PsaeA.00064.a

Adenylate kinase (AK) (EC 2.7.4.3) (ATP-AMP transphosphorylase) (ATP:AMP phosphotransferase) (Adenylate monophosphate kinase)

CENTER ID: PsaeA.00064.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00064.a.B1.GE38194 full length 1 215
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00064.a.B1.PW37556 full length 1 215

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.3855
PGAT: pseudomonas:PA3686
RefSeq: NP_252376.1
UniProt: Q9HXV4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVILLGAPG AGKGTQARFI TEKFGIPQIS TGDMLRAAVK AGSPLGQQVK GVMDSGGLVS DDIIIALIKE RITEADCAKG FLFDGFPRTI PQAEALKDAG VTIDHVVEIA VDDEEIVSRI AGRRVHPASG RVYHTEHNPP KVAGKDDVTG EELIQREDDK EETVRHRLSV YHSQTKPLVD FYQKLSAAEG TPKYHSIAGV GSVEQITAKV LSALS
NT Sequence
atgcgtgtga ttctgctcgg ggcacccggt gccggcaaag gcacccaggc acggttcatc accgagaagt tcggcattcc gcagatctcc accggcgaca tgctgcgcgc cgcagtcaag gccggcagcc cgctcggcca gcaggtgaaa ggcgtgatgg acagcggcgg cctggtatcc gacgacatca tcatcgccct gatcaaggaa cgcatcaccg aggccgattg cgccaagggc ttcctgttcg acggcttccc gcggaccatc ccgcaggccg aagcgctgaa ggacgccggc gtgaccatcg accatgtggt cgagatcgcc gtcgacgacg aggagatcgt gtcgcgtatc gccggtcgtc gcgtgcatcc ggcctccggc cgcgtgtacc acaccgagca caacccgccg aaggtcgccg gcaaggacga cgttaccggc gaagagctga tccagcgcga ggacgacaag gaagagaccg tgcgtcaccg cctgtcggtc taccattcgc agaccaagcc gctggtcgac ttctaccaga agctgtcggc cgccgaaggc accccgaaat accacagcat cgccggcgtc ggctcggtcg agcagatcac cgcgaaagtg ctttcggccc tgagctga
Details for PsaeA.00064.a.B1.GE38194
HARVESTED ON: 6/11/2014
SEQUENCED ON: 6/12/2014
EXPECTED MW: 24kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR VILLGAPGAG KGTQARFITE KFGIPQISTG DMLRAAVKAG SPLGQQVKGV MDSGGLVSDD IIIALIKERI TEADCAKGFL FDGFPRTIPQ AEALKDAGVT IDHVVEIAVD DEEIVSRIAG RRVHPASGRV YHTEHNPPKV AGKDDVTGEE LIQREDDKEE TVRHRLSVYH SQTKPLVDFY QKLSAAEGTP KYHSIAGVGS VEQITAKVLS ALS
Validated NT Sequence
atcttactca cttagctcag ggccgaaagc actttcgcgg tgatctgctc gaccgagccg acgccggcga tgctgtggta tttcggggtg ccttcggcgg ccgacagctt ctggtagaag tcgaccagcg gcttggtctg cgaatggtag accgacaggc ggtgacgcac ggtctcttcc ttgtcgtcct cgcgctggat cagctcttcg ccggtaacgt cgtccttgcc ggcgaccttc ggcgggttgt gctcggtgtg gtacacgcgg ccggaggccg gatgcacgcg acgaccggcg atacgcgaca cgatctcctc gtcgtcgacg gcgatctcga ccacatggtc gatggtcacg ccggcgtcct tcagcgcttc ggcctgcggg atggtccgcg ggaagccgtc gaacaggaag cccttggcgc aatcggcctc ggtgatgcgt tccttgatca gggcgatgat gatgtcgtcg gataccaggc cgccgctgtc catcacgcct ttcacctgct ggccgagcgg gctgccggcc ttgactgcgg cgcgcagcat gtcgccggtg gagatctgcg gaatgccgaa cttctcggtg atgaaccgtg cctgggtgcc tttgccggca ccgggtgccc cgagcagaat cacacgcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMR VILLGAPGAG KGTQARFITE KFGIPQISTG DMLRAAVKAG SPLGQQVKGV MDSGGLVSDD IIIALIKERI TEADCAKGFL FDGFPRTIPQ AEALKDAGVT IDHVVEIAVD DEEIVSRIAG RRVHPASGRV YHTEHNPPKV AGKDDVTGEE LIQREDDKEE TVRHRLSVYH SQTKPLVDFY QKLSAAEGTP KYHSIAGVGS VEQITAKVLS ALS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg tgattctgct cggggcaccc ggtgccggca aaggcaccca ggcacggttc atcaccgaga agttcggcat tccgcagatc tccaccggcg acatgctgcg cgccgcagtc aaggccggca gcccgctcgg ccagcaggtg aaaggcgtga tggacagcgg cggcctggta tccgacgaca tcatcatcgc cctgatcaag gaacgcatca ccgaggccga ttgcgccaag ggcttcctgt tcgacggctt cccgcggacc atcccgcagg ccgaagcgct gaaggacgcc ggcgtgacca tcgaccatgt ggtcgagatc gccgtcgacg acgaggagat cgtgtcgcgt atcgccggtc gtcgcgtgca tccggcctcc ggccgcgtgt accacaccga gcacaacccg ccgaaggtcg ccggcaagga cgacgttacc ggcgaagagc tgatccagcg cgaggacgac aaggaagaga ccgtgcgtca ccgcctgtcg gtctaccatt cgcagaccaa gccgctggtc gacttctacc agaagctgtc ggccgccgaa ggcaccccga aataccacag catcgccggc gtcggctcgg tcgagcagat caccgcgaaa gtgctttcgg ccctgagctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for PsaeA.00064.a.B1.PW37556
PURIFICATION DATe: 9/9/2014
CONCENTRATION: 25mg/ml
OBSERVED MW: 24kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR VILLGAPGAG KGTQARFITE KFGIPQISTG DMLRAAVKAG SPLGQQVKGV MDSGGLVSDD IIIALIKERI TEADCAKGFL FDGFPRTIPQ AEALKDAGVT IDHVVEIAVD DEEIVSRIAG RRVHPASGRV YHTEHNPPKV AGKDDVTGEE LIQREDDKEE TVRHRLSVYH SQTKPLVDFY QKLSAAEGTP KYHSIAGVGS VEQITAKVLS ALS
Validated NT Sequence
atcttactca cttagctcag ggccgaaagc actttcgcgg tgatctgctc gaccgagccg acgccggcga tgctgtggta tttcggggtg ccttcggcgg ccgacagctt ctggtagaag tcgaccagcg gcttggtctg cgaatggtag accgacaggc ggtgacgcac ggtctcttcc ttgtcgtcct cgcgctggat cagctcttcg ccggtaacgt cgtccttgcc ggcgaccttc ggcgggttgt gctcggtgtg gtacacgcgg ccggaggccg gatgcacgcg acgaccggcg atacgcgaca cgatctcctc gtcgtcgacg gcgatctcga ccacatggtc gatggtcacg ccggcgtcct tcagcgcttc ggcctgcggg atggtccgcg ggaagccgtc gaacaggaag cccttggcgc aatcggcctc ggtgatgcgt tccttgatca gggcgatgat gatgtcgtcg gataccaggc cgccgctgtc catcacgcct ttcacctgct ggccgagcgg gctgccggcc ttgactgcgg cgcgcagcat gtcgccggtg gagatctgcg gaatgccgaa cttctcggtg atgaaccgtg cctgggtgcc tttgccggca ccgggtgccc cgagcagaat cacacgcata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMR VILLGAPGAG KGTQARFITE KFGIPQISTG DMLRAAVKAG SPLGQQVKGV MDSGGLVSDD IIIALIKERI TEADCAKGFL FDGFPRTIPQ AEALKDAGVT IDHVVEIAVD DEEIVSRIAG RRVHPASGRV YHTEHNPPKV AGKDDVTGEE LIQREDDKEE TVRHRLSVYH SQTKPLVDFY QKLSAAEGTP KYHSIAGVGS VEQITAKVLS ALS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGTG TGATTCTGCT CGGGGCACCC GGTGCCGGCA AAGGCACCCA GGCACGGTTC ATCACCGAGA AGTTCGGCAT TCCGCAGATC TCCACCGGCG ACATGCTGCG CGCCGCAGTC AAGGCCGGCA GCCCGCTCGG CCAGCAGGTG AAAGGCGTGA TGGACAGCGG CGGCCTGGTA TCCGACGACA TCATCATCGC CCTGATCAAG GAACGCATCA CCGAGGCCGA TTGCGCCAAG GGCTTCCTGT TCGACGGCTT CCCGCGGACC ATCCCGCAGG CCGAAGCGCT GAAGGACGCC GGCGTGACCA TCGACCATGT GGTCGAGATC GCCGTCGACG ACGAGGAGAT CGTGTCGCGT ATCGCCGGTC GTCGCGTGCA TCCGGCCTCC GGCCGCGTGT ACCACACCGA GCACAACCCG CCGAAGGTCG CCGGCAAGGA CGACGTTACC GGCGAAGAGC TGATCCAGCG CGAGGACGAC AAGGAAGAGA CCGTGCGTCA CCGCCTGTCG GTCTACCATT CGCAGACCAA GCCGCTGGTC GACTTCTACC AGAAGCTGTC GGCCGCCGAA GGCACCCCGA AATACCACAG CATCGCCGGC GTCGGCTCGG TCGAGCAGAT CACCGCGAAA GTGCTTTCGG CCCTGAGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT