PlviB.17789.a

Plasmepsin X (PM10, PMX); aspartyl protease, putative

CENTER ID: PlviB.17789.a
ORGANISM: Plasmodium vivax Salvador I
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlviB.17789.a.HE11.PD38306 full length 28 545
PlviB.17789.a.HE11.PD38307 full length 28 545
PlviB.17789.a.HE11.PD38308 full length 28 545
PlviB.17789.a.HE11.PD38320 full length 28 545

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PVX_088125
UniProt: A5KAC3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKHMGGFRTL FCGALFLLQF WGEATCHKVY KVGSEPIPCS QCKDVRECSA CLFEEQDSPH AIHLKLNKKK PSDHSNLKKH HDSLKLGGVK YYVKRGEGIS GSLGNPSGHT LDDIDSINEE IRIRREESAG VGRNGAEVTA YGGSGPSDRF AGVQRHAHAG GVSGDMASGE QRGSDEIAKG ESFIDVKNAH AVVEQTEENV FLIPLKHLRD SQFVGTLLVG VPPQEIHPIF DTGSTNLWVV TTDCEEESCK KVKRYNPYKS KTFRRSFIGK NLHIVFGSGS ISGSIGKETF VLGDHTVRNQ TFGLVESESN DSLNGDNIFD YIDFEGIVGL GFPEMLSAGK VSFFDNLLSQ NKNLSPQFSF YISPEDNTST FLVGGVSKSF YEGSIYMLPV VKEYYWEVEL DGIYVGEKKI CCEEKSYAIF DTGTSYNTMP SAQMKGFFDV VPSAPCTEEN YQEVLKNYPV IKYLFGDLVI ELLPEEYMIL NEESCIPAYM QIDVPSEKNH AYLLGSIAFM RHYYTVFVRG AGGQPSMVGV AKARAAAEAA QKVAE
NT Sequence
atgaagcaca tgggggggtt caggacgctc ttctgtggag ctctattttt gctccaattc tggggggagg ccacgtgcca taaggtatac aaggtgggtt cagaacccat cccctgttcg cagtgcaagg acgtacgtga atgcagcgcg tgtctgtttg aggagcagga ttccccacat gcgatccact tgaagctgaa taagaagaag ccaagtgacc acagcaattt gaagaagcac cacgattcgc tgaagctcgg cggggtgaag tactacgtga agaggggaga aggaatttca ggcagtctgg gtaacccctc ggggcacacc ctggatgata tagacagcat taacgaggag atcaggatca ggcgggagga gagcgccggc gtgggcagga acggcgcaga ggttaccgct tatgggggga gtggcccgtc cgaccgcttc gcgggggtgc agaggcacgc gcacgcgggg ggggtaagcg gcgacatggc gagcggcgaa cagaggggaa gcgacgaaat tgcgaagggc gagagcttca tcgacgtgaa gaacgcccac gccgtggtgg agcagacgga agagaacgtc ttcctaatcc ccctgaagca cctgcgtgat agccagttcg tggggactct cctcgtgggg gtcccccctc aagagatcca ccccatcttt gacacgggca gcacaaatct gtgggtggtt acaacagact gtgaggagga gtcttgcaaa aaggttaaga ggtacaaccc gtataagtcc aaaacgttta ggagatcatt catagggaag aacctccaca tcgtattcgg atcgggttct atttctgggt ccattgggaa ggaaacgttc gtcttaggag accatacagt gcgcaaccag acgtttgggt tagtggagag tgaatcaaac gacagcctaa atggagacaa tatatttgac tacatcgatt ttgagggcat tgtagggttg gggtttccag aaatgctatc agcagggaag gtctcttttt ttgataatct actgagtcag aataagaact tatctccgca gttttccttt tacatatctc cggaggacaa cacgtctacc ttcctagtcg gtggggtgag caagtctttt tacgagggca gcatctacat gcttcccgtg gtgaaggagt actactggga ggtggagttg gatggcattt acgtggggga gaaaaaaatt tgttgtgagg agaagagcta cgccattttt gacacgggca cttcgtataa caccatgcct agtgctcaga tgaaggggtt ttttgatgtg gttccttctg ccccctgcac ggaggagaac taccaggagg tgctgaagaa ctaccccgtg attaagtacc tgtttggcga cttggtaata gagctgctgc cagaggaata catgattttg aatgaggaga gctgcatccc ggcgtacatg caaattgacg tgccctcgga gaagaaccac gcctacctgc tcggcagcat tgccttcatg cggcactact acacggtctt cgtgcgcggc gcgggcgggc agccctccat ggtcggcgtc gcaaaggcca gagcggcggc ggaggcggcg cagaaggtgg ccgagtga
Details for PlviB.17789.a.HE11.PD38306
PURIFICATION DATe: 3/21/2018
CONCENTRATION: 5.15mg/ml
OBSERVED MW: 61kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25mM Tris pH 8.0, 200mM NaCl, 1mM TCEP, 1% Glycerol
EXPRESSION HOST: HEK293-6E
VIAL COUNT (approx.): 10
VIAL VOLUME: 100µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
KVYKVGSEPI PCSQCKDVRE CSACLFEEQD SPHAIHLKLN KKKPSDHSNL KKHHDSLKLG GVKYYVKRGE GISGSLGNPS GHTLDDIDSI NEEIRIRREE SAGVGRNGAE VTAYGGSGPS DRFAGVQRHA HAGGVSGDMA SGEQRGSDEI AKGESFIDVK NAHAVVEQTE ENVFLIPLKH LRDSQFVGTL LVGVPPQEIH PIFDTGSTNL WVVTTDCEEE SCKKVKRYNP YKSKTFRRSF IGKNLHIVFG SGSISGSIGK ETFVLGDHTV RNQTFGLVES ESNDSLNGDN IFDYIDFEGI VGLGFPEMLS AGKVSFFDNL LSQNKNLSPQ FSFYISPEDN TSTFLVGGVS KSFYEGSIYM LPVVKEYYWE VELDGIYVGE KKICCEEKSY AIFDTGTSYN TMPSAQMKGF FDVVPSAPCT EENYQEVLKN YPVIKYLFGD LVIELLPEEY MILNEESCIP AYMQIDVPSE KNHAYLLGSI AFMRHYYTVF VRGAGGQPSM VGVAKARAAA EAAQKVAEEN LYFQGSHHHH HHHH
Full NT Sequence (Expression Vector + Insert)
GTACATTTAT ATTGGCTCAT GTCCAATATG ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCCTCA CTCTCTTCCG CATCGCTGTC TGCGAGGGCC AGCTGTTGGG CTCGCGGTTG AGGACAAACT CTTCGCGGTC TTTCCAGTAC TCTTGGATCG GAAACCCGTC GGCCTCCGAA CGGTACTCCG CCACCGAGGG ACCTGAGCGA GTCCGCATCG ACCGGATCGG AAAACCTCTC GAGAAAGGCG TCTAACCAGT CACAGTCGCA AGGTAGGCTG AGCACCGTGG CGGGCGGCAG CGGGTGGCGG TCGGGGTTGT TTCTGGCGGA GGTGCTGCTG ATGATGTAAT TAAAGTAGGC GGTCTTGAGA CGGCGGATGG TCGAGGTGAG GTGTGGCAGG CTTGAGATCC AGCTGTTGGG GTGAGTACTC CCTCTCAAAA GCGGGCATTA CTTCTGCGCT AAGATTGTCA GTTTCCAAAA ACGAGGAGGA TTTGATATTC ACCTGGCCCG ATCTGGCCAT ACACTTGAGT GACAATGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC ACTCCCAGGT CCAAGTTTAA ACGGATCTCT AGCGAATTCC CTCTAGAGGA TCCATGGAAT GGTCCTGGGT GTTCCTGTTC TTCCTGAGCG TGACAACCGG CGTGCACAGC AAGGTGTACA AAGTGGGCAG CGAGCCCATT CCTTGCAGCC AGTGTAAAGA CGTGCGCGAG TGCAGCGCCT GCCTGTTCGA AGAACAAGAT AGCCCTCACG CCATCCACCT GAAGCTGAAC AAGAAGAAGC CCAGCGACCA CAGCAACCTG AAGAAGCACC ACGATTCTCT GAAGCTCGGC GGCGTGAAGT ACTACGTGAA GAGAGGCGAG GGCATCAGCG GCAGCCTTGG AAATCCTTCT GGCCACACAC TGGACGACAT CGACAGCATC AACGAGGAAA TCCGGATCAG ACGGGAAGAG TCTGCCGGCG TTGGAAGAAA TGGCGCCGAA GTGACAGCCT ATGGCGGCTC TGGCCCTTCT GATAGATTTG CCGGGGTGCA GAGACATGCC CATGCTGGCG GAGTTTCTGG CGATATGGCT TCTGGCGAGC AGAGAGGATC TGACGAGATT GCCAAGGGCG AGAGCTTCAT CGACGTGAAG AATGCCCACG CCGTGGTGGA ACAGACCGAG GAAAACGTGT TCCTGATTCC ACTGAAGCAC CTGAGAGACA GCCAGTTCGT GGGAACACTG CTTGTGGGAG TGCCTCCTCA AGAGATTCAC CCCATCTTCG ACACCGGCAG CACCAATCTG TGGGTCGTGA CCACCGATTG CGAGGAAGAG TCCTGCAAGA AAGTGAAGCG GTACAACCCC TACAAGAGCA AGACCTTCAG ACGGTCCTTC ATCGGCAAGA ACCTGCACAT CGTGTTCGGC AGCGGCTCCA TCTCTGGCAG CATCGGCAAA GAAACCTTCG TGCTGGGAGA TCACACCGTG CGGAATCAGA CCTTTGGCCT GGTGGAAAGC GAGAGCAACG ACAGCCTGAA CGGCGACAAC ATCTTCGATT ACATCGACTT TGAGGGCATC GTCGGCCTGG GCTTTCCAGA AATGCTGAGC GCCGGCAAGG TGTCCTTCTT CGACAATCTG CTGAGCCAGA ACAAGAATCT GAGCCCGCAG TTCAGCTTTT ACATCAGCCC CGAGGACAAT ACCAGCACCT TTCTCGTCGG CGGAGTGTCC AAGAGCTTCT ACGAGGGCTC TATCTACATG CTGCCCGTGG TCAAAGAGTA CTACTGGGAA GTCGAGCTGG ACGGCATCTA CGTGGGCGAG AAGAAGATTT GCTGCGAGGA AAAGAGCTAC GCCATCTTTG ATACAGGCAC CAGCTACAAC ACCATGCCTA GCGCTCAGAT GAAGGGCTTC TTTGACGTGG TGCCCAGCGC TCCCTGCACC GAAGAGAATT ACCAAGAGGT GCTGAAGAAC TACCCCGTGA TCAAGTACCT GTTCGGCGAC CTGGTTATCG AGCTGCTGCC CGAAGAGTAC ATGATCCTGA ACGAAGAGAG CTGCATCCCC GCCTACATGC AGATCGATGT GCCCTCCGAG AAGAACCACG CCTACCTGCT GGGCAGCATT GCCTTCATGA GACACTACTA CACCGTGTTC GTCAGAGGCG CTGGCGGCCA ACCTTCTATG GTTGGAGTGG CCAAAGCTAG AGCCGCCGCT GAAGCTGCTC AGAAGGTGGC CGAAGAAAAC CTGTACTTCC AAGGCAGCCA CCACCATCAT CACCATCACC ACTGATAAGC TTGCTAGCGG CCGCTCGAGG CCGGCAAGGC CAGATCCCCC GACCTCGACC TCTGGCTAAT AAAGGAAATT TATTTTCATT GCAATAGTGT GTTGGAATTT TTTGTGTCTC TCACTCGGAA GGACATATGG GAGGGCAAAT CATTTGGTCG AGATCCCTCG GAGATCTCTA GCTAGAGGAT CGATCCCCGC CCCGGACGAA CTAAACCTGA CTACGACATC TCTGCCCCTT CTTCGCGGGG CAGTGCATGT AATCCCTTCA GTTGGTTGGT ACAACTTGCC AACTGAACCC TAAACGGGTA GCATATGCTT CCCGGGTAGT AGTATATACT ATCCAGACTA ACCCTAATTC AATAGCATAT GTTACCCAAC GGGAAGCATA TGCTATCGAA TTAGGGTTAG TAAAAGGGTC CTAAGGAACA GCGATGTAGG TGGGCGGGCC AAGATAGGGG CGCGATTGCT GCGATCTGGA GGACAAATTA CACACACTTG CGCCTGAGCG CCAAGCACAG GGTTGTTGGT CCTCATATTC ACGAGGTCGC TGAGAGCACG GTGGGCTAAT GTTGCCATGG GTAGCATATA CTACCCAAAT ATCTGGATAG CATATGCTAT CCTAATCTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATTTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATCTGT ATCCGGGTAG CATATGCTAT CCTAATAGAG ATTAGGGTAG TATATGCTAT CCTAATTTAT ATCTGGGTAG CATATACTAC CCAAATATCT GGATAGCATA TGCTATCCTA ATCTATATCT GGGTAGCATA TGCTATCCTA ATCTATATCT GGGTAGCATA GGCTATCCTA ATCTATATCT GGGTAGCATA TGCTATCCTA ATCTATATCT GGGTAGTATA TGCTATCCTA ATTTATATCT GGGTAGCATA GGCTATCCTA ATCTATATCT GGGTAGCATA TGCTATCCTA ATCTATATCT GGGTAGTATA TGCTATCCTA ATCTGTATCC GGGTAGCATA TGCTATCCTC ATGATAAGCT GTCAAACATG AGAATTAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GC
Details for PlviB.17789.a.HE11.PD38307
PURIFICATION DATe: 3/21/2018
CONCENTRATION: 9.45mg/ml
OBSERVED MW: 61kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25mM Tris pH 8.0, 200mM NaCl, 1mM TCEP, 1% Glycerol
EXPRESSION HOST: HEK293-6E
VIAL COUNT (approx.): 14
VIAL VOLUME: 100µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
KVYKVGSEPI PCSQCKDVRE CSACLFEEQD SPHAIHLKLN KKKPSDHSNL KKHHDSLKLG GVKYYVKRGE GISGSLGNPS GHTLDDIDSI NEEIRIRREE SAGVGRNGAE VTAYGGSGPS DRFAGVQRHA HAGGVSGDMA SGEQRGSDEI AKGESFIDVK NAHAVVEQTE ENVFLIPLKH LRDSQFVGTL LVGVPPQEIH PIFDTGSTNL WVVTTDCEEE SCKKVKRYNP YKSKTFRRSF IGKNLHIVFG SGSISGSIGK ETFVLGDHTV RNQTFGLVES ESNDSLNGDN IFDYIDFEGI VGLGFPEMLS AGKVSFFDNL LSQNKNLSPQ FSFYISPEDN TSTFLVGGVS KSFYEGSIYM LPVVKEYYWE VELDGIYVGE KKICCEEKSY AIFDTGTSYN TMPSAQMKGF FDVVPSAPCT EENYQEVLKN YPVIKYLFGD LVIELLPEEY MILNEESCIP AYMQIDVPSE KNHAYLLGSI AFMRHYYTVF VRGAGGQPSM VGVAKARAAA EAAQKVAEEN LYFQGSHHHH HHHH
Full NT Sequence (Expression Vector + Insert)
GTACATTTAT ATTGGCTCAT GTCCAATATG ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCCTCA CTCTCTTCCG CATCGCTGTC TGCGAGGGCC AGCTGTTGGG CTCGCGGTTG AGGACAAACT CTTCGCGGTC TTTCCAGTAC TCTTGGATCG GAAACCCGTC GGCCTCCGAA CGGTACTCCG CCACCGAGGG ACCTGAGCGA GTCCGCATCG ACCGGATCGG AAAACCTCTC GAGAAAGGCG TCTAACCAGT CACAGTCGCA AGGTAGGCTG AGCACCGTGG CGGGCGGCAG CGGGTGGCGG TCGGGGTTGT TTCTGGCGGA GGTGCTGCTG ATGATGTAAT TAAAGTAGGC GGTCTTGAGA CGGCGGATGG TCGAGGTGAG GTGTGGCAGG CTTGAGATCC AGCTGTTGGG GTGAGTACTC CCTCTCAAAA GCGGGCATTA CTTCTGCGCT AAGATTGTCA GTTTCCAAAA ACGAGGAGGA TTTGATATTC ACCTGGCCCG ATCTGGCCAT ACACTTGAGT GACAATGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC ACTCCCAGGT CCAAGTTTAA ACGGATCTCT AGCGAATTCC CTCTAGAGGA TCCATGGAAT GGTCCTGGGT GTTCCTGTTC TTCCTGAGCG TGACAACCGG CGTGCACAGC AAGGTGTACA AAGTGGGCAG CGAGCCCATT CCTTGCAGCC AGTGTAAAGA CGTGCGCGAG TGCAGCGCCT GCCTGTTCGA AGAACAAGAT AGCCCTCACG CCATCCACCT GAAGCTGAAC AAGAAGAAGC CCAGCGACCA CAGCAACCTG AAGAAGCACC ACGATTCTCT GAAGCTCGGC GGCGTGAAGT ACTACGTGAA GAGAGGCGAG GGCATCAGCG GCAGCCTTGG AAATCCTTCT GGCCACACAC TGGACGACAT CGACAGCATC AACGAGGAAA TCCGGATCAG ACGGGAAGAG TCTGCCGGCG TTGGAAGAAA TGGCGCCGAA GTGACAGCCT ATGGCGGCTC TGGCCCTTCT GATAGATTTG CCGGGGTGCA GAGACATGCC CATGCTGGCG GAGTTTCTGG CGATATGGCT TCTGGCGAGC AGAGAGGATC TGACGAGATT GCCAAGGGCG AGAGCTTCAT CGACGTGAAG AATGCCCACG CCGTGGTGGA ACAGACCGAG GAAAACGTGT TCCTGATTCC ACTGAAGCAC CTGAGAGACA GCCAGTTCGT GGGAACACTG CTTGTGGGAG TGCCTCCTCA AGAGATTCAC CCCATCTTCG ACACCGGCAG CACCAATCTG TGGGTCGTGA CCACCGATTG CGAGGAAGAG TCCTGCAAGA AAGTGAAGCG GTACAACCCC TACAAGAGCA AGACCTTCAG ACGGTCCTTC ATCGGCAAGA ACCTGCACAT CGTGTTCGGC AGCGGCTCCA TCTCTGGCAG CATCGGCAAA GAAACCTTCG TGCTGGGAGA TCACACCGTG CGGAATCAGA CCTTTGGCCT GGTGGAAAGC GAGAGCAACG ACAGCCTGAA CGGCGACAAC ATCTTCGATT ACATCGACTT TGAGGGCATC GTCGGCCTGG GCTTTCCAGA AATGCTGAGC GCCGGCAAGG TGTCCTTCTT CGACAATCTG CTGAGCCAGA ACAAGAATCT GAGCCCGCAG TTCAGCTTTT ACATCAGCCC CGAGGACAAT ACCAGCACCT TTCTCGTCGG CGGAGTGTCC AAGAGCTTCT ACGAGGGCTC TATCTACATG CTGCCCGTGG TCAAAGAGTA CTACTGGGAA GTCGAGCTGG ACGGCATCTA CGTGGGCGAG AAGAAGATTT GCTGCGAGGA AAAGAGCTAC GCCATCTTTG ATACAGGCAC CAGCTACAAC ACCATGCCTA GCGCTCAGAT GAAGGGCTTC TTTGACGTGG TGCCCAGCGC TCCCTGCACC GAAGAGAATT ACCAAGAGGT GCTGAAGAAC TACCCCGTGA TCAAGTACCT GTTCGGCGAC CTGGTTATCG AGCTGCTGCC CGAAGAGTAC ATGATCCTGA ACGAAGAGAG CTGCATCCCC GCCTACATGC AGATCGATGT GCCCTCCGAG AAGAACCACG CCTACCTGCT GGGCAGCATT GCCTTCATGA GACACTACTA CACCGTGTTC GTCAGAGGCG CTGGCGGCCA ACCTTCTATG GTTGGAGTGG CCAAAGCTAG AGCCGCCGCT GAAGCTGCTC AGAAGGTGGC CGAAGAAAAC CTGTACTTCC AAGGCAGCCA CCACCATCAT CACCATCACC ACTGATAAGC TTGCTAGCGG CCGCTCGAGG CCGGCAAGGC CAGATCCCCC GACCTCGACC TCTGGCTAAT AAAGGAAATT TATTTTCATT GCAATAGTGT GTTGGAATTT TTTGTGTCTC TCACTCGGAA GGACATATGG GAGGGCAAAT CATTTGGTCG AGATCCCTCG GAGATCTCTA GCTAGAGGAT CGATCCCCGC CCCGGACGAA CTAAACCTGA CTACGACATC TCTGCCCCTT CTTCGCGGGG CAGTGCATGT AATCCCTTCA GTTGGTTGGT ACAACTTGCC AACTGAACCC TAAACGGGTA GCATATGCTT CCCGGGTAGT AGTATATACT ATCCAGACTA ACCCTAATTC AATAGCATAT GTTACCCAAC GGGAAGCATA TGCTATCGAA TTAGGGTTAG TAAAAGGGTC CTAAGGAACA GCGATGTAGG TGGGCGGGCC AAGATAGGGG CGCGATTGCT GCGATCTGGA GGACAAATTA CACACACTTG CGCCTGAGCG CCAAGCACAG GGTTGTTGGT CCTCATATTC ACGAGGTCGC TGAGAGCACG GTGGGCTAAT GTTGCCATGG GTAGCATATA CTACCCAAAT ATCTGGATAG CATATGCTAT CCTAATCTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATTTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATCTGT ATCCGGGTAG CATATGCTAT CCTAATAGAG ATTAGGGTAG TATATGCTAT CCTAATTTAT ATCTGGGTAG CATATACTAC CCAAATATCT GGATAGCATA TGCTATCCTA ATCTATATCT GGGTAGCATA TGCTATCCTA ATCTATATCT GGGTAGCATA GGCTATCCTA ATCTATATCT GGGTAGCATA TGCTATCCTA ATCTATATCT GGGTAGTATA TGCTATCCTA ATTTATATCT GGGTAGCATA GGCTATCCTA ATCTATATCT GGGTAGCATA TGCTATCCTA ATCTATATCT GGGTAGTATA TGCTATCCTA ATCTGTATCC GGGTAGCATA TGCTATCCTC ATGATAAGCT GTCAAACATG AGAATTAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GC
Details for PlviB.17789.a.HE11.PD38308
PURIFICATION DATe: 3/21/2018
CONCENTRATION: 5.02mg/ml
OBSERVED MW: 61kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25mM Tris pH 8.0, 200mM NaCl, 1mM TCEP, 1% Glycerol
EXPRESSION HOST: HEK293-6E
VIAL COUNT (approx.): 16
VIAL VOLUME: 100µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
KVYKVGSEPI PCSQCKDVRE CSACLFEEQD SPHAIHLKLN KKKPSDHSNL KKHHDSLKLG GVKYYVKRGE GISGSLGNPS GHTLDDIDSI NEEIRIRREE SAGVGRNGAE VTAYGGSGPS DRFAGVQRHA HAGGVSGDMA SGEQRGSDEI AKGESFIDVK NAHAVVEQTE ENVFLIPLKH LRDSQFVGTL LVGVPPQEIH PIFDTGSTNL WVVTTDCEEE SCKKVKRYNP YKSKTFRRSF IGKNLHIVFG SGSISGSIGK ETFVLGDHTV RNQTFGLVES ESNDSLNGDN IFDYIDFEGI VGLGFPEMLS AGKVSFFDNL LSQNKNLSPQ FSFYISPEDN TSTFLVGGVS KSFYEGSIYM LPVVKEYYWE VELDGIYVGE KKICCEEKSY AIFDTGTSYN TMPSAQMKGF FDVVPSAPCT EENYQEVLKN YPVIKYLFGD LVIELLPEEY MILNEESCIP AYMQIDVPSE KNHAYLLGSI AFMRHYYTVF VRGAGGQPSM VGVAKARAAA EAAQKVAEEN LYFQGSHHHH HHHH
Full NT Sequence (Expression Vector + Insert)
GTACATTTAT ATTGGCTCAT GTCCAATATG ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCCTCA CTCTCTTCCG CATCGCTGTC TGCGAGGGCC AGCTGTTGGG CTCGCGGTTG AGGACAAACT CTTCGCGGTC TTTCCAGTAC TCTTGGATCG GAAACCCGTC GGCCTCCGAA CGGTACTCCG CCACCGAGGG ACCTGAGCGA GTCCGCATCG ACCGGATCGG AAAACCTCTC GAGAAAGGCG TCTAACCAGT CACAGTCGCA AGGTAGGCTG AGCACCGTGG CGGGCGGCAG CGGGTGGCGG TCGGGGTTGT TTCTGGCGGA GGTGCTGCTG ATGATGTAAT TAAAGTAGGC GGTCTTGAGA CGGCGGATGG TCGAGGTGAG GTGTGGCAGG CTTGAGATCC AGCTGTTGGG GTGAGTACTC CCTCTCAAAA GCGGGCATTA CTTCTGCGCT AAGATTGTCA GTTTCCAAAA ACGAGGAGGA TTTGATATTC ACCTGGCCCG ATCTGGCCAT ACACTTGAGT GACAATGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC ACTCCCAGGT CCAAGTTTAA ACGGATCTCT AGCGAATTCC CTCTAGAGGA TCCATGGAAT GGTCCTGGGT GTTCCTGTTC TTCCTGAGCG TGACAACCGG CGTGCACAGC AAGGTGTACA AAGTGGGCAG CGAGCCCATT CCTTGCAGCC AGTGTAAAGA CGTGCGCGAG TGCAGCGCCT GCCTGTTCGA AGAACAAGAT AGCCCTCACG CCATCCACCT GAAGCTGAAC AAGAAGAAGC CCAGCGACCA CAGCAACCTG AAGAAGCACC ACGATTCTCT GAAGCTCGGC GGCGTGAAGT ACTACGTGAA GAGAGGCGAG GGCATCAGCG GCAGCCTTGG AAATCCTTCT GGCCACACAC TGGACGACAT CGACAGCATC AACGAGGAAA TCCGGATCAG ACGGGAAGAG TCTGCCGGCG TTGGAAGAAA TGGCGCCGAA GTGACAGCCT ATGGCGGCTC TGGCCCTTCT GATAGATTTG CCGGGGTGCA GAGACATGCC CATGCTGGCG GAGTTTCTGG CGATATGGCT TCTGGCGAGC AGAGAGGATC TGACGAGATT GCCAAGGGCG AGAGCTTCAT CGACGTGAAG AATGCCCACG CCGTGGTGGA ACAGACCGAG GAAAACGTGT TCCTGATTCC ACTGAAGCAC CTGAGAGACA GCCAGTTCGT GGGAACACTG CTTGTGGGAG TGCCTCCTCA AGAGATTCAC CCCATCTTCG ACACCGGCAG CACCAATCTG TGGGTCGTGA CCACCGATTG CGAGGAAGAG TCCTGCAAGA AAGTGAAGCG GTACAACCCC TACAAGAGCA AGACCTTCAG ACGGTCCTTC ATCGGCAAGA ACCTGCACAT CGTGTTCGGC AGCGGCTCCA TCTCTGGCAG CATCGGCAAA GAAACCTTCG TGCTGGGAGA TCACACCGTG CGGAATCAGA CCTTTGGCCT GGTGGAAAGC GAGAGCAACG ACAGCCTGAA CGGCGACAAC ATCTTCGATT ACATCGACTT TGAGGGCATC GTCGGCCTGG GCTTTCCAGA AATGCTGAGC GCCGGCAAGG TGTCCTTCTT CGACAATCTG CTGAGCCAGA ACAAGAATCT GAGCCCGCAG TTCAGCTTTT ACATCAGCCC CGAGGACAAT ACCAGCACCT TTCTCGTCGG CGGAGTGTCC AAGAGCTTCT ACGAGGGCTC TATCTACATG CTGCCCGTGG TCAAAGAGTA CTACTGGGAA GTCGAGCTGG ACGGCATCTA CGTGGGCGAG AAGAAGATTT GCTGCGAGGA AAAGAGCTAC GCCATCTTTG ATACAGGCAC CAGCTACAAC ACCATGCCTA GCGCTCAGAT GAAGGGCTTC TTTGACGTGG TGCCCAGCGC TCCCTGCACC GAAGAGAATT ACCAAGAGGT GCTGAAGAAC TACCCCGTGA TCAAGTACCT GTTCGGCGAC CTGGTTATCG AGCTGCTGCC CGAAGAGTAC ATGATCCTGA ACGAAGAGAG CTGCATCCCC GCCTACATGC AGATCGATGT GCCCTCCGAG AAGAACCACG CCTACCTGCT GGGCAGCATT GCCTTCATGA GACACTACTA CACCGTGTTC GTCAGAGGCG CTGGCGGCCA ACCTTCTATG GTTGGAGTGG CCAAAGCTAG AGCCGCCGCT GAAGCTGCTC AGAAGGTGGC CGAAGAAAAC CTGTACTTCC AAGGCAGCCA CCACCATCAT CACCATCACC ACTGATAAGC TTGCTAGCGG CCGCTCGAGG CCGGCAAGGC CAGATCCCCC GACCTCGACC TCTGGCTAAT AAAGGAAATT TATTTTCATT GCAATAGTGT GTTGGAATTT TTTGTGTCTC TCACTCGGAA GGACATATGG GAGGGCAAAT CATTTGGTCG AGATCCCTCG GAGATCTCTA GCTAGAGGAT CGATCCCCGC CCCGGACGAA CTAAACCTGA CTACGACATC TCTGCCCCTT CTTCGCGGGG CAGTGCATGT AATCCCTTCA GTTGGTTGGT ACAACTTGCC AACTGAACCC TAAACGGGTA GCATATGCTT CCCGGGTAGT AGTATATACT ATCCAGACTA ACCCTAATTC AATAGCATAT GTTACCCAAC GGGAAGCATA TGCTATCGAA TTAGGGTTAG TAAAAGGGTC CTAAGGAACA GCGATGTAGG TGGGCGGGCC AAGATAGGGG CGCGATTGCT GCGATCTGGA GGACAAATTA CACACACTTG CGCCTGAGCG CCAAGCACAG GGTTGTTGGT CCTCATATTC ACGAGGTCGC TGAGAGCACG GTGGGCTAAT GTTGCCATGG GTAGCATATA CTACCCAAAT ATCTGGATAG CATATGCTAT CCTAATCTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATTTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATCTGT ATCCGGGTAG CATATGCTAT CCTAATAGAG ATTAGGGTAG TATATGCTAT CCTAATTTAT ATCTGGGTAG CATATACTAC CCAAATATCT GGATAGCATA TGCTATCCTA ATCTATATCT GGGTAGCATA TGCTATCCTA ATCTATATCT GGGTAGCATA GGCTATCCTA ATCTATATCT GGGTAGCATA TGCTATCCTA ATCTATATCT GGGTAGTATA TGCTATCCTA ATTTATATCT GGGTAGCATA GGCTATCCTA ATCTATATCT GGGTAGCATA TGCTATCCTA ATCTATATCT GGGTAGTATA TGCTATCCTA ATCTGTATCC GGGTAGCATA TGCTATCCTC ATGATAAGCT GTCAAACATG AGAATTAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GC
Details for PlviB.17789.a.HE11.PD38320
PURIFICATION DATe: 6/27/2018
CONCENTRATION: 10.48mg/ml
OBSERVED MW: 61kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM Tris pH 8, 200 mM NaCl, 1 mM TCEP, 1% Glycerol
EXPRESSION HOST: HEK293-6E
VIAL COUNT (approx.): 35
VIAL VOLUME: 50µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
KVYKVGSEPI PCSQCKDVRE CSACLFEEQD SPHAIHLKLN KKKPSDHSNL KKHHDSLKLG GVKYYVKRGE GISGSLGNPS GHTLDDIDSI NEEIRIRREE SAGVGRNGAE VTAYGGSGPS DRFAGVQRHA HAGGVSGDMA SGEQRGSDEI AKGESFIDVK NAHAVVEQTE ENVFLIPLKH LRDSQFVGTL LVGVPPQEIH PIFDTGSTNL WVVTTDCEEE SCKKVKRYNP YKSKTFRRSF IGKNLHIVFG SGSISGSIGK ETFVLGDHTV RNQTFGLVES ESNDSLNGDN IFDYIDFEGI VGLGFPEMLS AGKVSFFDNL LSQNKNLSPQ FSFYISPEDN TSTFLVGGVS KSFYEGSIYM LPVVKEYYWE VELDGIYVGE KKICCEEKSY AIFDTGTSYN TMPSAQMKGF FDVVPSAPCT EENYQEVLKN YPVIKYLFGD LVIELLPEEY MILNEESCIP AYMQIDVPSE KNHAYLLGSI AFMRHYYTVF VRGAGGQPSM VGVAKARAAA EAAQKVAEEN LYFQGSHHHH HHHH
Full NT Sequence (Expression Vector + Insert)
GTACATTTAT ATTGGCTCAT GTCCAATATG ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCCTCA CTCTCTTCCG CATCGCTGTC TGCGAGGGCC AGCTGTTGGG CTCGCGGTTG AGGACAAACT CTTCGCGGTC TTTCCAGTAC TCTTGGATCG GAAACCCGTC GGCCTCCGAA CGGTACTCCG CCACCGAGGG ACCTGAGCGA GTCCGCATCG ACCGGATCGG AAAACCTCTC GAGAAAGGCG TCTAACCAGT CACAGTCGCA AGGTAGGCTG AGCACCGTGG CGGGCGGCAG CGGGTGGCGG TCGGGGTTGT TTCTGGCGGA GGTGCTGCTG ATGATGTAAT TAAAGTAGGC GGTCTTGAGA CGGCGGATGG TCGAGGTGAG GTGTGGCAGG CTTGAGATCC AGCTGTTGGG GTGAGTACTC CCTCTCAAAA GCGGGCATTA CTTCTGCGCT AAGATTGTCA GTTTCCAAAA ACGAGGAGGA TTTGATATTC ACCTGGCCCG ATCTGGCCAT ACACTTGAGT GACAATGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC ACTCCCAGGT CCAAGTTTAA ACGGATCTCT AGCGAATTCC CTCTAGAGGA TCCATGGAAT GGTCCTGGGT GTTCCTGTTC TTCCTGAGCG TGACAACCGG CGTGCACAGC AAGGTGTACA AAGTGGGCAG CGAGCCCATT CCTTGCAGCC AGTGTAAAGA CGTGCGCGAG TGCAGCGCCT GCCTGTTCGA AGAACAAGAT AGCCCTCACG CCATCCACCT GAAGCTGAAC AAGAAGAAGC CCAGCGACCA CAGCAACCTG AAGAAGCACC ACGATTCTCT GAAGCTCGGC GGCGTGAAGT ACTACGTGAA GAGAGGCGAG GGCATCAGCG GCAGCCTTGG AAATCCTTCT GGCCACACAC TGGACGACAT CGACAGCATC AACGAGGAAA TCCGGATCAG ACGGGAAGAG TCTGCCGGCG TTGGAAGAAA TGGCGCCGAA GTGACAGCCT ATGGCGGCTC TGGCCCTTCT GATAGATTTG CCGGGGTGCA GAGACATGCC CATGCTGGCG GAGTTTCTGG CGATATGGCT TCTGGCGAGC AGAGAGGATC TGACGAGATT GCCAAGGGCG AGAGCTTCAT CGACGTGAAG AATGCCCACG CCGTGGTGGA ACAGACCGAG GAAAACGTGT TCCTGATTCC ACTGAAGCAC CTGAGAGACA GCCAGTTCGT GGGAACACTG CTTGTGGGAG TGCCTCCTCA AGAGATTCAC CCCATCTTCG ACACCGGCAG CACCAATCTG TGGGTCGTGA CCACCGATTG CGAGGAAGAG TCCTGCAAGA AAGTGAAGCG GTACAACCCC TACAAGAGCA AGACCTTCAG ACGGTCCTTC ATCGGCAAGA ACCTGCACAT CGTGTTCGGC AGCGGCTCCA TCTCTGGCAG CATCGGCAAA GAAACCTTCG TGCTGGGAGA TCACACCGTG CGGAATCAGA CCTTTGGCCT GGTGGAAAGC GAGAGCAACG ACAGCCTGAA CGGCGACAAC ATCTTCGATT ACATCGACTT TGAGGGCATC GTCGGCCTGG GCTTTCCAGA AATGCTGAGC GCCGGCAAGG TGTCCTTCTT CGACAATCTG CTGAGCCAGA ACAAGAATCT GAGCCCGCAG TTCAGCTTTT ACATCAGCCC CGAGGACAAT ACCAGCACCT TTCTCGTCGG CGGAGTGTCC AAGAGCTTCT ACGAGGGCTC TATCTACATG CTGCCCGTGG TCAAAGAGTA CTACTGGGAA GTCGAGCTGG ACGGCATCTA CGTGGGCGAG AAGAAGATTT GCTGCGAGGA AAAGAGCTAC GCCATCTTTG ATACAGGCAC CAGCTACAAC ACCATGCCTA GCGCTCAGAT GAAGGGCTTC TTTGACGTGG TGCCCAGCGC TCCCTGCACC GAAGAGAATT ACCAAGAGGT GCTGAAGAAC TACCCCGTGA TCAAGTACCT GTTCGGCGAC CTGGTTATCG AGCTGCTGCC CGAAGAGTAC ATGATCCTGA ACGAAGAGAG CTGCATCCCC GCCTACATGC AGATCGATGT GCCCTCCGAG AAGAACCACG CCTACCTGCT GGGCAGCATT GCCTTCATGA GACACTACTA CACCGTGTTC GTCAGAGGCG CTGGCGGCCA ACCTTCTATG GTTGGAGTGG CCAAAGCTAG AGCCGCCGCT GAAGCTGCTC AGAAGGTGGC CGAAGAAAAC CTGTACTTCC AAGGCAGCCA CCACCATCAT CACCATCACC ACTGATAAGC TTGCTAGCGG CCGCTCGAGG CCGGCAAGGC CAGATCCCCC GACCTCGACC TCTGGCTAAT AAAGGAAATT TATTTTCATT GCAATAGTGT GTTGGAATTT TTTGTGTCTC TCACTCGGAA GGACATATGG GAGGGCAAAT CATTTGGTCG AGATCCCTCG GAGATCTCTA GCTAGAGGAT CGATCCCCGC CCCGGACGAA CTAAACCTGA CTACGACATC TCTGCCCCTT CTTCGCGGGG CAGTGCATGT AATCCCTTCA GTTGGTTGGT ACAACTTGCC AACTGAACCC TAAACGGGTA GCATATGCTT CCCGGGTAGT AGTATATACT ATCCAGACTA ACCCTAATTC AATAGCATAT GTTACCCAAC GGGAAGCATA TGCTATCGAA TTAGGGTTAG TAAAAGGGTC CTAAGGAACA GCGATGTAGG TGGGCGGGCC AAGATAGGGG CGCGATTGCT GCGATCTGGA GGACAAATTA CACACACTTG CGCCTGAGCG CCAAGCACAG GGTTGTTGGT CCTCATATTC ACGAGGTCGC TGAGAGCACG GTGGGCTAAT GTTGCCATGG GTAGCATATA CTACCCAAAT ATCTGGATAG CATATGCTAT CCTAATCTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATTTAT ATCTGGGTAG CATAGGCTAT CCTAATCTAT ATCTGGGTAG CATATGCTAT CCTAATCTAT ATCTGGGTAG TATATGCTAT CCTAATCTGT ATCCGGGTAG CATATGCTAT CCTAATAGAG ATTAGGGTAG TATATGCTAT CCTAATTTAT ATCTGGGTAG CATATACTAC CCAAATATCT GGATAGCATA TGCTATCCTA ATCTATATCT GGGTAGCATA TGCTATCCTA ATCTATATCT GGGTAGCATA GGCTATCCTA ATCTATATCT GGGTAGCATA TGCTATCCTA ATCTATATCT GGGTAGTATA TGCTATCCTA ATTTATATCT GGGTAGCATA GGCTATCCTA ATCTATATCT GGGTAGCATA TGCTATCCTA ATCTATATCT GGGTAGTATA TGCTATCCTA ATCTGTATCC GGGTAGCATA TGCTATCCTC ATGATAAGCT GTCAAACATG AGAATTAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GC