SSGCID: Target Page

PlyoA.18471.a

Putative uncharacterized protein PY03661

CENTER ID:	PlyoA.18471.a
ORGANISM:	Plasmodium yoelii yoelii
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlyoA.18471.a.B1.GE38317	full length		1	225

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PY03661
RefSeq:	XP_723848.1
UniProt:	Q7RIG2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFRSDSHFLM VTNLRFLELQ KMLLNRFQIF KNEEIRILKK DNMKRILYEW AKFLTKENIK TNITNIPEKV LENKIVTKII NDDIDCKWLV NKINVKNQNE NNIIKNQELK KIDNLHFDDY PNLFYYEYPQ FPYNYYDKKK MTNFYSLIKF PYNDIINISR KIKTDEETEQ NGQEREKNGQ ETEQHEITDM LHPDKDILNC DNYSCDNYIY APSEMETSKE NYHQT

NT Sequence

atgtttcgat ctgattccca tttcctaatg gtaacaaact tgcggttttt agaactgcaa aaaatgttac taaatagatt ccaaattttt aaaaatgaag aaataagaat attaaaaaaa gataatatga aaagaatttt atatgaatgg gcgaaatttt taacaaaaga aaatataaaa acaaatataa caaatattcc tgaaaaagta ttagaaaata aaatagtgac taaaataata aatgatgata ttgattgtaa atggctagtt aataaaatca atgttaaaaa tcaaaatgaa aataatatca taaaaaatca agaattaaaa aaaattgata atcttcattt tgatgactac cccaatttat tttattacga atacccacaa ttcccatata attattatga caaaaaaaaa atgaccaatt tttattcact tataaaattt ccatataacg atataattaa catttcgaga aaaatcaaaa ctgacgagga gacagaacag aatggtcagg agagagaaaa gaatggtcag gaaaccgaac aacatgaaat cacagacatg ttacatcccg acaaagatat tttaaattgt gataactact cttgtgacaa ttatatttat gctccatcag aaatggaaac atcgaaagaa aattatcatc aaacataa

Details for PlyoA.18471.a.B1.GE38317

HARVESTED ON:	7/21/2014
SEQUENCED ON:	7/26/2014
EXPECTED MW:	28kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

Validated NT Sequence

cnnnnnnttc ntctannntn ttttgtttaa ctttaagaag gagatatacc atgggctcac caccaccacc accatatgtt tcgatctgat tcccatttcc taatggtaac aaacttgcgg tttttagaac tgcaaaaaat gttactaaat agattccaaa tttttaaaaa tgaagaaata agaatattaa aaaaagataa tatgaaaaga attttatatg aatgggcgaa atttttaaca aaagaaaata taaaaacaaa tataacaaat attcctgaaa aagtattaga aaataaaata gtgactaaaa taataaatga tgatattgat tgtaaatggc tagttaataa aatcaatgtt aaaaatcaaa atgaaaataa tatcataaaa aatcaagaat taaaaaaaat tgataatctt cattttgatg actaccccaa tttattttat tacgaatacc cacaattccc atataattat tatgacaaaa aaaaaatgac caatttttat tcacttataa aatttccata taacgatata attaacattt cgagaaaaat caaaactgac gaggagacag aacagaatgg tcaggagaga gaaaagaatg gtcaggaaac cgaacaacat gaaatcacag acatgttaca tcccgacaaa gatattttaa attgtgataa ctactcttgt gacaattata tttatgctcc atcagaaatg gaaacatcga aagaaaatta tcatcaaaca taagtgagta agataggatc cggtcagcnc ccnnnnnnnn nnnngaanaa nnncnnaaaa aaaaatnnnn nnnaaaaaaa aaancngaac gacctgtatc tcnggtgatc aaccncacaa ccgtctgaag cgcctgctca acctggctgc tccagacatc atnatgctcn acgaaagcnn ntgctgcaag angctgtcna cgacctgctc gacacgatca gncgcggtcg cgcnttcacn agcnnggaca gncttcnnct gaaatcccgt ggtgcanatt atcangtgct agccatgncn ntaccntnaa nacactngta ccnnntncng cctgatanan ntcnngnatc nnnnnnnncc cnttgnnccc naccctnnng natgacnnan nnnnggnttn nnnnnaaaan nngcccgcan angcntnnnt tnnntnctgn ntacttnttc tnnnnnnnnn ntaangnnnt cgnnnnnnnn nnnnnnnnnc nancngnnnn nntnnnnnnc ngagnnnncc ncanngntnn ntgnnnna

Expected Protein Sequence

MAHHHHHHMF RSDSHFLMVT NLRFLELQKM LLNRFQIFKN EEIRILKKDN MKRILYEWAK FLTKENIKTN ITNIPEKVLE NKIVTKIIND DIDCKWLVNK INVKNQNENN IIKNQELKKI DNLHFDDYPN LFYYEYPQFP YNYYDKKKMT NFYSLIKFPY NDIINISRKI KTDEETEQNG QEREKNGQET EQHEITDMLH PDKDILNCDN YSCDNYIYAP SEMETSKENY HQT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttc gatctgattc ccatttccta atggtaacaa acttgcggtt tttagaactg caaaaaatgt tactaaatag attccaaatt tttaaaaatg aagaaataag aatattaaaa aaagataata tgaaaagaat tttatatgaa tgggcgaaat ttttaacaaa agaaaatata aaaacaaata taacaaatat tcctgaaaaa gtattagaaa ataaaatagt gactaaaata ataaatgatg atattgattg taaatggcta gttaataaaa tcaatgttaa aaatcaaaat gaaaataata tcataaaaaa tcaagaatta aaaaaaattg ataatcttca ttttgatgac taccccaatt tattttatta cgaataccca caattcccat ataattatta tgacaaaaaa aaaatgacca atttttattc acttataaaa tttccatata acgatataat taacatttcg agaaaaatca aaactgacga ggagacagaa cagaatggtc aggagagaga aaagaatggt caggaaaccg aacaacatga aatcacagac atgttacatc ccgacaaaga tattttaaat tgtgataact actcttgtga caattatatt tatgctccat cagaaatgga aacatcgaaa gaaaattatc atcaaacatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat