SSGCID: Target Page

PsaeA.00085.a

Probable epimerase

CENTER ID:	PsaeA.00085.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.00085.a.B1.GE38197	full length		1	309

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.00085.a.B1.PS02118	full length		1	309
PsaeA.00085.a.B1.PS02192	full length		1	309

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.4260
PGAT:	pseudomonas:PA4068
RefSeq:	NP_252757.1
UniProt:	Q9HWW0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFERPILITG GAGFIGSHLT DALLARGYRV RVLDNLSTGK RDNLPLDDER VELLEGDAAD AATLAAALKG CAAVAHLAAV ASVQASVDDP VATHQSNFIA TLNLCEAMRE QGVKRVVFAS SAAVYGQNGE GTAIDEDTPK SPLTPYASDK LASEYYLDFY RRQHGLEPAI FRFFNVYGPR QDPSSPYSGV ISIFTERALK GTPITVFGDG EQTRDFIYVA DLVDFLVQAL EATAVEPGAV NVGLNRSISL KQLLAEIGQV LGGLPPVTHA DARAGDIRHS RANNERLLRR FRFDQPTPMG VGLARLLGR

NT Sequence

atgtttgaac gccccatcct gatcaccggc ggcgccggtt tcatcggctc ccacctgacc gacgcgctgc tggcgcgcgg ctaccgcgtg cgtgtgctgg acaacctgtc caccggcaag cgcgacaacc tgcccctgga cgacgaacgg gtagagctgc tcgaaggcga cgccgccgac gcggcgaccc tggcggccgc cctgaagggc tgcgcggcgg tcgcccacct ggccgcggtc gcctcggtac aggcctcggt ggacgacccg gtcgccaccc accagagcaa tttcatcgcc accctgaacc tctgcgaagc gatgcgcgag cagggcgtga agcgcgtggt gttcgcttcc agcgcagcgg tctacgggca gaacggcgaa ggcacggcga tcgacgaaga caccccgaag tcgccgctga cgccctacgc gtcggacaag ctggccagcg agtactacct cgatttctat cgccgccagc atgggctgga accggcgatc ttccgcttct tcaacgtcta cgggccacgc caggacccgt cctcgcccta ttccggggtg atcagcatat tcaccgagcg cgccctgaag gggacgccga tcaccgtgtt cggcgacggc gagcagaccc gcgacttcat ctacgtcgcc gacctggtcg atttcctggt ccaggcgctg gaggcgacgg cggtggaacc cggggcggtg aacgtcggcc tgaatcgcag catcagcctg aagcagttgc tggcagagat cggccaggta ctcggtggcc tgccgccggt gacccacgcc gacgcgcgag ccggtgacat caggcattcg cgggcgaaca acgagcgcct gctgcggcgt ttccgcttcg accagccgac gccgatgggc gtgggcctgg cgcgcctgct cggccgctga

Details for PsaeA.00085.a.B1.GE38197

HARVESTED ON:	6/11/2014
SEQUENCED ON:	6/12/2014
EXPECTED MW:	34kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMF ERPILITGGA GFIGSHLTDA LLARGYRVRV LDNLSTGKRD NLPLDDERVE LLEGDAADAA TLAAALKGCA AVAHLAAVAS VQASVDDPVA THQSNFIATL NLCEAMREQG VKRVVFASSA AVYGQNGEGT AIDEDTPKSP LTPYASDKLA SEYYLDFYRR QHGLEPAIFR FFNVYGPRQD PSSPYSGVIS IFTERALKGT PITVFGDGEQ TRDFIYVADL VDFLVQALEA TAVEPGAVNV GLNRSISLKQ LLAEIGQVLG GLPPVTHADA RAGDIRHSRA NNERLLRRFR FDQPTPMGVG LARLLGR

Validated NT Sequence

tactcactta gcggccgagc aggcgcgcca ggcccacgcc catcggcgtc ggctggtcga agcggaaacg ccgcagcagg cgctcgttgt tcgcccgcga atgcctgatg tcaccggctc gcgcgtcggc gtgggtcacc ggcggcaggc caccgagtac ctggccgatc tctgccagca actgcttcag gctgatgctg cgattcaggc cgacgttcac cgccccgggt tccaccgccg tcgcctccag cgcctggacc aggaaatcga ccaggtcggc gacgtagatg aagtcgcggg tctgctcgcc gtcgccgaac acggtgatcg gcgtcccctt cagggcgcgc tcggtgaata tgctgatcac cccggaatag ggcgaggacg ggtcctggcg tggcccgtag acgttgaaga agcggaagat cgccggttcc agcccatgct ggcggcgata gaaatcgagg tagtactcgc tggccagctt gtccgacgcg tagggcgtca gcggcgactt cggggtgtct tcgtcgatcg ccgtgccttc gccgttctgc ccgtagaccg ctgcgctgga agcgaacacc acgcgcttca cgccctgctc gcgcatcgct tcgcagaggt tcagggtggc gatgaaattg ctctggtggg tggcgaccgg gtcgtccacc gaggcctgta ccgaggcgac cgcggccagg tgggcgaccg ccgcgcagcc cttcagggcg gccgccaggg tcgccgcgtc ggcggcgtcg ccttcgagca gctctacccg ttcgtcgtcc aggggcaggt tgtcgcgctt gccggtggac aggttgtcca gcacacgcac gcggtagccg cgcgccagca gcgcgtcggt caggtgggag ccgatgaaac cggcgccgcc ggtgatcagg atggggcgtt caaacatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttg aacgccccat cctgatcacc ggcggcgccg gtttcatcgg ctcccacctg accgacgcgc tgctggcgcg cggctaccgc gtgcgtgtgc tggacaacct gtccaccggc aagcgcgaca acctgcccct ggacgacgaa cgggtagagc tgctcgaagg cgacgccgcc gacgcggcga ccctggcggc cgccctgaag ggctgcgcgg cggtcgccca cctggccgcg gtcgcctcgg tacaggcctc ggtggacgac ccggtcgcca cccaccagag caatttcatc gccaccctga acctctgcga agcgatgcgc gagcagggcg tgaagcgcgt ggtgttcgct tccagcgcag cggtctacgg gcagaacggc gaaggcacgg cgatcgacga agacaccccg aagtcgccgc tgacgcccta cgcgtcggac aagctggcca gcgagtacta cctcgatttc tatcgccgcc agcatgggct ggaaccggcg atcttccgct tcttcaacgt ctacgggcca cgccaggacc cgtcctcgcc ctattccggg gtgatcagca tattcaccga gcgcgccctg aaggggacgc cgatcaccgt gttcggcgac ggcgagcaga cccgcgactt catctacgtc gccgacctgg tcgatttcct ggtccaggcg ctggaggcga cggcggtgga acccggggcg gtgaacgtcg gcctgaatcg cagcatcagc ctgaagcagt tgctggcaga gatcggccag gtactcggtg gcctgccgcc ggtgacccac gccgacgcgc gagccggtga catcaggcat tcgcgggcga acaacgagcg cctgctgcgg cgtttccgct tcgaccagcc gacgccgatg ggcgtgggcc tggcgcgcct gctcggccgc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for PsaeA.00085.a.B1.PS02118

PURIFICATION DATe:	7/25/2014
CONCENTRATION:	38.56mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTTG AACGCCCCAT CCTGATCACC GGCGGCGCCG GTTTCATCGG CTCCCACCTG ACCGACGCGC TGCTGGCGCG CGGCTACCGC GTGCGTGTGC TGGACAACCT GTCCACCGGC AAGCGCGACA ACCTGCCCCT GGACGACGAA CGGGTAGAGC TGCTCGAAGG CGACGCCGCC GACGCGGCGA CCCTGGCGGC CGCCCTGAAG GGCTGCGCGG CGGTCGCCCA CCTGGCCGCG GTCGCCTCGG TACAGGCCTC GGTGGACGAC CCGGTCGCCA CCCACCAGAG CAATTTCATC GCCACCCTGA ACCTCTGCGA AGCGATGCGC GAGCAGGGCG TGAAGCGCGT GGTGTTCGCT TCCAGCGCAG CGGTCTACGG GCAGAACGGC GAAGGCACGG CGATCGACGA AGACACCCCG AAGTCGCCGC TGACGCCCTA CGCGTCGGAC AAGCTGGCCA GCGAGTACTA CCTCGATTTC TATCGCCGCC AGCATGGGCT GGAACCGGCG ATCTTCCGCT TCTTCAACGT CTACGGGCCA CGCCAGGACC CGTCCTCGCC CTATTCCGGG GTGATCAGCA TATTCACCGA GCGCGCCCTG AAGGGGACGC CGATCACCGT GTTCGGCGAC GGCGAGCAGA CCCGCGACTT CATCTACGTC GCCGACCTGG TCGATTTCCT GGTCCAGGCG CTGGAGGCGA CGGCGGTGGA ACCCGGGGCG GTGAACGTCG GCCTGAATCG CAGCATCAGC CTGAAGCAGT TGCTGGCAGA GATCGGCCAG GTACTCGGTG GCCTGCCGCC GGTGACCCAC GCCGACGCGC GAGCCGGTGA CATCAGGCAT TCGCGGGCGA ACAACGAGCG CCTGCTGCGG CGTTTCCGCT TCGACCAGCC GACGCCGATG GGCGTGGGCC TGGCGCGCCT GCTCGGCCGC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT

Details for PsaeA.00085.a.B1.PS02192

PURIFICATION DATe:	10/22/2014
CONCENTRATION:	44mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)