PsaeA.00035.a

Ribose-phosphate pyrophosphokinase (RPPK) (EC 2.7.6.1) (Phosphoribosyl pyrophosphate synthase) (P-Rib-PP synthase) (PRPP synthase)

CENTER ID: PsaeA.00035.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.00035.a.B1.GE38190 full length 1 313
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4892
PGAT: pseudomonas:PA4670
RefSeq: NP_253359.1
UniProt: Q9HVC5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKMMVFTGN ANPDLARRVV RQLHIPLGDV SVGKFSDGEI SVEINENVRG KDVFLIQPTC APTNDNLMEL VVMADAFRRS SATRITAVIP YFGYARQDRR PRSARVAISA KVVADMLTVV GVNRVLTVDL HADQIQGFFD IPVDNIYGSP VLVDDIEDQR FENLMIVSPD IGGVVRARAV AKSLGVDLAI IDKRRPKANQ SEVMHIIGDV EGRTCVLVDD MVDTAGTLGH AAKALKEHGA AKVIAYCTHP VLSGRAIENI EKSVLDELVV TNTIPLSAAA QACGRIRQLD IAPVVAEAMR RISNEESISA MFR
NT Sequence
gtgtccaaga tgatggtctt cacggggaac gctaaccccg atcttgcacg acgcgtcgtg cgtcagctgc acattcctct tggcgatgtt tcagttggca aattctccga tggcgaaatc agcgtcgaaa tcaacgaaaa cgtccgcggc aaggacgtat tcctgattca gccgacctgc gcgccgacca acgacaacct gatggaactg gtggtgatgg ccgatgcctt ccgccgctcc tcggccactc gtatcaccgc tgtcatcccc tacttcggct atgcccgcca ggatcgccgt ccgcgttccg cccgcgtggc catcagcgcc aaggtagtgg ccgacatgct gaccgtcgtc ggggtcaacc gtgtcctcac ggtcgacctg cacgcggatc agatccaggg cttcttcgat atcccggtag acaacatcta cggttcgccc gtgctggtgg acgatatcga agaccagcgc ttcgagaacc tgatgatcgt ctccccggac atcggcggcg tggtgcgcgc gcgcgccgtg gccaagtccc tgggcgtgga tcttgcgatc atcgacaagc gtcgtcccaa ggccaaccag tccgaagtga tgcacatcat cggtgacgtc gaaggccgta cctgcgtact ggtagacgac atggtcgata ccgccggcac cctcggtcac gccgccaagg ccctgaaaga gcacggcgcc gccaaggtca tcgcctactg cacccacccg gtgctgtccg gccgtgccat cgagaacatc gagaaatccg tactggacga gctggtggtg accaacacca tcccgctgtc cgccgctgcc caggcctgtg gccgcatccg ccagctggac atcgctccgg tcgttgcgga agccatgcgc cggatcagca acgaagaatc gatcagcgcg atgttccgct ga
Details for PsaeA.00035.a.B1.GE38190
HARVESTED ON: 6/11/2014
SEQUENCED ON: 6/12/2014
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV SKMMVFTGNA NPDLARRVVR QLHIPLGDVS VGKFSDGEIS VEINENVRGK DVFLIQPTCA PTNDNLMELV VMADAFRRSS ATRITAVIPY FGYARQDRRP RSARVAISAK VVADMLTVVG VNRVLTVDLH ADQIQGFFDI PVDNIYGSPV LVDDIEDQRF ENLMIVSPDI GGVVRARAVA KSLGVDLAII DKRRPKANQS EVMHIIGDVE GRTCVLVDDM VDTAGTLGHA AKALKEHGAA KVIAYCTHPV LSGRAIENIE KSVLDELVVT NTIPLSAAAQ ACGRIRQLDI APVVAEAMRR ISNEESISAM FR
Validated NT Sequence
atcttactca cttagcggaa catcgcgctg atcgattctt cgttgctgat ccggcgcatg gcttccgcaa cgaccggagc gatgtccagc tggcggatgc ggccacaggc ctgggcagcg gcggacagcg ggatggtgtt ggtcaccacc agctcgtcca gtacggattt ctcgatgttc tcgatggcac ggccggacag caccgggtgg gtgcagtagg cgatgacctt ggcggcgccg tgctctttca gggccttggc ggcgtgaccg agggtgccgg cggtatcgac catgtcgtct accagtacgc aggtacggcc ttcgacgtca ccgatgatgt gcatcacttc ggactggttg gccttgggac gacgcttgtc gatgatcgca agatccacgc ccagggactt ggccacggcg cgcgcgcgca ccacgccgcc gatgtccggg gagacgatca tcaggttctc gaagcgctgg tcttcgatat cgtccaccag cacgggcgaa ccgtagatgt tgtctaccgg gatatcgaag aagccctgga tctgatccgc gtgcaggtcg accgtgagga cacggttgac cccgacgacg gtcagcatgt cggccactac cttggcgctg atggccacgc gggcggaacg cggacggcga tcctggcggg catagccgaa gtaggggatg acagcggtga tacgagtggc cgaggagcgg cggaaggcat cggccatcac caccagttcc atcaggttgt cgttggtcgg cgcgcaggtc ggctgaatca ggaatacgtc cttgccgcgg acgttttcgt tgatttcgac gctgatttcg ccatcggaga atttgccaac tgaaacatcg ccaagaggaa tgtgcagctg acgcacgacg cgtcgtgcaa gatcggggtt agcgttcccc gtgaagacca tcatcttgga caccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMS KMMVFTGNAN PDLARRVVRQ LHIPLGDVSV GKFSDGEISV EINENVRGKD VFLIQPTCAP TNDNLMELVV MADAFRRSSA TRITAVIPYF GYARQDRRPR SARVAISAKV VADMLTVVGV NRVLTVDLHA DQIQGFFDIP VDNIYGSPVL VDDIEDQRFE NLMIVSPDIG GVVRARAVAK SLGVDLAIID KRRPKANQSE VMHIIGDVEG RTCVLVDDMV DTAGTLGHAA KALKEHGAAK VIAYCTHPVL SGRAIENIEK SVLDELVVTN TIPLSAAAQA CGRIRQLDIA PVVAEAMRRI SNEESISAMF R
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtcca agatgatggt cttcacgggg aacgctaacc ccgatcttgc acgacgcgtc gtgcgtcagc tgcacattcc tcttggcgat gtttcagttg gcaaattctc cgatggcgaa atcagcgtcg aaatcaacga aaacgtccgc ggcaaggacg tattcctgat tcagccgacc tgcgcgccga ccaacgacaa cctgatggaa ctggtggtga tggccgatgc cttccgccgc tcctcggcca ctcgtatcac cgctgtcatc ccctacttcg gctatgcccg ccaggatcgc cgtccgcgtt ccgcccgcgt ggccatcagc gccaaggtag tggccgacat gctgaccgtc gtcggggtca accgtgtcct cacggtcgac ctgcacgcgg atcagatcca gggcttcttc gatatcccgg tagacaacat ctacggttcg cccgtgctgg tggacgatat cgaagaccag cgcttcgaga acctgatgat cgtctccccg gacatcggcg gcgtggtgcg cgcgcgcgcc gtggccaagt ccctgggcgt ggatcttgcg atcatcgaca agcgtcgtcc caaggccaac cagtccgaag tgatgcacat catcggtgac gtcgaaggcc gtacctgcgt actggtagac gacatggtcg ataccgccgg caccctcggt cacgccgcca aggccctgaa agagcacggc gccgccaagg tcatcgccta ctgcacccac ccggtgctgt ccggccgtgc catcgagaac atcgagaaat ccgtactgga cgagctggtg gtgaccaaca ccatcccgct gtccgccgct gcccaggcct gtggccgcat ccgccagctg gacatcgctc cggtcgttgc ggaagccatg cgccggatca gcaacgaaga atcgatcagc gcgatgttcc gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat