SSGCID: Target Page

PlyoA.18543.a

Putative uncharacterized protein PY06925

CENTER ID:	PlyoA.18543.a
ORGANISM:	Plasmodium yoelii yoelii
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlyoA.18543.a.B1.GE38319	full length		1	283

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PY06925
RefSeq:	XP_727684.1
UniProt:	Q7R9D7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLDLEKNAKK KIDLTKLPEH FQDLGIDYLT TIRPAFFHKY INDGNENPMN EGLEISYDKN ELDSINENNE NSDLSKSNNN NDNNNDNNDN DESTDDKQKR NIVISSQQIV MHNRNTNDEV DELQNEEYYN NMNKEKALDI LMKNTSLHME GICITLKNNE NKYVGQCKAK MNKHFSSIAC ESYENSYLRR NHQKKELSSG MMNEELNNRM SEMMKFFENP EEAAGMLLSA LSKPGFMESI NALDIASHFN FDQPHEDMNP EDLFNLDNNP SDDTDKDQTN LDN

NT Sequence

atgctagatt tagaaaaaaa tgctaaaaaa aaaatcgatt taacaaaatt accagaacat tttcaagatt taggaataga ctatttgact acaataaggc ctgcattttt ccataaatat attaatgatg gaaatgaaaa tccaatgaat gaaggattag aaataagtta tgataaaaat gaacttgatt caataaatga aaataatgaa aattcagatt tatcaaaatc gaataataat aatgataata ataatgataa taatgataat gatgaatcta ctgatgataa acaaaaaaga aacattgtta tatcatcaca acaaattgta atgcataata gaaatacaaa tgatgaagtt gatgaattac aaaatgaaga atattataat aatatgaata aagaaaaagc tttagatata ttaatgaaaa atacaagttt acacatggaa ggaatatgta ttacattaaa aaataatgaa aataaatatg ttggtcaatg taaagctaaa atgaataaac attttagttc aattgcttgt gaatcttatg aaaatagtta tttaagaaga aatcaccaaa aaaaagaatt atcttcaggc atgatgaatg aagaattaaa taatagaatg tcagaaatga tgaaattttt tgaaaaccca gaggaagctg ctggtatgtt attatctgct ttaagtaaac ccggatttat ggaatcaata aatgctttag atatagcatc tcattttaat tttgaccaac cacatgaaga tatgaatcca gaagatttat tcaatttaga taataaccca agtgatgaca ctgataaaga tcaaacaaat ttagataatt aa

Details for PlyoA.18543.a.B1.GE38319

HARVESTED ON:	7/21/2014
SEQUENCED ON:	7/26/2014
EXPECTED MW:	34kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML DLEKNAKKKI DLTKLPEHFQ DLGIDYLTTI RPAFFHKYIN DGNENPMNEG LEISYDKNEL DSINENNENS DLSKSNNNND NNNDNNDNDE STDDKQKRNI VISSQQIVMH NRNTNDEVDE LQNEEYYNNM NKEKALDILM KNTSLHMEGI CITLKNNENK YVGQCKAKMN KHFSSIACES YENSYLRRNH QKKELSSGMM NEELNNRMSE MMKFFENPEE AAGMLLSALS KPGFMESINA LDIASHFNFD QPHEDMNPED LFNLDNNPSD DTDKDQTNLD N

Validated NT Sequence

cnctananat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctag atttagaaaa aaatgctaaa aaaaaaatcg atttaacaaa attaccagaa cattttcaag atttaggaat agactatttg actacaataa ggcctgcatt tttccataaa tatattaatg atggaaatga aaatccaatg aatgaaggat tagaaataag ttatgataaa aatgaacttg attcaataaa tgaaaataat gaaaattcag atttatcaaa atcgaataat aataatgata ataataatga taataatgat aatgatgaat ctactgatga taaacaaaaa agaaacattg ttatatcatc acaacaaatt gtaatgcata atagaaatac aaatgatgaa gttgatgaat tacaaaatga agaatattat aataatatga ataaagaaaa agctttagat atattaatga aaaatacaag tttacacatg gaaggaatat gtattacatt aaaaaataat gaaaataaat atgttggtca atgtaaagct aaaatgaata aacattttag ttcaattgct tgtgaatctt atgaaaatag ttatttaaga agaaatcacc aaaaaaaaga attatcttca ggcatgatga atgaagaatt aaataataga atgtcagaaa tgatgaaatt ttttgaaaac ccagaggaag ctgctggtat gttattatct gctttaagta aacccggatt tatggaatca ataaatgctt tagatatagc atctcatttt aattttgacc aaccacatga agatatgaat ccagaagatt tattcaattt agataataac ccaagtgatg acactgataa agatcaaaca aatttagata attaagtgag taagatagga tccnntnccc ccccnccnna nannnagnnn ngnngnnnna aaanntnnnn cnannaaaaa nnnttcctan aggcnggann cnnggtcnna cgtcagancc actnccgcnt cgagnnanca tccntnnnna ncgggcccag tanant

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctag atttagaaaa aaatgctaaa aaaaaaatcg atttaacaaa attaccagaa cattttcaag atttaggaat agactatttg actacaataa ggcctgcatt tttccataaa tatattaatg atggaaatga aaatccaatg aatgaaggat tagaaataag ttatgataaa aatgaacttg attcaataaa tgaaaataat gaaaattcag atttatcaaa atcgaataat aataatgata ataataatga taataatgat aatgatgaat ctactgatga taaacaaaaa agaaacattg ttatatcatc acaacaaatt gtaatgcata atagaaatac aaatgatgaa gttgatgaat tacaaaatga agaatattat aataatatga ataaagaaaa agctttagat atattaatga aaaatacaag tttacacatg gaaggaatat gtattacatt aaaaaataat gaaaataaat atgttggtca atgtaaagct aaaatgaata aacattttag ttcaattgct tgtgaatctt atgaaaatag ttatttaaga agaaatcacc aaaaaaaaga attatcttca ggcatgatga atgaagaatt aaataataga atgtcagaaa tgatgaaatt ttttgaaaac ccagaggaag ctgctggtat gttattatct gctttaagta aacccggatt tatggaatca ataaatgctt tagatatagc atctcatttt aattttgacc aaccacatga agatatgaat ccagaagatt tattcaattt agataataac ccaagtgatg acactgataa agatcaaaca aatttagata attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat