SSGCID: Target Page

PlviB.01489.a

Serine/threonine-protein kinase NEK4, putative

CENTER ID:	PlviB.01489.a
ORGANISM:	Plasmodium vivax Salvador I
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlviB.01489.a.B1.GE37138	full length		1	310

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlviB.01489.a.B1.PW37352	full length		1	310

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PVX_096360
RefSeq:	XP_001612826.1
UniProt:	A5KC81

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNKYEKIRDI GKGNYGNTIL VRDKKNDHYV MKIINISQMT PKEKRQCLKE VELLSKLNHP FIVKYIESYI EGDTLRIVMK HCKGGDLYHY IQNKKKQNTP IKETRILIWL TQILTALKFL HSNHILHRDM KSLNILIDND KRVRLCDFGI SKVLENTLDY ANTLIGTPYY LSPELCKDKK YSWPSDVWAT GCLIYELATF RTPFHSTKGI QQLCYNIRYA PIPDLPNIYS KELNNIYKSM LIREPSYRAT VQQLLVSDIV QRQLKLLIEE KIREKQNMKR PLKEKPAAEN EGPGANEQEV KTLLLDVVDA

NT Sequence

atgaacaaat atgaaaagat cagggacatt gggaagggga actacgggaa taccattctt gtgcgcgaca agaagaacga ccactacgtg atgaaaataa ttaacatatc tcagatgacc ccaaaggaga agaggcagtg tttgaaggag gtggagctgc tgtccaagct gaaccacccc ttcatcgtca agtacatcga gagctacata gagggggaca cgctgagaat cgtcatgaag cactgcaaag gcggagacct ctaccactac atacagaaca agaagaagca gaacaccccc ataaaggaga cgcgcatcct catctggctg actcaaattt tgacggcgct taagtttttg cactccaacc acatcctcca cagggacatg aagtctctga acatcctcat cgataacgac aagcgagtgc gtctgtgcga ctttgggatc tcgaaggtcc tcgaaaatac gcttgactac gccaacacgc tgatagggac gccctactac ttaagccccg agctgtgtaa agataaaaaa tacagctggc cctccgacgt ctgggccacg ggctgcctca tttacgagct ggccaccttc cgcacgccct tccactccac caaggggatt cagcagctct gctacaacat tagatacgcc cccatcccag accttccgaa catttactct aaggagctga ataacatcta caagagcatg ctgattcggg agcccagcta ccgagccacc gtgcagcagc tgctcgtctc cgacattgtg cagagacaac tgaagctgct gattgaggag aagatccggg agaagcaaaa catgaagagg cccctaaagg aaaagcccgc ggctgaaaac gaggggccgg gtgcaaatga gcaggaggtc aagacgctgc tcctggacgt cgtcgacgcg tga

Details for PlviB.01489.a.B1.GE37138

HARVESTED ON:	7/17/2013
SEQUENCED ON:	7/20/2013
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN KYEKIRDIGK GNYGNTILVR DKKNDHYVMK IINISQMTPK EKRQCLKEVE LLSKLSHPFI VKYIESYIEG DTLRIVMKHC KGGDLYHYIQ NKKKQNTPIK ETRILIWLTQ ILTALKFLHS NHILHRDMKS LNILIDNDKR VRLCDFGISK VLENTLDYAN TLIGTPYYLS PELCKDKKYS WPSDVWATGC LIYELATFRT PFHSTKGIQQ LCYNIRYAPI PDLPNIYSKE LNNIYKSMLI REPSYRATVQ QLLVSDIVQR QLKLLIEEKI REKQNMKRPL KEKPAAENEG PGANVQEVKT LLLDVVDA

Validated NT Sequence

tcttactcac ttacgcgtcg acgacgtcca ggagcagcgt cttgacctcc tgcacatttg cacccggccc ctcgttttca gccgcgggct tttcctttag gggcctcttc atgttttgct tctcccggat cttctcctca atcagcagct tcagttgtct ctgcacaatg tcggagacga gcagctgctg cacggtggct cggtagctgg gctcccgaat cagcatgctc ttgtagatgt tattcagctc cttagagtaa atgttcggaa ggtctgggat gggggcgtat ctaatgttgt agcagagctg ctgaatcccc ttggtggagt ggaagggcgt gcggaaggtg gccagctcgt aaatgaggca gcccgtggcc cagacgtcgg agggccagct gtatttttta tctttacaca gctcggggct taagtagtag ggcgtcccta tcagcgtgtt ggcgtagtca agcgtatttt cgaggacctt cgagatccca aagtcgcaca gacgcactcg cttgtcgtta tcgatgagga tgttcagaga cttcatgtcc ctgtggagga tgtggttgga gtgcaaaaac ttaagcgccg tcaaaatttg agtcagccag atgaggatgc gcgtctcctt tatgggggtg ttctgcttct tcttgttctg tatgtagtgg tagaggtctc cgcctttgca gtgcttcatg acgattctca gcgtgtcccc ctctatgtag ctctcgatgt acttgacgat gaaggggtgg ctcagcttgg acagcagctc cacctccttc aaacactgcc tcttctcctt tggggtcatc tgagatatgt taattatttt catcacgtag tggtcgttct tcttgtcgcg cacaagaatg gtattcccgt agttcccctt cccaatgtcc ctgatctttt catatttgtt catatggtgg tggtggtggt gagccatggt atatctcctt cttaaa

Expected Protein Sequence

MAHHHHHHMN KYEKIRDIGK GNYGNTILVR DKKNDHYVMK IINISQMTPK EKRQCLKEVE LLSKLNHPFI VKYIESYIEG DTLRIVMKHC KGGDLYHYIQ NKKKQNTPIK ETRILIWLTQ ILTALKFLHS NHILHRDMKS LNILIDNDKR VRLCDFGISK VLENTLDYAN TLIGTPYYLS PELCKDKKYS WPSDVWATGC LIYELATFRT PFHSTKGIQQ LCYNIRYAPI PDLPNIYSKE LNNIYKSMLI REPSYRATVQ QLLVSDIVQR QLKLLIEEKI REKQNMKRPL KEKPAAENEG PGANEQEVKT LLLDVVDA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca aatatgaaaa gatcagggac attgggaagg ggaactacgg gaataccatt cttgtgcgcg acaagaagaa cgaccactac gtgatgaaaa taattaacat atctcagatg accccaaagg agaagaggca gtgtttgaag gaggtggagc tgctgtccaa gctgaaccac cccttcatcg tcaagtacat cgagagctac atagaggggg acacgctgag aatcgtcatg aagcactgca aaggcggaga cctctaccac tacatacaga acaagaagaa gcagaacacc cccataaagg agacgcgcat cctcatctgg ctgactcaaa ttttgacggc gcttaagttt ttgcactcca accacatcct ccacagggac atgaagtctc tgaacatcct catcgataac gacaagcgag tgcgtctgtg cgactttggg atctcgaagg tcctcgaaaa tacgcttgac tacgccaaca cgctgatagg gacgccctac tacttaagcc ccgagctgtg taaagataaa aaatacagct ggccctccga cgtctgggcc acgggctgcc tcatttacga gctggccacc ttccgcacgc ccttccactc caccaagggg attcagcagc tctgctacaa cattagatac gcccccatcc cagaccttcc gaacatttac tctaaggagc tgaataacat ctacaagagc atgctgattc gggagcccag ctaccgagcc accgtgcagc agctgctcgt ctccgacatt gtgcagagac aactgaagct gctgattgag gagaagatcc gggagaagca aaacatgaag aggcccctaa aggaaaagcc cgcggctgaa aacgaggggc cgggtgcaaa tgagcaggag gtcaagacgc tgctcctgga cgtcgtcgac gcgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for PlviB.01489.a.B1.PW37352

PURIFICATION DATe:	8/28/2013
CONCENTRATION:	35.5mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACA AATATGAAAA GATCAGGGAC ATTGGGAAGG GGAACTACGG GAATACCATT CTTGTGCGCG ACAAGAAGAA CGACCACTAC GTGATGAAAA TAATTAACAT ATCTCAGATG ACCCCAAAGG AGAAGAGGCA GTGTTTGAAG GAGGTGGAGC TGCTGTCCAA GCTGAACCAC CCCTTCATCG TCAAGTACAT CGAGAGCTAC ATAGAGGGGG ACACGCTGAG AATCGTCATG AAGCACTGCA AAGGCGGAGA CCTCTACCAC TACATACAGA ACAAGAAGAA GCAGAACACC CCCATAAAGG AGACGCGCAT CCTCATCTGG CTGACTCAAA TTTTGACGGC GCTTAAGTTT TTGCACTCCA ACCACATCCT CCACAGGGAC ATGAAGTCTC TGAACATCCT CATCGATAAC GACAAGCGAG TGCGTCTGTG CGACTTTGGG ATCTCGAAGG TCCTCGAAAA TACGCTTGAC TACGCCAACA CGCTGATAGG GACGCCCTAC TACTTAAGCC CCGAGCTGTG TAAAGATAAA AAATACAGCT GGCCCTCCGA CGTCTGGGCC ACGGGCTGCC TCATTTACGA GCTGGCCACC TTCCGCACGC CCTTCCACTC CACCAAGGGG ATTCAGCAGC TCTGCTACAA CATTAGATAC GCCCCCATCC CAGACCTTCC GAACATTTAC TCTAAGGAGC TGAATAACAT CTACAAGAGC ATGCTGATTC GGGAGCCCAG CTACCGAGCC ACCGTGCAGC AGCTGCTCGT CTCCGACATT GTGCAGAGAC AACTGAAGCT GCTGATTGAG GAGAAGATCC GGGAGAAGCA AAACATGAAG AGGCCCCTAA AGGAAAAGCC CGCGGCTGAA AACGAGGGGC CGGGTGCAAA TGAGCAGGAG GTCAAGACGC TGCTCCTGGA CGTCGTCGAC GCGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT