PlviB.00214.a

Protein kinase domain containing protein

CENTER ID: PlviB.00214.a
ORGANISM: Plasmodium vivax Salvador I
ASSOCIATED DISEASE: Malaria
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PlviB.00214.a.B1.GE37131 full length 1 348
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PlasmoDB:PVX_122625
RefSeq: XP_001617088.1
UniProt: A5JZF0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCTPISIHRN LLKVAELVKM ECVRKEEPKY KLVKLIGKGT FGKVYYAIDL CTQEPVAIKR SPKWRNKVSR EVDLLQKMRH SENIVNLKSI FYTTTEKGFR IQNIVFKYMT YSLGKYIRFK KQERKENNQI CKGIKHLHEH NLAHRDLKPD NILIDTGSSP FKVEICDLGS AKKVEESIIS IPYICSRWYR APELLCGSMY YTTDVDLWSL GCIIFELINL CPLFPGKFKK DDISEECSQI INLIEVLGSP RMYTVAEETD IWSSRRIDLF EVKSFYESIK GQTSNRNNRL IKELCGLNIR PLSWSSILGD ILEESERELI GIVDGLLKWN PNERLKIDAV LSNPYFAT
NT Sequence
atgtgcacgc cgataagcat acacaggaat cttcttaaag tcgccgagct ggtcaaaatg gaatgcgtca ggaaggaaga gcccaagtac aaattggtga aactcattgg caagggcacc tttgggaagg tctactacgc tattgacctg tgcacgcagg agcccgtcgc gattaagcgg tcccccaagt ggcggaacaa agtctcgagg gaagtcgacc tgctgcagaa gatgcggcac agcgaaaaca tagtaaattt aaaatccatt ttttacacca ccacggagaa gggctttcgt atacagaaca tcgtcttcaa gtacatgacg tacagcctgg gcaagtacat cagatttaag aagcaggagc ggaaggaaaa caaccaaata tgcaaaggga tcaagcacct gcacgagcac aatttagctc acagggattt gaagccggac aacattttga tcgatacagg ctcatcccca tttaaagtgg aaatatgcga tttgggatct gcaaagaaag ttgaagaaag catcatctcg attccgtaca tctgctcgag gtggtaccgg gcacctgagt tgctgtgcgg ctccatgtat tacacgacgg acgtagacct atggtccctg gggtgcataa ttttcgagct gataaacctc tgcccgctgt tccccgggaa gttcaaaaag gacgacatat ccgaagaatg ctcacaaatt ataaatttaa ttgaagtgct gggatccccc cgaatgtata ccgtcgcgga agagacagac atttggtcgt cacgacgaat cgatttattc gaagtgaaga gtttttacga aagtataaag ggccaaacta gtaacaggaa caatcgcctc atcaaggaac tgtgcggact gaacattcgg cccctgtcct ggagctccat cctgggggac atcttggagg aaagtgaacg ggagctaatc ggcatcgtag atggcctttt gaaatggaat ccaaatgaaa ggttaaaaat tgacgcagtt ttgagtaacc cttactttgc tacatga
Details for PlviB.00214.a.B1.GE37131
HARVESTED ON: 7/17/2013
SEQUENCED ON: 7/20/2013
EXPECTED MW: 41kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 96
PERCENT COVERAGE: 89
Validated AA Sequence
MAHHHHHHMC TPISIHRNLL KVAELVKMEC VRKEEPKYKL VKLIGKGTFG KVYYAIDLCT QEPVAIKRSP KWRNKVSREV DLLQKMRHSE NIVNLKSIFY TTTEKGFRIQ NIVFKYMTYS LGKYIRFKKQ ERKENKKDRI APSDLKIIIY QICKGIKHLH EHNLAHRDLK PDNILIDTGS SPFKVEICDL GSAKKVEESI ISIPYICSRW YRAPELLCGS MYYTTDVDLW SLGCIIFELI NLCPLFPGKF KKDDISEECS QIINLIEVLG SPRMSFYESI KGQTSNXNNR LIKELCGLNI XPLSWXSILG XXLXXXNGX
Validated NT Sequence
attttgntta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgtgc acgccgataa gcatacacag gaatcttctt aaagtcgccg agctggtcaa aatggaatgc gtcaggaagg aagagcccaa gtacaaattg gtgaaactca ttggcaaggg cacctttggg aaggtctact acgctattga cctgtgcacg caggagcccg tcgcgattaa gcggtccccc aagtggcgga acaaagtctc gagggaagtc gacctgctgc agaagatgcg gcacagcgaa aacatagtaa atttaaaatc cattttttac accaccacgg agaagggctt tcgtatacag aacatcgtct tcaagtacat gacgtacagc ctgggcaagt acatcagatt taagaagcag gagcggaagg aaaacaagaa agaccgcatt gcaccgtctg acttaaaaat catcatatac caaatatgca aagggatcaa gcacctgcac gagcacaatt tagctcacag ggatttgaag ccggacaaca ttttgatcga tacaggctca tccccattta aagtggaaat atgcgatttg ggatctgcaa agaaagttga agaaagcatc atctcgattc cgtacatctg ctcgaggtgg taccgggcac ctgagttgct gtgcggctcc atgtattaca cgacggacgt agacctatgg tccctggggt gcataatttt cgagctgata aacctctgcc cgctgttccc cgggaagttc aaaaaggacg acatatccga agaatgctca caaattataa atttaattga agtgctggga tccccccgaa tgagttttta cgaaagtata aagggccaaa ctagtaacng gaacaatcgc ctcatcaagg aactgtgcgg actgaacatt cngcccctgt cctgganctc catcctgggg ganntcttgg nngannngaa cgggnnctaa tcggcntcgn ananggnntt ttgaatggan cnntgnangt aaaannacnc antttngann ancnnnnnnn tannnnannn nntannannn nncnnnnnnn nnnna
Expected Protein Sequence
MAHHHHHHMC TPISIHRNLL KVAELVKMEC VRKEEPKYKL VKLIGKGTFG KVYYAIDLCT QEPVAIKRSP KWRNKVSREV DLLQKMRHSE NIVNLKSIFY TTTEKGFRIQ NIVFKYMTYS LGKYIRFKKQ ERKENNQICK GIKHLHEHNL AHRDLKPDNI LIDTGSSPFK VEICDLGSAK KVEESIISIP YICSRWYRAP ELLCGSMYYT TDVDLWSLGC IIFELINLCP LFPGKFKKDD ISEECSQIIN LIEVLGSPRM YTVAEETDIW SSRRIDLFEV KSFYESIKGQ TSNRNNRLIK ELCGLNIRPL SWSSILGDIL EESERELIGI VDGLLKWNPN ERLKIDAVLS NPYFAT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgca cgccgataag catacacagg aatcttctta aagtcgccga gctggtcaaa atggaatgcg tcaggaagga agagcccaag tacaaattgg tgaaactcat tggcaagggc acctttggga aggtctacta cgctattgac ctgtgcacgc aggagcccgt cgcgattaag cggtccccca agtggcggaa caaagtctcg agggaagtcg acctgctgca gaagatgcgg cacagcgaaa acatagtaaa tttaaaatcc attttttaca ccaccacgga gaagggcttt cgtatacaga acatcgtctt caagtacatg acgtacagcc tgggcaagta catcagattt aagaagcagg agcggaagga aaacaaccaa atatgcaaag ggatcaagca cctgcacgag cacaatttag ctcacaggga tttgaagccg gacaacattt tgatcgatac aggctcatcc ccatttaaag tggaaatatg cgatttggga tctgcaaaga aagttgaaga aagcatcatc tcgattccgt acatctgctc gaggtggtac cgggcacctg agttgctgtg cggctccatg tattacacga cggacgtaga cctatggtcc ctggggtgca taattttcga gctgataaac ctctgcccgc tgttccccgg gaagttcaaa aaggacgaca tatccgaaga atgctcacaa attataaatt taattgaagt gctgggatcc ccccgaatgt ataccgtcgc ggaagagaca gacatttggt cgtcacgacg aatcgattta ttcgaagtga agagttttta cgaaagtata aagggccaaa ctagtaacag gaacaatcgc ctcatcaagg aactgtgcgg actgaacatt cggcccctgt cctggagctc catcctgggg gacatcttgg aggaaagtga acgggagcta atcggcatcg tagatggcct tttgaaatgg aatccaaatg aaaggttaaa aattgacgca gttttgagta acccttactt tgctacatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at