SSGCID: Target Page

PlviB.18426.a

Putative uncharacterized protein

CENTER ID:	PlviB.18426.a
ORGANISM:	Plasmodium vivax Salvador I
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlviB.18426.a.B1.GE37144	full length		1	288

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlviB.18426.a.B1.PW37374	full length		1	288

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PVX_123505
RefSeq:	XP_001617262.1
UniProt:	A5JZX4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKNFAFPILN ISSLFILYLS SQSGLYNCMN TGKTASSAKR SNENSGVNDN SLSTEWSEKG YNFMDLVKNG YLKNEGDLDE VKDYLADELA SKIKDKLSDF LKDEKIFYDL ENMDDQDKKD LKNYLKDMSE YIGLKAADIL NSNIEESLKP LLSNSSFASL KEAMGSTSAV DLKIDDEDDE TKEEKAKEEK AKEVKAKKAK AKKAKAKKGD SKKEDSKKEG SKDENAKDEN AKDDNSSDGN SSDDNADDMD VDEATEDFIN ELVDVYEENQ QKDLEEYAQE IKTHNNLD

NT Sequence

atgaagaact ttgcatttcc catattaaac atttcgagcc tatttattct gtacttgagt tcgcaaagcg ggctgtacaa ctgcatgaac acagggaaga cagcaagtag tgcgaagcgt agtaacgaaa attccggtgt taatgataat agcctctcga ctgaatggag cgaaaagggt tacaatttta tggacttagt aaaaaatggc tacttaaaaa atgagggaga tttagatgaa gtgaaggatt acctagcgga tgagttggct agcaaaataa aggacaaatt aagtgacttt ttaaaagacg aaaagatttt ttacgattta gaaaacatgg acgatcaaga taaaaaggat ttaaaaaatt acttgaagga catgtctgaa tatattggct tgaaggctgc cgatatatta aacagcaata tagaggaatc ccttaaaccc cttttgtcca acagttcctt cgccagttta aaggaagcca tggggagtac cagcgctgtt gacttaaaaa ttgacgacga ggatgatgag acaaaggagg aaaaggcaaa ggaggaaaag gcaaaggagg taaaggcgaa gaaggcaaag gcgaagaagg caaaggcaaa gaagggagat tccaaaaagg aagattccaa aaaggaaggt tccaaagatg aaaatgccaa agacgaaaat gccaaagacg acaattccag tgacggcaat tccagtgatg acaatgccga tgacatggac gtcgatgaag cgaccgaaga tttcattaac gagttggtgg acgtatatga agaaaaccag caaaaagact tggaagagta cgcgcaagaa ataaaaaccc acaacaacct agattaa

Details for PlviB.18426.a.B1.GE37144

HARVESTED ON:	7/17/2013
SEQUENCED ON:	7/20/2013
EXPECTED MW:	33kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	101

Validated AA Sequence

MAHHHHHHMK NFAFPILNIS SLFILYLSSQ SGLYNCMNTG KTASSAKRSN ENSGVNDNSL STEWSEKGYN FMDLVKNGYL KNEGDLDEVK DYLADELASK IKDKLSDFLK DEKIFYDLEN MDDQDKKDLK NYLKDMSEYI GLKAADILNS NIEESLKPLL SNSSFASLKE AMGSTSAVDL KIDDEDDETK EEKAKEEKAK EVKAKKAKAK KAKAKKAKAK KGDSKKEDSK KEGSKDENAK DENAKDDNSS DGNSSDDNAD DMDVDEATED FINELVDVYE ENQQKDLEEY AQEIKTHNNL D

Validated NT Sequence

tcttactcac ttaatctagg ttgttgtggg tttttatttc ttgcgcgtac tcttccaagt ctttttgctg gttttcttca tatacgtcca ccaactcgtt aatgaaatct tcggtcgctt catcgacgtc catgtcatcg gcattgtcat cactggaatt gccgtcactg gaattgtcgt ctttggcatt ttcgtctttg gcattttcat ctttggaacc ttcctttttg gaatcttcct ttttggaatc tcccttcttt gcctttgcct tctttgcctt tgccttcttc gcctttgcct tcttcgcctt tacctccttt gccttttcct cctttgcctt ttcctccttt gtctcatcat cctcgtcgtc aatttttaag tcaacagcgc tggtactccc catggcttcc tttaaactgg cgaaggaact gttggacaaa aggggtttaa gggattcctc tatattgctg tttaatatat cggcagcctt caagccaata tattcagaca tgtccttcaa gtaatttttt aaatcctttt tatcttgatc gtccatgttt tctaaatcgt aaaaaatctt ttcgtctttt aaaaagtcac ttaatttgtc ctttattttg ctagccaact catccgctag gtaatccttc acttcatcta aatctccctc attttttaag tagccatttt ttactaagtc cataaaattg taaccctttt cgctccattc agtcgagagg ctattatcat taacaccgga attttcgtta ctacgcttcg cactacttgc tgtcttccct gtgttcatgc agttgtacag cccgctttgc gaactcaagt acagaataaa taggctcgaa atgtttaata tgggaaatgc aaagttcttc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHMK NFAFPILNIS SLFILYLSSQ SGLYNCMNTG KTASSAKRSN ENSGVNDNSL STEWSEKGYN FMDLVKNGYL KNEGDLDEVK DYLADELASK IKDKLSDFLK DEKIFYDLEN MDDQDKKDLK NYLKDMSEYI GLKAADILNS NIEESLKPLL SNSSFASLKE AMGSTSAVDL KIDDEDDETK EEKAKEEKAK EVKAKKAKAK KAKAKKGDSK KEDSKKEGSK DENAKDENAK DDNSSDGNSS DDNADDMDVD EATEDFINEL VDVYEENQQK DLEEYAQEIK THNNLD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga actttgcatt tcccatatta aacatttcga gcctatttat tctgtacttg agttcgcaaa gcgggctgta caactgcatg aacacaggga agacagcaag tagtgcgaag cgtagtaacg aaaattccgg tgttaatgat aatagcctct cgactgaatg gagcgaaaag ggttacaatt ttatggactt agtaaaaaat ggctacttaa aaaatgaggg agatttagat gaagtgaagg attacctagc ggatgagttg gctagcaaaa taaaggacaa attaagtgac tttttaaaag acgaaaagat tttttacgat ttagaaaaca tggacgatca agataaaaag gatttaaaaa attacttgaa ggacatgtct gaatatattg gcttgaaggc tgccgatata ttaaacagca atatagagga atcccttaaa ccccttttgt ccaacagttc cttcgccagt ttaaaggaag ccatggggag taccagcgct gttgacttaa aaattgacga cgaggatgat gagacaaagg aggaaaaggc aaaggaggaa aaggcaaagg aggtaaaggc gaagaaggca aaggcgaaga aggcaaaggc aaagaaggga gattccaaaa aggaagattc caaaaaggaa ggttccaaag atgaaaatgc caaagacgaa aatgccaaag acgacaattc cagtgacggc aattccagtg atgacaatgc cgatgacatg gacgtcgatg aagcgaccga agatttcatt aacgagttgg tggacgtata tgaagaaaac cagcaaaaag acttggaaga gtacgcgcaa gaaataaaaa cccacaacaa cctagattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for PlviB.18426.a.B1.PW37374

PURIFICATION DATe:	10/9/2013
CONCENTRATION:	17.7mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	101

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMK NFAFPILNIS SLFILYLSSQ SGLYNCMNTG KTASSAKRSN ENSGVNDNSL STEWSEKGYN FMDLVKNGYL KNEGDLDEVK DYLADELASK IKDKLSDFLK DEKIFYDLEN MDDQDKKDLK NYLKDMSEYI GLKAADILNS NIEESLKPLL SNSSFASLKE AMGSTSAVDL KIDDEDDETK EEKAKEEKAK EVKAKKAKAK KAKAKKGDSK KEDSKKEGSK DENAKDENAK DDNSSDGNSS DDNADDMDVD EATEDFINEL VDVYEENQQK DLEEYAQEIK THNNLD

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGA ACTTTGCATT TCCCATATTA AACATTTCGA GCCTATTTAT TCTGTACTTG AGTTCGCAAA GCGGGCTGTA CAACTGCATG AACACAGGGA AGACAGCAAG TAGTGCGAAG CGTAGTAACG AAAATTCCGG TGTTAATGAT AATAGCCTCT CGACTGAATG GAGCGAAAAG GGTTACAATT TTATGGACTT AGTAAAAAAT GGCTACTTAA AAAATGAGGG AGATTTAGAT GAAGTGAAGG ATTACCTAGC GGATGAGTTG GCTAGCAAAA TAAAGGACAA ATTAAGTGAC TTTTTAAAAG ACGAAAAGAT TTTTTACGAT TTAGAAAACA TGGACGATCA AGATAAAAAG GATTTAAAAA ATTACTTGAA GGACATGTCT GAATATATTG GCTTGAAGGC TGCCGATATA TTAAACAGCA ATATAGAGGA ATCCCTTAAA CCCCTTTTGT CCAACAGTTC CTTCGCCAGT TTAAAGGAAG CCATGGGGAG TACCAGCGCT GTTGACTTAA AAATTGACGA CGAGGATGAT GAGACAAAGG AGGAAAAGGC AAAGGAGGAA AAGGCAAAGG AGGTAAAGGC GAAGAAGGCA AAGGCGAAGA AGGCAAAGGC AAAGAAGGGA GATTCCAAAA AGGAAGATTC CAAAAAGGAA GGTTCCAAAG ATGAAAATGC CAAAGACGAA AATGCCAAAG ACGACAATTC CAGTGACGGC AATTCCAGTG ATGACAATGC CGATGACATG GACGTCGATG AAGCGACCGA AGATTTCATT AACGAGTTGG TGGACGTATA TGAAGAAAAC CAGCAAAAAG ACTTGGAAGA GTACGCGCAA GAAATAAAAA CCCACAACAA CCTAGATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT