SSGCID: Target Page

PsaeA.00082.a

Porphobilinogen deaminase (PBG) (EC 2.5.1.61) (Hydroxymethylbilane synthase) (HMBS) (Pre-uroporphyrinogen synthase)

CENTER ID:	PsaeA.00082.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.00082.a.B1.GE38196	full length		1	313

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.5513
PGAT:	pseudomonas:PA5260
RefSeq:	NP_253947.1
UniProt:	Q60169

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSREIRIAT RQSALALWQA EYVKARLEQA HPGLTVTLLP MTSRGDKLLD APLAKIGGKG LFVKELETAL LEGAADIAVH SMKDVPMDFP EGLGLYTICE REDPRDAFVS NTYASLEQLP AGSVVGTSSL RRQAQLLARR PDLQIRFLRG NVNTRLAKLD AGEYDAIILA AAGLIRLGFE SRIRSSISVD DSLPAGGQGA VGIECRTADS DLHALLEPLH HTDTALRVTA ERALNKRLNG GCQVPIACYA IREGDQLWLR GLVGQPDGTQ LLRAEGRAPL AEAEALGVRV AEDLLEQGAE AILEAVYGEA GHP

NT Sequence

atgtcgtccc gcgaaatccg catcgccact cgccagagcg ccctcgcctt gtggcaggcc gaatacgtca aggcgcgcct ggaacaggcg catcccggct tgaccgtcac cctgctgccg atgaccagtc gcggcgacaa gctgctcgac gcaccgctgg cgaagatcgg cggcaagggc ctgttcgtca aggaactgga aaccgccctg ctcgaaggcg ccgccgacat cgcggtgcat tcgatgaagg acgtgccgat ggactttccc gaggggctcg gcctctacac catctgcgag cgcgaagacc cgcgtgacgc tttcgtctcg aacacctacg ccagcctcga gcaactgcca gccgggagcg tggtcggcac ctccagcctg cgccgccagg cgcagctgct ggcgcgccgg ccggacctgc agatccgttt cctgcggggc aacgtcaata cccgcctggc caagctggat gccggcgaat acgacgcgat catcctcgcc gccgccggcc tgatccgcct cggcttcgag tcgcgcatcc gctcctcgat cagtgtcgac gacagcctgc ccgccggcgg acaaggcgcc gtcggcatcg aatgccgtac ggccgacagc gacctgcatg ccttgctgga acccttgcac cataccgaca cggctctgcg ggtcactgcc gagcgcgcgc tgaacaagcg gctcaacggc ggttgccagg tgccgatcgc ctgctacgcc atccgcgagg gcgaccagct gtggctgcgc ggcctggtcg gccagcctga tggcacccag ttgctgcgcg ccgaggggcg ggcgccactg gcggaagccg aggcgctggg cgtgcgggtg gccgaggacc tgctggagca gggcgccgaa gcgattctcg aagccgtcta cggcgaggcc ggacatccgt ga

Details for PsaeA.00082.a.B1.GE38196

HARVESTED ON:	6/11/2014
SEQUENCED ON:	6/12/2014
EXPECTED MW:	35kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

tcttactcac ttacggatgt ccggcctcgc cgtagacggc ttcgagaatc gcttcggcgc cctgctccag caggtcctcg gccacccgca cgcccagcgc ctcggcttcc gccagtggcg cccgcccctc ggcgcgcagc aactgggtgc catcaggctg gccgaccagg ccgcgcagcc acagctggtc gccctcgcgg atggcgtagc aggcgatcgg cacctggcaa ccgccgttga gccgcttgtt cagcgcgcgc tcggcagtga cccgcagagc cgtgtcggta tggtgcaagg gttccagcaa ggcatgcagg tcgctgtcgg ccgtacggca ttcgatgccg acggcgcctt gtccgccggc gggcaggctg tcgtcgacac tgatcgagga gcggatgcgc gactcgaagc cgaggcggat caggccggcg gcggcgagga tgatcgcgtc gtattcgccg gcatccagct tggccaggcg ggtattgacg ttgccccgca ggaaacggat ctgcaggtcc ggccggcgcg ccagcagctg cgcctggcgg cgcaggctgg aggtgccgac cacgctcccg gctggcagtt gctcgaggct ggcgtaggtg ttcgagacga aagcgtcacg cgggtcttcg cgctcgcaga tggtgtagag gccgagcccc tcgggaaagt ccatcggcac gtccttcatc gaatgcaccg cgatgtcggc ggcgccttcg agcagggcgg tttccagttc cttgacgaac aggcccttgc cgccgatctt cgccagcggt gcgtcgagca gcttgtcgcc gcgactggtc atcggcagca gggtgacggt caagccggga tgcgcctgtt ccaggcgcgc cttgacgtat tcggcctgcc acaaggcgag ggcgctctgg cgagtggcga tgcggatttc gcgggacgac atatg

Expected Protein Sequence

MAHHHHHHMS SREIRIATRQ SALALWQAEY VKARLEQAHP GLTVTLLPMT SRGDKLLDAP LAKIGGKGLF VKELETALLE GAADIAVHSM KDVPMDFPEG LGLYTICERE DPRDAFVSNT YASLEQLPAG SVVGTSSLRR QAQLLARRPD LQIRFLRGNV NTRLAKLDAG EYDAIILAAA GLIRLGFESR IRSSISVDDS LPAGGQGAVG IECRTADSDL HALLEPLHHT DTALRVTAER ALNKRLNGGC QVPIACYAIR EGDQLWLRGL VGQPDGTQLL RAEGRAPLAE AEALGVRVAE DLLEQGAEAI LEAVYGEAGH P

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgt cccgcgaaat ccgcatcgcc actcgccaga gcgccctcgc cttgtggcag gccgaatacg tcaaggcgcg cctggaacag gcgcatcccg gcttgaccgt caccctgctg ccgatgacca gtcgcggcga caagctgctc gacgcaccgc tggcgaagat cggcggcaag ggcctgttcg tcaaggaact ggaaaccgcc ctgctcgaag gcgccgccga catcgcggtg cattcgatga aggacgtgcc gatggacttt cccgaggggc tcggcctcta caccatctgc gagcgcgaag acccgcgtga cgctttcgtc tcgaacacct acgccagcct cgagcaactg ccagccggga gcgtggtcgg cacctccagc ctgcgccgcc aggcgcagct gctggcgcgc cggccggacc tgcagatccg tttcctgcgg ggcaacgtca atacccgcct ggccaagctg gatgccggcg aatacgacgc gatcatcctc gccgccgccg gcctgatccg cctcggcttc gagtcgcgca tccgctcctc gatcagtgtc gacgacagcc tgcccgccgg cggacaaggc gccgtcggca tcgaatgccg tacggccgac agcgacctgc atgccttgct ggaacccttg caccataccg acacggctct gcgggtcact gccgagcgcg cgctgaacaa gcggctcaac ggcggttgcc aggtgccgat cgcctgctac gccatccgcg agggcgacca gctgtggctg cgcggcctgg tcggccagcc tgatggcacc cagttgctgc gcgccgaggg gcgggcgcca ctggcggaag ccgaggcgct gggcgtgcgg gtggccgagg acctgctgga gcagggcgcc gaagcgattc tcgaagccgt ctacggcgag gccggacatc cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat