PsaeA.00111.a: Glutamine synthetase (EC 6.3.1.2) (Glutamate--ammonia ligase)
Target Characteristics
| from organism: | Pseudomonas aeruginosa PAO1 |
| most recent status: | crystallized |
| center reference id: | PsaeA.00111.a |
| is community request: | True |
| associated disease: | Pseudomonas infection |
| NIH risk group: | unclassified |
| is select agent: | False |
| NIH priority pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00111.a.A1.GE44582 | full length | 1 | 469 | GE44582 | |
| PsaeA.00111.a.AE1.GE44724 | full length | 1 | 469 | GE44724 | |
| PsaeA.00111.a.B1.GE38199 | full length | 1 | 469 | GE38199 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
External Resources
| Resource | Reference ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.5365 |
| PGAT: | pseudomonas:PA5119 |
| RefSeq: | NP_253806.1 |
| UniProt: | Q9HU65 |
Enzyme & Pathway Information
| Pathway | Pathway ID | EC Number |
|---|---|---|
| ammonia assimilation cycle III | AMMASSIM-PWY | 6.3.1.2 |
| L-glutamine biosynthesis I | GLNSYN-PWY | 6.3.1.2 |
| superpathway of ammonia assimilation (plants) | PWY-3282 | 6.3.1.2 |
| nitrate reduction II (assimilatory) | PWY-381 | 6.3.1.2 |
| nitrate reduction VI (assimilatory) | PWY490-3 | 6.3.1.2 |
| L-glutamate and L-glutamine biosynthesis | PWY-5505 | 6.3.1.2 |
| nitrate reduction V (assimilatory) | PWY-5675 | 6.3.1.2 |
| L-glutamine biosynthesis III | PWY-6549 | 6.3.1.2 |
| ammonia assimilation cycle I | PWY-6963 | 6.3.1.2 |
| ammonia assimilation cycle II | PWY-6964 | 6.3.1.2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MSYKSHQLIK DHDVKWVDLR FTDTKGKQQH VTMPARDALD DEFFEAGKMF DGSSIAGWKG IEASDMILMP DDSTAVLDPF TEEPTLILVC DIIEPSTMQG YERDPRNIAK RAEEYLKSTG IGDTVFVGPE PEFFIFDEVK FKSDISGSMF KIFSEQASWN TDADIESGNK GHRPGVKGGY FPVPPVDHDH EIRTAMCNAL EEMGLVVEVH HHEVATAGQN EIGVKFNTLV AKADEVQTLK YCVHNVADAY GKTVTFMPKP LYGDNGSGMH VHMSISKDGK NTFAGEGYAG LSETALYFIG GIIKHGKALN GFTNPSTNSY KRLVPGFEAP VMLAYSARNR SASIRIPYVS SPKARRIEAR FPDPAANPYL AFAALLMAGL DGIQNKIHPG DAADKNLYDL PPEEAKEIPQ VCGSLKEALE ELDKGRAFLT KGGVFTDEFI DAYIELKSEE EIKVRTFVHP LEYDLYYSV
NT Sequence
atgtcgtaca agtcgcacca actgatcaaa gaccatgacg tgaagtgggt agacctgcgc ttcaccgata ccaagggcaa gcagcaacac gtcaccatgc cggctcgcga cgcgctggac gatgagttct tcgaagccgg caagatgttc gacggctcct ccatcgccgg ctggaaaggc atcgaagcct ccgacatgat cctgatgccg gacgacagca ccgccgtcct cgatccgttc accgaagagc cgaccctgat cctggtctgc gacatcatcg agccgagcac catgcaaggc tacgagcgcg acccgcgcaa catcgccaag cgcgccgagg agtacctgaa gtccaccggc atcggcgaca ccgtgttcgt cggcccggag ccggagttct tcatcttcga cgaagtgaag ttcaagtccg acatctccgg ctcgatgttc aagatcttct ccgagcaggc ttcctggaac accgacgccg acatcgagtc cggcaacaag ggccatcgcc cgggcgtgaa gggcggctac ttcccggtac cgccggtcga ccacgaccat gaaatccgca ccgccatgtg caacgccctg gaagaaatgg gcctggtggt cgaagtccac caccacgaag tggcaaccgc cggccagaac gagatcggcg tgaagttcaa caccctggtc gccaaggccg acgaagtgca gaccctgaag tactgcgtgc acaacgtcgc cgacgcctac ggcaagaccg tgaccttcat gccgaagccg ctgtacggcg acaacggttc gggcatgcac gtgcacatgt cgatctccaa ggacggcaag aacaccttcg ccggcgaagg ctatgccggc ctgtccgaga ccgccctgta cttcatcggc ggcatcatca agcacggcaa ggccctgaac ggcttcacca acccctcgac caactcctac aagcgcctgg tcccgggctt cgaagctccg gtgatgctgg cctactcggc gcgcaaccgt tccgcctcga tccgtatccc ctacgtatcc agcccgaagg cccgccgtat cgaagcgcgc ttcccggacc cggcagccaa cccctacctg gccttcgccg cgctgctgat ggccggcctg gacggcatcc agaacaagat ccaccccggc gatgccgccg acaagaacct gtacgacctg ccgccggaag aggcgaagga aatcccgcag gtttgcggca gcctgaaaga ggcgctggaa gaactcgaca agggccgcgc gttcctgacc aagggcggcg tgttcaccga cgagttcatc gatgcctaca tcgagctgaa gagcgaagaa gagatcaagg tgcgcacctt cgtgcacccg ctggaatacg acctgtacta cagcgtctga