PlviB.00856.a

Ferrochelatase (EC 4.99.1.1)

CENTER ID: PlviB.00856.a
ORGANISM: Plasmodium vivax Salvador I
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlviB.00856.a.B1.GE37777 full length 1 350
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PVX_115205
RefSeq: XP_001616431.1
UniProt: A5K2Q4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIEDFLKFN NLSLSKDKIA NSLQGNKIGI LITNLGSPAK PTYWALYKYL AKFLGDPRVV KLNRFLWLPV LYGYVLPFRS GKSLSKYKNV WTEEGSPLCV NTHNQCAALK ERLFAKYNDK VAITYGMRYG ERSIKRALSY LKGKNINKLL VVPLYPQSAE CTVASTLDCI GENLKNWNNI PELRFLSGYC LNEKYLNSMT ECIQNFWKKN GRGNKLIISY HSIPTKTVED GDLYPFFCIE STNQLIKKLN LRKEDYLLAF QSRIEGQKWI QPCIENILKQ LSSDGCDVVD VVCPSFSADC LETLEEIEIT YRQQFLQYGN GRLRYISCLN YSKVGIDFLM GIIEENMVGW
NT Sequence
atgaatatag aggacttcct aaaattcaac aacttaagct tatccaaaga taaaatagca aacagcctcc aaggcaacaa aatcggcatc ctcataacca atttgggcag ccccgcgaag ccgacttact gggcgctgta caaatatttg gctaagttcc tgggcgaccc gcgagttgtc aagctgaatc gctttttgtg gctgcccgtg ctctacggct acgtactgcc cttccgcagt ggaaagtccc tatccaaata taaaaatgtg tggacagagg aggggtcccc tctatgcgtg aacacgcaca accagtgcgc ggcgctgaag gagcgtctat ttgcaaagta taacgacaaa gttgccatca cctacggaat gcggtacgga gagaggtcca taaaaagggc actgagctat ttgaagggca aaaacataaa caagcttcta gtagtgcccc tctaccccca atcggccgaa tgcacagtgg catccacact tgactgcata ggagaaaatt taaaaaattg gaataacatc ccagagttgc ggttcctatc gggatactgt ctaaatgaaa agtacctaaa cagtatgacc gagtgtattc aaaatttttg gaaaaaaaac ggaaggggaa acaaattaat catatcctac cattccatac ccaccaaaac ggtggaggat ggagatctct atcctttttt ttgtatcgaa agtacaaacc agttgattaa aaagctgaac cttcggaagg aagattacct tttggctttc caatcgagaa tagaaggcca gaaatggata cagccatgta tcgaaaatat tttaaagcag ttgtcttctg atggctgtga tgtggttgat gtggtttgcc catccttttc cgctgactgc ttggagacac tagaagaaat tgaaataacc tatcggcagc agttccttca gtatggcaat ggacgtctgc gatatatcag ctgcttaaat tattccaaag tgggcattga ttttctcatg ggcattattg aggagaacat ggtgggatgg tag
Details for PlviB.00856.a.B1.GE37777
HARVESTED ON: 2/6/2014
SEQUENCED ON: 2/12/2014
EXPECTED MW: 41kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 94
Validated AA Sequence
MAHHHHHHMN IEDFLKFNNL SLSKDKIANS LQGNKIGILI TNLGSPAKPT YWALYKYLAK FLGDPRVVKL NRFLWLPVLY GYVLPFRSGK SLSKYKNVWT EEGSPLCVNT HNQCAALKER LFAKYNDKVA ITYGMRYGER SIKRALSYLK GKNINKLLVV PLYPQSAECT VASTLDCIGE NLKNWNNIPE LRFLSGYCLN EKYLNSMTEC IQNFWKKNGR GNKLIISYHS IPTKTVEDGD LYPFFCIEST NQLIKKLNLR KEDYLLAFQS RIEXQKWIQP CIENILKQLS SDGCDVVDVV CPSFSADCLE TLEEIEITYR QQFPSVMAMD VCXISAA
Validated NT Sequence
cnncnnnnan nnaantttgt ttaactttaa gaaggagata taccatggct caccaccacc accaccatat gaatatagag gacttcctaa aattcaacaa cttaagctta tccaaagata aaatagcaaa cagcctccaa ggcaacaaaa tcggcatcct cataaccaat ttgggcagcc ccgcgaagcc gacttactgg gcgctgtaca aatatttggc taagttcctg ggcgacccgc gagttgtcaa gctgaatcgc tttttgtggc tgcccgtgct ctacggctac gtactgccct tccgcagtgg aaagtcccta tccaaatata aaaatgtgtg gacagaggag gggtcccctc tatgcgtgaa cacgcacaac cagtgcgcgg cgctgaagga gcgtctattt gcaaagtata acgacaaagt tgccatcacc tacggaatgc ggtacggaga gaggtccata aaaagggcac tgagctattt gaagggcaaa aacataaaca agcttctagt agtgcccctc tacccccaat cggccgaatg cacagtggca tccacacttg actgcatagg agaaaattta aaaaattgga ataacatccc agagttgcgg ttcctatcgg gatactgtct aaatgaaaag tacctaaaca gtatgaccga gtgtattcaa aatttttgga aaaaaaacgg aaggggaaac aaattaatca tatcctacca ttccataccc accaaaacgg tggaggatgg agatctctat cctttttttt gtatcgaaag tacaaaccag ttgattaaaa agctgaacct tcgaaaggaa gattaccttt tggctttcca atcgagaata gaaanncaga aatggataca gccatgtatc gaaaatattt taaagcagtt gtcttctgat ggctgtgatg tggttgatgt ggtttgccca tccttttccg ctgactgctt ggagacncta gaagaaattg aaataaccta tcggcagcag ttcccttcag ttatggcaat ggacgtctgc nanatatcag ctgcttaaat tattcccaag tggnattgat tttcnncatg ggcattnatt gagnnacatg nnggnngnna gtgagtaaan nnaggntnnn ctgctaanaa gcccgannnn ncnnnnnngc ngnctgcncc ncctnnnact annnnnnncc nnngnnnnnn nngncngnnt ctnttgaggg cnnnnnnnnn ngnantgtnn ngng
Expected Protein Sequence
MAHHHHHHMN IEDFLKFNNL SLSKDKIANS LQGNKIGILI TNLGSPAKPT YWALYKYLAK FLGDPRVVKL NRFLWLPVLY GYVLPFRSGK SLSKYKNVWT EEGSPLCVNT HNQCAALKER LFAKYNDKVA ITYGMRYGER SIKRALSYLK GKNINKLLVV PLYPQSAECT VASTLDCIGE NLKNWNNIPE LRFLSGYCLN EKYLNSMTEC IQNFWKKNGR GNKLIISYHS IPTKTVEDGD LYPFFCIEST NQLIKKLNLR KEDYLLAFQS RIEGQKWIQP CIENILKQLS SDGCDVVDVV CPSFSADCLE TLEEIEITYR QQFLQYGNGR LRYISCLNYS KVGIDFLMGI IEENMVGW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata tagaggactt cctaaaattc aacaacttaa gcttatccaa agataaaata gcaaacagcc tccaaggcaa caaaatcggc atcctcataa ccaatttggg cagccccgcg aagccgactt actgggcgct gtacaaatat ttggctaagt tcctgggcga cccgcgagtt gtcaagctga atcgcttttt gtggctgccc gtgctctacg gctacgtact gcccttccgc agtggaaagt ccctatccaa atataaaaat gtgtggacag aggaggggtc ccctctatgc gtgaacacgc acaaccagtg cgcggcgctg aaggagcgtc tatttgcaaa gtataacgac aaagttgcca tcacctacgg aatgcggtac ggagagaggt ccataaaaag ggcactgagc tatttgaagg gcaaaaacat aaacaagctt ctagtagtgc ccctctaccc ccaatcggcc gaatgcacag tggcatccac acttgactgc ataggagaaa atttaaaaaa ttggaataac atcccagagt tgcggttcct atcgggatac tgtctaaatg aaaagtacct aaacagtatg accgagtgta ttcaaaattt ttggaaaaaa aacggaaggg gaaacaaatt aatcatatcc taccattcca tacccaccaa aacggtggag gatggagatc tctatccttt tttttgtatc gaaagtacaa accagttgat taaaaagctg aaccttcgga aggaagatta ccttttggct ttccaatcga gaatagaagg ccagaaatgg atacagccat gtatcgaaaa tattttaaag cagttgtctt ctgatggctg tgatgtggtt gatgtggttt gcccatcctt ttccgctgac tgcttggaga cactagaaga aattgaaata acctatcggc agcagttcct tcagtatggc aatggacgtc tgcgatatat cagctgctta aattattcca aagtgggcat tgattttctc atgggcatta ttgaggagaa catggtggga tggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat