PsaeA.00111.a

Glutamine synthetase (EC 6.3.1.2) (Glutamate--ammonia ligase)

CENTER ID: PsaeA.00111.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00111.a.A1.GE44582 full length 1 469
PsaeA.00111.a.AE1.GE44724 full length 1 469
PsaeA.00111.a.B1.GE38199 full length 1 469
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|208964.12.peg.5365
PGAT: pseudomonas:PA5119
RefSeq: NP_253806.1
UniProt: Q9HU65
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSYKSHQLIK DHDVKWVDLR FTDTKGKQQH VTMPARDALD DEFFEAGKMF DGSSIAGWKG IEASDMILMP DDSTAVLDPF TEEPTLILVC DIIEPSTMQG YERDPRNIAK RAEEYLKSTG IGDTVFVGPE PEFFIFDEVK FKSDISGSMF KIFSEQASWN TDADIESGNK GHRPGVKGGY FPVPPVDHDH EIRTAMCNAL EEMGLVVEVH HHEVATAGQN EIGVKFNTLV AKADEVQTLK YCVHNVADAY GKTVTFMPKP LYGDNGSGMH VHMSISKDGK NTFAGEGYAG LSETALYFIG GIIKHGKALN GFTNPSTNSY KRLVPGFEAP VMLAYSARNR SASIRIPYVS SPKARRIEAR FPDPAANPYL AFAALLMAGL DGIQNKIHPG DAADKNLYDL PPEEAKEIPQ VCGSLKEALE ELDKGRAFLT KGGVFTDEFI DAYIELKSEE EIKVRTFVHP LEYDLYYSV
NT Sequence
atgtcgtaca agtcgcacca actgatcaaa gaccatgacg tgaagtgggt agacctgcgc ttcaccgata ccaagggcaa gcagcaacac gtcaccatgc cggctcgcga cgcgctggac gatgagttct tcgaagccgg caagatgttc gacggctcct ccatcgccgg ctggaaaggc atcgaagcct ccgacatgat cctgatgccg gacgacagca ccgccgtcct cgatccgttc accgaagagc cgaccctgat cctggtctgc gacatcatcg agccgagcac catgcaaggc tacgagcgcg acccgcgcaa catcgccaag cgcgccgagg agtacctgaa gtccaccggc atcggcgaca ccgtgttcgt cggcccggag ccggagttct tcatcttcga cgaagtgaag ttcaagtccg acatctccgg ctcgatgttc aagatcttct ccgagcaggc ttcctggaac accgacgccg acatcgagtc cggcaacaag ggccatcgcc cgggcgtgaa gggcggctac ttcccggtac cgccggtcga ccacgaccat gaaatccgca ccgccatgtg caacgccctg gaagaaatgg gcctggtggt cgaagtccac caccacgaag tggcaaccgc cggccagaac gagatcggcg tgaagttcaa caccctggtc gccaaggccg acgaagtgca gaccctgaag tactgcgtgc acaacgtcgc cgacgcctac ggcaagaccg tgaccttcat gccgaagccg ctgtacggcg acaacggttc gggcatgcac gtgcacatgt cgatctccaa ggacggcaag aacaccttcg ccggcgaagg ctatgccggc ctgtccgaga ccgccctgta cttcatcggc ggcatcatca agcacggcaa ggccctgaac ggcttcacca acccctcgac caactcctac aagcgcctgg tcccgggctt cgaagctccg gtgatgctgg cctactcggc gcgcaaccgt tccgcctcga tccgtatccc ctacgtatcc agcccgaagg cccgccgtat cgaagcgcgc ttcccggacc cggcagccaa cccctacctg gccttcgccg cgctgctgat ggccggcctg gacggcatcc agaacaagat ccaccccggc gatgccgccg acaagaacct gtacgacctg ccgccggaag aggcgaagga aatcccgcag gtttgcggca gcctgaaaga ggcgctggaa gaactcgaca agggccgcgc gttcctgacc aagggcggcg tgttcaccga cgagttcatc gatgcctaca tcgagctgaa gagcgaagaa gagatcaagg tgcgcacctt cgtgcacccg ctggaatacg acctgtacta cagcgtctga
Details for PsaeA.00111.a.A1.GE44582
HARVESTED ON: 11/22/2021
SEQUENCED ON: 11/17/2021
EXPECTED MW: 54kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Data Unavailable
SOLUBLE EXPRESSION LEVEL Data Unavailable
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MSYKSHQLIK DHDVKWVDLR FTDTKGKQQH VTMPARDALD DEFFEAGKMF DGSSIAGWKG IEASDMILMP DDSTAVLDPF TEEPTLILVC DIIEPSTMQG YERDPRNIAK RAEEYLKSTG IGDTVFVGPE PEFFIFDEVK FKSDISGSMF KIFSEQASWN TDADIESGNK GHRPGVKGGY FPVPPVDHDH EIRTAMCNAL EEMGLVVEVH HHEVATAGQN EIGVKFNTLV AKADEVQTLK YCVHNVAXAY GKTVTFMPKP LYGDNGSGMH VHMSISKDGK NTFAGEGYAG LSETALYFIG GIIKHGKALN GFTNPSTNSY KRLVPGFEAP VMLAYSARNR SASIRIPYVS SPKARRIEAR FPDPAANPYL AFAALLMAGL DGIQNKIHPG DAADKNLYDL PPEEAKEIPQ VCGSLKEALE ELDKGRAFLT KGGVFTDEFI DAYIELKSEE EIKVRTFVHP LEYDLYYSV
Validated NT Sequence
ttattagacg ctgtagtaca ggtcgtattc cagcgggtgc acgaaggtgc gcaccttgat ctcttcttcg ctcttcagct cgatgtaggc atcgatgaac tcgtcggtga acacgccgcc cttggtcagg aacgcgcggc ccttgtcgag ttcttccagc gcctctttca ggctgccgca aacctgcggg atttccttcg cctcttccgg cggcaggtcg tacaggttct tgtcggcggc atcgccgggg tggatcttgt tctggatgcc gtccaggccg gccatcagca gcgcggcgaa ggccaggtag gggttggctg ccgggtccgg gaagcgcgct tcgatacggc gggccttcgg gctggatacg taggggatac ggatcgaggc ggaacggttg cgcgccgagt aggccagcat caccggagct tcgaagcccg ggaccaggcg cttgtaggag ttggtcgagg ggttggtgaa gccgttcagg gccttgccgt gcttgatgat gccgccgatg aagtacaggg cggtctcgga caggccggca tagccttcgc cggcgaaggt gttcttgccg tccttggaga tcgacatgtg cacgtgcatg cccgaaccgt tgtcgccgta cagcggcttc ggcatgaang tcacggtctt gccgtaggcg tnggcgacgt tgtgcacgca gtacttcagg gtctgcactt cgtcggcctt ggcgaccagg gtgttgaact tcacgccgat ctcgttctgg ccggcggttg ccacttcgtg gtggtggact tcgaccacca ggcccatttc ttccagggcg ttgcacatgg cggtgcggat ttcatggtcg tggtcgaccg gcggtaccgg gaagtagccg cccttcacgc ccgggcgatg gcccttgttg ccggactcga tgtcggcgtc ggtgttccag gaagcctgct cggagaagat cttgaacatc gagccggaga tgtcggactt gaacttcact tcgtcgaaga tgaagaactc cggctccggg ccgacgaaca cggtgtcgcc gatgccggtg gacttcaggt actcctcggc gcgcttggcg atgttgcgcg ggtcgcgctc gtagccttgc atggtgctcg gctcgatgat gtcgcagacc aggatcaggg tcggctcttc ggtgaacgga tcgaggacgg cggtgctgtc gtccggcatc aggatcatgt cggaggcttc gatgcctttc cagccggcga tggaggagcc gtcgaacatc ttgccggctt cgaagaactc atcgtccagc gcgtcgcgag ccggcatggt gacgtgttgc tgcttgccct tggtatcggt gaagcgcagg tctacccact tcacgtcatg gtctttgatc agttggtgcg acttgtacga catcgaacca ggaccctggg tctgagcttc cagggtaccc a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSYKSHQLI KDHDVKWVDL RFTDTKGKQQ HVTMPARDAL DDEFFEAGKM FDGSSIAGWK GIEASDMILM PDDSTAVLDP FTEEPTLILV CDIIEPSTMQ GYERDPRNIA KRAEEYLKST GIGDTVFVGP EPEFFIFDEV KFKSDISGSM FKIFSEQASW NTDADIESGN KGHRPGVKGG YFPVPPVDHD HEIRTAMCNA LEEMGLVVEV HHHEVATAGQ NEIGVKFNTL VAKADEVQTL KYCVHNVADA YGKTVTFMPK PLYGDNGSGM HVHMSISKDG KNTFAGEGYA GLSETALYFI GGIIKHGKAL NGFTNPSTNS YKRLVPGFEA PVMLAYSARN RSASIRIPYV SSPKARRIEA RFPDPAANPY LAFAALLMAG LDGIQNKIHP GDAADKNLYD LPPEEAKEIP QVCGSLKEAL EELDKGRAFL TKGGVFTDEF IDAYIELKSE EEIKVRTFVH PLEYDLYYSV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgtacaag tcgcaccaac tgatcaaaga ccatgacgtg aagtgggtag acctgcgctt caccgatacc aagggcaagc agcaacacgt caccatgccg gctcgcgacg cgctggacga tgagttcttc gaagccggca agatgttcga cggctcctcc atcgccggct ggaaaggcat cgaagcctcc gacatgatcc tgatgccgga cgacagcacc gccgtcctcg atccgttcac cgaagagccg accctgatcc tggtctgcga catcatcgag ccgagcacca tgcaaggcta cgagcgcgac ccgcgcaaca tcgccaagcg cgccgaggag tacctgaagt ccaccggcat cggcgacacc gtgttcgtcg gcccggagcc ggagttcttc atcttcgacg aagtgaagtt caagtccgac atctccggct cgatgttcaa gatcttctcc gagcaggctt cctggaacac cgacgccgac atcgagtccg gcaacaaggg ccatcgcccg ggcgtgaagg gcggctactt cccggtaccg ccggtcgacc acgaccatga aatccgcacc gccatgtgca acgccctgga agaaatgggc ctggtggtcg aagtccacca ccacgaagtg gcaaccgccg gccagaacga gatcggcgtg aagttcaaca ccctggtcgc caaggccgac gaagtgcaga ccctgaagta ctgcgtgcac aacgtcgccg acgcctacgg caagaccgtg accttcatgc cgaagccgct gtacggcgac aacggttcgg gcatgcacgt gcacatgtcg atctccaagg acggcaagaa caccttcgcc ggcgaaggct atgccggcct gtccgagacc gccctgtact tcatcggcgg catcatcaag cacggcaagg ccctgaacgg cttcaccaac ccctcgacca actcctacaa gcgcctggtc ccgggcttcg aagctccggt gatgctggcc tactcggcgc gcaaccgttc cgcctcgatc cgtatcccct acgtatccag cccgaaggcc cgccgtatcg aagcgcgctt cccggacccg gcagccaacc cctacctggc cttcgccgcg ctgctgatgg ccggcctgga cggcatccag aacaagatcc accccggcga tgccgccgac aagaacctgt acgacctgcc gccggaagag gcgaaggaaa tcccgcaggt ttgcggcagc ctgaaagagg cgctggaaga actcgacaag ggccgcgcgt tcctgaccaa gggcggcgtg ttcaccgacg agttcatcga tgcctacatc gagctgaaga gcgaagaaga gatcaaggtg cgcaccttcg tgcacccgct ggaatacgac ctgtactaca gcgtctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for PsaeA.00111.a.AE1.GE44724
HARVESTED ON: 3/21/2022
SEQUENCED ON: 3/22/2022
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MSYKSHQLIK DHDVKWVDLR FTDTKGKQQH VTMPARDALD DEFFEAGKMF DGSSIAGWKG IEASDMILMP DDSTAVLDPF TEEPTLILVC DIIEPSTMQG YERDPRNIAK RAEEYLKSTG IGDTVFVGPE PEFFIFDEVK FKSDISGSMF KIFSEQASWN TDADIESGNK GHRPGVKGGY FPVPPVDHDH EIRTAMCNAL EEMGLVVEVH HHEVATAGQN EIGVKFNTLV AKADEVQTLK YCVHNVADAY GKTVTFMPKP LYGDNGSGMH VHMSISKDGK NTFAGEGYAG LSETALYFIG GIIKHGKALN GFTNPSTNSY KRLVPGFEAP VMLAYSARNR SASIRIPYVS SPKARRIEAR FPDPAANPYL AFAALLMAGL DGIQNKIHPG DAADKNLYDL PPEEAKEIPQ VCGSLKEALE ELDKGRAFLT KGGVFTDEFI DAYIELKSEE EIKVRTFVHP LEYDLYYSVG HHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gtcgtacaag tcgcaccaac tgatcaaaga ccatgacgtg aagtgggtag acctgcgctt caccgatacc aagggcaagc agcaacacgt caccatgccg gctcgcgacg cgctggacga tgagttcttc gaagccggca agatgttcga cggctcctcc atcgccggct ggaaaggcat cgaagcctcc gacatgatcc tgatgccgga cgacagcacc gccgtcctcg atccgttcac cgaagagccg accctgatcc tggtctgcga catcatcgag ccgagcacca tgcaaggcta cgagcgcgac ccgcgcaaca tcgccaagcg cgccgaggag tacctgaagt ccaccggcat cggcgacacc gtgttcgtcg gcccggagcc ggagttcttc atcttcgacg aagtgaagtt caagtccgac atctccggct cgatgttcaa gatcttctcc gagcaggctt cctggaacac cgacgccgac atcgagtccg gcaacaaggg ccatcgcccg ggcgtgaagg gcggctactt cccggtaccg ccggtcgacc acgaccatga aatccgcacc gccatgtgca acgccctgga agaaatgggc ctggtggtcg aagtccacca ccacgaagtg gcaaccgccg gccagaacga gatcggcgtg aagttcaaca ccctggtcgc caaggccgac gaagtgcaga ccctgaagta ctgcgtgcac aacgtcgccg acgcctacgg caagaccgtg accttcatgc cgaagccgct gtacggcgac aacggttcgg gcatgcacgt gcacatgtcg atctccaagg acggcaagaa caccttcgcc ggcgaaggct atgccggcct gtccgagacc gccctgtact tcatcggcgg catcatcaag cacggcaagg ccctgaacgg cttcaccaac ccctcgacca actcctacaa gcgcctggtc ccgggcttcg aagctccggt gatgctggcc tactcggcgc gcaaccgttc cgcctcgatc cgtatcccct acgtatccag cccgaaggcc cgccgtatcg aagcgcgctt cccggacccg gcagccaacc cctacctggc cttcgccgcg ctgctgatgg ccggcctgga cggcatccag aacaagatcc accccggcga tgccgccgac aagaacctgt acgacctgcc gccggaagag gcgaaggaaa tcccgcaggt ttgcggcagc ctgaaagagg cgctggaaga actcgacaag ggccgcgcgt tcctgaccaa gggcggcgtg ttcaccgacg agttcatcga tgcctacatc gagctgaaga gcgaagaaga gatcaaggtg cgcaccttcg tgcacccgct ggaatacgac ctgtactaca gcgtcgggca ccaccatcat catcattaac ggatccgaat tcgagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gat
Details for PsaeA.00111.a.B1.GE38199
HARVESTED ON: 6/11/2014
SEQUENCED ON: 6/12/2014
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 91
Validated AA Sequence
MPARDALDDE FFEAGKMFDG SSIAGWKGIE ASDMILMPDD STAVLDPFTE EPTLILVCDI IEPSTMQGYE RDPRNIAKRA EEYLKSTGIG DTVFVGPEPE FFIFDEVKFK SDISGSMFKI FSEQASWNTD ADIESGNKGH RPGVKGGYFP VPPVDHDHEI RTAMCNALEE MGLVVEVHHH EVATAGQNEI GVKFNTLVAK ADEVQTLKYC VHNVADAYGK TVTFMPKPLY GDNGSGMHVH MSISKDGKNT FAGEGYAGLS ETALYFIGGI IKHGKALNGF TNPSTNSYKR LVPGFEAPVM LAYSARNRSA SIRIPYVSSP KARRIEARFP DPAANPYLAF AALLMAGLDG IQNKIHPGDA ADKNLYDLPP EEAKEIPQVC GSLKEALEEL DKGRAFLTKG GVFTDEFIDA YIELKSEEEI KVRTXXPPXE YDLYYSV
Validated NT Sequence
atcttactca cttagacgct gtagtacagg tcgtattcnn ncggngggan nnaggtgcgc accttgatct cttcttcgct cttcagctcg atgtaggcat cgatgaactc gtcggtgaac acgccgccct tggtcaggaa cgcgcggccc ttgtcgagtt cttccagcgc ctctttcagg ctgccgcaaa cctgcgggat ttccttcgcc tcttccggcg gcaggtcgta caggttcttg tcggcggcat cgccggggtg gatcttgttc tggatgccgt ccaggccggc catcagcagc gcggcgaagg ccaggtaggg gttggctgcc gggtccggga agcgcgcttc gatacggcgg gccttcgggc tggatacgta ggggatacgg atcgaggcgg aacggttgcg cgccgagtag gccagcatca ccggagcttc gaagcccggg accaggcgct tgtaggagtt ggtcgagggg ttggtgaagc cgttcagggc cttgccgtgc ttgatgatgc cgccgatgaa gtacagggcg gtctcggaca ggccggcata gccttcgccg gcgaaggtgt tcttgccgtc cttggagatc gacatgtgca cgtgcatgcc cgaaccgttg tcgccgtaca gcggcttcgg catgaaggtc acggtcttgc cgtaggcgtc ggcgacgttg tgcacgcagt acttcagggt ctgcacttcg tcggccttgg cgaccagggt gttgaacttc acgccgatct cgttctggcc ggcggttgcc acttcgtggt ggtggacttc gaccaccagg cccatttctt ccagggcgtt gcacatggcg gtgcggattt catggtcgtg gtcgaccggc ggtaccggga agtagccgcc cttcacgccc gggcgatggc ccttgttgcc ggactcgatg tcggcgtcgg tgttccagga agcctgctcg gagaagatct tgaacatcga gccggagatg tcggacttga acttcacttc gtcgaagatg aagaactccg gctccgggcc gacgaacacg gtgtcgccga tgccggtgga cttcaggtac tcctcggcgc gcttggcgat gttgcgcggg tcgcgctcgt agccttgcat ggtgctcggc tcgatgatgt cgcagaccag gatcagggtc ggctcttcgg tgaacggatc gaggacggcg gtgctgtcgt ccggcatcag gatcatgtcg gaggcttcga tgcctttcca gccggcgatg gaggagccgt cgaacatctt gccggcttcg aagaactcat cgtccagcgc gtcgcgagcc ggcatggtga cgtgttgctg cttgcccttg gtatcggtga agcgcaggtc tacccacttc acgtcatggt ctttgatcag ttggtgcgac ttgtacgac
Expected Protein Sequence
MAHHHHHHMS YKSHQLIKDH DVKWVDLRFT DTKGKQQHVT MPARDALDDE FFEAGKMFDG SSIAGWKGIE ASDMILMPDD STAVLDPFTE EPTLILVCDI IEPSTMQGYE RDPRNIAKRA EEYLKSTGIG DTVFVGPEPE FFIFDEVKFK SDISGSMFKI FSEQASWNTD ADIESGNKGH RPGVKGGYFP VPPVDHDHEI RTAMCNALEE MGLVVEVHHH EVATAGQNEI GVKFNTLVAK ADEVQTLKYC VHNVADAYGK TVTFMPKPLY GDNGSGMHVH MSISKDGKNT FAGEGYAGLS ETALYFIGGI IKHGKALNGF TNPSTNSYKR LVPGFEAPVM LAYSARNRSA SIRIPYVSSP KARRIEARFP DPAANPYLAF AALLMAGLDG IQNKIHPGDA ADKNLYDLPP EEAKEIPQVC GSLKEALEEL DKGRAFLTKG GVFTDEFIDA YIELKSEEEI KVRTFVHPLE YDLYYSV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgt acaagtcgca ccaactgatc aaagaccatg acgtgaagtg ggtagacctg cgcttcaccg ataccaaggg caagcagcaa cacgtcacca tgccggctcg cgacgcgctg gacgatgagt tcttcgaagc cggcaagatg ttcgacggct cctccatcgc cggctggaaa ggcatcgaag cctccgacat gatcctgatg ccggacgaca gcaccgccgt cctcgatccg ttcaccgaag agccgaccct gatcctggtc tgcgacatca tcgagccgag caccatgcaa ggctacgagc gcgacccgcg caacatcgcc aagcgcgccg aggagtacct gaagtccacc ggcatcggcg acaccgtgtt cgtcggcccg gagccggagt tcttcatctt cgacgaagtg aagttcaagt ccgacatctc cggctcgatg ttcaagatct tctccgagca ggcttcctgg aacaccgacg ccgacatcga gtccggcaac aagggccatc gcccgggcgt gaagggcggc tacttcccgg taccgccggt cgaccacgac catgaaatcc gcaccgccat gtgcaacgcc ctggaagaaa tgggcctggt ggtcgaagtc caccaccacg aagtggcaac cgccggccag aacgagatcg gcgtgaagtt caacaccctg gtcgccaagg ccgacgaagt gcagaccctg aagtactgcg tgcacaacgt cgccgacgcc tacggcaaga ccgtgacctt catgccgaag ccgctgtacg gcgacaacgg ttcgggcatg cacgtgcaca tgtcgatctc caaggacggc aagaacacct tcgccggcga aggctatgcc ggcctgtccg agaccgccct gtacttcatc ggcggcatca tcaagcacgg caaggccctg aacggcttca ccaacccctc gaccaactcc tacaagcgcc tggtcccggg cttcgaagct ccggtgatgc tggcctactc ggcgcgcaac cgttccgcct cgatccgtat cccctacgta tccagcccga aggcccgccg tatcgaagcg cgcttcccgg acccggcagc caacccctac ctggccttcg ccgcgctgct gatggccggc ctggacggca tccagaacaa gatccacccc ggcgatgccg ccgacaagaa cctgtacgac ctgccgccgg aagaggcgaa ggaaatcccg caggtttgcg gcagcctgaa agaggcgctg gaagaactcg acaagggccg cgcgttcctg accaagggcg gcgtgttcac cgacgagttc atcgatgcct acatcgagct gaagagcgaa gaagagatca aggtgcgcac cttcgtgcac ccgctggaat acgacctgta ctacagcgtc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat