PlviB.18549.a

Phenylalanine--tRNA ligase, putative

CENTER ID: PlviB.18549.a
ORGANISM: Plasmodium vivax Salvador I
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlviB.18549.a.B1.GE37156 full length 1 495
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PVX_113350
RefSeq: XP_001616062.1
UniProt: A5K1N5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVLPLPKLIQ SNEGRVLYHI ANHPIRTVKK KIESFFKYES LDNLNSDISV RQNFDELFVP LTHPARNIKD TFYLCKNYVK YFSTLHNDLY TRLENTNGIY KYYLTTKLVG HHKILLKRTH MTAHLPDLLR RNYKNVIYTG SVYRRDEIDR FHFPIFHQTD GFLIRPDNFD VETDLKTNLQ QLIRYLFDAS DIKMKWDGNT SFPFTDPSYE LYIRGVPSGG RGTTRDDNNG GAASASLQGD PRGGEPPWVE VLGCGKIKKE VIAMALHAED IRQMIEDEIA RHDRGLLGEL HSMEAAASGA AAEGTAPNEV TPNVATPNPA TSNVAPPKGI LDKLCSAPLN NRIETQMNKF LKTIRYQGWA FGIGLERLAM LLYHIHDIRL FWSTDSRFID QFEEDVITKF HPFSNFPPVE KDISFYVSSQ FKEALFFQIC RDIASENIEQ VKKIDSYHNP RNNQTSVCYR ITYRSHSQNL THKAVNELQS KIAQMLVKQC AVTIR
NT Sequence
atggtgttgc cacttccaaa actcatccag tcgaatgaag ggagagtgtt ataccatatt gcaaaccacc ctataaggac agttaaaaaa aagatcgaaa gtttcttcaa atacgaatct ttggataacc taaacagtga catcagcgtg aggcaaaact tcgacgagct atttgtgccc ctcacacacc ccgctaggaa tataaaagat actttttact tgtgtaaaaa ttacgtaaaa tatttttcca ctctccataa tgatctgtac actcggctag aaaataccaa tggcatttat aagtactact taaccacgaa attggtgggt catcacaaaa tcctgctgaa gaggacccat atgaccgctc atttacctga cctgttaagg cgaaattaca aaaatgttat ctacacggga agtgtctaca gaagggacga aatcgatagg ttccactttc ccatttttca tcagaccgat gggtttctta tccgcccaga caactttgac gtcgaaacgg atttgaaaac gaaccttcaa cagctgattc gctacttgtt cgatgcttcg gatataaaaa tgaaatggga tggtaacacg tccttcccgt ttacagaccc gtcgtacgag ctgtacatca gaggggtacc atcaggggga agaggcacta cgcgagatga taacaacggg ggtgctgctt ctgcatcctt acagggagac cctcgaggtg gggaaccccc ctgggtggaa gtcctcgggt gcgggaaaat caaaaaggaa gtcatcgcca tggccctgca cgcggaagat atacgccaaa tgatagagga cgagattgcc cgccatgaca ggggcctcct gggcgagctc cactcgatgg aggccgccgc cagtggggca gccgcggagg ggaccgcccc taacgaggtt actcccaatg tggctacccc taacccggct acctctaacg tggccccccc caagggcatc ctggacaagc tctgcagcgc ccccctcaac aaccgcatag aaactcaaat gaacaaattc ttgaaaacga ttaggtacca agggtgggcc ttcggaatag gactcgaacg actagccatg ctgctctacc acatccacga catacgtctg ttttggagca ccgacagtag gttcatagac cagtttgagg aagacgtaat aaccaaattc caccccttca gcaacttccc tcctgtggaa aaagacatct ccttctatgt cagcagccaa tttaaagaag ctttattctt ccaaatctgc agagatatag ctagcgaaaa tatcgagcaa gtgaaaaaaa tagattccta ccacaacccc aggaataacc aaacgagcgt ctgctataga ataacctacc gctcccacag ccagaacctc acccataagg ctgtcaatga gttgcaaagt aaaattgccc aaatgttggt gaagcagtgt gcagtcacca tccggtaa
Details for PlviB.18549.a.B1.GE37156
HARVESTED ON: 7/17/2013
SEQUENCED ON: 7/20/2013
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 77
Validated AA Sequence
MAHHHHHHMV LPLPKLIQSN EGRVLYHIAN HPIRTVKKKI ESFFKYESLD NLNSDISVRQ NFDELFVPLT HPARNIKDTF YLCKNYVKYF STLHNDLYTR LENTNGIYKY YLTTKLVGHH KILLKRTHMT AHLPDLLRRN YKNVIYTGSV YRRDEIDRFH FPIFHQTDGF LIRPDNFDVE TDLKTNLQQL IRYLFDASDI KMKWDGNTSF PFTDPSYELY IRGVPSGGRG TTRDDNNGGA ASASLQGXPR GGEPPWVEVL GCGKIKKEVI AXALHAEXIR QMIEXEIARH DRGLLGELHS MEXAASGAAA EGXAPNEVTP NXXTPNPATS NXXPXGXXXS SAAXXXXXXI XXMXXXXKXX XXXXSXXXXL XXXSXXXXXX XXXXXXXXXP XX
Validated NT Sequence
ttgtnnnntt taagaaggag atataccatg gctcaccacc accaccacca tatggtgttg ccacttccaa aactcatcca gtcgaatgaa gggagagtgt tataccatat tgcaaaccac cctataagga cagttaaaaa aaagatcgaa agtttcttca aatacgaatc tttggataac ctaaacagtg acatcagcgt gaggcaaaac ttcgacgagc tatttgtgcc cctcacacac cccgctagga atataaaaga tactttttac ttgtgtaaaa attacgtaaa atatttttcc actctccata atgatctgta cactcggcta gaaaatacca atggcattta taagtactac ttaaccacga aattggtggg tcatcacaaa atcctgctga agaggaccca tatgaccgct catttacctg acctgttaag gcgaaattac aaaaatgtta tctacacggg aagtgtctac agaagggacg aaatcgatag gttccacttt cccatttttc atcagaccga tgggtttctt atccgcccag acaactttga cgtcgaaacg gatttgaaaa cgaaccttca acagctgatt cgctacttgt tcgatgcttc ggatataaaa atgaaatggg atggtaacac gtccttcccg tttacagacc cgtcgtacga gctgtacatc agaggggtac catcaggggg aagaggcact acgcgagatg ataacaacgg gggtgctgct tctgcatcct tacagggaga ncctcgaggt ggggaacccc cctgggtgga agtcctcggg tgcgggaaaa tcaaaaagga agtcatcgcc ntggccctgc acgcggaaga natacgccaa atgatagagg angagattgc ccgccatgac aggggcctcc tgggcgagct ccactcgatg gaggncgccg ccagtggggc agccgcggag gggancgccc ctaacgaggt tactcccaat gnngntaccc ctaacccggc tacntctaac gnnnnccccc nagggcancn nnnnagctct gcagcgnccn ccntnaanna cnncataaan tnaatganna nnncntnnaa annantnnnn nnnnnngnnt cnannnctan nntnctnnnn nnnnnntcnn nnnnnnnnnn ngntngnnnc nnnnnnnnnn nnnnannnnn nnnnccnnnn nnng
Expected Protein Sequence
MAHHHHHHMV LPLPKLIQSN EGRVLYHIAN HPIRTVKKKI ESFFKYESLD NLNSDISVRQ NFDELFVPLT HPARNIKDTF YLCKNYVKYF STLHNDLYTR LENTNGIYKY YLTTKLVGHH KILLKRTHMT AHLPDLLRRN YKNVIYTGSV YRRDEIDRFH FPIFHQTDGF LIRPDNFDVE TDLKTNLQQL IRYLFDASDI KMKWDGNTSF PFTDPSYELY IRGVPSGGRG TTRDDNNGGA ASASLQGDPR GGEPPWVEVL GCGKIKKEVI AMALHAEDIR QMIEDEIARH DRGLLGELHS MEAAASGAAA EGTAPNEVTP NVATPNPATS NVAPPKGILD KLCSAPLNNR IETQMNKFLK TIRYQGWAFG IGLERLAMLL YHIHDIRLFW STDSRFIDQF EEDVITKFHP FSNFPPVEKD ISFYVSSQFK EALFFQICRD IASENIEQVK KIDSYHNPRN NQTSVCYRIT YRSHSQNLTH KAVNELQSKI AQMLVKQCAV TIR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgt tgccacttcc aaaactcatc cagtcgaatg aagggagagt gttataccat attgcaaacc accctataag gacagttaaa aaaaagatcg aaagtttctt caaatacgaa tctttggata acctaaacag tgacatcagc gtgaggcaaa acttcgacga gctatttgtg cccctcacac accccgctag gaatataaaa gatacttttt acttgtgtaa aaattacgta aaatattttt ccactctcca taatgatctg tacactcggc tagaaaatac caatggcatt tataagtact acttaaccac gaaattggtg ggtcatcaca aaatcctgct gaagaggacc catatgaccg ctcatttacc tgacctgtta aggcgaaatt acaaaaatgt tatctacacg ggaagtgtct acagaaggga cgaaatcgat aggttccact ttcccatttt tcatcagacc gatgggtttc ttatccgccc agacaacttt gacgtcgaaa cggatttgaa aacgaacctt caacagctga ttcgctactt gttcgatgct tcggatataa aaatgaaatg ggatggtaac acgtccttcc cgtttacaga cccgtcgtac gagctgtaca tcagaggggt accatcaggg ggaagaggca ctacgcgaga tgataacaac gggggtgctg cttctgcatc cttacaggga gaccctcgag gtggggaacc cccctgggtg gaagtcctcg ggtgcgggaa aatcaaaaag gaagtcatcg ccatggccct gcacgcggaa gatatacgcc aaatgataga ggacgagatt gcccgccatg acaggggcct cctgggcgag ctccactcga tggaggccgc cgccagtggg gcagccgcgg aggggaccgc ccctaacgag gttactccca atgtggctac ccctaacccg gctacctcta acgtggcccc ccccaagggc atcctggaca agctctgcag cgcccccctc aacaaccgca tagaaactca aatgaacaaa ttcttgaaaa cgattaggta ccaagggtgg gccttcggaa taggactcga acgactagcc atgctgctct accacatcca cgacatacgt ctgttttgga gcaccgacag taggttcata gaccagtttg aggaagacgt aataaccaaa ttccacccct tcagcaactt ccctcctgtg gaaaaagaca tctccttcta tgtcagcagc caatttaaag aagctttatt cttccaaatc tgcagagata tagctagcga aaatatcgag caagtgaaaa aaatagattc ctaccacaac cccaggaata accaaacgag cgtctgctat agaataacct accgctccca cagccagaac ctcacccata aggctgtcaa tgagttgcaa agtaaaattg cccaaatgtt ggtgaagcag tgtgcagtca ccatccggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat