PsaeA.00023.a
Inorganic pyrophosphatase (EC 3.6.1.1) (Pyrophosphate phospho-hydrolase) (PPase)
| CENTER ID: | PsaeA.00023.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.00023.a.B1.GE38188 | full length | 1 | 175 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4XEL
DEPOSITED: 12/24/2014
DETERMINATION: XRay
CLONE: PsaeA.00023.a.B1.GE38188
PROTEIN: PsaeA.00023.a.B1.PS02158
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.4222 |
| PGAT: | pseudomonas:PA4031 |
| RefSeq: | NP_252720.1 |
| UniProt: | Q9HWZ6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSYSKIPAGK DLPNDIYVAI EIPANHAPIK YEIDKDTDCL FVDRFMATPM FYPANYGFIP NTLADDGDPL DVLVVTPYPV APGSVIRARP VGVLHMTDEA GGDAKLIAVP HDKLSVLYKD VKEYTDLPAL LLEQIKHFFE NYKDLEKGKW VKVEGWGNAD AARAEITKAV AAFQK
NT Sequence
atgagctaca gcaagatccc ggccggcaag gacctgccga acgacatcta cgtcgctatc gagattccgg ccaaccatgc gccgatcaag tacgagatcg acaaggacac cgactgcctg ttcgtcgacc gtttcatggc caccccgatg ttctacccgg ccaactacgg cttcatcccg aacactctgg ccgacgacgg cgacccgctg gacgtgctgg tggtgacccc ctatccggtc gctcccggtt cggtgatccg cgcgcgcccg gttggcgtcc tgcacatgac cgacgaggca ggcggcgacg ccaagctgat cgccgtgccg cacgacaagc tgagcgtcct gtacaaggac gtgaaggaat acaccgacct gccggccctg ctgctggaac agatcaagca cttcttcgag aactacaagg atctcgagaa aggcaagtgg gtcaaggtcg aaggctgggg caacgccgac gccgcccgcg ccgagatcac caaggccgtg gccgcgttcc agaagtaa
Details for PsaeA.00023.a.B1.GE38188
| HARVESTED ON: | 6/11/2014 |
| SEQUENCED ON: | 6/12/2014 |
| EXPECTED MW: | 20kDa |
| OBSERVED MW: | 20kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS YSKIPAGKDL PNDIYVAIEI PANHAPIKYE IDKDTDCLFV DRFMATPMFY PANYGFIPNT LADDGDPLDV LVVTPYPVAP GSVIRARPVG VLHMTDEAGG DAKLIAVPHD KLSVLYKDVK EYTDLPALLL EQIKHFFENY KDLEKGKWVK VEGWGNADAA RAEITKAVAA FQK
Validated NT Sequence
tagcagccgg atcctatctt actcacttac ttctggaacg cggccacggc cttggtgatc tcggcgcggg cggcgtcggc gttgccccag ccttcgacct tgacccactt gcctttctcg agatccttgt agttctcgaa gaagtgcttg atctgttcca gcagcagggc cggcaggtcg gtgtattcct tcacgtcctt gtacaggacg ctcagcttgt cgtgcggcac ggcgatcagc ttggcgtcgc cgcctgcctc gtcggtcatg tgcaggacgc caaccgggcg cgcgcggatc accgaaccgg gagcgaccgg atagggggtc accaccagca cgtccagcgg gtcgccgtcg tcggccagag tgttcgggat gaagccgtag ttggccgggt agaacatcgg ggtggccatg aaacggtcga cgaacaggca gtcggtgtcc ttgtcgatct cgtacttgat cggcgcatgg ttggccggaa tctcgatagc gacgtagatg tcgttcggca ggtccttgcc ggccgggatc ttgctgtagc tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMS YSKIPAGKDL PNDIYVAIEI PANHAPIKYE IDKDTDCLFV DRFMATPMFY PANYGFIPNT LADDGDPLDV LVVTPYPVAP GSVIRARPVG VLHMTDEAGG DAKLIAVPHD KLSVLYKDVK EYTDLPALLL EQIKHFFENY KDLEKGKWVK VEGWGNADAA RAEITKAVAA FQK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagct acagcaagat cccggccggc aaggacctgc cgaacgacat ctacgtcgct atcgagattc cggccaacca tgcgccgatc aagtacgaga tcgacaagga caccgactgc ctgttcgtcg accgtttcat ggccaccccg atgttctacc cggccaacta cggcttcatc ccgaacactc tggccgacga cggcgacccg ctggacgtgc tggtggtgac cccctatccg gtcgctcccg gttcggtgat ccgcgcgcgc ccggttggcg tcctgcacat gaccgacgag gcaggcggcg acgccaagct gatcgccgtg ccgcacgaca agctgagcgt cctgtacaag gacgtgaagg aatacaccga cctgccggcc ctgctgctgg aacagatcaa gcacttcttc gagaactaca aggatctcga gaaaggcaag tgggtcaagg tcgaaggctg gggcaacgcc gacgccgccc gcgccgagat caccaaggcc gtggccgcgt tccagaagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat