PsaeA.00025.a
NH(3)-dependent NAD(+) synthetase (EC 6.3.1.5)
| CENTER ID: | PsaeA.00025.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00025.a.B1.GE38189 | full length | 1 | 275 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00025.a.B1.PW37534 | full length | 1 | 275 | ||
| PsaeA.00025.a.B1.PW37555 | full length | 1 | 275 |
Structures
4XFD
DEPOSITED: 12/26/2014
DETERMINATION: XRay
CLONE: PsaeA.00025.a.B1.GE38189
PROTEIN: PsaeA.00025.a.B1.PW37555
5F23
DEPOSITED: 12/1/2015
DETERMINATION: XRay
CLONE: PsaeA.00025.a.B1.GE38189
PROTEIN: PsaeA.00025.a.B1.PW37555
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.5153 |
| PGAT: | pseudomonas:PA4920 |
| RefSeq: | NP_253607.1 |
| UniProt: | Q9HUP3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQQIQRDIAQ ALQVQPPFQS EADVQAQIAR RIAFIQQCLK DSGLKTLVLG ISGGVDSLTA GLLAQRAVEQ LREQTGDQAY RFIAVRLPYQ VQQDEADAQA SLATIRADEE QTVNIGPSVK ALAEQLEALE GLEPAKSDFV IGNIKARIRM VAQYAIAGAR GGLVIGTDHA AEAVMGFFTK FGDGACDLAP LSGLAKHQVR ALARALGAPE NLVEKIPTAD LEDLRPGHPD EASHGVTYAE IDAFLHGQPL REEAARVIVD TYHKTQHKRE LPKAP
NT Sequence
atgcaacaga tccaacgcga catcgcccaa gccctgcagg tccagccgcc gttccagtcg gaggccgacg tgcaggcgca gatcgcccgg cgcatcgcct tcatccagca gtgcctgaag gattccgggc tgaagaccct ggtgctgggg atcagcggcg gcgtcgactc gctcaccgcc ggcctgctgg cccagcgcgc cgtcgagcaa ctgcgcgagc agaccggcga ccaggcctac cgcttcatcg ccgtgcgcct gccctaccag gtgcagcagg acgaggccga tgcccaggcc tcgctggcga ccatccgcgc cgacgaagag cagaccgtca acatcggtcc gtcggtgaaa gccctggcgg aacagctgga agccctggaa ggactcgagc cggcgaagag cgacttcgtc atcggcaata tcaaggcgcg catccgcatg gtcgcccagt acgccatcgc cggcgcccgc ggcggcctgg tgatcggcac cgaccatgct gccgaggcgg tcatggggtt cttcaccaag ttcggcgacg gcgcctgcga cctggctccg ctcagcggcc tggccaagca tcaggtacgc gccctcgccc gcgccctcgg cgctccggag aacctggtgg agaagatccc caccgccgac ctcgaggacc tgcgccccgg ccatccggac gaggcttccc acggcgtcac ctatgccgag atcgacgcct tcctgcacgg ccagccgctg cgcgaggaag ctgcgcgggt gatcgtcgac acctaccaca agacccagca caagcgcgaa ctgccgaagg cgccctga
Details for PsaeA.00025.a.B1.GE38189
| HARVESTED ON: | 6/11/2014 |
| SEQUENCED ON: | 6/12/2014 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMQ QIQRDIAQAL QVQPPFQSEA DVQAQIARRI AFIQQCLKDS GLKTLVLGIS GGVDSLTAGL LAQRAVEQLR EQTGDQAYRF IAVRLPYQVQ QDEADAQASL ATIRADEEQT VNIGPSVKAL AEQLEALEGL EPAKSDFVIG NIKARIRMVA QYAIAGARGG LVIGTDHAAE AVMGFFTKFG DGACDLAPLS GLAKHQVRAL ARALGAPENL VEKIPTADLE DLRPGHPDEA SHGVTYAEID AFLHGQPLRE EAARVIVDTY HKTQHKRELP KAP
Validated NT Sequence
tactcactta gggcgccttc ggcagttcgc gcttgtgctg ggtcttgtgg taggtgtcga cgatcacccg cgcagcttcc tcgcgcagcg gctggccgtg caggaaggcg tcgatctcgg cataggtgac gccgtgggaa gcctcgtccg gatggccggg gcgcaggtcc tcgaggtcgg cggtggggat cttctccacc aggttctccg gagcgccgag ggcgcgggcg agggcgcgta cctgatgctt ggccaggccg ctgagcggag ccaggtcgca ggcgccgtcg ccgaacttgg tgaagaaccc catgaccgcc tcggcagcat ggtcggtgcc gatcaccagg ccgccgcggg cgccggcgat ggcgtactgg gcgaccatgc ggatgcgcgc cttgatattg ccgatgacga agtcgctctt cgccggctcg agtccttcca gggcttccag ctgttccgcc agggctttca ccgacggacc gatgttgacg gtctgctctt cgtcggcgcg gatggtcgcc agcgaggcct gggcatcggc ctcgtcctgc tgcacctggt agggcaggcg cacggcgatg aagcggtagg cctggtcgcc ggtctgctcg cgcagttgct cgacggcgcg ctgggccagc aggccggcgg tgagcgagtc gacgccgccg ctgatcccca gcaccagggt cttcagcccg gaatccttca ggcactgctg gatgaaggcg atgcgccggg cgatctgcgc ctgcacgtcg gcctccgact ggaacggcgg ctggacctgc agggcttggg cgatgtcgcg ttggatctgt tgcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMQ QIQRDIAQAL QVQPPFQSEA DVQAQIARRI AFIQQCLKDS GLKTLVLGIS GGVDSLTAGL LAQRAVEQLR EQTGDQAYRF IAVRLPYQVQ QDEADAQASL ATIRADEEQT VNIGPSVKAL AEQLEALEGL EPAKSDFVIG NIKARIRMVA QYAIAGARGG LVIGTDHAAE AVMGFFTKFG DGACDLAPLS GLAKHQVRAL ARALGAPENL VEKIPTADLE DLRPGHPDEA SHGVTYAEID AFLHGQPLRE EAARVIVDTY HKTQHKRELP KAP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac agatccaacg cgacatcgcc caagccctgc aggtccagcc gccgttccag tcggaggccg acgtgcaggc gcagatcgcc cggcgcatcg ccttcatcca gcagtgcctg aaggattccg ggctgaagac cctggtgctg gggatcagcg gcggcgtcga ctcgctcacc gccggcctgc tggcccagcg cgccgtcgag caactgcgcg agcagaccgg cgaccaggcc taccgcttca tcgccgtgcg cctgccctac caggtgcagc aggacgaggc cgatgcccag gcctcgctgg cgaccatccg cgccgacgaa gagcagaccg tcaacatcgg tccgtcggtg aaagccctgg cggaacagct ggaagccctg gaaggactcg agccggcgaa gagcgacttc gtcatcggca atatcaaggc gcgcatccgc atggtcgccc agtacgccat cgccggcgcc cgcggcggcc tggtgatcgg caccgaccat gctgccgagg cggtcatggg gttcttcacc aagttcggcg acggcgcctg cgacctggct ccgctcagcg gcctggccaa gcatcaggta cgcgccctcg cccgcgccct cggcgctccg gagaacctgg tggagaagat ccccaccgcc gacctcgagg acctgcgccc cggccatccg gacgaggctt cccacggcgt cacctatgcc gagatcgacg ccttcctgca cggccagccg ctgcgcgagg aagctgcgcg ggtgatcgtc gacacctacc acaagaccca gcacaagcgc gaactgccga aggcgccctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for PsaeA.00025.a.B1.PW37534
| PURIFICATION DATe: | 8/4/2014 |
| CONCENTRATION: | 17.7mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ QIQRDIAQAL QVQPPFQSEA DVQAQIARRI AFIQQCLKDS GLKTLVLGIS GGVDSLTAGL LAQRAVEQLR EQTGDQAYRF IAVRLPYQVQ QDEADAQASL ATIRADEEQT VNIGPSVKAL AEQLEALEGL EPAKSDFVIG NIKARIRMVA QYAIAGARGG LVIGTDHAAE AVMGFFTKFG DGACDLAPLS GLAKHQVRAL ARALGAPENL VEKIPTADLE DLRPGHPDEA SHGVTYAEID AFLHGQPLRE EAARVIVDTY HKTQHKRELP KAP
Validated NT Sequence
tactcactta gggcgccttc ggcagttcgc gcttgtgctg ggtcttgtgg taggtgtcga cgatcacccg cgcagcttcc tcgcgcagcg gctggccgtg caggaaggcg tcgatctcgg cataggtgac gccgtgggaa gcctcgtccg gatggccggg gcgcaggtcc tcgaggtcgg cggtggggat cttctccacc aggttctccg gagcgccgag ggcgcgggcg agggcgcgta cctgatgctt ggccaggccg ctgagcggag ccaggtcgca ggcgccgtcg ccgaacttgg tgaagaaccc catgaccgcc tcggcagcat ggtcggtgcc gatcaccagg ccgccgcggg cgccggcgat ggcgtactgg gcgaccatgc ggatgcgcgc cttgatattg ccgatgacga agtcgctctt cgccggctcg agtccttcca gggcttccag ctgttccgcc agggctttca ccgacggacc gatgttgacg gtctgctctt cgtcggcgcg gatggtcgcc agcgaggcct gggcatcggc ctcgtcctgc tgcacctggt agggcaggcg cacggcgatg aagcggtagg cctggtcgcc ggtctgctcg cgcagttgct cgacggcgcg ctgggccagc aggccggcgg tgagcgagtc gacgccgccg ctgatcccca gcaccagggt cttcagcccg gaatccttca ggcactgctg gatgaaggcg atgcgccggg cgatctgcgc ctgcacgtcg gcctccgact ggaacggcgg ctggacctgc agggcttggg cgatgtcgcg ttggatctgt tgcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMQ QIQRDIAQAL QVQPPFQSEA DVQAQIARRI AFIQQCLKDS GLKTLVLGIS GGVDSLTAGL LAQRAVEQLR EQTGDQAYRF IAVRLPYQVQ QDEADAQASL ATIRADEEQT VNIGPSVKAL AEQLEALEGL EPAKSDFVIG NIKARIRMVA QYAIAGARGG LVIGTDHAAE AVMGFFTKFG DGACDLAPLS GLAKHQVRAL ARALGAPENL VEKIPTADLE DLRPGHPDEA SHGVTYAEID AFLHGQPLRE EAARVIVDTY HKTQHKRELP KAP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAC AGATCCAACG CGACATCGCC CAAGCCCTGC AGGTCCAGCC GCCGTTCCAG TCGGAGGCCG ACGTGCAGGC GCAGATCGCC CGGCGCATCG CCTTCATCCA GCAGTGCCTG AAGGATTCCG GGCTGAAGAC CCTGGTGCTG GGGATCAGCG GCGGCGTCGA CTCGCTCACC GCCGGCCTGC TGGCCCAGCG CGCCGTCGAG CAACTGCGCG AGCAGACCGG CGACCAGGCC TACCGCTTCA TCGCCGTGCG CCTGCCCTAC CAGGTGCAGC AGGACGAGGC CGATGCCCAG GCCTCGCTGG CGACCATCCG CGCCGACGAA GAGCAGACCG TCAACATCGG TCCGTCGGTG AAAGCCCTGG CGGAACAGCT GGAAGCCCTG GAAGGACTCG AGCCGGCGAA GAGCGACTTC GTCATCGGCA ATATCAAGGC GCGCATCCGC ATGGTCGCCC AGTACGCCAT CGCCGGCGCC CGCGGCGGCC TGGTGATCGG CACCGACCAT GCTGCCGAGG CGGTCATGGG GTTCTTCACC AAGTTCGGCG ACGGCGCCTG CGACCTGGCT CCGCTCAGCG GCCTGGCCAA GCATCAGGTA CGCGCCCTCG CCCGCGCCCT CGGCGCTCCG GAGAACCTGG TGGAGAAGAT CCCCACCGCC GACCTCGAGG ACCTGCGCCC CGGCCATCCG GACGAGGCTT CCCACGGCGT CACCTATGCC GAGATCGACG CCTTCCTGCA CGGCCAGCCG CTGCGCGAGG AAGCTGCGCG GGTGATCGTC GACACCTACC ACAAGACCCA GCACAAGCGC GAACTGCCGA AGGCGCCCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT
Details for PsaeA.00025.a.B1.PW37555
| PURIFICATION DATe: | 9/9/2014 |
| CONCENTRATION: | 47mg/ml |
| OBSERVED MW: | 30kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ QIQRDIAQAL QVQPPFQSEA DVQAQIARRI AFIQQCLKDS GLKTLVLGIS GGVDSLTAGL LAQRAVEQLR EQTGDQAYRF IAVRLPYQVQ QDEADAQASL ATIRADEEQT VNIGPSVKAL AEQLEALEGL EPAKSDFVIG NIKARIRMVA QYAIAGARGG LVIGTDHAAE AVMGFFTKFG DGACDLAPLS GLAKHQVRAL ARALGAPENL VEKIPTADLE DLRPGHPDEA SHGVTYAEID AFLHGQPLRE EAARVIVDTY HKTQHKRELP KAP
Validated NT Sequence
tactcactta gggcgccttc ggcagttcgc gcttgtgctg ggtcttgtgg taggtgtcga cgatcacccg cgcagcttcc tcgcgcagcg gctggccgtg caggaaggcg tcgatctcgg cataggtgac gccgtgggaa gcctcgtccg gatggccggg gcgcaggtcc tcgaggtcgg cggtggggat cttctccacc aggttctccg gagcgccgag ggcgcgggcg agggcgcgta cctgatgctt ggccaggccg ctgagcggag ccaggtcgca ggcgccgtcg ccgaacttgg tgaagaaccc catgaccgcc tcggcagcat ggtcggtgcc gatcaccagg ccgccgcggg cgccggcgat ggcgtactgg gcgaccatgc ggatgcgcgc cttgatattg ccgatgacga agtcgctctt cgccggctcg agtccttcca gggcttccag ctgttccgcc agggctttca ccgacggacc gatgttgacg gtctgctctt cgtcggcgcg gatggtcgcc agcgaggcct gggcatcggc ctcgtcctgc tgcacctggt agggcaggcg cacggcgatg aagcggtagg cctggtcgcc ggtctgctcg cgcagttgct cgacggcgcg ctgggccagc aggccggcgg tgagcgagtc gacgccgccg ctgatcccca gcaccagggt cttcagcccg gaatccttca ggcactgctg gatgaaggcg atgcgccggg cgatctgcgc ctgcacgtcg gcctccgact ggaacggcgg ctggacctgc agggcttggg cgatgtcgcg ttggatctgt tgcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMQ QIQRDIAQAL QVQPPFQSEA DVQAQIARRI AFIQQCLKDS GLKTLVLGIS GGVDSLTAGL LAQRAVEQLR EQTGDQAYRF IAVRLPYQVQ QDEADAQASL ATIRADEEQT VNIGPSVKAL AEQLEALEGL EPAKSDFVIG NIKARIRMVA QYAIAGARGG LVIGTDHAAE AVMGFFTKFG DGACDLAPLS GLAKHQVRAL ARALGAPENL VEKIPTADLE DLRPGHPDEA SHGVTYAEID AFLHGQPLRE EAARVIVDTY HKTQHKRELP KAP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAC AGATCCAACG CGACATCGCC CAAGCCCTGC AGGTCCAGCC GCCGTTCCAG TCGGAGGCCG ACGTGCAGGC GCAGATCGCC CGGCGCATCG CCTTCATCCA GCAGTGCCTG AAGGATTCCG GGCTGAAGAC CCTGGTGCTG GGGATCAGCG GCGGCGTCGA CTCGCTCACC GCCGGCCTGC TGGCCCAGCG CGCCGTCGAG CAACTGCGCG AGCAGACCGG CGACCAGGCC TACCGCTTCA TCGCCGTGCG CCTGCCCTAC CAGGTGCAGC AGGACGAGGC CGATGCCCAG GCCTCGCTGG CGACCATCCG CGCCGACGAA GAGCAGACCG TCAACATCGG TCCGTCGGTG AAAGCCCTGG CGGAACAGCT GGAAGCCCTG GAAGGACTCG AGCCGGCGAA GAGCGACTTC GTCATCGGCA ATATCAAGGC GCGCATCCGC ATGGTCGCCC AGTACGCCAT CGCCGGCGCC CGCGGCGGCC TGGTGATCGG CACCGACCAT GCTGCCGAGG CGGTCATGGG GTTCTTCACC AAGTTCGGCG ACGGCGCCTG CGACCTGGCT CCGCTCAGCG GCCTGGCCAA GCATCAGGTA CGCGCCCTCG CCCGCGCCCT CGGCGCTCCG GAGAACCTGG TGGAGAAGAT CCCCACCGCC GACCTCGAGG ACCTGCGCCC CGGCCATCCG GACGAGGCTT CCCACGGCGT CACCTATGCC GAGATCGACG CCTTCCTGCA CGGCCAGCCG CTGCGCGAGG AAGCTGCGCG GGTGATCGTC GACACCTACC ACAAGACCCA GCACAAGCGC GAACTGCCGA AGGCGCCCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT