PsaeA.00131.a
Pyridoxine 5-prime-phosphate synthase (PNP synthase) (EC 2.6.99.2)
| CENTER ID: | PsaeA.00131.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00131.a.B1.GE38200 | full length | 1 | 248 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00131.a.B1.PW37619 | full length | 1 | 248 |
Structures
5DLC
DEPOSITED: 9/4/2015
DETERMINATION: XRay
CLONE: PsaeA.00131.a.B1.GE38200
PROTEIN: PsaeA.00131.a.B1.PW37619
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.803 |
| PGAT: | pseudomonas:PA0773 |
| RefSeq: | NP_249464.1 |
| UniProt: | Q9I5G5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEATRILLG VNIDHVATLR QARGTRYPDP VKAALDAEEA GADGITVHLR EDRRHIQERD VRVLKEVLQT RMNFEMGVTE EMLAFAEEIR PAHSCLVPER REELTTEGGL DVAGQEQRIR DAVRRLAAVG SEVSLFIDPD PRQIEASARV GAPAIELHTG RYADAEDPEE QARELQRVRE GVALGRSLGL IVNAGHGLHY HNVEPVAAID GINELNIGHA IVAHALFVGF RQAVAEMKAL MLAAATKR
NT Sequence
gtgactgaag ccactcgaat tctgctcggc gtgaacatcg atcacgtcgc caccctgcgc caggcccggg gcacccgcta tccggacccg gtgaaggccg ccctggacgc cgaggaagcc ggcgccgacg gtatcaccgt gcacctgcgc gaggaccgcc ggcatatcca ggaacgcgac gtgcgggtgc tcaaggaagt gctgcagacg cggatgaact tcgagatggg ggtcaccgag gaaatgctcg ccttcgccga ggagatccgg ccggcgcata gctgcctggt ccccgagcgc cgcgaggaac tgaccaccga gggcggcctc gacgtcgccg gccaggaaca gcgcatccgc gacgcggtgc gacgcctggc tgcggtgggc agcgaagtgt cgctgttcat cgatcccgat ccgcggcaga tcgaggcctc cgcccgggtc ggcgcgccgg ctatcgaact gcataccggt cgctacgccg acgccgaaga ccccgaggag caggcccgcg agctgcagcg ggtacgcgag ggcgtggccc tcgggcgttc cctcggcctg atcgtcaacg ccgggcatgg cctgcactac cacaatgtcg agccggtcgc ggccatcgac ggcatcaacg agctgaacat cggccacgcc atcgtcgccc acgccctctt cgtcggcttc aggcaggcgg tggccgagat gaaagcgctg atgctggccg cggcgaccaa gcgctga
Details for PsaeA.00131.a.B1.GE38200
| HARVESTED ON: | 6/11/2014 |
| SEQUENCED ON: | 6/12/2014 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV TEATRILLGV NIDHVATLRQ ARGTRYPDPV KAALDAEEAG ADGITVHLRE DRRHIQERDV RVLKEVLQTR MNFEMGVTEE MLAFAEEIRP AHSCLVPERR EELTTEGGLD VAGQEQRIRD AVRRLAAVGS EVSLFIDPDP RQIEASARVG APAIELHTGR YADAEDPEEQ ARELQRVREG VALGRSLGLI VNAGHGLHYH NVEPVAAIDG INELNIGHAI VAHALFVGFR QAVAEMKALM LAAATKR
Validated NT Sequence
atcttactca cttagcgctt ggtcgccgcg gccagcatca gcgctttcat ctcggccacc gcctgcctga agccgacgaa gagggcgtgg gcgacgatgg cgtggccgat gttcagctcg ttgatgccgt cgatggccgc gaccggctcg acattgtggt agtgcaggcc atgcccggcg ttgacgatca ggccgaggga acgcccgagg gccacgccct cgcgtacccg ctgcagctcg cgggcctgct cctcggggtc ttcggcgtcg gcgtagcgac cggtatgcag ttcgatagcc ggcgcgccga cccgggcgga ggcctcgatc tgccgcggat cgggatcgat gaacagcgac acttcgctgc ccaccgcagc caggcgtcgc accgcgtcgc ggatgcgctg ttcctggccg gcgacgtcga ggccgccctc ggtggtcagt tcctcgcggc gctcggggac caggcagcta tgcgccggcc ggatctcctc ggcgaaggcg agcatttcct cggtgacccc catctcgaag ttcatccgcg tctgcagcac ttccttgagc acccgcacgt cgcgttcctg gatatgccgg cggtcctcgc gcaggtgcac ggtgataccg tcggcgccgg cttcctcggc gtccagggcg gccttcaccg ggtccggata gcgggtgccc cgggcctggc gcagggtggc gacgtgatcg atgttcacgc cgagcagaat tcgagtggct tcagtcacca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMT EATRILLGVN IDHVATLRQA RGTRYPDPVK AALDAEEAGA DGITVHLRED RRHIQERDVR VLKEVLQTRM NFEMGVTEEM LAFAEEIRPA HSCLVPERRE ELTTEGGLDV AGQEQRIRDA VRRLAAVGSE VSLFIDPDPR QIEASARVGA PAIELHTGRY ADAEDPEEQA RELQRVREGV ALGRSLGLIV NAGHGLHYHN VEPVAAIDGI NELNIGHAIV AHALFVGFRQ AVAEMKALML AAATKR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgactg aagccactcg aattctgctc ggcgtgaaca tcgatcacgt cgccaccctg cgccaggccc ggggcacccg ctatccggac ccggtgaagg ccgccctgga cgccgaggaa gccggcgccg acggtatcac cgtgcacctg cgcgaggacc gccggcatat ccaggaacgc gacgtgcggg tgctcaagga agtgctgcag acgcggatga acttcgagat gggggtcacc gaggaaatgc tcgccttcgc cgaggagatc cggccggcgc atagctgcct ggtccccgag cgccgcgagg aactgaccac cgagggcggc ctcgacgtcg ccggccagga acagcgcatc cgcgacgcgg tgcgacgcct ggctgcggtg ggcagcgaag tgtcgctgtt catcgatccc gatccgcggc agatcgaggc ctccgcccgg gtcggcgcgc cggctatcga actgcatacc ggtcgctacg ccgacgccga agaccccgag gagcaggccc gcgagctgca gcgggtacgc gagggcgtgg ccctcgggcg ttccctcggc ctgatcgtca acgccgggca tggcctgcac taccacaatg tcgagccggt cgcggccatc gacggcatca acgagctgaa catcggccac gccatcgtcg cccacgccct cttcgtcggc ttcaggcagg cggtggccga gatgaaagcg ctgatgctgg ccgcggcgac caagcgctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for PsaeA.00131.a.B1.PW37619
| PURIFICATION DATe: | 12/17/2014 |
| CONCENTRATION: | 5mg/ml |
| OBSERVED MW: | 29kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 24 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV TEATRILLGV NIDHVATLRQ ARGTRYPDPV KAALDAEEAG ADGITVHLRE DRRHIQERDV RVLKEVLQTR MNFEMGVTEE MLAFAEEIRP AHSCLVPERR EELTTEGGLD VAGQEQRIRD AVRRLAAVGS EVSLFIDPDP RQIEASARVG APAIELHTGR YADAEDPEEQ ARELQRVREG VALGRSLGLI VNAGHGLHYH NVEPVAAIDG INELNIGHAI VAHALFVGFR QAVAEMKALM LAAATKR
Validated NT Sequence
atcttactca cttagcgctt ggtcgccgcg gccagcatca gcgctttcat ctcggccacc gcctgcctga agccgacgaa gagggcgtgg gcgacgatgg cgtggccgat gttcagctcg ttgatgccgt cgatggccgc gaccggctcg acattgtggt agtgcaggcc atgcccggcg ttgacgatca ggccgaggga acgcccgagg gccacgccct cgcgtacccg ctgcagctcg cgggcctgct cctcggggtc ttcggcgtcg gcgtagcgac cggtatgcag ttcgatagcc ggcgcgccga cccgggcgga ggcctcgatc tgccgcggat cgggatcgat gaacagcgac acttcgctgc ccaccgcagc caggcgtcgc accgcgtcgc ggatgcgctg ttcctggccg gcgacgtcga ggccgccctc ggtggtcagt tcctcgcggc gctcggggac caggcagcta tgcgccggcc ggatctcctc ggcgaaggcg agcatttcct cggtgacccc catctcgaag ttcatccgcg tctgcagcac ttccttgagc acccgcacgt cgcgttcctg gatatgccgg cggtcctcgc gcaggtgcac ggtgataccg tcggcgccgg cttcctcggc gtccagggcg gccttcaccg ggtccggata gcgggtgccc cgggcctggc gcagggtggc gacgtgatcg atgttcacgc cgagcagaat tcgagtggct tcagtcacca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMT EATRILLGVN IDHVATLRQA RGTRYPDPVK AALDAEEAGA DGITVHLRED RRHIQERDVR VLKEVLQTRM NFEMGVTEEM LAFAEEIRPA HSCLVPERRE ELTTEGGLDV AGQEQRIRDA VRRLAAVGSE VSLFIDPDPR QIEASARVGA PAIELHTGRY ADAEDPEEQA RELQRVREGV ALGRSLGLIV NAGHGLHYHN VEPVAAIDGI NELNIGHAIV AHALFVGFRQ AVAEMKALML AAATKR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGACTG AAGCCACTCG AATTCTGCTC GGCGTGAACA TCGATCACGT CGCCACCCTG CGCCAGGCCC GGGGCACCCG CTATCCGGAC CCGGTGAAGG CCGCCCTGGA CGCCGAGGAA GCCGGCGCCG ACGGTATCAC CGTGCACCTG CGCGAGGACC GCCGGCATAT CCAGGAACGC GACGTGCGGG TGCTCAAGGA AGTGCTGCAG ACGCGGATGA ACTTCGAGAT GGGGGTCACC GAGGAAATGC TCGCCTTCGC CGAGGAGATC CGGCCGGCGC ATAGCTGCCT GGTCCCCGAG CGCCGCGAGG AACTGACCAC CGAGGGCGGC CTCGACGTCG CCGGCCAGGA ACAGCGCATC CGCGACGCGG TGCGACGCCT GGCTGCGGTG GGCAGCGAAG TGTCGCTGTT CATCGATCCC GATCCGCGGC AGATCGAGGC CTCCGCCCGG GTCGGCGCGC CGGCTATCGA ACTGCATACC GGTCGCTACG CCGACGCCGA AGACCCCGAG GAGCAGGCCC GCGAGCTGCA GCGGGTACGC GAGGGCGTGG CCCTCGGGCG TTCCCTCGGC CTGATCGTCA ACGCCGGGCA TGGCCTGCAC TACCACAATG TCGAGCCGGT CGCGGCCATC GACGGCATCA ACGAGCTGAA CATCGGCCAC GCCATCGTCG CCCACGCCCT CTTCGTCGGC TTCAGGCAGG CGGTGGCCGA GATGAAAGCG CTGATGCTGG CCGCGGCGAC CAAGCGCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT