PsaeA.00063.a

Histidine--tRNA ligase (EC 6.1.1.21) (Histidyl-tRNA synthetase) (HisRS)

CENTER ID: PsaeA.00063.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00063.a.B1.GE38193 full length 1 429
PsaeA.00063.a.B3.GE40409 truncation 2 4 414
PsaeA.00063.a.B4.GE40410 truncation 3 4 429
PsaeA.00063.a.B5.GE40411 truncation 4 6 426
PsaeA.00063.a.B6.GE40412 truncation 5 6 406
PsaeA.00063.a.B7.GE40413 truncation 6 9 422
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00063.a.B3.PS38113 truncation 2 4 414
PsaeA.00063.a.B4.PS38114 truncation 3 4 429
PsaeA.00063.a.B6.PS38117 truncation 5 6 406
PsaeA.00063.a.B5.PS38120 truncation 4 6 426
PsaeA.00063.a.B1.PW37667 full length 1 429
PsaeA.00063.a.B7.PW37946 truncation 6 9 422

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.3981
PGAT: pseudomonas:PA3802
RefSeq: NP_252491.1
UniProt: Q9HXJ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKSLQAIRG MNDILPEQTP AWRYLERTFA GLLDGYGYSE IRLPILEFTE LFARGIGEGT DVVDKEMYTF LDRNGESLTM RPEGTAGCVR AVLEHGLSGG GQVQKLWYTG PMFRYEKPQK GRYRQFHQIG VEVFNLPGPD IDAELIILTW RLWQKLGMAD AVTLQLNTLG SSEARARYRE ALVAYLQERF EQLDEDSQRR MTTNPLRILD SKVESTQALL VGAPTLHDYL DEESIAHFEG LKARLDAVGL RYEINQKLVR GLDYYCRTAF EWVTDKLGAQ GTVCGGGRYD GLVSQFGGKP TPGVGFAMGV ERLVLLLETL GVIPAELNRP ADLYVCAFGE PAELAALTLA EQLRSAIPGI RLLVNAGAGS FKSQFKKADK SGARFALILG EDEVANRVVG FKPLRDEGEQ QSIAWDALPE HLAACLAQA
NT Sequence
gtgagcaagt ccctgcaagc cattcgtggc atgaacgaca tcctgccgga acagactccg gcctggcgct atctggaacg caccttcgcc ggcctgctgg acggttacgg ctacagcgag atccgcctgc ccatcctcga gttcaccgaa ctcttcgccc gcggcatcgg cgaaggcacc gatgtggtcg acaaggagat gtacaccttc ctcgaccgca acggcgaatc cctgaccatg cgtccggaag gtaccgccgg ttgcgtgcgc gccgtgctgg agcacggcct gagcggcggt ggccaggtgc agaagctctg gtacaccggg ccgatgttcc gttacgagaa accgcagaaa ggtcgctacc ggcagttcca ccagatcggc gtggaagtct tcaacctgcc cggtccggac atcgacgccg agttgatcat cctcacctgg cgcctgtggc agaagctcgg catggccgac gcggtgaccc tgcagctcaa caccctcggc tccagcgagg cccgcgcacg ctatcgcgag gccctggtgg cctatctgca ggagcgcttc gagcaactcg acgaagacag ccagcggcgc atgaccacca atccgctgcg aatcctcgac agcaaggtgg agagcaccca ggcgttgctg gttggcgcgc cgaccctgca cgactatctc gacgaagaat cgatcgccca cttcgagggt ctcaaggccc gcctggatgc tgtcggactt cgctacgaaa tcaaccagaa gctggtgcgc ggcctggact attactgccg cacggctttc gagtgggtca ccgacaagct tggcgcccag ggcaccgttt gcggcggtgg ccgctacgac gggctggtca gccagttcgg cggcaagccg accccgggtg tcggtttcgc catgggcgtg gaacgcctgg tgctgctgct ggaaaccttg ggggtgatcc ccgccgaact gaaccgtccg gccgacctct acgtctgcgc cttcggcgaa ccggccgagc tggccgccct gaccctggcc gaacagttgc gcagcgccat tcccggcata cgcctgctgg tcaacgccgg cgccggtagc ttcaagagcc agttcaagaa ggccgacaag agcggcgcgc gctttgccct gatcctcggg gaggacgagg tggccaatcg cgtggtaggt ttcaagcccc tgcgtgacga gggcgagcaa caaagcattg cctgggatgc tctgcccgag cacctggcag cctgtctggc acaggcttga
Details for PsaeA.00063.a.B1.GE38193
HARVESTED ON: 6/11/2014
SEQUENCED ON: 6/12/2014
EXPECTED MW: 49kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MNDILPEQTP AWRYLERTFA GLLDGYGYSE IRLPILEFTE LFARGIGEGT DVVDKEMYTF LDRNGESLTM RPEGTAGCVR AVLEHGLSGG GQVQKLWYTG PMFRYEKPQK GRYRQFHQIG VEVFNLPGPD IDAELIILTW RLWQKLGMAD AVTLQLNTLG SSEARARYRE ALVAYLQERF EQLDEDSQRR MTTNPLRILD SKVESTQALL VGAPTLHDYL DEESIAHFEG LKARLDAVGL RYEINQKLVR GLDYYCRTAF EWVTDKLGAQ GTVCGGGRYD GLVSQFGGKP TPGVGFAMGV ERLVLLLETL GVIPAELNRP ADLYVCAFGE PAELAALTLA EQLRSAIPGI RLLVNAGAGS FKSQFKKADK SGARFALILG EDEVANRVVG FKPLRDEGEQ QSIAWDALPE
Validated NT Sequence
gtgctcgggc agagcatccc aggcaatgct ttgttgctcg ccctcgtcac gcaggggctt gaaacctacc acgcgattgg ccacctcgtc ctccccgagg atcagggcaa agcgcgcgcc gctcttgtcg gccttcttga actggctctt gaagctaccg gcgccggcgt tgaccagcag gcgtatgccg ggaatggcgc tgcgcaactg ttcggccagg gtcagggcgg ccagctcggc cggttcgccg aaggcgcaga cgtagaggtc ggccggacgg ttcagttcgg cggggatcac ccccaaggtt tccagcagca gcaccaggcg ttccacgccc atggcgaaac cgacacccgg ggtcggcttg ccgccgaact ggctgaccag cccgtcgtag cggccaccgc cgcaaacggt gccctgggcg ccaagcttgt cggtgaccca ctcgaaagcc gtgcggcagt aatagtccag gccgcgcacc agcttctggt tgatttcgta gcgaagtccg acagcatcca ggcgggcctt gagaccctcg aagtgggcga tcgattcttc gtcgagatag tcgtgcaggg tcggcgcgcc aaccagcaac gcctgggtgc tctccacctt gctgtcgagg attcgcagcg gattggtggt catgcgccgc tggctgtctt cgtcgagttg ctcgaagcgc tcctgcagat aggccaccag ggcctcgcga tagcgtgcgc gggcctcgct ggagccgagg gtgttgagct gcagggtcac cgcgtcggcc atgccgagct tctgccacag gcgccaggtg aggatgatca actcggcgtc gatgtccgga ccgggcaggt tgaagacttc cacgccgatc tggtggaact gccggtagcg acctttctgc ggtttctcgt aacggaacat cggcccggtg taccagagct tctgcacctg gccaccgccg ctcaggccgt gctccagcac ggcgcgcacg caaccggcgg taccttccgg acgcatggtc agggattcgc cgttgcggtc gaggaaggtg tacatctcct tgtcgaccac atcggtgcct tcgccgatgc cgcgggcgaa gagttcggtg aactcgagga tgggcaggcg gatctcgctg tagccgtaac cgtccagcag gccggcgaag gtgcgttcca gatagcgcca ggccggagtc tgttccggca ggatgtcgtt catgccacga atggcttgca gggacttgct c
Expected Protein Sequence
MAHHHHHHMS KSLQAIRGMN DILPEQTPAW RYLERTFAGL LDGYGYSEIR LPILEFTELF ARGIGEGTDV VDKEMYTFLD RNGESLTMRP EGTAGCVRAV LEHGLSGGGQ VQKLWYTGPM FRYEKPQKGR YRQFHQIGVE VFNLPGPDID AELIILTWRL WQKLGMADAV TLQLNTLGSS EARARYREAL VAYLQERFEQ LDEDSQRRMT TNPLRILDSK VESTQALLVG APTLHDYLDE ESIAHFEGLK ARLDAVGLRY EINQKLVRGL DYYCRTAFEW VTDKLGAQGT VCGGGRYDGL VSQFGGKPTP GVGFAMGVER LVLLLETLGV IPAELNRPAD LYVCAFGEPA ELAALTLAEQ LRSAIPGIRL LVNAGAGSFK SQFKKADKSG ARFALILGED EVANRVVGFK PLRDEGEQQS IAWDALPEHL AACLAQA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagca agtccctgca agccattcgt ggcatgaacg acatcctgcc ggaacagact ccggcctggc gctatctgga acgcaccttc gccggcctgc tggacggtta cggctacagc gagatccgcc tgcccatcct cgagttcacc gaactcttcg cccgcggcat cggcgaaggc accgatgtgg tcgacaagga gatgtacacc ttcctcgacc gcaacggcga atccctgacc atgcgtccgg aaggtaccgc cggttgcgtg cgcgccgtgc tggagcacgg cctgagcggc ggtggccagg tgcagaagct ctggtacacc gggccgatgt tccgttacga gaaaccgcag aaaggtcgct accggcagtt ccaccagatc ggcgtggaag tcttcaacct gcccggtccg gacatcgacg ccgagttgat catcctcacc tggcgcctgt ggcagaagct cggcatggcc gacgcggtga ccctgcagct caacaccctc ggctccagcg aggcccgcgc acgctatcgc gaggccctgg tggcctatct gcaggagcgc ttcgagcaac tcgacgaaga cagccagcgg cgcatgacca ccaatccgct gcgaatcctc gacagcaagg tggagagcac ccaggcgttg ctggttggcg cgccgaccct gcacgactat ctcgacgaag aatcgatcgc ccacttcgag ggtctcaagg cccgcctgga tgctgtcgga cttcgctacg aaatcaacca gaagctggtg cgcggcctgg actattactg ccgcacggct ttcgagtggg tcaccgacaa gcttggcgcc cagggcaccg tttgcggcgg tggccgctac gacgggctgg tcagccagtt cggcggcaag ccgaccccgg gtgtcggttt cgccatgggc gtggaacgcc tggtgctgct gctggaaacc ttgggggtga tccccgccga actgaaccgt ccggccgacc tctacgtctg cgccttcggc gaaccggccg agctggccgc cctgaccctg gccgaacagt tgcgcagcgc cattcccggc atacgcctgc tggtcaacgc cggcgccggt agcttcaaga gccagttcaa gaaggccgac aagagcggcg cgcgctttgc cctgatcctc ggggaggacg aggtggccaa tcgcgtggta ggtttcaagc ccctgcgtga cgagggcgag caacaaagca ttgcctggga tgctctgccc gagcacctgg cagcctgtct ggcacaggct tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for PsaeA.00063.a.B3.GE40409
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHSL QAIRGMNDIL PEQTPAWRYL ERTFAGLLDG YGYSEIRLPI LEFTELFARG IGEGTDVVDK EMYTFLDRNG ESLTMRPEGT AGCVRAVLEH GLSGGGQVQK LWYTGPMFRY EKPQKGRYRQ FHQIGVEVFN LPGPDIDAEL IILTWRLWQK LGMADAVTLQ LNTLGSSEAR ARYREALVAY LQERFEQLDE DSQRRMTTNP LRILDSKVES TQALLVGAPT LHDYLDEESI AHFEGLKARL DAVGLRYEIN QKLVRGLDYY CRTAFEWVTD KLGAQGTVCG GGRYDGLVSQ FGGKPTPGVG FAMGVERLVL LLETLGVIPA ELNRPADLYV CAFGEPAELA ALTLAEQLRS AIPGIRLLVN AGAGSFKSQF KKADKSGARF ALILGEDEVA NRVVGFKPLR DEGEQQSIA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccctgc aagccattcg tggcatgaac gacatcctgc cggaacagac tccggcctgg cgctatctgg aacgcacctt cgccggcctg ctggacggtt acggctacag cgagatccgc ctgcccatcc tcgagttcac cgaactcttc gcccgcggca tcggcgaagg caccgatgtg gtcgacaagg agatgtacac cttcctcgac cgcaacggcg aatccctgac catgcgtccg gaaggtaccg ccggttgcgt gcgcgccgtg ctggagcacg gcctgagcgg cggtggccag gtgcagaagc tctggtacac cgggccgatg ttccgttacg agaaaccgca gaaaggtcgc taccggcagt tccaccagat cggcgtggaa gtcttcaacc tgcccggtcc ggacatcgac gccgagttga tcatcctcac ctggcgcctg tggcagaagc tcggcatggc cgacgcggtg accctgcagc tcaacaccct cggctccagc gaggcccgcg cacgctatcg cgaggccctg gtggcctatc tgcaggagcg cttcgagcaa ctcgacgaag acagccagcg gcgcatgacc accaatccgc tgcgaatcct cgacagcaag gtggagagca cccaggcgtt gctggttggc gcgccgaccc tgcacgacta tctcgacgaa gaatcgatcg cccacttcga gggtctcaag gcccgcctgg atgctgtcgg acttcgctac gaaatcaacc agaagctggt gcgcggcctg gactattact gccgcacggc tttcgagtgg gtcaccgaca agcttggcgc ccagggcacc gtttgcggcg gtggccgcta cgacgggctg gtcagccagt tcggcggcaa gccgaccccg ggtgtcggtt tcgccatggg cgtggaacgc ctggtgctgc tgctggaaac cttgggggtg atccccgccg aactgaaccg tccggccgac ctctacgtct gcgccttcgg cgaaccggcc gagctggccg ccctgaccct ggccgaacag ttgcgcagcg ccattcccgg catacgcctg ctggtcaacg ccggcgccgg tagcttcaag agccagttca agaaggccga caagagcggc gcgcgctttg ccctgatcct cggggaggac gaggtggcca atcgcgtggt aggtttcaag cccctgcgtg acgagggcga gcaacaaagc attgcctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for PsaeA.00063.a.B4.GE40410
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHSL QAIRGMNDIL PEQTPAWRYL ERTFAGLLDG YGYSEIRLPI LEFTELFARG IGEGTDVVDK EMYTFLDRNG ESLTMRPEGT AGCVRAVLEH GLSGGGQVQK LWYTGPMFRY EKPQKGRYRQ FHQIGVEVFN LPGPDIDAEL IILTWRLWQK LGMADAVTLQ LNTLGSSEAR ARYREALVAY LQERFEQLDE DSQRRMTTNP LRILDSKVES TQALLVGAPT LHDYLDEESI AHFEGLKARL DAVGLRYEIN QKLVRGLDYY CRTAFEWVTD KLGAQGTVCG GGRYDGLVSQ FGGKPTPGVG FAMGVERLVL LLETLGVIPA ELNRPADLYV CAFGEPAELA ALTLAEQLRS AIPGIRLLVN AGAGSFKSQF KKADKSGARF ALILGEDEVA NRVVGFKPLR DEGEQQSIAW DALPEHLAAC LAQA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccctgc aagccattcg tggcatgaac gacatcctgc cggaacagac tccggcctgg cgctatctgg aacgcacctt cgccggcctg ctggacggtt acggctacag cgagatccgc ctgcccatcc tcgagttcac cgaactcttc gcccgcggca tcggcgaagg caccgatgtg gtcgacaagg agatgtacac cttcctcgac cgcaacggcg aatccctgac catgcgtccg gaaggtaccg ccggttgcgt gcgcgccgtg ctggagcacg gcctgagcgg cggtggccag gtgcagaagc tctggtacac cgggccgatg ttccgttacg agaaaccgca gaaaggtcgc taccggcagt tccaccagat cggcgtggaa gtcttcaacc tgcccggtcc ggacatcgac gccgagttga tcatcctcac ctggcgcctg tggcagaagc tcggcatggc cgacgcggtg accctgcagc tcaacaccct cggctccagc gaggcccgcg cacgctatcg cgaggccctg gtggcctatc tgcaggagcg cttcgagcaa ctcgacgaag acagccagcg gcgcatgacc accaatccgc tgcgaatcct cgacagcaag gtggagagca cccaggcgtt gctggttggc gcgccgaccc tgcacgacta tctcgacgaa gaatcgatcg cccacttcga gggtctcaag gcccgcctgg atgctgtcgg acttcgctac gaaatcaacc agaagctggt gcgcggcctg gactattact gccgcacggc tttcgagtgg gtcaccgaca agcttggcgc ccagggcacc gtttgcggcg gtggccgcta cgacgggctg gtcagccagt tcggcggcaa gccgaccccg ggtgtcggtt tcgccatggg cgtggaacgc ctggtgctgc tgctggaaac cttgggggtg atccccgccg aactgaaccg tccggccgac ctctacgtct gcgccttcgg cgaaccggcc gagctggccg ccctgaccct ggccgaacag ttgcgcagcg ccattcccgg catacgcctg ctggtcaacg ccggcgccgg tagcttcaag agccagttca agaaggccga caagagcggc gcgcgctttg ccctgatcct cggggaggac gaggtggcca atcgcgtggt aggtttcaag cccctgcgtg acgagggcga gcaacaaagc attgcctggg atgctctgcc cgagcacctg gcagcctgtc tggcacaggc ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for PsaeA.00063.a.B5.GE40411
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHQA IRGMNDILPE QTPAWRYLER TFAGLLDGYG YSEIRLPILE FTELFARGIG EGTDVVDKEM YTFLDRNGES LTMRPEGTAG CVRAVLEHGL SGGGQVQKLW YTGPMFRYEK PQKGRYRQFH QIGVEVFNLP GPDIDAELII LTWRLWQKLG MADAVTLQLN TLGSSEARAR YREALVAYLQ ERFEQLDEDS QRRMTTNPLR ILDSKVESTQ ALLVGAPTLH DYLDEESIAH FEGLKARLDA VGLRYEINQK LVRGLDYYCR TAFEWVTDKL GAQGTVCGGG RYDGLVSQFG GKPTPGVGFA MGVERLVLLL ETLGVIPAEL NRPADLYVCA FGEPAELAAL TLAEQLRSAI PGIRLLVNAG AGSFKSQFKK ADKSGARFAL ILGEDEVANR VVGFKPLRDE GEQQSIAWDA LPEHLAACL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaagcca ttcgtggcat gaacgacatc ctgccggaac agactccggc ctggcgctat ctggaacgca ccttcgccgg cctgctggac ggttacggct acagcgagat ccgcctgccc atcctcgagt tcaccgaact cttcgcccgc ggcatcggcg aaggcaccga tgtggtcgac aaggagatgt acaccttcct cgaccgcaac ggcgaatccc tgaccatgcg tccggaaggt accgccggtt gcgtgcgcgc cgtgctggag cacggcctga gcggcggtgg ccaggtgcag aagctctggt acaccgggcc gatgttccgt tacgagaaac cgcagaaagg tcgctaccgg cagttccacc agatcggcgt ggaagtcttc aacctgcccg gtccggacat cgacgccgag ttgatcatcc tcacctggcg cctgtggcag aagctcggca tggccgacgc ggtgaccctg cagctcaaca ccctcggctc cagcgaggcc cgcgcacgct atcgcgaggc cctggtggcc tatctgcagg agcgcttcga gcaactcgac gaagacagcc agcggcgcat gaccaccaat ccgctgcgaa tcctcgacag caaggtggag agcacccagg cgttgctggt tggcgcgccg accctgcacg actatctcga cgaagaatcg atcgcccact tcgagggtct caaggcccgc ctggatgctg tcggacttcg ctacgaaatc aaccagaagc tggtgcgcgg cctggactat tactgccgca cggctttcga gtgggtcacc gacaagcttg gcgcccaggg caccgtttgc ggcggtggcc gctacgacgg gctggtcagc cagttcggcg gcaagccgac cccgggtgtc ggtttcgcca tgggcgtgga acgcctggtg ctgctgctgg aaaccttggg ggtgatcccc gccgaactga accgtccggc cgacctctac gtctgcgcct tcggcgaacc ggccgagctg gccgccctga ccctggccga acagttgcgc agcgccattc ccggcatacg cctgctggtc aacgccggcg ccggtagctt caagagccag ttcaagaagg ccgacaagag cggcgcgcgc tttgccctga tcctcgggga ggacgaggtg gccaatcgcg tggtaggttt caagcccctg cgtgacgagg gcgagcaaca aagcattgcc tgggatgctc tgcccgagca cctggcagcc tgtctgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for PsaeA.00063.a.B6.GE40412
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHQA IRGMNDILPE QTPAWRYLER TFAGLLDGYG YSEIRLPILE FTELFARGIG EGTDVVDKEM YTFLDRNGES LTMRPEGTAG CVRAVLEHGL SGGGQVQKLW YTGPMFRYEK PQKGRYRQFH QIGVEVFNLP GPDIDAELII LTWRLWQKLG MADAVTLQLN TLGSSEARAR YREALVAYLQ ERFEQLDEDS QRRMTTNPLR ILDSKVESTQ ALLVGAPTLH DYLDEESIAH FEGLKARLDA VGLRYEINQK LVRGLDYYCR TAFEWVTDKL GAQGTVCGGG RYDGLVSQFG GKPTPGVGFA MGVERLVLLL ETLGVIPAEL NRPADLYVCA FGEPAELAAL TLAEQLRSAI PGIRLLVNAG AGSFKSQFKK ADKSGARFAL ILGEDEVANR VVGFKPLRD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaagcca ttcgtggcat gaacgacatc ctgccggaac agactccggc ctggcgctat ctggaacgca ccttcgccgg cctgctggac ggttacggct acagcgagat ccgcctgccc atcctcgagt tcaccgaact cttcgcccgc ggcatcggcg aaggcaccga tgtggtcgac aaggagatgt acaccttcct cgaccgcaac ggcgaatccc tgaccatgcg tccggaaggt accgccggtt gcgtgcgcgc cgtgctggag cacggcctga gcggcggtgg ccaggtgcag aagctctggt acaccgggcc gatgttccgt tacgagaaac cgcagaaagg tcgctaccgg cagttccacc agatcggcgt ggaagtcttc aacctgcccg gtccggacat cgacgccgag ttgatcatcc tcacctggcg cctgtggcag aagctcggca tggccgacgc ggtgaccctg cagctcaaca ccctcggctc cagcgaggcc cgcgcacgct atcgcgaggc cctggtggcc tatctgcagg agcgcttcga gcaactcgac gaagacagcc agcggcgcat gaccaccaat ccgctgcgaa tcctcgacag caaggtggag agcacccagg cgttgctggt tggcgcgccg accctgcacg actatctcga cgaagaatcg atcgcccact tcgagggtct caaggcccgc ctggatgctg tcggacttcg ctacgaaatc aaccagaagc tggtgcgcgg cctggactat tactgccgca cggctttcga gtgggtcacc gacaagcttg gcgcccaggg caccgtttgc ggcggtggcc gctacgacgg gctggtcagc cagttcggcg gcaagccgac cccgggtgtc ggtttcgcca tgggcgtgga acgcctggtg ctgctgctgg aaaccttggg ggtgatcccc gccgaactga accgtccggc cgacctctac gtctgcgcct tcggcgaacc ggccgagctg gccgccctga ccctggccga acagttgcgc agcgccattc ccggcatacg cctgctggtc aacgccggcg ccggtagctt caagagccag ttcaagaagg ccgacaagag cggcgcgcgc tttgccctga tcctcgggga ggacgaggtg gccaatcgcg tggtaggttt caagcccctg cgtgactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for PsaeA.00063.a.B7.GE40413
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/19/2016
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHRG MNDILPEQTP AWRYLERTFA GLLDGYGYSE IRLPILEFTE LFARGIGEGT DVVDKEMYTF LDRNGESLTM RPEGTAGCVR AVLEHGLSGG GQVQKLWYTG PMFRYEKPQK GRYRQFHQIG VEVFNLPGPD IDAELIILTW RLWQKLGMAD AVTLQLNTLG SSEARARYRE ALVAYLQERF EQLDEDSQRR MTTNPLRILD SKVESTQALL VGAPTLHDYL DEESIAHFEG LKARLDAVGL RYEINQKLVR GLDYYCRTAF EWVTDKLGAQ GTVCGGGRYD GLVSQFGGKP TPGVGFAMGV ERLVLLLETL GVIPAELNRP ADLYVCAFGE PAELAALTLA EQLRSAIPGI RLLVNAGAGS FKSQFKKADK SGARFALILG EDEVANRVVG FKPLRDEGEQ QSIAWDALPE HL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgtggca tgaacgacat cctgccggaa cagactccgg cctggcgcta tctggaacgc accttcgccg gcctgctgga cggttacggc tacagcgaga tccgcctgcc catcctcgag ttcaccgaac tcttcgcccg cggcatcggc gaaggcaccg atgtggtcga caaggagatg tacaccttcc tcgaccgcaa cggcgaatcc ctgaccatgc gtccggaagg taccgccggt tgcgtgcgcg ccgtgctgga gcacggcctg agcggcggtg gccaggtgca gaagctctgg tacaccgggc cgatgttccg ttacgagaaa ccgcagaaag gtcgctaccg gcagttccac cagatcggcg tggaagtctt caacctgccc ggtccggaca tcgacgccga gttgatcatc ctcacctggc gcctgtggca gaagctcggc atggccgacg cggtgaccct gcagctcaac accctcggct ccagcgaggc ccgcgcacgc tatcgcgagg ccctggtggc ctatctgcag gagcgcttcg agcaactcga cgaagacagc cagcggcgca tgaccaccaa tccgctgcga atcctcgaca gcaaggtgga gagcacccag gcgttgctgg ttggcgcgcc gaccctgcac gactatctcg acgaagaatc gatcgcccac ttcgagggtc tcaaggcccg cctggatgct gtcggacttc gctacgaaat caaccagaag ctggtgcgcg gcctggacta ttactgccgc acggctttcg agtgggtcac cgacaagctt ggcgcccagg gcaccgtttg cggcggtggc cgctacgacg ggctggtcag ccagttcggc ggcaagccga ccccgggtgt cggtttcgcc atgggcgtgg aacgcctggt gctgctgctg gaaaccttgg gggtgatccc cgccgaactg aaccgtccgg ccgacctcta cgtctgcgcc ttcggcgaac cggccgagct ggccgccctg accctggccg aacagttgcg cagcgccatt cccggcatac gcctgctggt caacgccggc gccggtagct tcaagagcca gttcaagaag gccgacaaga gcggcgcgcg ctttgccctg atcctcgggg aggacgaggt ggccaatcgc gtggtaggtt tcaagcccct gcgtgacgag ggcgagcaac aaagcattgc ctgggatgct ctgcccgagc acctgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for PsaeA.00063.a.B3.PS38113
PURIFICATION DATe: 11/10/2016
CONCENTRATION: 30.3mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHSL QAIRGMNDIL PEQTPAWRYL ERTFAGLLDG YGYSEIRLPI LEFTELFARG IGEGTDVVDK EMYTFLDRNG ESLTMRPEGT AGCVRAVLEH GLSGGGQVQK LWYTGPMFRY EKPQKGRYRQ FHQIGVEVFN LPGPDIDAEL IILTWRLWQK LGMADAVTLQ LNTLGSSEAR ARYREALVAY LQERFEQLDE DSQRRMTTNP LRILDSKVES TQALLVGAPT LHDYLDEESI AHFEGLKARL DAVGLRYEIN QKLVRGLDYY CRTAFEWVTD KLGAQGTVCG GGRYDGLVSQ FGGKPTPGVG FAMGVERLVL LLETLGVIPA ELNRPADLYV CAFGEPAELA ALTLAEQLRS AIPGIRLLVN AGAGSFKSQF KKADKSGARF ALILGEDEVA NRVVGFKPLR DEGEQQSIA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTCCCTGC AAGCCATTCG TGGCATGAAC GACATCCTGC CGGAACAGAC TCCGGCCTGG CGCTATCTGG AACGCACCTT CGCCGGCCTG CTGGACGGTT ACGGCTACAG CGAGATCCGC CTGCCCATCC TCGAGTTCAC CGAACTCTTC GCCCGCGGCA TCGGCGAAGG CACCGATGTG GTCGACAAGG AGATGTACAC CTTCCTCGAC CGCAACGGCG AATCCCTGAC CATGCGTCCG GAAGGTACCG CCGGTTGCGT GCGCGCCGTG CTGGAGCACG GCCTGAGCGG CGGTGGCCAG GTGCAGAAGC TCTGGTACAC CGGGCCGATG TTCCGTTACG AGAAACCGCA GAAAGGTCGC TACCGGCAGT TCCACCAGAT CGGCGTGGAA GTCTTCAACC TGCCCGGTCC GGACATCGAC GCCGAGTTGA TCATCCTCAC CTGGCGCCTG TGGCAGAAGC TCGGCATGGC CGACGCGGTG ACCCTGCAGC TCAACACCCT CGGCTCCAGC GAGGCCCGCG CACGCTATCG CGAGGCCCTG GTGGCCTATC TGCAGGAGCG CTTCGAGCAA CTCGACGAAG ACAGCCAGCG GCGCATGACC ACCAATCCGC TGCGAATCCT CGACAGCAAG GTGGAGAGCA CCCAGGCGTT GCTGGTTGGC GCGCCGACCC TGCACGACTA TCTCGACGAA GAATCGATCG CCCACTTCGA GGGTCTCAAG GCCCGCCTGG ATGCTGTCGG ACTTCGCTAC GAAATCAACC AGAAGCTGGT GCGCGGCCTG GACTATTACT GCCGCACGGC TTTCGAGTGG GTCACCGACA AGCTTGGCGC CCAGGGCACC GTTTGCGGCG GTGGCCGCTA CGACGGGCTG GTCAGCCAGT TCGGCGGCAA GCCGACCCCG GGTGTCGGTT TCGCCATGGG CGTGGAACGC CTGGTGCTGC TGCTGGAAAC CTTGGGGGTG ATCCCCGCCG AACTGAACCG TCCGGCCGAC CTCTACGTCT GCGCCTTCGG CGAACCGGCC GAGCTGGCCG CCCTGACCCT GGCCGAACAG TTGCGCAGCG CCATTCCCGG CATACGCCTG CTGGTCAACG CCGGCGCCGG TAGCTTCAAG AGCCAGTTCA AGAAGGCCGA CAAGAGCGGC GCGCGCTTTG CCCTGATCCT CGGGGAGGAC GAGGTGGCCA ATCGCGTGGT AGGTTTCAAG CCCCTGCGTG ACGAGGGCGA GCAACAAAGC ATTGCCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for PsaeA.00063.a.B4.PS38114
PURIFICATION DATe: 11/9/2016
