PlyoA.18308.a

Aspartyl protease-like

CENTER ID: PlyoA.18308.a
ORGANISM: Plasmodium yoelii yoelii
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlyoA.18308.a.B2.GE35073 aligned to Rhizopus 71 499
PlyoA.18308.a.B3.GE35074 Rhizopus aligned with longer C-term 71 526
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PY02085
RefSeq: XP_729942.1
UniProt: Q7RMU3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGFSKLYNFI IYFIIINILV QANSEDENVL NKNNEKNVLS KNNEQNVLNK NNGQNVLSKN NEQNVLSKNN EKNEEIYKYK LYGDIDEYAY YFMDIEIGTP GQKLSLIVDT GSSSLSFPCS ECKDCGIHME NPFNLNNSST SSVLYCNDNT CPYNLKCVKG RCEYLQSYCE GSRINGFYFS DVVKLESTNN TKSGNITFKK HMGCHMHEEG LFLYQHATGV LGLSLTKPKG VPTFIDLLFK NSPKLNKIFS LCISEYGGEL ILGGYSKDYI VKEVSIDEKK ENIEDNKNEN IDSIDKSVEI NKNKSSVDDI LWEAITRKYY YYIRVEGFQL FGTTFSHNNK SMEMLVDSGS TFTHLPDDLY NNLNFFFDIL CIHNMNNPID IEKRLKITNE TLSKHLLYFD DFKSTLKNII STENVCVKIA DNVQCWRYLK HLPNIYIKLS NNTKLLWQPS SYLYKKESFW CKGLEKQVNN KPILGLSFFK NKQIIFDLKN NKIGFIESNC PSNPINTRPR TFNEYNIKEN HLFKQSYFSL YTFSIILALT FILYIILYIK KFISIYYNPL QGENTSPD
NT Sequence
atgggttttt ctaaattata taattttatt atttatttta taataataaa tattttagtt caagcaaata gtgaagatga aaatgtatta aataaaaata acgaaaaaaa tgtattaagt aaaaataacg agcaaaatgt attaaataaa aataacgggc aaaatgtatt aagtaaaaat aacgagcaaa atgtattaag taaaaataac gaaaaaaatg aggaaatcta taaatataaa ttatatggag atattgacga atatgcatat tattttatgg atatagaaat aggaacacct ggacaaaaac tttcactaat agtagacaca ggatcttctt cattaagttt cccatgttca gaatgtaaag attgtgggat acatatggaa aaccctttta atttaaataa ttcatcaaca tcatcggttt tatattgtaa tgataataca tgtccatata atttaaaatg tgtcaaagga aggtgtgaat atttacaatc ttattgtgag ggatctcgaa ttaacggttt ttatttttcg gatgttgtaa agctcgaatc gaccaataac actaaaagtg gaaacataac ttttaagaaa catatgggat gtcatatgca tgaagaaggt ttatttttat atcaacatgc aacgggggta ttgggattaa gtttaacaaa accaaaaggt gtaccaactt ttattgattt attatttaaa aattccccta aactaaataa aatattttca ttgtgtattt ctgaatatgg aggggaacta attttagggg gatatagtaa agactatatt gtcaaagaag tttcaattga tgaaaagaaa gaaaatatcg aagataataa aaatgaaaat attgattcta ttgataaatc tgtagaaata aataaaaata aatcgagtgt agatgacata ctttgggaag ctataactag aaaatattat tactatataa gagttgaagg gtttcaatta tttggtacaa ctttttctca taataacaaa agtatggaaa tgttagtaga ttctggtagt acctttacac atctacctga cgatttatat aataatttga attttttttt tgatatatta tgtatacaca acatgaacaa tcctattgat atagagaaaa gattaaaaat aacaaatgaa acattaagta agcatctttt atattttgat gattttaaat ctacgctaaa aaatataata agtactgaaa atgtgtgtgt aaaaatagca gataatgtac aatgctggag atatttaaaa catttaccaa atatatatat aaaactgtct aataatacta aactattatg gcaacctagt tcttatttat ataaaaaaga atcattttgg tgtaaaggac tagaaaaaca agtaaataat aaacctattc ttggattaag tttttttaaa aataaacaaa ttatatttga tcttaaaaat aataaaattg ggttcattga atcaaattgc ccatctaatc cgattaatac aagaccgaga acttttaatg aatataatat aaaagaaaat catttattta aacaatcata ttttagctta tatacctttt caataattct tgccttaaca tttatactat acattatttt atacattaaa aaatttatat ctatctatta caacccgttg caaggcgaga acacctcacc cgactaa
Details for PlyoA.18308.a.B2.GE35073
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/11/2012
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHME KNEEIYKYKL YGDIDEYAYY FMDIEIGTPG QKLSLIVDTG SSSLSFPCSE CKDCGIHMEN PFNLNNSSTS SVLYCNDNTC PYNLKCVKGR CEYLQSYCEG SRINGFYFSD VVKLESTNNT KSGNITFKKH MGCHMHEEGL FLYQHATGVL GLSLTKPKGV PTFIDLLFKN SPKLNKIFSL CISEYGGELI LGGYSKDYIV KEVSIDEKKE NIEDNKNENI DSIDKSVEIN KNKSSVDDIL WEAITRKYYY YIRVEGFQLF GTTFSHNNKS MEMLVDSGST FTHLPDDLYN NLNFFFDILC IHNMNNPIDI EKRLKITNET LSKHLLYFDD FKSTLKNIIS TENVCVKIAD NVQCWRYLKH LPNIYIKLSN NTKLLWQPSS YLYKKESFWC KGLEKQVNNK PILGLSFFKN KQIIFDLKNN KIGFIESN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa aaaatgagga aatctataaa tataaattat atggagatat tgacgaatat gcatattatt ttatggatat agaaatagga acacctggac aaaaactttc actaatagta gacacaggat cttcttcatt aagtttccca tgttcagaat gtaaagattg tgggatacat atggaaaacc cttttaattt aaataattca tcaacatcat cggttttata ttgtaatgat aatacatgtc catataattt aaaatgtgtc aaaggaaggt gtgaatattt acaatcttat tgtgagggat ctcgaattaa cggtttttat ttttcggatg ttgtaaagct cgaatcgacc aataacacta aaagtggaaa cataactttt aagaaacata tgggatgtca tatgcatgaa gaaggtttat ttttatatca acatgcaacg ggggtattgg gattaagttt aacaaaacca aaaggtgtac caacttttat tgatttatta tttaaaaatt cccctaaact aaataaaata ttttcattgt gtatttctga atatggaggg gaactaattt tagggggata tagtaaagac tatattgtca aagaagtttc aattgatgaa aagaaagaaa atatcgaaga taataaaaat gaaaatattg attctattga taaatctgta gaaataaata aaaataaatc gagtgtagat gacatacttt gggaagctat aactagaaaa tattattact atataagagt tgaagggttt caattatttg gtacaacttt ttctcataat aacaaaagta tggaaatgtt agtagattct ggtagtacct ttacacatct acctgacgat ttatataata atttgaattt tttttttgat atattatgta tacacaacat gaacaatcct attgatatag agaaaagatt aaaaataaca aatgaaacat taagtaagca tcttttatat tttgatgatt ttaaatctac gctaaaaaat ataataagta ctgaaaatgt gtgtgtaaaa atagcagata atgtacaatg ctggagatat ttaaaacatt taccaaatat atatataaaa ctgtctaata atactaaact attatggcaa cctagttctt atttatataa aaaagaatca ttttggtgta aaggactaga aaaacaagta aataataaac ctattcttgg attaagtttt tttaaaaata aacaaattat atttgatctt aaaaataata aaattgggtt cattgaatca aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PlyoA.18308.a.B3.GE35074
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/11/2012
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 97
PERCENT COVERAGE: 68
Validated AA Sequence
MAHHHHHHME KNEEIYKYKL YGDIDEYAYY FMDIEIGTPG QKLSLIVDTG SSSLSFPCSE CKDCGIHMEN PFNLNNSSTS SVLYCNDNTC PYNLKCVKGR CEYLQSYCEG SRINGFYFSD VVKLESTNNT KSGNITFKKH MGCHMHEEGL FLYQHATGVL GLSLTKPKGV PTFIDLLFKN SPKLNKIFSL CISEYGGELI LGGYSKDYIV KEVSIDEKKE NIEDNKNENI DSIDKSVEIN KNKSSVDDIL WEAITRKYYY YIRVEGFQLF GTTFSHNNXS MEMLVXSGST FTHLPDXLYX NLNFFFXILC IXNMXXPI
Validated NT Sequence
atggctcacc accaccacca ccatatggaa aaaaatgagg aaatctataa atataaatta tatggagata ttgacgaata tgcatattat tttatggata tagaaatagg aacacctgga caaaaacttt cactaatagt agacacagga tcttcttcat taagtttccc atgttcagaa tgtaaagatt gtgggataca tatggaaaac ccttttaatt taaataattc atcaacatca tcggttttat attgtaatga taatacatgt ccatataatt taaaatgtgt caaaggaagg tgtgaatatt tacaatctta ttgtgaggga tctcgaatta acggttttta tttttcggat gttgtaaagc tcgaatcgac caataacact aaaagtggaa acataacttt taagaaacat atgggatgtc atatgcatga agaaggttta tttttatatc aacatgcaac gggggtattg ggattaagtt taacaaaacc aaaaggtgta ccaactttta ttgatttatt atttaaaaat tcccctaaac taaataaaat attttcattg tgtatttctg aatatggagg ggaactaatt ttagggggat atagtaaaga ctatattgtc aaagaagttt caattgatga aaagaaagaa aatatcgaag ataataaaaa tgaaaatatt gattctattg ataaatctgt agaaataaat aaaaataaat cgagtgtaga tgacatactt tgggaagcta taactagaaa atattattac tatataagag ttgaagggtt tcaattattt ggtacaactt tttctcataa taacnaaagt atggaaatgt tagtagantc tggtagtacc tttacacatc tacctgacna tttatatnat aatttgaatt ttttttttna natattatgt atacncaaca tgancnatcc tattg
Expected Protein Sequence
MAHHHHHHME KNEEIYKYKL YGDIDEYAYY FMDIEIGTPG QKLSLIVDTG SSSLSFPCSE CKDCGIHMEN PFNLNNSSTS SVLYCNDNTC PYNLKCVKGR CEYLQSYCEG SRINGFYFSD VVKLESTNNT KSGNITFKKH MGCHMHEEGL FLYQHATGVL GLSLTKPKGV PTFIDLLFKN SPKLNKIFSL CISEYGGELI LGGYSKDYIV KEVSIDEKKE NIEDNKNENI DSIDKSVEIN KNKSSVDDIL WEAITRKYYY YIRVEGFQLF GTTFSHNNKS MEMLVDSGST FTHLPDDLYN NLNFFFDILC IHNMNNPIDI EKRLKITNET LSKHLLYFDD FKSTLKNIIS TENVCVKIAD NVQCWRYLKH LPNIYIKLSN NTKLLWQPSS YLYKKESFWC KGLEKQVNNK PILGLSFFKN KQIIFDLKNN KIGFIESNCP SNPINTRPRT FNEYNIKENH LFKQS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa aaaatgagga aatctataaa tataaattat atggagatat tgacgaatat gcatattatt ttatggatat agaaatagga acacctggac aaaaactttc actaatagta gacacaggat cttcttcatt aagtttccca tgttcagaat gtaaagattg tgggatacat atggaaaacc cttttaattt aaataattca tcaacatcat cggttttata ttgtaatgat aatacatgtc catataattt aaaatgtgtc aaaggaaggt gtgaatattt acaatcttat tgtgagggat ctcgaattaa cggtttttat ttttcggatg ttgtaaagct cgaatcgacc aataacacta aaagtggaaa cataactttt aagaaacata tgggatgtca tatgcatgaa gaaggtttat ttttatatca acatgcaacg ggggtattgg gattaagttt aacaaaacca aaaggtgtac caacttttat tgatttatta tttaaaaatt cccctaaact aaataaaata ttttcattgt gtatttctga atatggaggg gaactaattt tagggggata tagtaaagac tatattgtca aagaagtttc aattgatgaa aagaaagaaa atatcgaaga taataaaaat gaaaatattg attctattga taaatctgta gaaataaata aaaataaatc gagtgtagat gacatacttt gggaagctat aactagaaaa tattattact atataagagt tgaagggttt caattatttg gtacaacttt ttctcataat aacaaaagta tggaaatgtt agtagattct ggtagtacct ttacacatct acctgacgat ttatataata atttgaattt tttttttgat atattatgta tacacaacat gaacaatcct attgatatag agaaaagatt aaaaataaca aatgaaacat taagtaagca tcttttatat tttgatgatt ttaaatctac gctaaaaaat ataataagta ctgaaaatgt gtgtgtaaaa atagcagata atgtacaatg ctggagatat ttaaaacatt taccaaatat atatataaaa ctgtctaata atactaaact attatggcaa cctagttctt atttatataa aaaagaatca ttttggtgta aaggactaga aaaacaagta aataataaac ctattcttgg attaagtttt tttaaaaata aacaaattat atttgatctt aaaaataata aaattgggtt cattgaatca aattgcccat ctaatccgat taatacaaga ccgagaactt ttaatgaata taatataaaa gaaaatcatt tatttaaaca atcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat