PlyoA.01238.a

Seryl-tRNA synthetase

CENTER ID: PlyoA.01238.a
ORGANISM: Plasmodium yoelii yoelii
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlyoA.01238.a.B1.GE37779 full length 1 534
PlyoA.01238.a.B11.GE42704 full length 1 534
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlyoA.01238.a.B1.PW37633 full length 1 534

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PY03295
RefSeq: XP_731290.1
UniProt: Q7RJG5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVLDINLFRT EKGGNPEKIK ESEKNRFNDV SNVDKVIEYD EKWRKSIFKL EELKKNINLI NKTIGNKKKE DKNADVEDLK QKSLSMKEEI PLIQNEEKHL LKQRNMYLAK VGNILSSKVV INNDEDHNKI VRTWGECKNY EATDLEKSDI NKEDTPNTKR EVSNNNDGQN KVSIISEIKK KYYYHYDLLR KIGGVNFKKG VLVAGHRGYY LTGAGFLLNN AIMQYAINFL VNKKYTPVYP PFFMKKTIME ECAELNDFEE TLYKISSSSV ASAEDKTIAT NSNNVSNEKS NRDDLFLIAT SEQPLCALHR DETLESRYLP LKYVGISSCF RKEAGAHGKD IRGILRVHQF DKVEQFCISL PQTSYKLHEE MIKTCEEFYQ SLNIPYKIVN IVSGALNNAA ALKYDIEGYF PTSGQYRELV SCSNCTDYQS INLNIRYTDS NIKISGITKS SKDKNEENTK DETKDEYVDS EYEKFNHDFP IENKNYVHLL NGTMVAGQRF LCCLLENYQN GEGIIVPEKL RPYMNNTEFI PFIE
NT Sequence
atggtcttgg atatcaattt atttagaact gaaaaaggag gtaacccgga aaagatcaaa gagtcagaaa aaaacagatt taatgatgta agtaatgtag ataaagttat agaatatgat gagaaatgga gaaagagtat atttaaatta gaagagttaa aaaaaaacat taacctaata aataaaacaa taggaaataa aaaaaaagaa gataaaaatg cagatgttga ggatttgaaa caaaaaagtt taagcatgaa agaagaaatt ccattgatcc aaaacgaaga aaaacattta ttaaaacaaa gaaatatgta tttagcaaaa gtaggaaata tattaagtag taaagttgta attaataatg atgaagacca taacaaaatt gtacgaacat ggggagaatg caaaaattat gaggcaaccg atttagaaaa aagtgacata aacaaagaag atactcctaa tacaaaaaga gaagtgagca ataataatga tggacaaaat aaagtcagta taataagtga aataaaaaag aaatattatt atcattatga tttgctaaga aaaatagggg gagtgaattt taagaaaggg gttttagtag ctggacatag aggttattat cttactggtg caggcttttt attaaataat gctattatgc aatatgctat aaattttctt gtaaataaaa aatatacacc cgtataccct ccgtttttta tgaaaaaaac cataatggag gaatgtgcag agttaaacga ttttgaagaa acattatata aaataagttc atcaagtgtt gctagtgctg aggacaaaac catagctaca aatagtaata atgtttcgaa tgaaaaatca aacagagacg acttattttt aatagccaca tctgaacaac cattatgcgc attacacaga gacgaaacat tagaatcaag atatttgcct ttaaaatatg taggcatttc ttcttgtttt agaaaagaag caggtgcaca tggaaaagat attagaggta tattgagagt tcaccaattt gataaagttg agcaattttg tatctctttg ccacaaacaa gttataaatt acatgaagaa atgataaaaa catgtgaaga attttatcaa tctttaaata ttccatataa aattgtaaat atcgtatcag gagctcttaa taatgctgct gccttaaaat atgatatcga aggatatttt cctactagtg ggcaatatag agaattagta tcttgtagta attgtacaga ttatcaaagt attaatttaa atattagata tacagattca aatataaaaa tatcaggaat tacaaaaagc tcaaaagata agaatgagga aaatactaaa gatgaaacaa aagatgaata tgttgatagt gaatatgaaa aatttaatca tgattttccc attgaaaata aaaattatgt tcacttgtta aatggtacta tggtagctgg tcaaagattt ttatgttgtc ttttagaaaa ctatcaaaat ggagaaggta ttattgttcc tgaaaaatta agaccataca tgaacaacac tgagtttatt ccattcatag aataa
Details for PlyoA.01238.a.B1.GE37779
HARVESTED ON: 2/6/2014
SEQUENCED ON: 2/12/2014
EXPECTED MW: 63kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 81
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV LDINLFRKEK GGNPDKIKES ERKRYHDENN VDKVIEYDDK WRKCIFELEE LKKNINMINK EIGNKKKVDK NADVEDLKKK SLNIKEEIPK YQLKEKELLK ERNKYISKIG NLLNIKVVCS DNEDNNKIVK TWGECKILPA CEENDNSIHD NVVNSNNIKR ETLNNEVDNK KKIKYYYHYD LLRKIGGANF KKGIQVAGHR GYYLTGAGFL LHNAILQYAL NFLVNKKYIP VYPPFFMKKN IMEECAELDD FEETLYKIPS TSNSTLSSQQ VSTSPTKISS QADIKDDTTC NSQKKTNIPS NEDLTRDDLF LIATSEQPLC ALHRDETLES RYLPLKYVGI SSCFRKEAGA HGKDIRGILR VHQFDKVEQF CISLPQTSYK LHEEMIKTCE EFYQSLNIPY KIVNIVSGAL NNAAALKYDI EGYFPTSGQY RELVSCSNCT DYQSINLNIR YTDSNIKISG ITKSSKDKNE ENTKDETKDE YVDSEYEKFN HDFPIENKNY VHLLNGTMVA GQRFLCCLLE NYQNGEG?IV PEKLRPYMNN TEFIPFIE
Validated NT Sequence
atggctcacc accaccacca ccatatggtt ttagatataa atttatttcg taaagaaaaa ggagggaatc ctgacaagat taaagagtct gaaaggaaac gttatcatga tgaaaataat gtagataaag ttattgaata tgatgataag tggaggaagt gtatatttga actcgaagaa ttaaaaaaaa atataaatat gataaataaa gaaataggaa ataaaaagaa ggttgacaag aatgctgatg ttgaagattt gaaaaagaag agtttaaata taaaggaaga aatacctaaa tatcaattga aagaaaaaga attattaaaa gaaagaaata agtatataag taaaatcggt aatttattaa atattaaggt agtatgttca gataatgaag ataataataa gatagttaaa acatggggtg aatgtaaaat acttccagca tgtgaagaaa atgataatag tatacatgat aatgtagtaa atagtaataa tattaaaaga gaaacattaa ataatgaagt tgataataag aaaaaaataa naatattatt atcattatga tttattaaga aaaataggtg gagctaattt taaaaaaggt atacaagtag caggtcatcg tggttattat ttaacaggtg caggtttctt attacataat gctatattac aatatgcttt aaattttttg gtwaataaaa aatatatacc tgtatatcct ccatttttta tgaaaaaaaa yattatggaa gaatgtgcmg aacttgatga ctttgaagaa acactttata aaatamcktc wwcatcwawt tcwacwttak catmycaacm srtakctaca wmtcstacwa agrtttcrtc wcarrmakmw atmaaagacg aywymwcwtg tawmtcncam aaaaaaacra myattmtkcs mattacayag agamgaanac attagwwtyt wkawattksc ctttaaaata tgtakgcatt kcttcwygtt twagaaraga agcargwgca catggaaaag atmttagagg tatattgaga gttcaccaat twgataaagt tgagcaattt kgtatctctt tgccacaaac aagttataaa ttacatgaag aaatgataaa aacatgtgaa gaattttatc aatctttaaa tattccatat aaaattgtaa atatcgtatc aggagctctt aataatgctg ctgccttaaa atatgatatc gaaggatatt ttcctactag tgggcaatat agagaattag tatcttgtag taattgtaca gattatcaaa gtattaattt aaatattaga tatacagatt caaatataaa aatatcagga attacaaaaa gctcaaaaga taagaatgag gaaaatacta aagatgaaac aaaagatgaa tatgttgata gtgaatatga aaaatttaat catgattttc ccattgaaaa taaaaattat gttcacttgt taaatggtac tatggtagct ggtcaaagat ttttatgttg tcttttagaa aactatcaaa atggagaagg taytattgtt cctgaaaaat taagaccata catgaacaac actgagttta ttccattcat agaataa
Expected Protein Sequence
MAHHHHHHMV LDINLFRTEK GGNPEKIKES EKNRFNDVSN VDKVIEYDEK WRKSIFKLEE LKKNINLINK TIGNKKKEDK NADVEDLKQK SLSMKEEIPL IQNEEKHLLK QRNMYLAKVG NILSSKVVIN NDEDHNKIVR TWGECKNYEA TDLEKSDINK EDTPNTKREV SNNNDGQNKV SIISEIKKKY YYHYDLLRKI GGVNFKKGVL VAGHRGYYLT GAGFLLNNAI MQYAINFLVN KKYTPVYPPF FMKKTIMEEC AELNDFEETL YKISSSSVAS AEDKTIATNS NNVSNEKSNR DDLFLIATSE QPLCALHRDE TLESRYLPLK YVGISSCFRK EAGAHGKDIR GILRVHQFDK VEQFCISLPQ TSYKLHEEMI KTCEEFYQSL NIPYKIVNIV SGALNNAAAL KYDIEGYFPT SGQYRELVSC SNCTDYQSIN LNIRYTDSNI KISGITKSSK DKNEENTKDE TKDEYVDSEY EKFNHDFPIE NKNYVHLLNG TMVAGQRFLC CLLENYQNGE GIIVPEKLRP YMNNTEFIPF IE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtct tggatatcaa tttatttaga actgaaaaag gaggtaaccc ggaaaagatc aaagagtcag aaaaaaacag atttaatgat gtaagtaatg tagataaagt tatagaatat gatgagaaat ggagaaagag tatatttaaa ttagaagagt taaaaaaaaa cattaaccta ataaataaaa caataggaaa taaaaaaaaa gaagataaaa atgcagatgt tgaggatttg aaacaaaaaa gtttaagcat gaaagaagaa attccattga tccaaaacga agaaaaacat ttattaaaac aaagaaatat gtatttagca aaagtaggaa atatattaag tagtaaagtt gtaattaata atgatgaaga ccataacaaa attgtacgaa catggggaga atgcaaaaat tatgaggcaa ccgatttaga aaaaagtgac ataaacaaag aagatactcc taatacaaaa agagaagtga gcaataataa tgatggacaa aataaagtca gtataataag tgaaataaaa aagaaatatt attatcatta tgatttgcta agaaaaatag ggggagtgaa ttttaagaaa ggggttttag tagctggaca tagaggttat tatcttactg gtgcaggctt tttattaaat aatgctatta tgcaatatgc tataaatttt cttgtaaata aaaaatatac acccgtatac cctccgtttt ttatgaaaaa aaccataatg gaggaatgtg cagagttaaa cgattttgaa gaaacattat ataaaataag ttcatcaagt gttgctagtg ctgaggacaa aaccatagct acaaatagta ataatgtttc gaatgaaaaa tcaaacagag acgacttatt tttaatagcc acatctgaac aaccattatg cgcattacac agagacgaaa cattagaatc aagatatttg cctttaaaat atgtaggcat ttcttcttgt tttagaaaag aagcaggtgc acatggaaaa gatattagag gtatattgag agttcaccaa tttgataaag ttgagcaatt ttgtatctct ttgccacaaa caagttataa attacatgaa gaaatgataa aaacatgtga agaattttat caatctttaa atattccata taaaattgta aatatcgtat caggagctct taataatgct gctgccttaa aatatgatat cgaaggatat tttcctacta gtgggcaata tagagaatta gtatcttgta gtaattgtac agattatcaa agtattaatt taaatattag atatacagat tcaaatataa aaatatcagg aattacaaaa agctcaaaag ataagaatga ggaaaatact aaagatgaaa caaaagatga atatgttgat agtgaatatg aaaaatttaa tcatgatttt cccattgaaa ataaaaatta tgttcacttg ttaaatggta ctatggtagc tggtcaaaga tttttatgtt gtcttttaga aaactatcaa aatggagaag gtattattgt tcctgaaaaa ttaagaccat acatgaacaa cactgagttt attccattca tagaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for PlyoA.01238.a.B11.GE42704
HARVESTED ON: 9/28/2018
SEQUENCED ON: 10/9/2018
EXPECTED MW: 63kDa
OBSERVED MW: 63kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV LDINLFRTEK GGNPEKIKES EKNRFNDVSN VDKVIEYDEK WRKSIFKLEE LKKNINLINK TIGNKKKEDK NADVEDLKQK SLSMKEEIPL IQNEEKHLLK QRNMYLAKVG NILSSKVVIN NDEDHNKIVR TWGECKNYEA TDLEKSDINK EDTPNTKREV SNNNDGQNKV SIISEIKKKY YYHYDLLRKI GGVNFKKGVL VAGHRGYYLT GAGFLLNNAI MQYAINFLVN KKYTPVYPPF FMKKTIMEEC AELNDFEETL YKISSSSVAS AEDKTIATNS NNVSNEKSNR DDLFLIATSE QPLCALHRDE TLESRYLPLK YVGISSCFRK EAGAHGKDIR GILRVHQFDK VEQFCISLPQ TSYKLHEEMI KTCEEFYQSL NIPYKIVNIV SGALNNAAAL KYDIEGYFPT SGQYRELVSC SNCTDYQSIN LNIRYTDSNI KISGITKSSK DKNEENTKDE TKDEYVDSEY EKFNHDFPIE NKNYVHLLNG TMVAGQRFLC CLLENYQNGE GIIVPEKLRP YMNNTEFIPF IE
Validated NT Sequence
atcttactca cttattctat gaatggaata aactcagtgt tgttcatgta tggtcttaat ttttcaggaa caataatacc ttctccattt tgatagtttt ctaaaagaca acataaaaat ctttgaccag ctaccatagt accatttaac aagtgaacat aatttttatt ttcaatggga aaatcatgat taaatttttc atattcacta tcaacatatt catcttttgt ttcatcttta gtattttcct cattcttatc ttttgagctt tttgtaattc ctgatatttt tatatttgaa tctgtatatc taatatttaa attaatactt tgataatctg tacaattact acaagatact aattctctat attgcccact agtaggaaaa tatccttcga tatcatattt taaggcagca gcattattaa gagctcctga tacgatattt acaattttat atggaatatt taaagattga taaaattctt cacatgtttt tatcatttct tcatgtaatt tataacttgt ttgtggcaaa gagatacaaa attgctcaac tttatcaaat tggtgaactc tcaatatacc tctaatatct tttccatgtg cacctgcttc ttttctaaaa caagaagaaa tgcctacata ttttaaaggc aaatatcttg attctaatgt ttcgtctctg tgtaatgcgc ataatggttg ttcagatgtg gctattaaaa ataagtcgtc tctgtttgat ttttcattcg aaacattatt actatttgta gctatggttt tgtcctcagc actagcaaca cttgatgaac ttattttata taatgtttct tcaaaatcgt ttaactctgc acattcctcc attatggttt ttttcataaa aaacggaggg tatacgggtg tatatttttt atttacaaga aaatttatag catattgcat aatagcatta tttaataaaa agcctgcacc agtaagataa taacctctat gtccagctac taaaacccct ttcttaaaat tcactccccc tatttttctt agcaaatcat aatgataata atatttcttt tttatttcac ttattatact gactttattt tgtccatcat tattattgct cacttctctt tttgtattag gagtatcttc tttgtttatg tcactttttt ctaaatcggt tgcctcataa tttttgcatt ctccccatgt tcgtacaatt ttgttatggt cttcatcatt attaattaca actttactac ttaatatatt tcctactttt gctaaataca tatttctttg ttttaataaa tgtttttctt cgttttggat caatggaatt tcttctttca tgcttaaact tttttgtttc aaatcctcaa catctgcatt tttatcttct ttttttttat ttcctattgt tttatttatt aggttaatgt ttttttttaa ctcttctaat ttaaatatac tctttctcca tttctcatca tattctataa ctttatctac attacttaca tcattaaatc tgtttttttc tgactctttg atcttttccg ggttacctcc tttttcagtt ctaaataaat tgatatccaa gaccatatgg tggtggtggt ggtgagccat ggtatatctc
Expected Protein Sequence
MAHHHHHHMV LDINLFRTEK GGNPEKIKES EKNRFNDVSN VDKVIEYDEK WRKSIFKLEE LKKNINLINK TIGNKKKEDK NADVEDLKQK SLSMKEEIPL IQNEEKHLLK QRNMYLAKVG NILSSKVVIN NDEDHNKIVR TWGECKNYEA TDLEKSDINK EDTPNTKREV SNNNDGQNKV SIISEIKKKY YYHYDLLRKI GGVNFKKGVL VAGHRGYYLT GAGFLLNNAI MQYAINFLVN KKYTPVYPPF FMKKTIMEEC AELNDFEETL YKISSSSVAS AEDKTIATNS NNVSNEKSNR DDLFLIATSE QPLCALHRDE TLESRYLPLK YVGISSCFRK EAGAHGKDIR GILRVHQFDK VEQFCISLPQ TSYKLHEEMI KTCEEFYQSL NIPYKIVNIV SGALNNAAAL KYDIEGYFPT SGQYRELVSC SNCTDYQSIN LNIRYTDSNI KISGITKSSK DKNEENTKDE TKDEYVDSEY EKFNHDFPIE NKNYVHLLNG TMVAGQRFLC CLLENYQNGE GIIVPEKLRP YMNNTEFIPF IE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtct tggatatcaa tttatttaga actgaaaaag gaggtaaccc ggaaaagatc aaagagtcag aaaaaaacag atttaatgat gtaagtaatg tagataaagt tatagaatat gatgagaaat ggagaaagag tatatttaaa ttagaagagt taaaaaaaaa cattaaccta ataaataaaa caataggaaa taaaaaaaaa gaagataaaa atgcagatgt tgaggatttg aaacaaaaaa gtttaagcat gaaagaagaa attccattga tccaaaacga agaaaaacat ttattaaaac aaagaaatat gtatttagca aaagtaggaa atatattaag tagtaaagtt gtaattaata atgatgaaga ccataacaaa attgtacgaa catggggaga atgcaaaaat tatgaggcaa ccgatttaga aaaaagtgac ataaacaaag aagatactcc taatacaaaa agagaagtga gcaataataa tgatggacaa aataaagtca gtataataag tgaaataaaa aagaaatatt attatcatta tgatttgcta agaaaaatag ggggagtgaa ttttaagaaa ggggttttag tagctggaca tagaggttat tatcttactg gtgcaggctt tttattaaat aatgctatta tgcaatatgc tataaatttt cttgtaaata aaaaatatac acccgtatac cctccgtttt ttatgaaaaa aaccataatg gaggaatgtg cagagttaaa cgattttgaa gaaacattat ataaaataag ttcatcaagt gttgctagtg ctgaggacaa aaccatagct acaaatagta ataatgtttc gaatgaaaaa tcaaacagag acgacttatt tttaatagcc acatctgaac aaccattatg cgcattacac agagacgaaa cattagaatc aagatatttg cctttaaaat atgtaggcat ttcttcttgt tttagaaaag aagcaggtgc acatggaaaa gatattagag gtatattgag agttcaccaa tttgataaag ttgagcaatt ttgtatctct ttgccacaaa caagttataa attacatgaa gaaatgataa aaacatgtga agaattttat caatctttaa atattccata taaaattgta aatatcgtat caggagctct taataatgct gctgccttaa aatatgatat cgaaggatat tttcctacta gtgggcaata tagagaatta gtatcttgta gtaattgtac agattatcaa agtattaatt taaatattag atatacagat tcaaatataa aaatatcagg aattacaaaa agctcaaaag ataagaatga ggaaaatact aaagatgaaa caaaagatga atatgttgat agtgaatatg aaaaatttaa tcatgatttt cccattgaaa ataaaaatta tgttcacttg ttaaatggta ctatggtagc tggtcaaaga tttttatgtt gtcttttaga aaactatcaa aatggagaag gtattattgt tcctgaaaaa ttaagaccat acatgaacaa cactgagttt attccattca tagaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for PlyoA.01238.a.B1.PW37633
PURIFICATION DATe: 1/20/2015
CONCENTRATION: 26mg/ml
OBSERVED MW: 75kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 81
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV LDINLFRKEK GGNPDKIKES ERKRYHDENN VDKVIEYDDK WRKCIFELEE LKKNINMINK EIGNKKKVDK NADVEDLKKK SLNIKEEIPK YQLKEKELLK ERNKYISKIG NLLNIKVVCS DNEDNNKIVK TWGECKILPA CEENDNSIHD NVVNSNNIKR ETLNNEVDNK KKIKYYYHYD LLRKIGGANF KKGIQVAGHR GYYLTGAGFL LHNAILQYAL NFLVNKKYIP VYPPFFMKKN IMEECAELDD FEETLYKIPS TSNSTLSSQQ VSTSPTKISS QADIKDDTTC NSQKKTNIPS NEDLTRDDLF LIATSEQPLC ALHRDETLES RYLPLKYVGI SSCFRKEAGA HGKDIRGILR VHQFDKVEQF CISLPQTSYK LHEEMIKTCE EFYQSLNIPY KIVNIVSGAL NNAAALKYDI EGYFPTSGQY RELVSCSNCT DYQSINLNIR YTDSNIKISG ITKSSKDKNE ENTKDETKDE YVDSEYEKFN HDFPIENKNY VHLLNGTMVA GQRFLCCLLE NYQNGEG?IV PEKLRPYMNN TEFIPFIE
Validated NT Sequence
atggctcacc accaccacca ccatatggtt ttagatataa atttatttcg taaagaaaaa ggagggaatc ctgacaagat taaagagtct gaaaggaaac gttatcatga tgaaaataat gtagataaag ttattgaata tgatgataag tggaggaagt gtatatttga actcgaagaa ttaaaaaaaa atataaatat gataaataaa gaaataggaa ataaaaagaa ggttgacaag aatgctgatg ttgaagattt gaaaaagaag agtttaaata taaaggaaga aatacctaaa tatcaattga aagaaaaaga attattaaaa gaaagaaata agtatataag taaaatcggt aatttattaa atattaaggt agtatgttca gataatgaag ataataataa gatagttaaa acatggggtg aatgtaaaat acttccagca tgtgaagaaa atgataatag tatacatgat aatgtagtaa atagtaataa tattaaaaga gaaacattaa ataatgaagt tgataataag aaaaaaataa naatattatt atcattatga tttattaaga aaaataggtg gagctaattt taaaaaaggt atacaagtag caggtcatcg tggttattat ttaacaggtg caggtttctt attacataat gctatattac aatatgcttt aaattttttg gtwaataaaa aatatatacc tgtatatcct ccatttttta tgaaaaaaaa yattatggaa gaatgtgcmg aacttgatga ctttgaagaa acactttata aaatamcktc wwcatcwawt tcwacwttak catmycaacm srtakctaca wmtcstacwa agrtttcrtc wcarrmakmw atmaaagacg aywymwcwtg tawmtcncam aaaaaaacra myattmtkcs mattacayag agamgaanac attagwwtyt wkawattksc ctttaaaata tgtakgcatt kcttcwygtt twagaaraga agcargwgca catggaaaag atmttagagg tatattgaga gttcaccaat twgataaagt tgagcaattt kgtatctctt tgccacaaac aagttataaa ttacatgaag aaatgataaa aacatgtgaa gaattttatc aatctttaaa tattccatat aaaattgtaa atatcgtatc aggagctctt aataatgctg ctgccttaaa atatgatatc gaaggatatt ttcctactag tgggcaatat agagaattag tatcttgtag taattgtaca gattatcaaa gtattaattt aaatattaga tatacagatt caaatataaa aatatcagga attacaaaaa gctcaaaaga taagaatgag gaaaatacta aagatgaaac aaaagatgaa tatgttgata gtgaatatga aaaatttaat catgattttc ccattgaaaa taaaaattat gttcacttgt taaatggtac tatggtagct ggtcaaagat ttttatgttg tcttttagaa aactatcaaa atggagaagg taytattgtt cctgaaaaat taagaccata catgaacaac actgagttta ttccattcat agaataa
Expressed Protein Sequence
MAHHHHHHMV LDINLFRTEK GGNPEKIKES EKNRFNDVSN VDKVIEYDEK WRKSIFKLEE LKKNINLINK TIGNKKKEDK NADVEDLKQK SLSMKEEIPL IQNEEKHLLK QRNMYLAKVG NILSSKVVIN NDEDHNKIVR TWGECKNYEA TDLEKSDINK EDTPNTKREV SNNNDGQNKV SIISEIKKKY YYHYDLLRKI GGVNFKKGVL VAGHRGYYLT GAGFLLNNAI MQYAINFLVN KKYTPVYPPF FMKKTIMEEC AELNDFEETL YKISSSSVAS AEDKTIATNS NNVSNEKSNR DDLFLIATSE QPLCALHRDE TLESRYLPLK YVGISSCFRK EAGAHGKDIR GILRVHQFDK VEQFCISLPQ TSYKLHEEMI KTCEEFYQSL NIPYKIVNIV SGALNNAAAL KYDIEGYFPT SGQYRELVSC SNCTDYQSIN LNIRYTDSNI KISGITKSSK DKNEENTKDE TKDEYVDSEY EKFNHDFPIE NKNYVHLLNG TMVAGQRFLC CLLENYQNGE GIIVPEKLRP YMNNTEFIPF IE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTCT TGGATATCAA TTTATTTAGA ACTGAAAAAG GAGGTAACCC GGAAAAGATC AAAGAGTCAG AAAAAAACAG ATTTAATGAT GTAAGTAATG TAGATAAAGT TATAGAATAT GATGAGAAAT GGAGAAAGAG TATATTTAAA TTAGAAGAGT TAAAAAAAAA CATTAACCTA ATAAATAAAA CAATAGGAAA TAAAAAAAAA GAAGATAAAA ATGCAGATGT TGAGGATTTG AAACAAAAAA GTTTAAGCAT GAAAGAAGAA ATTCCATTGA TCCAAAACGA AGAAAAACAT TTATTAAAAC AAAGAAATAT GTATTTAGCA AAAGTAGGAA ATATATTAAG TAGTAAAGTT GTAATTAATA ATGATGAAGA CCATAACAAA ATTGTACGAA CATGGGGAGA ATGCAAAAAT TATGAGGCAA CCGATTTAGA AAAAAGTGAC ATAAACAAAG AAGATACTCC TAATACAAAA AGAGAAGTGA GCAATAATAA TGATGGACAA AATAAAGTCA GTATAATAAG TGAAATAAAA AAGAAATATT ATTATCATTA TGATTTGCTA AGAAAAATAG GGGGAGTGAA TTTTAAGAAA GGGGTTTTAG TAGCTGGACA TAGAGGTTAT TATCTTACTG GTGCAGGCTT TTTATTAAAT AATGCTATTA TGCAATATGC TATAAATTTT CTTGTAAATA AAAAATATAC ACCCGTATAC CCTCCGTTTT TTATGAAAAA AACCATAATG GAGGAATGTG CAGAGTTAAA CGATTTTGAA GAAACATTAT ATAAAATAAG TTCATCAAGT GTTGCTAGTG CTGAGGACAA AACCATAGCT ACAAATAGTA ATAATGTTTC GAATGAAAAA TCAAACAGAG ACGACTTATT TTTAATAGCC ACATCTGAAC AACCATTATG CGCATTACAC AGAGACGAAA CATTAGAATC AAGATATTTG CCTTTAAAAT ATGTAGGCAT TTCTTCTTGT TTTAGAAAAG AAGCAGGTGC ACATGGAAAA GATATTAGAG GTATATTGAG AGTTCACCAA TTTGATAAAG TTGAGCAATT TTGTATCTCT TTGCCACAAA CAAGTTATAA ATTACATGAA GAAATGATAA AAACATGTGA AGAATTTTAT CAATCTTTAA ATATTCCATA TAAAATTGTA AATATCGTAT CAGGAGCTCT TAATAATGCT GCTGCCTTAA AATATGATAT CGAAGGATAT TTTCCTACTA GTGGGCAATA TAGAGAATTA GTATCTTGTA GTAATTGTAC AGATTATCAA AGTATTAATT TAAATATTAG ATATACAGAT TCAAATATAA AAATATCAGG AATTACAAAA AGCTCAAAAG ATAAGAATGA GGAAAATACT AAAGATGAAA CAAAAGATGA ATATGTTGAT AGTGAATATG AAAAATTTAA TCATGATTTT CCCATTGAAA ATAAAAATTA TGTTCACTTG TTAAATGGTA CTATGGTAGC TGGTCAAAGA TTTTTATGTT GTCTTTTAGA AAACTATCAA AATGGAGAAG GTATTATTGT TCCTGAAAAA TTAAGACCAT ACATGAACAA CACTGAGTTT ATTCCATTCA TAGAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT