PlyoA.18499.a

Putative uncharacterized protein PY00704

CENTER ID: PlyoA.18499.a
ORGANISM: Plasmodium yoelii yoelii
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlyoA.18499.a.B1.GE38318 full length 1 337
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PY00704
RefSeq: XP_726399.1
UniProt: Q7RRL5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSVSTLPYI GSKISLISNS EIRYEGILYT INTHESTVAL QNVRSYGTEG RRQPDIPASN EVYDFIIFRG KDIKDVTVSE AAKTIPDDPA IVSMNIAPST KNNIGDNLNY NNNVNNINKP LNVQNNMIPQ NERNMNLNNR RYYNRHNYNY YYNGNIGNGN MNGNPNYNNN HNNNRHYNNF KYKNYRNYDR QPYVIGELES QPNPVLKSKF SPDFDFSSNN LKFDKTNIID EKSKDVLTAN SNIQVGGYDK KSSFFDNISC ETLDKQQGKD EKVDREKLRM LDVDTFGIAA AHYRTNMHNR NNNRNKGRNA KNNKMMGNFN YNYYNRNQNP FNRYPAY
NT Sequence
atgtcttctg tatcaacatt accatatata ggaagtaaaa tttctttaat ttcaaactca gaaattagat atgaaggaat tttgtatacc ataaacaccc atgaatctac tgttgcattg caaaatgtta gatcatatgg cacagaaggt agaagacagc ctgatattcc tgcatcaaat gaagtatatg attttataat atttcgaggg aaagatataa aagatgttac agtaagtgaa gccgctaaaa cgatacctga tgatccagca atagtatcaa tgaatattgc gccatcaacg aaaaataata taggggataa tttaaattat aacaataatg taaacaatat taataaacca ctaaatgttc aaaataatat gatcccacaa aatgaaagaa atatgaattt aaataataga agatattata ataggcataa ttataattat tattataatg gtaatattgg aaatggtaat atgaatggca atccaaacta caataataat cataataaca ataggcatta taataatttt aaatataaaa attatcgaaa ttatgataga caaccatatg ttattggaga attggaatca cagccaaatc cagtattaaa aagtaaattt agtccagatt ttgattttag ctcaaataat ttaaaatttg acaaaacgaa tataatagat gaaaaaagca aagatgtatt aactgcaaat agtaacatac aggtaggagg atatgataaa aagtctagtt tttttgataa tattagttgt gaaacattag ataaacaaca aggaaaagat gaaaaggtgg acagagaaaa attaaggatg ctagatgttg atacttttgg tatagcggct gcacattata ggactaatat gcataataga aataataaca gaaataaagg aagaaatgct aagaataata aaatgatggg aaactttaat tataattatt ataacagaaa ccaaaatcca tttaacagat atccagccta ttaa
Details for PlyoA.18499.a.B1.GE38318
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 40kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 84
Validated AA Sequence
MAHHHHHHMS SVSTLPYIGS KISLISNSEI RYEGILYTIN THESTVALQN VRSYGTEGRR QPDIPASNEV YDFIIFRGKD IKDVTVSEAA KTIPDDPAIV SMNIAPSTKN NIGDNLNYNN NVNNINKPLN VQNNMIPQNE RNMNLNNRRY YNRHNYNYYY NGNIGNGNMN GNPNYNNNHN NNRHYNNFKY KNYRNYDRQP YVIGELESQP NPVLKSKFSP DFDFSSNNLK FDKTNIIDEK SKDVLTANSN IQVGGYDKKS SFFDNISCET LDKQQGKDEK VDREKLKDAR C
Validated NT Sequence
cnctanannt tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt ctgtatcaac attaccatat ataggaagta aaatttcttt aatttcaaac tcagaaatta gatatgaagg aattttgtat accataaaca cccatgaatc tactgttgca ttgcaaaatg ttagatcata tggcacagaa ggtagaagac agcctgatat tcctgcatca aatgaagtat atgattttat aatatttcga gggaaagata taaaagatgt tacagtaagt gaagccgcta aaacgatacc tgatgatcca gcaatagtat caatgaatat tgcgccatca acgaaaaata atatagggga taatttaaat tataacaata atgtaaacaa tattaataaa ccactaaatg ttcaaaataa tatgatccca caaaatgaaa gaaatatgaa tttaaataat agaagatatt ataataggca taattataat tattattata atggtaatat tggaaatggt aatatgaatg gcaatccaaa ctacaataat aatcataata acaataggca ttataataat tttaaatata aaaattatcg aaattatgat agacaaccat atgttattgg agaattggaa tcacagccaa atccagtatt aaaaagtaaa tttagtccag attttgattt tagctcaaat aatttaaaat ttgacaaaac gaatataata gatgaaaaaa gcaaagatgt attaactgca aatagtaaca tacaggtagg aggatatgat aaaaagtcta gtttttttga taatattagt tgtgaaacat tagataaaca acaaggaaaa gatgaaaagg tggacagaga aaaattaaag gatgctagat gttgatactt ttggtatagc ggctgcacat tataggacta atatgcataa tagaaataat aacngaaata aaggaagaaa tgctaagaat aataaaatga tgggaaactt taattataat tattatnacn gaaaccaaaa tccatttaac nganntcann nattngtgag taagnnnnnc nnnnnncncc ccnnnnnnnn nnnnnnnnga nnngnnnnnn nnnnngaann aanaananat cnnnnnnnnn nnnnnnnnnn na
Expected Protein Sequence
MAHHHHHHMS SVSTLPYIGS KISLISNSEI RYEGILYTIN THESTVALQN VRSYGTEGRR QPDIPASNEV YDFIIFRGKD IKDVTVSEAA KTIPDDPAIV SMNIAPSTKN NIGDNLNYNN NVNNINKPLN VQNNMIPQNE RNMNLNNRRY YNRHNYNYYY NGNIGNGNMN GNPNYNNNHN NNRHYNNFKY KNYRNYDRQP YVIGELESQP NPVLKSKFSP DFDFSSNNLK FDKTNIIDEK SKDVLTANSN IQVGGYDKKS SFFDNISCET LDKQQGKDEK VDREKLRMLD VDTFGIAAAH YRTNMHNRNN NRNKGRNAKN NKMMGNFNYN YYNRNQNPFN RYPAY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt ctgtatcaac attaccatat ataggaagta aaatttcttt aatttcaaac tcagaaatta gatatgaagg aattttgtat accataaaca cccatgaatc tactgttgca ttgcaaaatg ttagatcata tggcacagaa ggtagaagac agcctgatat tcctgcatca aatgaagtat atgattttat aatatttcga gggaaagata taaaagatgt tacagtaagt gaagccgcta aaacgatacc tgatgatcca gcaatagtat caatgaatat tgcgccatca acgaaaaata atatagggga taatttaaat tataacaata atgtaaacaa tattaataaa ccactaaatg ttcaaaataa tatgatccca caaaatgaaa gaaatatgaa tttaaataat agaagatatt ataataggca taattataat tattattata atggtaatat tggaaatggt aatatgaatg gcaatccaaa ctacaataat aatcataata acaataggca ttataataat tttaaatata aaaattatcg aaattatgat agacaaccat atgttattgg agaattggaa tcacagccaa atccagtatt aaaaagtaaa tttagtccag attttgattt tagctcaaat aatttaaaat ttgacaaaac gaatataata gatgaaaaaa gcaaagatgt attaactgca aatagtaaca tacaggtagg aggatatgat aaaaagtcta gtttttttga taatattagt tgtgaaacat tagataaaca acaaggaaaa gatgaaaagg tggacagaga aaaattaagg atgctagatg ttgatacttt tggtatagcg gctgcacatt ataggactaa tatgcataat agaaataata acagaaataa aggaagaaat gctaagaata ataaaatgat gggaaacttt aattataatt attataacag aaaccaaaat ccatttaaca gatatccagc ctattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat