SSGCID: Target Page

PlviB.01586.a

ATP-dependent RNA helicase, putative

CENTER ID:	PlviB.01586.a
ORGANISM:	Plasmodium vivax Salvador I
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlviB.01586.a.B1.GE37141	full length		1	433

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PVX_095195
RefSeq:	XP_001614505.1
UniProt:	A5K7L9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSYRTNCAAP NALNNSNENN LNKIDDSANA PFDESWKKRI LEPLKDHRYR TEDVTKTKGN EFEDYFLKRE LLMGIFEKGY EKPSPIQEES IPVALAGKNI LARAKNGTGK TAAFAIPLLE KCNTHKNFIQ GLILVPTREL ALQTSAMIKE LGKHMKIQCM VTTGGTSLRE DIMRLYNVVH ILCGTPGRIL DLANKDVANL SGCHIMVMDE ADKLLSPEFQ PIVEELMKFL PKEKQILMYS ATFPVTVKEF RQIYLSDAHE INLMDELTLK GITQYYAFVK ERQKVHCLNT LFAKLQINQA IIFCNSITRV ELLAKKITEL GYSSFYIHAR MSQTHRNRVF HDFRNGACRC LVSSDLFTRG IDIQSVNVVI NFDFPKNSET YLHRIGRSGR YGHLGLAINL ITYEDRFNLY KIELELGTEI QPIPNEIDPS LYT

NT Sequence

atgagctata gaaccaactg cgccgctccg aacgctttga ataactccaa tgaaaacaat ttaaacaaaa tagacgacag cgcaaatgcc ccttttgacg aaagctggaa aaagagaatt ctggagcccc tcaaagacca ccgatacaga acggaagatg tcacgaagac caagggaaat gagtttgaag attacttctt aaaaagggag cttcttatgg gcatatttga aaagggctat gagaagccgt ctcccattca agaggaaagc atccccgtgg cgctggccgg caagaacatt ctagcgcggg ccaagaatgg tacagggaag acggcggcct ttgcaattcc cctcctggag aagtgcaaca cgcacaagaa tttcattcaa ggactcatcc tcgtgccgac gcgggaactc gccctgcaga cctcagccat gatcaaggag ctggggaagc acatgaagat tcagtgcatg gtcaccacgg gagggacgtc gctccgagag gacatcatgc gactctacaa cgtcgtgcac atcctatgtg gaacaccagg gagaatcctc gatttggcaa acaaagatgt agccaaccta tcagggtgcc acataatggt catggatgaa gcagataaat tattatcgcc agagtttcag cccatcgtag aagagctaat gaaattctta cccaaagaga aacaaatact aatgtactct gctaccttcc ccgtcacggt aaaggagttc agacagatct acctctcaga tgctcacgaa ataaatctga tggacgagct gaccttgaaa ggaattacac agtactatgc ctttgtaaag gaaagacaaa aggtacattg cctgaatacc ctttttgcaa agctccaaat aaatcaagcc atcatcttct gtaatagcat aactagagta gagttactag ccaaaaaaat caccgaatta ggttacagct ctttctacat acatgcaaga atgtctcaaa cccatcgaaa cagagtcttt catgactttc gaaatggagc ctgcagatgt ttagtttctt cggacctatt tacaaggggt atagacatac agtctgttaa tgtggttata aattttgatt ttccaaaaaa ctcggagacg taccttcata gaattggtcg atctgggaga tatggccacc tgggcttggc catcaacttg attacctatg aggaccgttt caatttgtac aaaatcgagt tggagctcgg caccgagatt cagcccattc cgaacgaaat cgacccctcc ctctacacct aa

Details for PlviB.01586.a.B1.GE37141

HARVESTED ON:	7/17/2013
SEQUENCED ON:	7/20/2013
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	74

Validated AA Sequence

MAHHHHHHMS YRTNCAAPNA LNNSNENNLN KIDDSANAPF DESWKKRILE PLKDHRYRTE DVTKTKGNEF EDYFLKRELL MGIFEKGYEK PSPIQEESIP VALAGKNILA RAKNGTGKTA AFAIPLLEKC NTHKNFIQGL ILVPTRELAL QTSAMIKELG KHMKIQCMVT TGGTSLREDI MRLYNVVHIL CGTPGRILDL TNKDVANLSG CHIMVMDEAD KLLSPEFQPI VEELMKFLPK EKQILMYSAT FPVTVKEFRQ IYLSXAHEIN LMDELTLKGI TQYYAFVKER QKXHCLXTLF AKLXINQAII FXNSXTRVEL LAKKSXN

Validated NT Sequence

tttgtttaac tttaagaagg aganatacca tggctcacca ccaccaccac catatgagct atagaaccaa ctgcgccgct ccgaacgctt tgaataactc caatgaaaac aatttaaaca aaatagacga cagcgctaat gccccttttg acgaaagctg gaaaaagaga attctggagc ccctcaaaga ccaccgatac agaacggaag atgtcacgaa gaccaaggga aatgagtttg aagattactt cttaaaaagg gagcttctta tgggcatatt tgaaaagggc tatgagaagc cgtctcccat tcaagaggaa agcatccccg tggcgctggc cggcaagaac attctagcgc gggccaagaa tggtacaggg aagacggcgg cctttgcaat tcccctcctg gagaagtgca acacgcacaa gaatttcatt caaggactca tcctcgtgcc gacgcgggaa ctcgccctgc agacctcagc catgatcaag gagctgggga agcacatgaa gattcagtgc atggtcacca cgggagggac gtcgctccga gaggacatca tgcgactcta caacgtcgtg cacatcctat gtggaacacc agggagaatc ctcgatttga caaacaaaga tgtagccaac ctatcagggt gccacataat ggtcatggat gaagcagata aattattatc gccagagttt cagcccatcg tagaagagct aatgaaattc ttacccaaag agaaacaaat actaatgtac tctgctacct tccccgtcac ggtaaaggag ttcagacaga tctacctctc anatgctcac gaaataaatc tgatggacga gctgaccttg aaaggaatta cacagtacta tgcctttgta aaggaaagac aaaaggnaca ttgcctgaan accctttttg caaagctccn aataaatcaa gccatcatct tctgnaatag cntaactaga gtagagttac tagcnaaaaa atcnncgaat taggttacag ctntttntac ntnnntgcnn gnatgnctcn aacccntcna annnantcnt tnntganntn gaantnnncc tgnnnatgtt nnnnnnnnnc nattnnnngg nnnannnnnn annnnnnnna nntnnnntnn nannnnnnnn nnntncnaan nnnnnnnnnn nnnnnnnnnn nnnnnnnncn nnnnnnnnnn nncnnng

Expected Protein Sequence

MAHHHHHHMS YRTNCAAPNA LNNSNENNLN KIDDSANAPF DESWKKRILE PLKDHRYRTE DVTKTKGNEF EDYFLKRELL MGIFEKGYEK PSPIQEESIP VALAGKNILA RAKNGTGKTA AFAIPLLEKC NTHKNFIQGL ILVPTRELAL QTSAMIKELG KHMKIQCMVT TGGTSLREDI MRLYNVVHIL CGTPGRILDL ANKDVANLSG CHIMVMDEAD KLLSPEFQPI VEELMKFLPK EKQILMYSAT FPVTVKEFRQ IYLSDAHEIN LMDELTLKGI TQYYAFVKER QKVHCLNTLF AKLQINQAII FCNSITRVEL LAKKITELGY SSFYIHARMS QTHRNRVFHD FRNGACRCLV SSDLFTRGID IQSVNVVINF DFPKNSETYL HRIGRSGRYG HLGLAINLIT YEDRFNLYKI ELELGTEIQP IPNEIDPSLY T

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagct atagaaccaa ctgcgccgct ccgaacgctt tgaataactc caatgaaaac aatttaaaca aaatagacga cagcgcaaat gccccttttg acgaaagctg gaaaaagaga attctggagc ccctcaaaga ccaccgatac agaacggaag atgtcacgaa gaccaaggga aatgagtttg aagattactt cttaaaaagg gagcttctta tgggcatatt tgaaaagggc tatgagaagc cgtctcccat tcaagaggaa agcatccccg tggcgctggc cggcaagaac attctagcgc gggccaagaa tggtacaggg aagacggcgg cctttgcaat tcccctcctg gagaagtgca acacgcacaa gaatttcatt caaggactca tcctcgtgcc gacgcgggaa ctcgccctgc agacctcagc catgatcaag gagctgggga agcacatgaa gattcagtgc atggtcacca cgggagggac gtcgctccga gaggacatca tgcgactcta caacgtcgtg cacatcctat gtggaacacc agggagaatc ctcgatttgg caaacaaaga tgtagccaac ctatcagggt gccacataat ggtcatggat gaagcagata aattattatc gccagagttt cagcccatcg tagaagagct aatgaaattc ttacccaaag agaaacaaat actaatgtac tctgctacct tccccgtcac ggtaaaggag ttcagacaga tctacctctc agatgctcac gaaataaatc tgatggacga gctgaccttg aaaggaatta cacagtacta tgcctttgta aaggaaagac aaaaggtaca ttgcctgaat accctttttg caaagctcca aataaatcaa gccatcatct tctgtaatag cataactaga gtagagttac tagccaaaaa aatcaccgaa ttaggttaca gctctttcta catacatgca agaatgtctc aaacccatcg aaacagagtc tttcatgact ttcgaaatgg agcctgcaga tgtttagttt cttcggacct atttacaagg ggtatagaca tacagtctgt taatgtggtt ataaattttg attttccaaa aaactcggag acgtaccttc atagaattgg tcgatctggg agatatggcc acctgggctt ggccatcaac ttgattacct atgaggaccg tttcaatttg tacaaaatcg agttggagct cggcaccgag attcagccca ttccgaacga aatcgacccc tccctctaca cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat