SSGCID: Target Page

PlyoA.01010.a

Calcium-dependent protein kinase 4 (EC=2.7.11.1)

CENTER ID:	PlyoA.01010.a
ORGANISM:	Plasmodium yoelii yoelii
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlyoA.01010.a.B1.GE35072	full length		1	528

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PY03298
RefSeq:	XP_731293.1
UniProt:	Q7RJG2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGQEMSTQSD IQNEDQKGNK RNLKGTQGKN GLKERSTSIS KEIVKNSFNN SKLRPGMFIQ NSNVVFNEQY KGIKILGKGS FGEVILSKDK HTGHEYAIKV ISKKHVKRKT DKQSLLREVE LLKMLDHINI MKLYEFFEDN NYYYLVSDVY SGGELFDEII SRKRFYEVDA ARIIKQVLSG ITYMHKNNVV HRDLKPENIL LETKNKEDMI IKIIDFGLST HFEYSKKMKD KIGTAYYIAP DVLHGTYDEK CDIWSCGVIL YILLSGCPPF NGSNEYDILK KVETGKYTFD LPQFKKISDK AKDLIKKMLM YTSAVRISAR DALEHEWIRL MTSKDNVNID IPSLELSITN IKQFQSTQKL AQAALLYMGS KLTTIDETKE LTKIFKKMDK NGDGQLDRNE LIIGYKELLK LKGDDTTDLD NAAIEVEVDQ ILSSIDLDQN GYIEYSEFLT VAIDRKLLLS TERLEKAFKL FDKDGSGKIS ANELAQLFGL GDVSSDCWKT VLKEVDQNND GEIDFKEFRD MLIKLCNY

NT Sequence

atggggcaag aaatgtctac acagagtgat atacaaaatg aagatcaaaa gggaaacaaa agaaatttaa aggggaccca aggaaaaaat ggattgaaag aaagaagtac tagtatatca aaagaaatcg ttaaaaatag ttttaataat agtaaattaa gacctggtat gttcatacaa aatagtaatg tagtatttaa tgagcaatat aaagggatca aaattttagg aaagggttca tttggtgaag ttatattaag taaagacaaa catacaggtc atgaatatgc aattaaagta attagcaaaa agcatgttaa aagaaaaaca gataaacaaa gtctattaag agaagtagaa ttattaaaaa tgttagatca tataaatata atgaaattat atgaattttt tgaagataat aattattatt atttagtatc tgatgtatat tctggaggtg aattgtttga tgaaattatt agtaggaaac gattttatga agtagatgca gctagaataa ttaaacaagt attaagtggt attacatata tgcataaaaa taatgtagta catagagatt taaaacctga aaatattctt ctcgaaacaa aaaataaaga agatatgatt ataaaaatta ttgattttgg attatcaacc cattttgaat atagtaaaaa aatgaaagat aaaataggta ctgcctatta tattgctccg gatgttttac acggaactta tgatgaaaaa tgtgatatat ggtcatgtgg tgtcatatta tatattctct tatcaggttg tccacctttt aatgggtcta acgaatacga cattttaaaa aaggttgaga ctggaaaata tacatttgat ttacctcaat ttaaaaaaat aagtgacaaa gcgaaagatt tgataaaaaa aatgttgatg tacacaagtg ctgtgagaat atcagcaaga gatgcattag aacatgaatg gataagatta atgacaagca aagataatgt aaacattgat attccatctc ttgaattaag tattacaaat ataaaacaat ttcaaagtac acaaaagtta gcacaagccg cattattata tatgggatca aaattaacaa caatagatga aacaaaagag cttactaaaa tttttaaaaa aatggataaa aatggagatg gtcaattaga cagaaatgaa ttaataatag gatataaaga attgttaaaa ttaaaaggag atgatacaac tgatttagat aatgcagcta ttgaagttga agttgatcaa attttaagtt ctatagattt agatcaaaat ggatatatag aatattcaga atttttaaca gttgctattg atagaaaatt attattatca actgagagat tagaaaaggc gtttaaatta tttgataaag atggttcagg aaaaatatct gcaaatgaat tagctcaatt gtttggattg ggagatgtta gttctgattg ttggaaaact gttttgaagg aggttgatca gaataacgat ggagaaattg attttaaaga atttagagat atgttaataa aactatgtaa ctattaa

Details for PlyoA.01010.a.B1.GE35072

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/11/2012
EXPECTED MW:	61kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	52

Validated AA Sequence

Validated NT Sequence

cnnnnnnnnn aattttgttt nnctttaaga aggagatata ccatggctca ccaccaccac caccatatgg ggcaagaaat gtctacacag agtgatatac aaaatgaaga tcaaaaggga aacaaaagaa atttaaaggg gacccaagga aaaaatggat tgaaagaaag aagtactagt atatcaaaag aaatcgttaa aaatagtttt aataatagta aattaagacc tggtatgttc atacaaaata gtaatgtagt atttaatgag caatataaag ggatcaaaat tttaggaaag ggttcatttg gtgaagttat attaagtaaa gacaaacata caggtcatga atatgcaatt aaagtaatta gcaaaaagca tgttaaaaga aaaacagata aacaaagtct attaagagaa gtagaattat taaaaatgtt agatcatata aatataatga aattatatga attttttgaa gataataatt attattattt agtatctgat gtatattctg gaggtgaatt gtttgatgaa attattagta ggaaacgatt ttatgaagta gatgcagcta gaataattaa acaagtatta agtggtatta catatatgca taaaaataat gtagtacata gagatttaaa acctgaaaat attcttctcg aaacaaaaaa taaagaagat atgattataa aaattattga ttttggatta tcaacccatt ttgaatatag taaaaaaatg aaagataaaa taggtactgc ctattatatt gctccggatg ttttacacgg aacttatgat gaaaaatgtg atatatggtc atgtggtgtc atattatata ttctcttatc nnnnataaaa ctttcaaact aattttatta taaattatag catcgatttg ttttttaaat aattttgaga atttagataa tagtacgttg tatgcattgc gtgnggtcct gtttatactt tggaaaantg ttntcattna atgntgaatg ntaaangnta aatgntaaat gnnaaannnn nnnnnnnnnn nnnnnggnnn nccnnnnttn nnngnnnnan cnannnnntt naaaangnnn nnnncnnnnn nnnnnnnnnt tncnnnnntn naaaannann nnnnnnnnnn nattnnnnna annnnnnnnn nnnnnnna

Expected Protein Sequence

MAHHHHHHMG QEMSTQSDIQ NEDQKGNKRN LKGTQGKNGL KERSTSISKE IVKNSFNNSK LRPGMFIQNS NVVFNEQYKG IKILGKGSFG EVILSKDKHT GHEYAIKVIS KKHVKRKTDK QSLLREVELL KMLDHINIMK LYEFFEDNNY YYLVSDVYSG GELFDEIISR KRFYEVDAAR IIKQVLSGIT YMHKNNVVHR DLKPENILLE TKNKEDMIIK IIDFGLSTHF EYSKKMKDKI GTAYYIAPDV LHGTYDEKCD IWSCGVILYI LLSGCPPFNG SNEYDILKKV ETGKYTFDLP QFKKISDKAK DLIKKMLMYT SAVRISARDA LEHEWIRLMT SKDNVNIDIP SLELSITNIK QFQSTQKLAQ AALLYMGSKL TTIDETKELT KIFKKMDKNG DGQLDRNELI IGYKELLKLK GDDTTDLDNA AIEVEVDQIL SSIDLDQNGY IEYSEFLTVA IDRKLLLSTE RLEKAFKLFD KDGSGKISAN ELAQLFGLGD VSSDCWKTVL KEVDQNNDGE IDFKEFRDML IKLCNY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggggc aagaaatgtc tacacagagt gatatacaaa atgaagatca aaagggaaac aaaagaaatt taaaggggac ccaaggaaaa aatggattga aagaaagaag tactagtata tcaaaagaaa tcgttaaaaa tagttttaat aatagtaaat taagacctgg tatgttcata caaaatagta atgtagtatt taatgagcaa tataaaggga tcaaaatttt aggaaagggt tcatttggtg aagttatatt aagtaaagac aaacatacag gtcatgaata tgcaattaaa gtaattagca aaaagcatgt taaaagaaaa acagataaac aaagtctatt aagagaagta gaattattaa aaatgttaga tcatataaat ataatgaaat tatatgaatt ttttgaagat aataattatt attatttagt atctgatgta tattctggag gtgaattgtt tgatgaaatt attagtagga aacgatttta tgaagtagat gcagctagaa taattaaaca agtattaagt ggtattacat atatgcataa aaataatgta gtacatagag atttaaaacc tgaaaatatt cttctcgaaa caaaaaataa agaagatatg attataaaaa ttattgattt tggattatca acccattttg aatatagtaa aaaaatgaaa gataaaatag gtactgccta ttatattgct ccggatgttt tacacggaac ttatgatgaa aaatgtgata tatggtcatg tggtgtcata ttatatattc tcttatcagg ttgtccacct tttaatgggt ctaacgaata cgacatttta aaaaaggttg agactggaaa atatacattt gatttacctc aatttaaaaa aataagtgac aaagcgaaag atttgataaa aaaaatgttg atgtacacaa gtgctgtgag aatatcagca agagatgcat tagaacatga atggataaga ttaatgacaa gcaaagataa tgtaaacatt gatattccat ctcttgaatt aagtattaca aatataaaac aatttcaaag tacacaaaag ttagcacaag ccgcattatt atatatggga tcaaaattaa caacaataga tgaaacaaaa gagcttacta aaatttttaa aaaaatggat aaaaatggag atggtcaatt agacagaaat gaattaataa taggatataa agaattgtta aaattaaaag gagatgatac aactgattta gataatgcag ctattgaagt tgaagttgat caaattttaa gttctataga tttagatcaa aatggatata tagaatattc agaattttta acagttgcta ttgatagaaa attattatta tcaactgaga gattagaaaa ggcgtttaaa ttatttgata aagatggttc aggaaaaata tctgcaaatg aattagctca attgtttgga ttgggagatg ttagttctga ttgttggaaa actgttttga aggaggttga tcagaataac gatggagaaa ttgattttaa agaatttaga gatatgttaa taaaactatg taactattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at