CONCENTRATION: 61.09mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHSL QAIRGMNDIL PEQTPAWRYL ERTFAGLLDG YGYSEIRLPI LEFTELFARG IGEGTDVVDK EMYTFLDRNG ESLTMRPEGT AGCVRAVLEH GLSGGGQVQK LWYTGPMFRY EKPQKGRYRQ FHQIGVEVFN LPGPDIDAEL IILTWRLWQK LGMADAVTLQ LNTLGSSEAR ARYREALVAY LQERFEQLDE DSQRRMTTNP LRILDSKVES TQALLVGAPT LHDYLDEESI AHFEGLKARL DAVGLRYEIN QKLVRGLDYY CRTAFEWVTD KLGAQGTVCG GGRYDGLVSQ FGGKPTPGVG FAMGVERLVL LLETLGVIPA ELNRPADLYV CAFGEPAELA ALTLAEQLRS AIPGIRLLVN AGAGSFKSQF KKADKSGARF ALILGEDEVA NRVVGFKPLR DEGEQQSIAW DALPEHLAAC LAQA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTCCCTGC AAGCCATTCG TGGCATGAAC GACATCCTGC CGGAACAGAC TCCGGCCTGG CGCTATCTGG AACGCACCTT CGCCGGCCTG CTGGACGGTT ACGGCTACAG CGAGATCCGC CTGCCCATCC TCGAGTTCAC CGAACTCTTC GCCCGCGGCA TCGGCGAAGG CACCGATGTG GTCGACAAGG AGATGTACAC CTTCCTCGAC CGCAACGGCG AATCCCTGAC CATGCGTCCG GAAGGTACCG CCGGTTGCGT GCGCGCCGTG CTGGAGCACG GCCTGAGCGG CGGTGGCCAG GTGCAGAAGC TCTGGTACAC CGGGCCGATG TTCCGTTACG AGAAACCGCA GAAAGGTCGC TACCGGCAGT TCCACCAGAT CGGCGTGGAA GTCTTCAACC TGCCCGGTCC GGACATCGAC GCCGAGTTGA TCATCCTCAC CTGGCGCCTG TGGCAGAAGC TCGGCATGGC CGACGCGGTG ACCCTGCAGC TCAACACCCT CGGCTCCAGC GAGGCCCGCG CACGCTATCG CGAGGCCCTG GTGGCCTATC TGCAGGAGCG CTTCGAGCAA CTCGACGAAG ACAGCCAGCG GCGCATGACC ACCAATCCGC TGCGAATCCT CGACAGCAAG GTGGAGAGCA CCCAGGCGTT GCTGGTTGGC GCGCCGACCC TGCACGACTA TCTCGACGAA GAATCGATCG CCCACTTCGA GGGTCTCAAG GCCCGCCTGG ATGCTGTCGG ACTTCGCTAC GAAATCAACC AGAAGCTGGT GCGCGGCCTG GACTATTACT GCCGCACGGC TTTCGAGTGG GTCACCGACA AGCTTGGCGC CCAGGGCACC GTTTGCGGCG GTGGCCGCTA CGACGGGCTG GTCAGCCAGT TCGGCGGCAA GCCGACCCCG GGTGTCGGTT TCGCCATGGG CGTGGAACGC CTGGTGCTGC TGCTGGAAAC CTTGGGGGTG ATCCCCGCCG AACTGAACCG TCCGGCCGAC CTCTACGTCT GCGCCTTCGG CGAACCGGCC GAGCTGGCCG CCCTGACCCT GGCCGAACAG TTGCGCAGCG CCATTCCCGG CATACGCCTG CTGGTCAACG CCGGCGCCGG TAGCTTCAAG AGCCAGTTCA AGAAGGCCGA CAAGAGCGGC GCGCGCTTTG CCCTGATCCT CGGGGAGGAC GAGGTGGCCA ATCGCGTGGT AGGTTTCAAG CCCCTGCGTG ACGAGGGCGA GCAACAAAGC ATTGCCTGGG ATGCTCTGCC CGAGCACCTG GCAGCCTGTC TGGCACAGGC TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT
Details for PsaeA.00063.a.B6.PS38117
PURIFICATION DATe: 11/15/2016
CONCENTRATION: 38.3mg/ml
OBSERVED MW: 46kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHQA IRGMNDILPE QTPAWRYLER TFAGLLDGYG YSEIRLPILE FTELFARGIG EGTDVVDKEM YTFLDRNGES LTMRPEGTAG CVRAVLEHGL SGGGQVQKLW YTGPMFRYEK PQKGRYRQFH QIGVEVFNLP GPDIDAELII LTWRLWQKLG MADAVTLQLN TLGSSEARAR YREALVAYLQ ERFEQLDEDS QRRMTTNPLR ILDSKVESTQ ALLVGAPTLH DYLDEESIAH FEGLKARLDA VGLRYEINQK LVRGLDYYCR TAFEWVTDKL GAQGTVCGGG RYDGLVSQFG GKPTPGVGFA MGVERLVLLL ETLGVIPAEL NRPADLYVCA FGEPAELAAL TLAEQLRSAI PGIRLLVNAG AGSFKSQFKK ADKSGARFAL ILGEDEVANR VVGFKPLRD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCAAGCCA TTCGTGGCAT GAACGACATC CTGCCGGAAC AGACTCCGGC CTGGCGCTAT CTGGAACGCA CCTTCGCCGG CCTGCTGGAC GGTTACGGCT ACAGCGAGAT CCGCCTGCCC ATCCTCGAGT TCACCGAACT CTTCGCCCGC GGCATCGGCG AAGGCACCGA TGTGGTCGAC AAGGAGATGT ACACCTTCCT CGACCGCAAC GGCGAATCCC TGACCATGCG TCCGGAAGGT ACCGCCGGTT GCGTGCGCGC CGTGCTGGAG CACGGCCTGA GCGGCGGTGG CCAGGTGCAG AAGCTCTGGT ACACCGGGCC GATGTTCCGT TACGAGAAAC CGCAGAAAGG TCGCTACCGG CAGTTCCACC AGATCGGCGT GGAAGTCTTC AACCTGCCCG GTCCGGACAT CGACGCCGAG TTGATCATCC TCACCTGGCG CCTGTGGCAG AAGCTCGGCA TGGCCGACGC GGTGACCCTG CAGCTCAACA CCCTCGGCTC CAGCGAGGCC CGCGCACGCT ATCGCGAGGC CCTGGTGGCC TATCTGCAGG AGCGCTTCGA GCAACTCGAC GAAGACAGCC AGCGGCGCAT GACCACCAAT CCGCTGCGAA TCCTCGACAG CAAGGTGGAG AGCACCCAGG CGTTGCTGGT TGGCGCGCCG ACCCTGCACG ACTATCTCGA CGAAGAATCG ATCGCCCACT TCGAGGGTCT CAAGGCCCGC CTGGATGCTG TCGGACTTCG CTACGAAATC AACCAGAAGC TGGTGCGCGG CCTGGACTAT TACTGCCGCA CGGCTTTCGA GTGGGTCACC GACAAGCTTG GCGCCCAGGG CACCGTTTGC GGCGGTGGCC GCTACGACGG GCTGGTCAGC CAGTTCGGCG GCAAGCCGAC CCCGGGTGTC GGTTTCGCCA TGGGCGTGGA ACGCCTGGTG CTGCTGCTGG AAACCTTGGG GGTGATCCCC GCCGAACTGA ACCGTCCGGC CGACCTCTAC GTCTGCGCCT TCGGCGAACC GGCCGAGCTG GCCGCCCTGA CCCTGGCCGA ACAGTTGCGC AGCGCCATTC CCGGCATACG CCTGCTGGTC AACGCCGGCG CCGGTAGCTT CAAGAGCCAG TTCAAGAAGG CCGACAAGAG CGGCGCGCGC TTTGCCCTGA TCCTCGGGGA GGACGAGGTG GCCAATCGCG TGGTAGGTTT CAAGCCCCTG CGTGACTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for PsaeA.00063.a.B5.PS38120
PURIFICATION DATe: 11/15/2016
CONCENTRATION: 23.9mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHQA IRGMNDILPE QTPAWRYLER TFAGLLDGYG YSEIRLPILE FTELFARGIG EGTDVVDKEM YTFLDRNGES LTMRPEGTAG CVRAVLEHGL SGGGQVQKLW YTGPMFRYEK PQKGRYRQFH QIGVEVFNLP GPDIDAELII LTWRLWQKLG MADAVTLQLN TLGSSEARAR YREALVAYLQ ERFEQLDEDS QRRMTTNPLR ILDSKVESTQ ALLVGAPTLH DYLDEESIAH FEGLKARLDA VGLRYEINQK LVRGLDYYCR TAFEWVTDKL GAQGTVCGGG RYDGLVSQFG GKPTPGVGFA MGVERLVLLL ETLGVIPAEL NRPADLYVCA FGEPAELAAL TLAEQLRSAI PGIRLLVNAG AGSFKSQFKK ADKSGARFAL ILGEDEVANR VVGFKPLRDE GEQQSIAWDA LPEHLAACL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCAAGCCA TTCGTGGCAT GAACGACATC CTGCCGGAAC AGACTCCGGC CTGGCGCTAT CTGGAACGCA CCTTCGCCGG CCTGCTGGAC GGTTACGGCT ACAGCGAGAT CCGCCTGCCC ATCCTCGAGT TCACCGAACT CTTCGCCCGC GGCATCGGCG AAGGCACCGA TGTGGTCGAC AAGGAGATGT ACACCTTCCT CGACCGCAAC GGCGAATCCC TGACCATGCG TCCGGAAGGT ACCGCCGGTT GCGTGCGCGC CGTGCTGGAG CACGGCCTGA GCGGCGGTGG CCAGGTGCAG AAGCTCTGGT ACACCGGGCC GATGTTCCGT TACGAGAAAC CGCAGAAAGG TCGCTACCGG CAGTTCCACC AGATCGGCGT GGAAGTCTTC AACCTGCCCG GTCCGGACAT CGACGCCGAG TTGATCATCC TCACCTGGCG CCTGTGGCAG AAGCTCGGCA TGGCCGACGC GGTGACCCTG CAGCTCAACA CCCTCGGCTC CAGCGAGGCC CGCGCACGCT ATCGCGAGGC CCTGGTGGCC TATCTGCAGG AGCGCTTCGA GCAACTCGAC GAAGACAGCC AGCGGCGCAT GACCACCAAT CCGCTGCGAA TCCTCGACAG CAAGGTGGAG AGCACCCAGG CGTTGCTGGT TGGCGCGCCG ACCCTGCACG ACTATCTCGA CGAAGAATCG ATCGCCCACT TCGAGGGTCT CAAGGCCCGC CTGGATGCTG TCGGACTTCG CTACGAAATC AACCAGAAGC TGGTGCGCGG CCTGGACTAT TACTGCCGCA CGGCTTTCGA GTGGGTCACC GACAAGCTTG GCGCCCAGGG CACCGTTTGC GGCGGTGGCC GCTACGACGG GCTGGTCAGC CAGTTCGGCG GCAAGCCGAC CCCGGGTGTC GGTTTCGCCA TGGGCGTGGA ACGCCTGGTG CTGCTGCTGG AAACCTTGGG GGTGATCCCC GCCGAACTGA ACCGTCCGGC CGACCTCTAC GTCTGCGCCT TCGGCGAACC GGCCGAGCTG GCCGCCCTGA CCCTGGCCGA ACAGTTGCGC AGCGCCATTC CCGGCATACG CCTGCTGGTC AACGCCGGCG CCGGTAGCTT CAAGAGCCAG TTCAAGAAGG CCGACAAGAG CGGCGCGCGC TTTGCCCTGA TCCTCGGGGA GGACGAGGTG GCCAATCGCG TGGTAGGTTT CAAGCCCCTG CGTGACGAGG GCGAGCAACA AAGCATTGCC TGGGATGCTC TGCCCGAGCA CCTGGCAGCC TGTCTGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for PsaeA.00063.a.B1.PW37667
PURIFICATION DATe: 3/9/2015
CONCENTRATION: 12mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MNDILPEQTP AWRYLERTFA GLLDGYGYSE IRLPILEFTE LFARGIGEGT DVVDKEMYTF LDRNGESLTM RPEGTAGCVR AVLEHGLSGG GQVQKLWYTG PMFRYEKPQK GRYRQFHQIG VEVFNLPGPD IDAELIILTW RLWQKLGMAD AVTLQLNTLG SSEARARYRE ALVAYLQERF EQLDEDSQRR MTTNPLRILD SKVESTQALL VGAPTLHDYL DEESIAHFEG LKARLDAVGL RYEINQKLVR GLDYYCRTAF EWVTDKLGAQ GTVCGGGRYD GLVSQFGGKP TPGVGFAMGV ERLVLLLETL GVIPAELNRP ADLYVCAFGE PAELAALTLA EQLRSAIPGI RLLVNAGAGS FKSQFKKADK SGARFALILG EDEVANRVVG FKPLRDEGEQ QSIAWDALPE
Validated NT Sequence
gtgctcgggc agagcatccc aggcaatgct ttgttgctcg ccctcgtcac gcaggggctt gaaacctacc acgcgattgg ccacctcgtc ctccccgagg atcagggcaa agcgcgcgcc gctcttgtcg gccttcttga actggctctt gaagctaccg gcgccggcgt tgaccagcag gcgtatgccg ggaatggcgc tgcgcaactg ttcggccagg gtcagggcgg ccagctcggc cggttcgccg aaggcgcaga cgtagaggtc ggccggacgg ttcagttcgg cggggatcac ccccaaggtt tccagcagca gcaccaggcg ttccacgccc atggcgaaac cgacacccgg ggtcggcttg ccgccgaact ggctgaccag cccgtcgtag cggccaccgc cgcaaacggt gccctgggcg ccaagcttgt cggtgaccca ctcgaaagcc gtgcggcagt aatagtccag gccgcgcacc agcttctggt tgatttcgta gcgaagtccg acagcatcca ggcgggcctt gagaccctcg aagtgggcga tcgattcttc gtcgagatag tcgtgcaggg tcggcgcgcc aaccagcaac gcctgggtgc tctccacctt gctgtcgagg attcgcagcg gattggtggt catgcgccgc tggctgtctt cgtcgagttg ctcgaagcgc tcctgcagat aggccaccag ggcctcgcga tagcgtgcgc gggcctcgct ggagccgagg gtgttgagct gcagggtcac cgcgtcggcc atgccgagct tctgccacag gcgccaggtg aggatgatca actcggcgtc gatgtccgga ccgggcaggt tgaagacttc cacgccgatc tggtggaact gccggtagcg acctttctgc ggtttctcgt aacggaacat cggcccggtg taccagagct tctgcacctg gccaccgccg ctcaggccgt gctccagcac ggcgcgcacg caaccggcgg taccttccgg acgcatggtc agggattcgc cgttgcggtc gaggaaggtg tacatctcct tgtcgaccac atcggtgcct tcgccgatgc cgcgggcgaa gagttcggtg aactcgagga tgggcaggcg gatctcgctg tagccgtaac cgtccagcag gccggcgaag gtgcgttcca gatagcgcca ggccggagtc tgttccggca ggatgtcgtt catgccacga atggcttgca gggacttgct c
Expressed Protein Sequence
MAHHHHHHMS KSLQAIRGMN DILPEQTPAW RYLERTFAGL LDGYGYSEIR LPILEFTELF ARGIGEGTDV VDKEMYTFLD RNGESLTMRP EGTAGCVRAV LEHGLSGGGQ VQKLWYTGPM FRYEKPQKGR YRQFHQIGVE VFNLPGPDID AELIILTWRL WQKLGMADAV TLQLNTLGSS EARARYREAL VAYLQERFEQ LDEDSQRRMT TNPLRILDSK VESTQALLVG APTLHDYLDE ESIAHFEGLK ARLDAVGLRY EINQKLVRGL DYYCRTAFEW VTDKLGAQGT VCGGGRYDGL VSQFGGKPTP GVGFAMGVER LVLLLETLGV IPAELNRPAD LYVCAFGEPA ELAALTLAEQ LRSAIPGIRL LVNAGAGSFK SQFKKADKSG ARFALILGED EVANRVVGFK PLRDEGEQQS IAWDALPEHL AACLAQA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGAGCA AGTCCCTGCA AGCCATTCGT GGCATGAACG ACATCCTGCC GGAACAGACT CCGGCCTGGC GCTATCTGGA ACGCACCTTC GCCGGCCTGC TGGACGGTTA CGGCTACAGC GAGATCCGCC TGCCCATCCT CGAGTTCACC GAACTCTTCG CCCGCGGCAT CGGCGAAGGC ACCGATGTGG TCGACAAGGA GATGTACACC TTCCTCGACC GCAACGGCGA ATCCCTGACC ATGCGTCCGG AAGGTACCGC CGGTTGCGTG CGCGCCGTGC TGGAGCACGG CCTGAGCGGC GGTGGCCAGG TGCAGAAGCT CTGGTACACC GGGCCGATGT TCCGTTACGA GAAACCGCAG AAAGGTCGCT ACCGGCAGTT CCACCAGATC GGCGTGGAAG TCTTCAACCT GCCCGGTCCG GACATCGACG CCGAGTTGAT CATCCTCACC TGGCGCCTGT GGCAGAAGCT CGGCATGGCC GACGCGGTGA CCCTGCAGCT CAACACCCTC GGCTCCAGCG AGGCCCGCGC ACGCTATCGC GAGGCCCTGG TGGCCTATCT GCAGGAGCGC TTCGAGCAAC TCGACGAAGA CAGCCAGCGG CGCATGACCA CCAATCCGCT GCGAATCCTC GACAGCAAGG TGGAGAGCAC CCAGGCGTTG CTGGTTGGCG CGCCGACCCT GCACGACTAT CTCGACGAAG AATCGATCGC CCACTTCGAG GGTCTCAAGG CCCGCCTGGA TGCTGTCGGA CTTCGCTACG AAATCAACCA GAAGCTGGTG CGCGGCCTGG ACTATTACTG CCGCACGGCT TTCGAGTGGG TCACCGACAA GCTTGGCGCC CAGGGCACCG TTTGCGGCGG TGGCCGCTAC GACGGGCTGG TCAGCCAGTT CGGCGGCAAG CCGACCCCGG GTGTCGGTTT CGCCATGGGC GTGGAACGCC TGGTGCTGCT GCTGGAAACC TTGGGGGTGA TCCCCGCCGA ACTGAACCGT CCGGCCGACC TCTACGTCTG CGCCTTCGGC GAACCGGCCG AGCTGGCCGC CCTGACCCTG GCCGAACAGT TGCGCAGCGC CATTCCCGGC ATACGCCTGC TGGTCAACGC CGGCGCCGGT AGCTTCAAGA GCCAGTTCAA GAAGGCCGAC AAGAGCGGCG CGCGCTTTGC CCTGATCCTC GGGGAGGACG AGGTGGCCAA TCGCGTGGTA GGTTTCAAGC CCCTGCGTGA CGAGGGCGAG CAACAAAGCA TTGCCTGGGA TGCTCTGCCC GAGCACCTGG CAGCCTGTCT GGCACAGGCT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for PsaeA.00063.a.B7.PW37946
PURIFICATION DATe: 10/17/2016
CONCENTRATION: 24.25mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHRG MNDILPEQTP AWRYLERTFA GLLDGYGYSE IRLPILEFTE LFARGIGEGT DVVDKEMYTF LDRNGESLTM RPEGTAGCVR AVLEHGLSGG GQVQKLWYTG PMFRYEKPQK GRYRQFHQIG VEVFNLPGPD IDAELIILTW RLWQKLGMAD AVTLQLNTLG SSEARARYRE ALVAYLQERF EQLDEDSQRR MTTNPLRILD SKVESTQALL VGAPTLHDYL DEESIAHFEG LKARLDAVGL RYEINQKLVR GLDYYCRTAF EWVTDKLGAQ GTVCGGGRYD GLVSQFGGKP TPGVGFAMGV ERLVLLLETL GVIPAELNRP ADLYVCAFGE PAELAALTLA EQLRSAIPGI RLLVNAGAGS FKSQFKKADK SGARFALILG EDEVANRVVG FKPLRDEGEQ QSIAWDALPE HL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCGTGGCA TGAACGACAT CCTGCCGGAA CAGACTCCGG CCTGGCGCTA TCTGGAACGC ACCTTCGCCG GCCTGCTGGA CGGTTACGGC TACAGCGAGA TCCGCCTGCC CATCCTCGAG TTCACCGAAC TCTTCGCCCG CGGCATCGGC GAAGGCACCG ATGTGGTCGA CAAGGAGATG TACACCTTCC TCGACCGCAA CGGCGAATCC CTGACCATGC GTCCGGAAGG TACCGCCGGT TGCGTGCGCG CCGTGCTGGA GCACGGCCTG AGCGGCGGTG GCCAGGTGCA GAAGCTCTGG TACACCGGGC CGATGTTCCG TTACGAGAAA CCGCAGAAAG GTCGCTACCG GCAGTTCCAC CAGATCGGCG TGGAAGTCTT CAACCTGCCC GGTCCGGACA TCGACGCCGA GTTGATCATC CTCACCTGGC GCCTGTGGCA GAAGCTCGGC ATGGCCGACG CGGTGACCCT GCAGCTCAAC ACCCTCGGCT CCAGCGAGGC CCGCGCACGC TATCGCGAGG CCCTGGTGGC CTATCTGCAG GAGCGCTTCG AGCAACTCGA CGAAGACAGC CAGCGGCGCA TGACCACCAA TCCGCTGCGA ATCCTCGACA GCAAGGTGGA GAGCACCCAG GCGTTGCTGG TTGGCGCGCC GACCCTGCAC GACTATCTCG ACGAAGAATC GATCGCCCAC TTCGAGGGTC TCAAGGCCCG CCTGGATGCT GTCGGACTTC GCTACGAAAT CAACCAGAAG CTGGTGCGCG GCCTGGACTA TTACTGCCGC ACGGCTTTCG AGTGGGTCAC CGACAAGCTT GGCGCCCAGG GCACCGTTTG CGGCGGTGGC CGCTACGACG GGCTGGTCAG CCAGTTCGGC GGCAAGCCGA CCCCGGGTGT CGGTTTCGCC ATGGGCGTGG AACGCCTGGT GCTGCTGCTG GAAACCTTGG GGGTGATCCC CGCCGAACTG AACCGTCCGG CCGACCTCTA CGTCTGCGCC TTCGGCGAAC CGGCCGAGCT GGCCGCCCTG ACCCTGGCCG AACAGTTGCG CAGCGCCATT CCCGGCATAC GCCTGCTGGT CAACGCCGGC GCCGGTAGCT TCAAGAGCCA GTTCAAGAAG GCCGACAAGA GCGGCGCGCG CTTTGCCCTG ATCCTCGGGG AGGACGAGGT GGCCAATCGC GTGGTAGGTT TCAAGCCCCT GCGTGACGAG GGCGAGCAAC AAAGCATTGC CTGGGATGCT CTGCCCGAGC ACCTGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT