SSGCID: Target Page

PlviB.18471.a

Putative uncharacterized protein

CENTER ID:	PlviB.18471.a
ORGANISM:	Plasmodium vivax Salvador I
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlviB.18471.a.B1.GE37151	full length		1	229

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PVX_119755
RefSeq:	XP_001613147.1
UniProt:	A5KBE2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLANLRFLEL QKLLLNRFQI FKNEEITVLK KGSMKTILYE WAEFLTKEQR ATNMTHIPDE VLQSKRVADI LRDDVECRWL VGRINVGAVS GVSGVSPPSP LSGAHLARDD AALSKVDQLH LDEYPDLFHY EYPQFPYDYY DKKKMSNFYS LMKFPYNDIL NISKRIKREV SPPGGDAKEG EMRQMEEPRG GALESAGVDV VDDDFVSYSS NSYVYAPSEM ETSKENYHE

NT Sequence

atgctggcca acctgcgctt cctggaatta caaaagttgc tcctaaacag gtttcaaatt tttaagaatg aagaaataac tgtgctcaag aagggcagca tgaagaccat tttgtacgag tgggctgagt tcttaaccaa ggagcagcga gcgaccaaca tgacccacat cccagatgag gtgttgcaga gtaaaagggt ggccgacata ttgagggacg acgttgaatg caggtggttg gttggccgga tcaacgtggg cgctgttagc ggcgttagtg gggttagccc tcctagcccc cttagcggtg ctcacctcgc gcgcgacgac gccgctctca gcaaagtgga ccaactgcac ttggacgagt acccagattt gttccattac gagtacccgc agttcccgta cgactattat gacaaaaaga aaatgagcaa cttttactcc ctcatgaagt tcccctacaa tgacatccta aacatttcga agaggatcaa gagggaggtc agccccccgg gtggtgatgc taaggagggg gagatgaggc agatggagga gccgcgcggg ggagcgttgg aatccgcggg cgtggacgta gttgacgacg acttcgtgag ctactccagt aacagctatg tgtatgcccc ctcggagatg gagacgtcca aggagaacta ccacgagtag

Details for PlviB.18471.a.B1.GE37151

HARVESTED ON:	7/17/2013
SEQUENCED ON:	7/20/2013
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML ANLRFLELQK LLLNRFQIFK NEEITVLKKG SMKTILYEWA EFLTKEQRAT NMTHIPDEVL QSKRVADILR DDVECRWLVG RINVGAVSAV SGVSPPSPVS GAHLARDDAA LSKVDQLHLD EYPDLFLYEY PQFPYDYYEK KKMSNFYSLM KFPYNDILNI SKRIKREVSP PGGDAKEGEM RQMEEPRGGA LESAGVDVVD DDFVSYSSNS YVYAPSEMET SKENYHE

Validated NT Sequence

atcttactca cttactcgtg gtagttctcc ttggacgtct ccatctccga gggggcatac acatagctgt tactggagta gctcacgaag tcgtcgtcaa ctacgtccac gcccgcggat tccaacgctc ccccgcgcgg ctcctccatc tgcctcatct ccccctcctt agcatcacca cccggggggc tgacctccct cttgatcctc ttcgaaatgt ttaggatgtc attgtagggg aacttcatga gggagtaaaa gttgctcatt ttcttttttt cataatagtc gtacgggaac tgcgggtact cgtaaaggaa caaatctggg tactcgtcca agtgcagttg gtccactttg ctgagagcgg cgtcgtcgcg cgcgaggtga gcaccgctaa cggggctagg agggctaacc ccactaacgg cgctaacagc gcccacgttg atccggccaa ccaaccacct gcattcaacg tcgtccctca atatgtcggc caccctttta ctctgcaaca cctcatctgg gatgtgggtc atgttggtcg ctcgctgctc cttggttaag aactcagccc actcgtacaa aatggtcttc atgctgccct tcttgagcac agttatttct tcattcttaa aaatttgaaa cctgtttagg agcaactttt gtaattccag gaagcgcagg ttggccagca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHML ANLRFLELQK LLLNRFQIFK NEEITVLKKG SMKTILYEWA EFLTKEQRAT NMTHIPDEVL QSKRVADILR DDVECRWLVG RINVGAVSGV SGVSPPSPLS GAHLARDDAA LSKVDQLHLD EYPDLFHYEY PQFPYDYYDK KKMSNFYSLM KFPYNDILNI SKRIKREVSP PGGDAKEGEM RQMEEPRGGA LESAGVDVVD DDFVSYSSNS YVYAPSEMET SKENYHE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgg ccaacctgcg cttcctggaa ttacaaaagt tgctcctaaa caggtttcaa atttttaaga atgaagaaat aactgtgctc aagaagggca gcatgaagac cattttgtac gagtgggctg agttcttaac caaggagcag cgagcgacca acatgaccca catcccagat gaggtgttgc agagtaaaag ggtggccgac atattgaggg acgacgttga atgcaggtgg ttggttggcc ggatcaacgt gggcgctgtt agcggcgtta gtggggttag ccctcctagc ccccttagcg gtgctcacct cgcgcgcgac gacgccgctc tcagcaaagt ggaccaactg cacttggacg agtacccaga tttgttccat tacgagtacc cgcagttccc gtacgactat tatgacaaaa agaaaatgag caacttttac tccctcatga agttccccta caatgacatc ctaaacattt cgaagaggat caagagggag gtcagccccc cgggtggtga tgctaaggag ggggagatga ggcagatgga ggagccgcgc gggggagcgt tggaatccgc gggcgtggac gtagttgacg acgacttcgt gagctactcc agtaacagct atgtgtatgc cccctcggag atggagacgt ccaaggagaa ctaccacgag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat