PlyoA.00407.a

Adp-ribosylation factor 1

CENTER ID: PlyoA.00407.a
ORGANISM: Plasmodium yoelii yoelii
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlyoA.00407.a.B1.GE38184 full length 1 166
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PY03241
RefSeq: XP_731233.1
UniProt: Q7RJL8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYIYVKTYFI NEEKFTLKIK NDKNHDEEKN YEINFYEIGH NCSYNLIKEY ADISDDIIYI VDSIRKDKLC ECREDFIRII YDFRFIYRKC KFLIFMNKQD SNGCIKPEEI INYFALPNEL QYRCKFISSS TLSGQGLNEG LEWLLNYNVF SEKEEIIERR KRLYDY
NT Sequence
atgtatatat atgtaaaaac atattttata aatgaagaaa aatttacatt aaaaataaag aatgataaaa atcatgatga agaaaaaaat tatgaaataa atttttatga aataggacat aactgttctt acaatttaat aaaagaatat gcagacatat ccgatgatat aatttatatt gttgatagta tacgtaaaga taaattgtgt gaatgtagag aagattttat aagaataata tatgatttta gatttatata tagaaaatgt aaatttttaa tttttatgaa taaacaagat tcgaatggct gtataaagcc agaagaaatc ataaattatt ttgctttacc aaatgaatta caatatagat gtaaatttat atcctcaagt acattatctg gacaaggctt aaacgaaggt ttagaatggt tattaaatta taatgttttt tctgaaaagg aggagattat tgaaagacgc aaaaggttat atgattatta a
Details for PlyoA.00407.a.B1.GE38184
HARVESTED ON: 6/11/2014
SEQUENCED ON: 6/12/2014
EXPECTED MW: 21kDa
OBSERVED MW: 19kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMY IYVKTYFINE EKFTLKIKND KNHDEEKNYE INFYEIGHNC SYNLIKEYAD ISDDIIYIVD SIRKDKLCEC REDFIRIIYD FRFIYRKCKF LIFMNKQDSN GCIKPEEIIN YFALPNELQY RCKFISSSTL SGQGLNEGLE WLLNYNVFSE KEEIIERRKR LYDY
Validated NT Sequence
cnctagnnta attttgttta actttaagaa ggagatatac catggctcac caccaccacc accatatgta tatatatgta aaaacatatt ttataaatga agaaaaattt acattaaaaa taaagaatga taaaaatcat gatgaagaaa aaaattatga aataaatttt tatgaaatag gacataactg ttcttacaat ttaataaaag aatatgcaga catatccgat gatataattt atattgttga tagtatacgt aaagataaat tgtgtgaatg tagagaagat tttataagaa taatatatga ttttagattt atatatagaa aatgtaaatt tttaattttt atgaataaac aagattcgaa tggctgtata aagccagaag aaatcataaa ttattttgct ttaccaaatg aattacaata tagatgtaaa tttatatcct caagtacatt atctggacaa ggcttaaacg aaggtttaga atggttatta aattataatg ttttttctga aaaggaggag attattgaaa gacgcaaaag gttatatgat tattaagtga gtaagatagg atccggctgc taacaagccc cgaaagannn nttttcgggg nnnnnnnnnn cnnnctcnga agtcccgcna ncacaacnca gtaaatcgcn natnntnatg tagatcgct
Expected Protein Sequence
MAHHHHHHMY IYVKTYFINE EKFTLKIKND KNHDEEKNYE INFYEIGHNC SYNLIKEYAD ISDDIIYIVD SIRKDKLCEC REDFIRIIYD FRFIYRKCKF LIFMNKQDSN GCIKPEEIIN YFALPNELQY RCKFISSSTL SGQGLNEGLE WLLNYNVFSE KEEIIERRKR LYDY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtata tatatgtaaa aacatatttt ataaatgaag aaaaatttac attaaaaata aagaatgata aaaatcatga tgaagaaaaa aattatgaaa taaattttta tgaaatagga cataactgtt cttacaattt aataaaagaa tatgcagaca tatccgatga tataatttat attgttgata gtatacgtaa agataaattg tgtgaatgta gagaagattt tataagaata atatatgatt ttagatttat atatagaaaa tgtaaatttt taatttttat gaataaacaa gattcgaatg gctgtataaa gccagaagaa atcataaatt attttgcttt accaaatgaa ttacaatata gatgtaaatt tatatcctca agtacattat ctggacaagg cttaaacgaa ggtttagaat ggttattaaa ttataatgtt ttttctgaaa aggaggagat tattgaaaga cgcaaaaggt tatatgatta ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat