEN GEPFDVLTER MIAAMHEDEA RLNILKPDQE PRATDHIAGM HAMIQTLIDK GYAYAPGNGD VYYRVGKFAG YGKLSRRRVE DLRIGARIEP GEAKEDPLDF VLWKGAKPGE PSWSSPWGEG RPGWHIECSV MSTCCLGDSF DIHGGGNDLE FPHHENEIAQ SEAATGKPYA KSWLHCGMIT INGEKMSKSL GNFFTIREVL EKYHPEVVRY LLIASHYRSP INYSEENLRE AKAALDRFYN ALKGLPEAAP AEAAEYVERF AAAMDDDFNT AGACSVLFEL AREVNRLRES DLSAAAALAA RLKQLAGLLG VLQLEPEAFL QAGAEGKVDA AEVEALIQAR LEARAAKNWA ESDRIRDQLT AMGVVL
Validated NT Sequence
ctccagcact acgcccatgg cggtgagctg atcgcgaatc cggtcggact ccgcccagtt cttcgccgcc cgcgcctcca ggcgcgcctg gatcagcgcc tcgacctcgg ccgcatcgac cttgccctcg gcgcccgcct gcaggaaggc ttccggctcc agttgcagca cccccagcag gccagccaac tgcttcaggc gcgcggccag cgcggccgcc gccgacaggt cgctttcgcg cagacgattg acctcccgcg ccagctcgaa cagcaccgag caggcacccg cggtattgaa gtcgtcgtcc atcgccgcgg cgaagcgctc gacatactcc gccgcttccg ccggcgccgc ctcgggcagc cccttgagcg cgttgtagaa acggtccagg gccgccttgg cctcgcgcag gttctcctcc gagtagttga tcggactgcg gtaatggctg gcgatcagca ggtaacgcac cacttcgggg tgatatttct ccagcacttc gcggatggtg aagaaattgc ccagcgactt ggacatcttc tcgccgttga tggtgatcat cccgcagtgc agccaggact tggcgtaggg cttgccggtc gcggcctcgc tctgggcgat ctcgttctcg tggtgaggga actccaggtc gttgccaccg ccatggatgt cgaaggaatc gcccaggcag caggtggaca tcaccgagca ttcgatgtgc cagcccgggc gcccctcgcc ccagggcgac gaccagctcg gctcgcccgg cttggcgccc ttccagagca cgaaatcgag cggatcctcc ttcgcttcgc ccggttcgat ccgcgcaccg atgcgcaggt cctcgacccg ccgccgcgac aacttgccgt agccagcgaa cttgccgacc cggtagtaca cgtcaccgtt gcccggcgcg taggcatagc ccttgtcgat cagggtctgg atcatcgcgt gcatgccggc gatgtgatcg gtggcacgcg gctcctggtc cggcttgagg atgttcagcc gggcctcgtc ctcgtgcatc gccgcgatca tgcgctcggt cagcacatcg aacggttcgc cgttctcgtt ggcgcgattg atgatcttgt catcgatatc ggtgatgttg cgcacgtacg tcaggtcgta gccacggtgg cgcaaccagc gggtgatcac gtcgaacgcc accatgctgc ggccgtggcc cagatggcag tagtcgtaca cggtcatgcc gcacacgtac atgcgcacct ggttgccgac cagcggcgtg aagacttctt tggtcttgct cagcgtgttg tagatctgca gcaccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHML QIYNTLSKTK EVFTPLVGNQ VRMYVCGMTV YDYCHLGHGR SMVAFDVITR WLRHRGYDLT YVRNITDIDD KIINRANENG EPFDVLTERM IAAMHEDEAR LNILKPDQEP RATDHIAGMH AMIQTLIDKG YAYAPGNGDV YYRVGKFAGY GKLSRRRVED LRIGARIEPG EAKEDPLDFV LWKGAKPGEP SWSSPWGEGR PGWHIECSVM STCCLGDSFD IHGGGNDLEF PHHENEIAQS EAATGKPYAK SWLHCGMITI NGEKMSKSLG NFFTIREVLE KYHPEVVRYL LIASHYRSPI NYSEENLREA KAALDRFYNA LKGLPEAAPA EAAEYVERFA AAMDDDFNTA GACSVLFELA REVNRLRESD LSAAAALAAR LKQLAGLLGV LQLEPEAFLQ AGAEGKVDAA EVEALIQARL EARAAKNWAE SDRIRDQLTA MGVVLEDGKG GTT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgctgc agatctacaa cacgctgagc aagaccaaag aagtcttcac gccgctggtc ggcaaccagg tgcgcatgta cgtgtgcggc atgaccgtgt acgactactg ccatctgggc cacggccgca gcatggtggc gttcgacgtg atcacccgct ggttgcgcca ccgtggctac gacctgacgt acgtgcgcaa catcaccgat atcgatgaca agatcatcaa tcgcgccaac gagaacggcg aaccgttcga tgtgctgacc gagcgcatga tcgcggcgat gcacgaggac gaggcccggc tgaacatcct caagccggac caggagccgc gtgccaccga tcacatcgcc ggcatgcacg cgatgatcca gaccctgatc gacaagggct atgcctacgc gccgggcaac ggtgacgtgt actaccgggt cggcaagttc gctggctacg gcaagttgtc gcggcggcgg gtcgaggacc tgcgcatcgg tgcgcggatc gaaccgggcg aagcgaagga ggatccgctc gatttcgtgc tctggaaggg cgccaagccg ggcgagccga gctggtcgtc gccctggggc gaggggcgcc cgggctggca catcgaatgc tcggtgatgt ccacctgctg cctgggcgat tccttcgaca tccatggcgg tggcaacgac ctggagttcc ctcaccacga gaacgagatc gcccagagcg aggccgcgac cggcaagccc tacgccaagt cctggctgca ctgcgggatg atcaccatca acggcgagaa gatgtccaag tcgctgggca atttcttcac catccgcgaa gtgctggaga aatatcaccc cgaagtggtg cgttacctgc tgatcgccag ccattaccgc agtccgatca actactcgga ggagaacctg cgcgaggcca aggcggccct ggaccgtttc tacaacgcgc tcaaggggct gcccgaggcg gcgccggcgg aagcggcgga gtatgtcgag cgcttcgccg cggcgatgga cgacgacttc aataccgcgg gtgcctgctc ggtgctgttc gagctggcgc gggaggtcaa tcgtctgcgc gaaagcgacc tgtcggcggc ggccgcgctg gccgcgcgcc tgaagcagtt ggctggcctg ctgggggtgc tgcaactgga gccggaagcc ttcctgcagg cgggcgccga gggcaaggtc gatgcggccg aggtcgaggc gctgatccag gcgcgcctgg aggcgcgggc ggcgaagaac tgggcggagt ccgaccggat tcgcgatcag ctcaccgcca tgggcgtagt gctggaggat ggcaagggcg gcactacctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for PsaeA.00133.a.B3.PS38097
PURIFICATION DATe: 11/3/2016
CONCENTRATION: 27.3mg/ml
OBSERVED MW: 50kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV LQIYNTLSKT KEVFTPLVGN QVRMYVCGMT VYDYCHLGHG RSMVAFDVIT RWLRHRGYDL TYVRNITDID DKIINRANEN GEPFDVLTER MIAAMHEDEA RLNILKPDQE PRATDHIAGM HAMIQTLIDK GYAYAPGNGD VYYRVGKFAG YGKLSRRRVE DLRIGARIEP GEAKEDPLDF VLWKGAKPGE PSWSSPWGEG RPGWHIECSV MSTCCLGDSF DIHGGGNDLE FPHHENEIAQ SEAATGKPYA KSWLHCGMIT INGEKMSKSL GNFFTIREVL EKYHPEVVRY LLIASHYRSP INYSEENLRE AKAALDRFYN ALKGLPEAAP AEAAEYVERF AAAMDDDFNT AGACSVLFEL AREVNRLRES DLSAAAALAA RLKQLAGLLG VLQLEPEAFL QAGAEGKVDA AEVEALIQAR LEARAAKNWA ESDRIRDQLT AMGVVL
Validated NT Sequence
ctccagcact acgcccatgg cggtgagctg atcgcgaatc cggtcggact ccgcccagtt cttcgccgcc cgcgcctcca ggcgcgcctg gatcagcgcc tcgacctcgg ccgcatcgac cttgccctcg gcgcccgcct gcaggaaggc ttccggctcc agttgcagca cccccagcag gccagccaac tgcttcaggc gcgcggccag cgcggccgcc gccgacaggt cgctttcgcg cagacgattg acctcccgcg ccagctcgaa cagcaccgag caggcacccg cggtattgaa gtcgtcgtcc atcgccgcgg cgaagcgctc gacatactcc gccgcttccg ccggcgccgc ctcgggcagc cccttgagcg cgttgtagaa acggtccagg gccgccttgg cctcgcgcag gttctcctcc gagtagttga tcggactgcg gtaatggctg gcgatcagca ggtaacgcac cacttcgggg tgatatttct ccagcacttc gcggatggtg aagaaattgc ccagcgactt ggacatcttc tcgccgttga tggtgatcat cccgcagtgc agccaggact tggcgtaggg cttgccggtc gcggcctcgc tctgggcgat ctcgttctcg tggtgaggga actccaggtc gttgccaccg ccatggatgt cgaaggaatc gcccaggcag caggtggaca tcaccgagca ttcgatgtgc cagcccgggc gcccctcgcc ccagggcgac gaccagctcg gctcgcccgg cttggcgccc ttccagagca cgaaatcgag cggatcctcc ttcgcttcgc ccggttcgat ccgcgcaccg atgcgcaggt cctcgacccg ccgccgcgac aacttgccgt agccagcgaa cttgccgacc cggtagtaca cgtcaccgtt gcccggcgcg taggcatagc ccttgtcgat cagggtctgg atcatcgcgt gcatgccggc gatgtgatcg gtggcacgcg gctcctggtc cggcttgagg atgttcagcc gggcctcgtc ctcgtgcatc gccgcgatca tgcgctcggt cagcacatcg aacggttcgc cgttctcgtt ggcgcgattg atgatcttgt catcgatatc ggtgatgttg cgcacgtacg tcaggtcgta gccacggtgg cgcaaccagc gggtgatcac gtcgaacgcc accatgctgc ggccgtggcc cagatggcag tagtcgtaca cggtcatgcc gcacacgtac atgcgcacct ggttgccgac cagcggcgtg aagacttctt tggtcttgct cagcgtgttg tagatctgca gcaccatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHML QIYNTLSKTK EVFTPLVGNQ VRMYVCGMTV YDYCHLGHGR SMVAFDVITR WLRHRGYDLT YVRNITDIDD KIINRANENG EPFDVLTERM IAAMHEDEAR LNILKPDQEP RATDHIAGMH AMIQTLIDKG YAYAPGNGDV YYRVGKFAGY GKLSRRRVED LRIGARIEPG EAKEDPLDFV LWKGAKPGEP SWSSPWGEGR PGWHIECSVM STCCLGDSFD IHGGGNDLEF PHHENEIAQS EAATGKPYAK SWLHCGMITI NGEKMSKSLG NFFTIREVLE KYHPEVVRYL LIASHYRSPI NYSEENLREA KAALDRFYNA LKGLPEAAPA EAAEYVERFA AAMDDDFNTA GACSVLFELA REVNRLRESD LSAAAALAAR LKQLAGLLGV LQLEPEAFLQ AGAEGKVDAA EVEALIQARL EARAAKNWAE SDRIRDQLTA MGVVLEDGKG GTT
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGCTGC AGATCTACAA CACGCTGAGC AAGACCAAAG AAGTCTTCAC GCCGCTGGTC GGCAACCAGG TGCGCATGTA CGTGTGCGGC ATGACCGTGT ACGACTACTG CCATCTGGGC CACGGCCGCA GCATGGTGGC GTTCGACGTG ATCACCCGCT GGTTGCGCCA CCGTGGCTAC GACCTGACGT ACGTGCGCAA CATCACCGAT ATCGATGACA AGATCATCAA TCGCGCCAAC GAGAACGGCG AACCGTTCGA TGTGCTGACC GAGCGCATGA TCGCGGCGAT GCACGAGGAC GAGGCCCGGC TGAACATCCT CAAGCCGGAC CAGGAGCCGC GTGCCACCGA TCACATCGCC GGCATGCACG CGATGATCCA GACCCTGATC GACAAGGGCT ATGCCTACGC GCCGGGCAAC GGTGACGTGT ACTACCGGGT CGGCAAGTTC GCTGGCTACG GCAAGTTGTC GCGGCGGCGG GTCGAGGACC TGCGCATCGG TGCGCGGATC GAACCGGGCG AAGCGAAGGA GGATCCGCTC GATTTCGTGC TCTGGAAGGG CGCCAAGCCG GGCGAGCCGA GCTGGTCGTC GCCCTGGGGC GAGGGGCGCC CGGGCTGGCA CATCGAATGC TCGGTGATGT CCACCTGCTG CCTGGGCGAT TCCTTCGACA TCCATGGCGG TGGCAACGAC CTGGAGTTCC CTCACCACGA GAACGAGATC GCCCAGAGCG AGGCCGCGAC CGGCAAGCCC TACGCCAAGT CCTGGCTGCA CTGCGGGATG ATCACCATCA ACGGCGAGAA GATGTCCAAG TCGCTGGGCA ATTTCTTCAC CATCCGCGAA GTGCTGGAGA AATATCACCC CGAAGTGGTG CGTTACCTGC TGATCGCCAG CCATTACCGC AGTCCGATCA ACTACTCGGA GGAGAACCTG CGCGAGGCCA AGGCGGCCCT GGACCGTTTC TACAACGCGC TCAAGGGGCT GCCCGAGGCG GCGCCGGCGG AAGCGGCGGA GTATGTCGAG CGCTTCGCCG CGGCGATGGA CGACGACTTC AATACCGCGG GTGCCTGCTC GGTGCTGTTC GAGCTGGCGC GGGAGGTCAA TCGTCTGCGC GAAAGCGACC TGTCGGCGGC GGCCGCGCTG GCCGCGCGCC TGAAGCAGTT GGCTGGCCTG CTGGGGGTGC TGCAACTGGA GCCGGAAGCC TTCCTGCAGG CGGGCGCCGA GGGCAAGGTC GATGCGGCCG AGGTCGAGGC GCTGATCCAG GCGCGCCTGG AGGCGCGGGC GGCGAAGAAC TGGGCGGAGT CCGACCGGAT TCGCGATCAG CTCACCGCCA TGGGCGTAGT GCTGGAGGAT GGCAAGGGCG GCACTACCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT
Details for PsaeA.00133.a.B1.PW37531
PURIFICATION DATe: 7/28/2014
CONCENTRATION: 34.2mg/ml
OBSERVED MW: 52kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MVLQIYNTLS KTKEVFTPLV GNQVRMYVCG MTVYDYCHLG HGRSMVAFDV ITRWLRHRGY DLTYVRNITD IDDKIINRAN ENGEPFDVLT ERMIAAMHED EARLNILKPD QEPRATDHIA GMHAMIQTLI DKGYAYAPGN GDVYYRVGKF AGYGKLSRRR VEDLRIGARI EPGEAKEDPL DFVLWKGAKP GEPSWSSPWG EGRPGWHIEC SVMSTCCLGD SFDIHGGGND LEFPHHENEI AQSEAATGKP YAKSWLHCGM ITINGEKMSK SLGNFFTIRE VLEKYHPEVV RYLLIASHYR SPINYSEENL REAKAALDRF YNALKGLPEA APAEAAEYVE RFAAAMDDDF NTAGACSVLF ELAREVNRLR ESDLSAAAAL AARLKQLAGL LGVLQLEPEA FLQAGAEGKV DAAEVEALIQ ARLEARAAKN WAESDRIRDQ LTAMGVVLED GK
Validated NT Sequence
tcaccaccac caccaccata tggtgctgca gatctacaac acgctgagca agaccaaaga agtcttcacg ccgctggtcg gcaaccaggt gcgcatgtac gtgtgcggca tgaccgtgta cgactactgc catctgggcc acggccgcag catggtggcg ttcgacgtga tcacccgctg gttgcgccac cgtggctacg acctgacgta cgtgcgcaac atcaccgata tcgatgacaa gatcatcaat cgcgccaacg agaacggcga accgttcgat gtgctgaccg agcgcatgat cgcggcgatg cacgaggacg aggcccggct gaacatcctc aagccggacc aggagccgcg tgccaccgat cacatcgccg gcatgcacgc gatgatccag accctgatcg acaagggcta tgcctacgcg ccgggcaacg gtgacgtgta ctaccgggtc ggcaagttcg ctggctacgg caagttgtcg cggcggcggg tcgaggacct gcgcatcggt gcgcggatcg aaccgggcga agcgaaggag gatccgctcg atttcgtgct ctggaagggc gccaagccgg gcgagccgag ctggtcgtcg ccctggggcg aggggcgccc gggctggcac atcgaatgct cggtgatgtc cacctgctgc ctgggcgatt ccttcgacat ccatggcggt ggcaacgacc tggagttccc tcaccacgag aacgagatcg cccagagcga ggccgcgacc ggcaagccct acgccaagtc ctggctgcac tgcgggatga tcaccatcaa cggcgagaag atgtccaagt cgctgggcaa tttcttcacc atccgcgaag tgctggagaa atatcacccc gaagtggtgc gttacctgct gatcgccagc cattaccgca gtccgatcaa ctactcggag gagaacctgc gcgaggccaa ggcggccctg gaccgtttct acaacgcgct caaggggctg cccgaggcgg cgccggcgga agcggcggag tatgtcgagc gcttcgccgc ggcgatggac gacgacttca ataccgcggg tgcctgctcg gtgctgttcg agctggcgcg ggaggtcaat cgtctgcgcg aaagcgacct gtcggcggcg gccgcgctgg ccgcgcgcct gaagcagttg gctggcctgc tgggggtgct gcaactggag ccggaagcct tcctgcaggc gggcgccgag ggcaaggtcg atgcggccga ggtcgaggcg ctgatccagg cgcgcctgga ggcgcgggcg gcgaagaact gggcggagtc cgaccggatt cgcgatcagc tcaccgccat gggcgtagtg ctggaggatg gcaagggc
Expressed Protein Sequence
MAHHHHHHML QIYNTLSKTK EVFTPLVGNQ VRMYVCGMTV YDYCHLGHGR SMVAFDVITR WLRHRGYDLT YVRNITDIDD KIINRANENG EPFDVLTERM IAAMHEDEAR LNILKPDQEP RATDHIAGMH AMIQTLIDKG YAYAPGNGDV YYRVGKFAGY GKLSRRRVED LRIGARIEPG EAKEDPLDFV LWKGAKPGEP SWSSPWGEGR PGWHIECSVM STCCLGDSFD IHGGGNDLEF PHHENEIAQS EAATGKPYAK SWLHCGMITI NGEKMSKSLG NFFTIREVLE KYHPEVVRYL LIASHYRSPI NYSEENLREA KAALDRFYNA LKGLPEAAPA EAAEYVERFA AAMDDDFNTA GACSVLFELA REVNRLRESD LSAAAALAAR LKQLAGLLGV LQLEPEAFLQ AGAEGKVDAA EVEALIQARL EARAAKNWAE SDRIRDQLTA MGVVLEDGKG GTTWRLAD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGCTGC AGATCTACAA CACGCTGAGC AAGACCAAAG AAGTCTTCAC GCCGCTGGTC GGCAACCAGG TGCGCATGTA CGTGTGCGGC ATGACCGTGT ACGACTACTG CCATCTGGGC CACGGCCGCA GCATGGTGGC GTTCGACGTG ATCACCCGCT GGTTGCGCCA CCGTGGCTAC GACCTGACGT ACGTGCGCAA CATCACCGAT ATCGATGACA AGATCATCAA TCGCGCCAAC GAGAACGGCG AACCGTTCGA TGTGCTGACC GAGCGCATGA TCGCGGCGAT GCACGAGGAC GAGGCCCGGC TGAACATCCT CAAGCCGGAC CAGGAGCCGC GTGCCACCGA TCACATCGCC GGCATGCACG CGATGATCCA GACCCTGATC GACAAGGGCT ATGCCTACGC GCCGGGCAAC GGTGACGTGT ACTACCGGGT CGGCAAGTTC GCTGGCTACG GCAAGTTGTC GCGGCGGCGG GTCGAGGACC TGCGCATCGG TGCGCGGATC GAACCGGGCG AAGCGAAGGA GGATCCGCTC GATTTCGTGC TCTGGAAGGG CGCCAAGCCG GGCGAGCCGA GCTGGTCGTC GCCCTGGGGC GAGGGGCGCC CGGGCTGGCA CATCGAATGC TCGGTGATGT CCACCTGCTG CCTGGGCGAT TCCTTCGACA TCCATGGCGG TGGCAACGAC CTGGAGTTCC CTCACCACGA GAACGAGATC GCCCAGAGCG AGGCCGCGAC CGGCAAGCCC TACGCCAAGT CCTGGCTGCA CTGCGGGATG ATCACCATCA ACGGCGAGAA GATGTCCAAG TCGCTGGGCA ATTTCTTCAC CATCCGCGAA GTGCTGGAGA AATATCACCC CGAAGTGGTG CGTTACCTGC TGATCGCCAG CCATTACCGC AGTCCGATCA ACTACTCGGA GGAGAACCTG CGCGAGGCCA AGGCGGCCCT GGACCGTTTC TACAACGCGC TCAAGGGGCT GCCCGAGGCG GCGCCGGCGG AAGCGGCGGA GTATGTCGAG CGCTTCGCCG CGGCGATGGA CGACGACTTC AATACCGCGG GTGCCTGCTC GGTGCTGTTC GAGCTGGCGC GGGAGGTCAA TCGTCTGCGC GAAAGCGACC TGTCGGCGGC GGCCGCGCTG GCCGCGCGCC TGAAGCAGTT GGCTGGCCTG CTGGGGGTGC TGCAACTGGA GCCGGAAGCC TTCCTGCAGG CGGGCGCCGA GGGCAAGGTC GATGCGGCCG AGGTCGAGGC GCTGATCCAG GCGCGCCTGG AGGCGCGGGC GGCGAAGAAC TGGGCGGAGT CCGACCGGAT TCGCGATCAG CTCACCGCCA TGGGCGTAGT GCTGGAGGAT GGCAAGGGCG GCACTACCTG GCGCCTGGCC GACTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for PsaeA.00133.a.B2.PW37945
PURIFICATION DATe: 10/17/2016
CONCENTRATION: 31mg/ml
OBSERVED MW: 51kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHML QIYNTLSKTK EVFTPLVGNQ VRMYVCGMTV YDYCHLGHGR SMVAFDVITR WLRHRGYDLT YVRNITDIDD KIINRANENG EPFDVLTERM IAAMHEDEAR LNILKPDQEP RATDHIAGMH AMIQTLIDKG YAYAPGNGDV YYRVGKFAGY GKLSRRRVED LRIGARIEPG EAKEDPLDFV LWKGAKPGEP SWSSPWGEGR PGWHIECSVM STCCLGDSFD IHGGGNDLEF PHHENEIAQS EAATGKPYAK SWLHCGMITI NGEKMSKSLG NFFTIREVLE KYHPEVVRYL LIASHYRSPI NYSEENLREA KAALDRFYNA LKGLPEAAPA EAAEYVERFA AAMDDDFNTA GACSVLFELA REVNRLRESD LSAAAALAAR LKQLAGLLGV LQLEPEAFLQ AGAEGKVDAA EVEALIQARL EARAAKNWAE SDRIRDQLTA MGVVLEDGKG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGCTGC AGATCTACAA CACGCTGAGC AAGACCAAAG AAGTCTTCAC GCCGCTGGTC GGCAACCAGG TGCGCATGTA CGTGTGCGGC ATGACCGTGT ACGACTACTG CCATCTGGGC CACGGCCGCA GCATGGTGGC GTTCGACGTG ATCACCCGCT GGTTGCGCCA CCGTGGCTAC GACCTGACGT ACGTGCGCAA CATCACCGAT ATCGATGACA AGATCATCAA TCGCGCCAAC GAGAACGGCG AACCGTTCGA TGTGCTGACC GAGCGCATGA TCGCGGCGAT GCACGAGGAC GAGGCCCGGC TGAACATCCT CAAGCCGGAC CAGGAGCCGC GTGCCACCGA TCACATCGCC GGCATGCACG CGATGATCCA GACCCTGATC GACAAGGGCT ATGCCTACGC GCCGGGCAAC GGTGACGTGT ACTACCGGGT CGGCAAGTTC GCTGGCTACG GCAAGTTGTC GCGGCGGCGG GTCGAGGACC TGCGCATCGG TGCGCGGATC GAACCGGGCG AAGCGAAGGA GGATCCGCTC GATTTCGTGC TCTGGAAGGG CGCCAAGCCG GGCGAGCCGA GCTGGTCGTC GCCCTGGGGC GAGGGGCGCC CGGGCTGGCA CATCGAATGC TCGGTGATGT CCACCTGCTG CCTGGGCGAT TCCTTCGACA TCCATGGCGG TGGCAACGAC CTGGAGTTCC CTCACCACGA GAACGAGATC GCCCAGAGCG AGGCCGCGAC CGGCAAGCCC TACGCCAAGT CCTGGCTGCA CTGCGGGATG ATCACCATCA ACGGCGAGAA GATGTCCAAG TCGCTGGGCA ATTTCTTCAC CATCCGCGAA GTGCTGGAGA AATATCACCC CGAAGTGGTG CGTTACCTGC TGATCGCCAG CCATTACCGC AGTCCGATCA ACTACTCGGA GGAGAACCTG CGCGAGGCCA AGGCGGCCCT GGACCGTTTC TACAACGCGC TCAAGGGGCT GCCCGAGGCG GCGCCGGCGG AAGCGGCGGA GTATGTCGAG CGCTTCGCCG CGGCGATGGA CGACGACTTC AATACCGCGG GTGCCTGCTC GGTGCTGTTC GAGCTGGCGC GGGAGGTCAA TCGTCTGCGC GAAAGCGACC TGTCGGCGGC GGCCGCGCTG GCCGCGCGCC TGAAGCAGTT GGCTGGCCTG CTGGGGGTGC TGCAACTGGA GCCGGAAGCC TTCCTGCAGG CGGGCGCCGA GGGCAAGGTC GATGCGGCCG AGGTCGAGGC GCTGATCCAG GCGCGCCTGG AGGCGCGGGC GGCGAAGAAC TGGGCGGAGT CCGACCGGAT TCGCGATCAG CTCACCGCCA TGGGCGTAGT GCTGGAGGAT GGCAAGGGCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT