SSGCID: Target Page

PlviB.01209.a

Serine/threonine-protein kinase Nek1, putative

CENTER ID:	PlviB.01209.a
ORGANISM:	Plasmodium vivax Salvador I
ASSOCIATED DISEASE:	Malaria
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PlviB.01209.a.B1.GE37136	full length		1	272

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PlasmoDB:PVX_079950
RefSeq:	XP_001613698.1
UniProt:	A5K999

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTSTSRKIG PYEVVKSIGR GSFGIVTAVK NAQGEIFVVK QLDMSCMNHK EKMNVVNELR ALIEVSVHPF IVRYKEAFLE DNILYVAMDY CSKGDLSKYI KRYKKTNTLI PERKIKRWLL QIITAIKFMH DRKLIHRDLK CNNIFLDDDE RAKVGDFGLA KIFENTEQTT TVCGTIGYMA PEICRNAAYS FPADIWSLGV ILYELMSLRH PFKSEHSNML STAQKICEEE PEPLPTSYSN DLIHLCQWML KKNSEERPTS CDIISTDYLQ VR

NT Sequence

atgaccacgt ctacatcgag gaaaataggt ccttatgagg ttgtaaaatc tatcgggcgg gggtcatttg gaattgtcac ggcggtgaag aacgcccagg gggaaatctt cgtcgttaag cagctggaca tgtcgtgcat gaatcacaag gaaaaaatga acgtcgtgaa cgagctgcgg gcattaatcg aggtgtccgt gcacccattc atcgtgcgat ataaggaagc gttcctggag gacaacattt tgtacgtcgc tatggactac tgctccaaag gagatttaag caaatacata aaaaggtaca aaaaaacgaa cacgttgatt cccgagagga aaataaaaag gtggctgtta cagataatca ctgcgatcaa atttatgcac gacaggaagc ttatacacag ggacttaaaa tgcaacaaca tatttctgga tgatgatgag agggctaagg tgggcgactt tggcctcgcc aaaatttttg agaacacaga acaaacaact acagtgtgtg ggaccattgg gtacatggct ccggagattt gcaggaacgc ggcctacagc ttccccgcgg acatctggtc ccttggcgtg atactgtacg agctcatgag cctccggcac cccttcaagt cggagcactc caacatgttg tcgacggccc agaagatttg cgaagaggag ccagagcccc tgccgacgag ctactcgaac gacctgatac acttgtgcca gtggatgtta aaaaagaact ccgaggaaag gcccacctcg tgcgatatta tctcgaccga ttatttgcag gtgcggtga

Details for PlviB.01209.a.B1.GE37136

HARVESTED ON:	7/17/2013
SEQUENCED ON:	7/22/2013
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	90
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMT TSTSRKIGPY EVVKSIGRGS FGIVTAVKNA QGEIFVVKQL DMSCMNHKEK MNVVNELRAL IEVSVHPFIV RYKEAFLEDN ILYVAMDYCS KGDLSKYIKR YKKTNTLIPE RKIKRWLLQI ITAIKFMHDR KLIHRDLKCN NIFLDDDERA KVGDFGLAKI FENTEQTTTV CGTIGYMAPE ICRNAAYSFP ADIWSLGVIL YELMSLRHPF KSEHSNMLST AQKICEEEVR AAGRLSGKDS QKWRDSGAVV AHQSPCRRAT RTT

Validated NT Sequence

tcttactcac ttaccgcacc tgcaaataat cggtcgagat aatatcgcac gaggtgggcc tttcctcgga gttctttttt aacatccact ggcacaagtg tatcaggtcg ttcgagtagc tcgtcggcag gggctctggt gtgctactac tgcgccacta tcgcgccact tttgtgaatc ttttccgctc agcctccccg ctgcgcgcac ctcctcttcg caaatcttct gggccgtcga caacatgttg gagtgctccg acttgaaggg gtgccggagg ctcatgagct cgtacagtat cacgccaagg gaccagatgt ccgcggggaa gctgtaggcc gcgttcctgc aaatctccgg agccatgtac ccaatggtcc cacacactgt agttgtttgt tctgtgttct caaaaatttt ggcgaggcca aagtcgccca ccttagccct ctcatcatca tccagaaata tgttgttgca ttttaagtcc ctgtgtataa gcttcctgtc gtgcataaat ttgatcgcag tgattatctg taacagccac ctttttattt tcctctcggg aatcaacgtg ttcgtttttt tgtacctttt tatgtatttg cttaaatctc ctttggagca gtagtccata gcgacgtaca aaatgttgtc ctccaggaac gcttccttat atcgcacgat gaatgggtgc acggacacct cgattaatgc ccgtagctcg ttcacgacgt tcattttttc cttgtgattc atgcacgaca tgtccagctg cttaacgacg aagatttccc cctgggcgtt cttcaccgcc gtgacaattc caaatgaccc ccgcccgata gattttacaa cctcataagg acctattttc ctcgatgtag acgtggtcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacca cgtctacatc gaggaaaata ggtccttatg aggttgtaaa atctatcggg cgggggtcat ttggaattgt cacggcggtg aagaacgccc agggggaaat cttcgtcgtt aagcagctgg acatgtcgtg catgaatcac aaggaaaaaa tgaacgtcgt gaacgagctg cgggcattaa tcgaggtgtc cgtgcaccca ttcatcgtgc gatataagga agcgttcctg gaggacaaca ttttgtacgt cgctatggac tactgctcca aaggagattt aagcaaatac ataaaaaggt acaaaaaaac gaacacgttg attcccgaga ggaaaataaa aaggtggctg ttacagataa tcactgcgat caaatttatg cacgacagga agcttataca cagggactta aaatgcaaca acatatttct ggatgatgat gagagggcta aggtgggcga ctttggcctc gccaaaattt ttgagaacac agaacaaaca actacagtgt gtgggaccat tgggtacatg gctccggaga tttgcaggaa cgcggcctac agcttccccg cggacatctg gtcccttggc gtgatactgt acgagctcat gagcctccgg caccccttca agtcggagca ctccaacatg ttgtcgacgg cccagaagat ttgcgaagag gagccagagc ccctgccgac gagctactcg aacgacctga tacacttgtg ccagtggatg ttaaaaaaga actccgagga aaggcccacc tcgtgcgata ttatctcgac cgattatttg caggtgcggt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat