PlyoA.18549.a

Ferredoxin-fold anticodon binding domain, putative

CENTER ID: PlyoA.18549.a
ORGANISM: Plasmodium yoelii yoelii
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlyoA.18549.a.B1.GE38480 full length 1 488
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PY04422
RefSeq: XP_724716.1
UniProt: Q7RGC5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKLPKIILT NEGKTLYNIL NHPIRTVKSR IEYFFKFENF DNLNNEISVK DNFDHLFVPL KHPVRNFKDT FYLNENYIKN YSYIQKYYYD IYEKLNPFYK YYLANKFLHH DQIKLKRTHM TTHLPELLRT NHKQVIYTGT VYRRDQIDKL HFPIFHQTDG FLIHHDTFDV ESELKLKLEN LISHLFGSKS IQMQWDHQTS FPFTEPSFEL YIKQNLDNNN NNNNNNNNNK NKNNIIVSNP QLVTSQPNGC TTQENHDDSK NWVEVLGCGK IKKEVIAISL YENEINKIIE KEISLFAPNL LKDIFKFSNE KNIDISLPSV LNVSNKLCND DLNKKIEIKI NEFLKTIKYN GWAFGIGLER LAMLLYDIND IRLFWSQDNR FINQFKENEI SKFIPFSNFP SIHKDISFYA NENFDEALFF QICRDIANEN IEQVKKIDHY FNQKTKQTSL CYRIIYRSHF QNLTHKNINE TQNKIIQALT KECFITIR
NT Sequence
atggcaaaat tgcctaaaat aattttaacc aatgaaggta aaaccctata caatatttta aatcatccaa taaggacagt aaaatctcga attgaatatt tttttaagtt tgaaaatttt gataatttga ataatgaaat atcagtaaaa gataattttg atcatctttt tgttccttta aaacatcctg ttcgaaattt taaagataca ttttatttaa atgaaaatta tataaaaaat tattcttata tacaaaaata ttattatgat atatatgaaa aattaaatcc tttttataaa tattatttag ctaataaatt tttacatcat gatcaaatta aattaaaaag aacacacatg actacacatt tacctgaact gttaagaaca aatcataaac aggttatata tacaggtaca gtatatagaa gagatcaaat agataaattg cattttccta tttttcacca aacagatggc tttttaatac atcatgatac ttttgatgta gaatccgaat taaagctaaa attggaaaat ttaatttccc atttgtttgg gtcaaaatct atacaaatgc aatgggacca tcaaacatct tttcctttta cagaaccgtc cttcgaattg tacataaaac aaaatttaga caataataat aataataata ataataataa taataataaa aataaaaata atattattgt ctcaaatcca caactcgtaa catcacaacc taatgggtgc actacacaag aaaatcatga cgactcaaaa aactgggtcg aggttttagg ttgtggaaaa ataaaaaaag aagtaattgc aatttcatta tatgaaaatg aaataaataa aattatagaa aaagaaatct ctttatttgc accaaattta ttgaaagata tttttaaatt ttcaaatgaa aaaaatattg acatttcttt accatctgtt cttaatgtat caaataaatt gtgtaacgat gatcttaata aaaaaatcga aattaaaatt aacgaatttt taaaaacaat caaatataat ggatgggcat ttgggatagg ccttgaaaga ttggctatgt tattatacga tattaatgat atacgattat tttggagcca agataataga tttataaatc agtttaaaga aaatgaaatc tcaaaattta taccattcag caattttcca tcaattcata aagatatatc attttatgca aatgaaaatt ttgatgaagc attatttttt caaatatgtc gagatatagc taatgaaaat attgaacaag ttaaaaaaat tgatcattat tttaatcaaa aaacaaaaca aacaagttta tgctatagaa taatatatag atcacatttt caaaacttaa cacataaaaa tataaatgaa acacaaaata aaattataca agcattaacc aaggaatgtt ttattaccat tagatag
Details for PlyoA.18549.a.B1.GE38480
HARVESTED ON: 10/9/2014
SEQUENCED ON: 11/24/2014
EXPECTED MW: 59kDa
OBSERVED MW: 59kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMA KLPKIILTNE GKTLYNILNH PIRTVKSRIE YFFKFENFDN LNNEISVKDN FDHLFVPLKH PVRNFKDTFY LNENYIKNYS YIQKYYYDIY EKLNPFYKYY LANKFLHHDQ IKLKRTHMTT HLPELLRTNH KQVIYTGTVY RRDQIDKLHF PIFHQTDGFL IHHDTFDVES ELKLKLENLI SHLFGSKSIQ MQWDHQTSFP FTEPSFELYI KQNLDNNNNN NNNNNNNKNK NNIIVSNPQL VTSQPNGCTT QENHDDSKNW VEVLGCGKIK KEVIAISLYE NEINKIIEKE ISLFAPNLLK DIFKFSNEKN IDISLPSVLN VSNKLCNDDL NKKIEIKINE FLKTIKYNGW AFGIGLERLA MLLXXINDIR LFWSQDNRFI NQFKENEISK FIPFSNFPSI HKDISFYANE NFDEALFFQI CRDIANENIE QVKKIDHYFN QKTKQTSLCY RIIYRSHFQN LTHKNXXETQ NKIIQ
Validated NT Sequence
gcttgtataa ttttattttg tgtttcatnn atatttttat gtgttaagtt ttgaaaatgt gatctatata ttattctata gcataaactt gtttgttttg ttttttgatt aaaataatga tcaatttttt taacttgttc aatattttca ttagctatat ctcgacatat ttgaaaaaat aatgcttcat caaaattttc atttgcataa aatgatatat ctttatgaat tgatggaaaa ttgctgaatg gtataaattt tgagatttca ttttctttaa actgatttat aaatctatta tcttggctcc aaaataatcg tatatcatta atannntata ataacatagc caatctttca aggcctatcc caaatgccca tccattatat ttgattgttt ttaaaaattc gttaatttta atttcgattt ttttattaag atcatcgtta cacaatttat ttgatacatt aagaacagat ggtaaagaaa tgtcaatatt tttttcattt gaaaatttaa aaatatcttt caataaattt ggtgcaaata aagagatttc tttttctata attttattta tttcattttc atataatgaa attgcaatta cttctttttt tatttttcca caacctaaaa cctcgaccca gttttttgag tcgtcatgat tttcttgtgt agtgcaccca ttaggttgtg atgttacgag ttgtggattt gagacaataa tattattttt atttttatta ttattattat tattattatt attattatta ttgtctaaat tttgttttat gtacaattcg aaggacggtt ctgtaaaagg aaaagatgtt tgatggtccc attgcatttg tatagatttt gacccaaaca aatgggaaat taaattttcc aattttagct ttaattcgga ttctacatca aaagtatcat gatgtattaa aaagccatct gtttggtgaa aaataggaaa atgcaattta tctatttgat ctcttctata tactgtacct gtatatataa cctgtttatg atttgttctt aacagttcag gtaaatgtgt agtcatgtgt gttcttttta atttaatttg atcatgatgt aaaaatttat tagctaaata atatttataa aaaggattta atttttcata tatatcataa taatattttt gtatataaga ataatttttt atataatttt catttaaata aaatgtatct ttaaaatttc gaacaggatg ttttaaagga acaaaaagat gatcaaaatt atcttttact gatatttcat tattcaaatt atcaaaattt tcaaacttaa aaaaatattc aattcgagat tttactgtcc ttattggatg atttaaaata ttgtataggg ttttaccttc attggttaaa attattttag gcaattttgc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMA KLPKIILTNE GKTLYNILNH PIRTVKSRIE YFFKFENFDN LNNEISVKDN FDHLFVPLKH PVRNFKDTFY LNENYIKNYS YIQKYYYDIY EKLNPFYKYY LANKFLHHDQ IKLKRTHMTT HLPELLRTNH KQVIYTGTVY RRDQIDKLHF PIFHQTDGFL IHHDTFDVES ELKLKLENLI SHLFGSKSIQ MQWDHQTSFP FTEPSFELYI KQNLDNNNNN NNNNNNNKNK NNIIVSNPQL VTSQPNGCTT QENHDDSKNW VEVLGCGKIK KEVIAISLYE NEINKIIEKE ISLFAPNLLK DIFKFSNEKN IDISLPSVLN VSNKLCNDDL NKKIEIKINE FLKTIKYNGW AFGIGLERLA MLLYDINDIR LFWSQDNRFI NQFKENEISK FIPFSNFPSI HKDISFYANE NFDEALFFQI CRDIANENIE QVKKIDHYFN QKTKQTSLCY RIIYRSHFQN LTHKNINETQ NKIIQALTKE CFITIR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa aattgcctaa aataatttta accaatgaag gtaaaaccct atacaatatt ttaaatcatc caataaggac agtaaaatct cgaattgaat atttttttaa gtttgaaaat tttgataatt tgaataatga aatatcagta aaagataatt ttgatcatct ttttgttcct ttaaaacatc ctgttcgaaa ttttaaagat acattttatt taaatgaaaa ttatataaaa aattattctt atatacaaaa atattattat gatatatatg aaaaattaaa tcctttttat aaatattatt tagctaataa atttttacat catgatcaaa ttaaattaaa aagaacacac atgactacac atttacctga actgttaaga acaaatcata aacaggttat atatacaggt acagtatata gaagagatca aatagataaa ttgcattttc ctatttttca ccaaacagat ggctttttaa tacatcatga tacttttgat gtagaatccg aattaaagct aaaattggaa aatttaattt cccatttgtt tgggtcaaaa tctatacaaa tgcaatggga ccatcaaaca tcttttcctt ttacagaacc gtccttcgaa ttgtacataa aacaaaattt agacaataat aataataata ataataataa taataataat aaaaataaaa ataatattat tgtctcaaat ccacaactcg taacatcaca acctaatggg tgcactacac aagaaaatca tgacgactca aaaaactggg tcgaggtttt aggttgtgga aaaataaaaa aagaagtaat tgcaatttca ttatatgaaa atgaaataaa taaaattata gaaaaagaaa tctctttatt tgcaccaaat ttattgaaag atatttttaa attttcaaat gaaaaaaata ttgacatttc tttaccatct gttcttaatg tatcaaataa attgtgtaac gatgatctta ataaaaaaat cgaaattaaa attaacgaat ttttaaaaac aatcaaatat aatggatggg catttgggat aggccttgaa agattggcta tgttattata cgatattaat gatatacgat tattttggag ccaagataat agatttataa atcagtttaa agaaaatgaa atctcaaaat ttataccatt cagcaatttt ccatcaattc ataaagatat atcattttat gcaaatgaaa attttgatga agcattattt tttcaaatat gtcgagatat agctaatgaa aatattgaac aagttaaaaa aattgatcat tattttaatc aaaaaacaaa acaaacaagt ttatgctata gaataatata tagatcacat tttcaaaact taacacataa aaatataaat gaaacacaaa ataaaattat acaagcatta accaaggaat gttttattac cattagatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at